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OfflinePsyCLown89
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Re: Pasty Agar Tek [Re: taGyo]
    #20982993 - 12/16/14 02:05 AM (9 years, 1 month ago)

Quote:

taGyo said:
So I had 4 APE syringes that had bacteria. Only one germinated myc and it's in a pool of bacteria. I intend to make a transfer when it makes it's way out but for now it's just kinda chillin'.

Will these syringes NEVER germinate? I got my replacements and I'm kind of low on syringe space atm so should I toss the other or if I mailed them to some of my friends would they be able to clean it up on agar eventually?




This is interesting and might be what I am now experiencing - my B+ syringe I got and used to inoc my BRF cakes worked fine. I put it back into the fridge for a few weeks and now I cannot get it to germinate on agar at all?

Possibly due to bacteria? Bacteria might prevent spores from germinating?
What if you scraped from spores from a dirty print onto some agar, would that have a higher chance of germinating then?


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: PsyCLown89]
    #20983330 - 12/16/14 06:30 AM (9 years, 1 month ago)

Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.


--------------------
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OfflinePsyCLown89
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Re: Pasty Agar Tek [Re: taGyo]
    #20983825 - 12/16/14 09:52 AM (9 years, 1 month ago)

Quote:

taGyo said:
Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.




I know for a fact that my syringe spores were active though - I used it to inoculate my BRF cakes just over a month ago.
Now that I am trying to get some growing on agar from the exact same syringe I am not have any luck. I successfully moved some myc from a BRF cake to agar and that it taking well to the agar.

This is a bit off topic, but would a spore print last longer than a syringe if both were kept in their ideal temperature range?

If you're going to just give away an APE syringe I will happily take one! Partial print or swab even! :P


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"It's all in your head"
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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: PsyCLown89]
    #20983892 - 12/16/14 10:11 AM (9 years, 1 month ago)

Quote:

PsyCLown89 said:
Quote:

taGyo said:
Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.




I know for a fact that my syringe spores were active though - I used it to inoculate my BRF cakes just over a month ago.
Now that I am trying to get some growing on agar from the exact same syringe I am not have any luck. I successfully moved some myc from a BRF cake to agar and that it taking well to the agar.

This is a bit off topic, but would a spore print last longer than a syringe if both were kept in their ideal temperature range?

If you're going to just give away an APE syringe I will happily take one! Partial print or swab even! :P




I have never had any luck with spore water to agar. If i were you I would use that spore syringe on some grain jars and then use individual colonized grain kernels to grow out on agar. This method works like a charm. You could either save the rest of the grain jar and store it in a lunch box in the crisper or spawn it to a mini bulk grow.


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OfflinePsyCLown89
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Re: Pasty Agar Tek [Re: insanemike]
    #20983963 - 12/16/14 10:28 AM (9 years, 1 month ago)

Quote:

insanemike said:
Quote:

PsyCLown89 said:
Quote:

taGyo said:
Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.




I know for a fact that my syringe spores were active though - I used it to inoculate my BRF cakes just over a month ago.
Now that I am trying to get some growing on agar from the exact same syringe I am not have any luck. I successfully moved some myc from a BRF cake to agar and that it taking well to the agar.

This is a bit off topic, but would a spore print last longer than a syringe if both were kept in their ideal temperature range?

If you're going to just give away an APE syringe I will happily take one! Partial print or swab even! :P




I have never had any luck with spore water to agar. If i were you I would use that spore syringe on some grain jars and then use individual colonized grain kernels to grow out on agar. This method works like a charm. You could either save the rest of the grain jar and store it in a lunch box in the crisper or spawn it to a mini bulk grow.




Thats a bit more reassuring although a bit disappointing. I wonder why?
Seems as if others have had no issues with it. HmmZ.

At the moment I do not really have much of a need for the syringe. I am hoping to get some other "strains" and get those growing as soon as they arrive.
I have some B+ myc growing on my agar and my B+ BRF cakes will be put into fruiting quite soon.

It has only been 4 or so days since I have inoculated the new agar with some syringe drops and some myc from a BRF cake.
The Myc has really taken to the agar. Perhaps the MS syringe will decide it likes agar and decide to show its face.


--------------------
My Trade List - Click ME!!   [Updated: 02/05/2015]




"It's all in your head"
"Haste makes waste!"


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: PsyCLown89]
    #20984108 - 12/16/14 11:13 AM (9 years, 1 month ago)

Quote:

PsyCLown89 said:
Quote:

insanemike said:
Quote:

PsyCLown89 said:
Quote:

taGyo said:
Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.




I know for a fact that my syringe spores were active though - I used it to inoculate my BRF cakes just over a month ago.
Now that I am trying to get some growing on agar from the exact same syringe I am not have any luck. I successfully moved some myc from a BRF cake to agar and that it taking well to the agar.

This is a bit off topic, but would a spore print last longer than a syringe if both were kept in their ideal temperature range?

If you're going to just give away an APE syringe I will happily take one! Partial print or swab even! :P




I have never had any luck with spore water to agar. If i were you I would use that spore syringe on some grain jars and then use individual colonized grain kernels to grow out on agar. This method works like a charm. You could either save the rest of the grain jar and store it in a lunch box in the crisper or spawn it to a mini bulk grow.




Thats a bit more reassuring although a bit disappointing. I wonder why?
Seems as if others have had no issues with it. HmmZ.

At the moment I do not really have much of a need for the syringe. I am hoping to get some other "strains" and get those growing as soon as they arrive.
I have some B+ myc growing on my agar and my B+ BRF cakes will be put into fruiting quite soon.

It has only been 4 or so days since I have inoculated the new agar with some syringe drops and some myc from a BRF cake.
The Myc has really taken to the agar. Perhaps the MS syringe will decide it likes agar and decide to show its face.




My problem with the spore syringe to agar is mainly due to the water in the spore syringe. Too much water will support bacterial growth maybe even before the spores have had a chance to germinate.


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OfflineHierophant
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Registered: 03/28/13
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Re: Pasty Agar Tek [Re: taGyo]
    #20987857 - 12/17/14 03:03 AM (9 years, 1 month ago)

Quote:

taGyo said:
Yeah,
Scraping would work better if your syringe isn't working 'cause at least you know those spores are active. I'm gonna test 'em all out and if I have a few extra APE syringes I'll give them away.




Please send one my way brother or a print if possible... or jus reg PE, I could offer an Amazonian print in exchange.

Peace


--------------------
There is a golden book kept in my heart and guarded by my soul, written in Divine Light and bound with the veins of the Earth.


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OfflineBUK
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Registered: 11/03/14
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Re: Pasty Agar Tek [Re: PsyCLown89]
    #20987868 - 12/17/14 03:15 AM (9 years, 1 month ago)

Quote:

PsyCLown89 said:
Quote:

insanemike said:
I have never had any luck with spore water to agar. If i were you I would use that spore syringe on some grain jars and then use individual colonized grain kernels to grow out on agar.




Thats a bit more reassuring although a bit disappointing. I wonder why?
Seems as if others have had no issues with it. HmmZ.





No, I completely agree. Swiping a spore print onto agar is fine but if it's in solution I'd do exactly as Mike says. Even better than extracting a grain, with sclerotia producers, is taking a baby in-vitro sclerotium. That seems to be working really well for me.


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InvisibleFrankHorrigan
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Registered: 01/04/11
Posts: 10,573
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Re: Pasty Agar Tek [Re: insanemike]
    #20989810 - 12/17/14 02:51 PM (9 years, 1 month ago)

Quote:

insanemike said:
My problem with the spore syringe to agar is mainly due to the water in the spore syringe. Too much water will support bacterial growth maybe even before the spores have had a chance to germinate.




Wrong. Water doesn't cause bacteria, bacteria causes bacteria.

Apply one to three drops of spore solution per plate. If you're not using a petri dish, the same steps apply here.

Leave it be for 24 hours (I just leave it in the SAB, no wrapping).

The water will be absorbed by the agar.

Then wrap your dish if you're using one.

If bacteria grows and no mycelium comes of it, your syringe is bad or your inoculation technique is awful.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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InvisibleNumeroEno
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Registered: 07/24/14
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20989865 - 12/17/14 03:03 PM (9 years, 1 month ago)

Quote:

FrankHorrigan said:
Quote:

insanemike said:
My problem with the spore syringe to agar is mainly due to the water in the spore syringe. Too much water will support bacterial growth maybe even before the spores have had a chance to germinate.




Wrong. Water doesn't cause bacteria, bacteria causes bacteria.

Apply one to three drops of spore solution per plate. If you're not using a petri dish, the same steps apply here.

Leave it be for 24 hours (I just leave it in the SAB, no wrapping).

The water will be absorbed by the agar.

Then wrap your dish if you're using one.

If bacteria grows and no mycelium comes of it, your syringe is bad or your inoculation technique is awful.




I've had some trouble lately with spores just not germinating on agar. I've tried streaking with a loop and making syringes. What are the common reasons for spores just not germinating on agar?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

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InvisibleFrankHorrigan
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Registered: 01/04/11
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Re: Pasty Agar Tek [Re: NumeroEno]
    #20989887 - 12/17/14 03:09 PM (9 years, 1 month ago)

Have you tried a different print? What agar recipe are you using?

IME, usually the spores just aren't viable for some reason or another.

Sometimes the spores will need to rehydrate a bit, tho this may not be the case since you have tried making a syringe.

You can rehydrate the spores from a print by placing a drop of sterile water on your agar, then swiping the spores into it.

I successfully "revived" some older prints that way.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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InvisibleNumeroEno
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20989927 - 12/17/14 03:18 PM (9 years, 1 month ago)

Quote:

FrankHorrigan said:
Have you tried a different print? What agar recipe are you using?

IME, usually the spores just aren't viable for some reason or another.

Sometimes the spores will need to rehydrate a bit, tho this may not be the case since you have tried making a syringe.

You can rehydrate the spores from a print by placing a drop of sterile water on your agar, then swiping the spores into it.

I successfully "revived" some older prints that way.




I've been using PDA, but I don't really have a firm recipe down. I start with a pint of water, 2 teaspoons of dextrose, and one teaspoon of potato flakes, then I add agar powder in small amounts and simmer it down until I get it to the right consistency.

I have a purple mystic print and a P. allenii print and I haven't gotten either to germinate. It would suck if these spores are no good. I wanted to grow them out. I especially wanted to take a stab at growing the allenii outdoors. I got these prints in trades so I didn't spend money on them or anything, but it has been kinda frustrating not being able to germinate them.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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InvisibleFrankHorrigan
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Re: Pasty Agar Tek [Re: NumeroEno]
    #20989954 - 12/17/14 03:25 PM (9 years, 1 month ago)

Have you looked at my agar recipe list? It needs to be completed, but the main cube growing recipes are all there and work great for me.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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OfflinetaGyo
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20989959 - 12/17/14 03:26 PM (9 years, 1 month ago)

So I read snake venom in agar makes the the cell walls dissolve and the genes mix, thus creating a new variety.


--------------------
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TNF Q&A :rockon:
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InvisibleNumeroEno
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Registered: 07/24/14
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20989964 - 12/17/14 03:28 PM (9 years, 1 month ago)

Quote:

FrankHorrigan said:
Have you looked at my agar recipe list? It needs to be completed, but the main cube growing recipes are all there and work great for me.




I just found it. I'm gonna read it all the way through before making my next batch of agar :cheers:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: taGyo]
    #20989969 - 12/17/14 03:29 PM (9 years, 1 month ago)

venom is what they use to crossbreed, yes. I believe RR has done it. try looking up some posts from him.


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InvisibleNumeroEno
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Re: Pasty Agar Tek [Re: Munchauzen]
    #20989987 - 12/17/14 03:31 PM (9 years, 1 month ago)

Frank, what kind of yeast do you use in the PDYA? Would active dry yeast for baking or nutritional yeast flakes work better?


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisibleFrankHorrigan
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Re: Pasty Agar Tek [Re: taGyo]
    #20990088 - 12/17/14 03:52 PM (9 years, 1 month ago)

Quote:

EnoRoxxx said:
Frank, what kind of yeast do you use in the PDYA? Would active dry yeast for baking or nutritional yeast flakes work better?




Use nutritional yeast.

Quote:

taGyo said:
So I read snake venom in agar makes the the cell walls dissolve and the genes mix, thus creating a new variety.




You can try your hand at making your own "variety" by simply printing one cap of one variety on top of a print from a different variety, then swiping those spores on to a plate and looking for novel mushrooms to clone/print come harvest time. Don't expect the results to be too exciting though, since mutant varieties like PE will not readily mix with other phenotypes.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20990098 - 12/17/14 03:55 PM (9 years, 1 month ago)

That easy huh?
I'll give it a shot with some GT and KSSS :yesnod:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
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Re: Pasty Agar Tek [Re: taGyo]
    #20990190 - 12/17/14 04:12 PM (9 years, 1 month ago)

If I ever get my PM spore print to germinate, I thought mixing PM and AA+ spawn would be interesting. However, I doubt it would get me a leucistic PM.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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