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InvisiblePsillyDoctor
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Registered: 10/23/14
Posts: 174
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20973613 - 12/14/14 12:44 AM (9 years, 1 month ago)

im using straight black. was gonna do 10 drops thanks for the info. was not sure if i should do more then 10 now i know that would be a over kill. how many use polyfil packed tight?


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InvisibleBig Bear
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Registered: 06/11/14
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Re: Pasty Agar Tek [Re: PsillyDoctor]
    #20973693 - 12/14/14 01:34 AM (9 years, 1 month ago)

Does the food color make it easier for y'all to see?


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Big Bear]
    #20974107 - 12/14/14 07:05 AM (9 years, 1 month ago)

Yes except for black. I have some on black agar and they work fine but there is no benefit.  Contams appear more easily on a colored surface.  That or, none of my black plates have contams but the surface still looks weird because it's black.

Either way, the food coloring is good.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: Inocuole]
    #20974167 - 12/14/14 07:42 AM (9 years, 1 month ago)

What kind of contams are we trying to see exactly? I've never used food coloring for agar before but this has my interest. I have seen my share of contams on agar plates. The only one I have not been able to see is bacteria but that's not hard to determine when growth comes to a halt. Smell is usually another give away for bacterial contamination. So is there something that I'm missing?


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: insanemike]
    #20974172 - 12/14/14 07:44 AM (9 years, 1 month ago)

I've heard that the food coloring doesn't show bacteria any better as well, just the myc itself and the growth in general. I just use the red chunks of agar so I already have a decently colored plate to use. Smell is what I usually use.


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InvisibleImperfect Iam
^means imperfect,not I'm perfect
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20974352 - 12/14/14 08:50 AM (9 years, 1 month ago)

I have never used food coloring, just because that is something I don't just regularly have around the house, but I need to remember to get some, next time I am at the grocery store.

It's not like agar or malt extract is something I USED to keep around the house regularly either, but I just got mostly all the stuff almost def. needed for this hobby. Now I can start picking up specialty items.

It's not like it does not work right w/o the food coloring, but I think it would be very cool to find rhe best colors that show contams better (.any hints) than just the regular tannish looking agsr


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Invisiblewowimflabbergasted
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Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20974377 - 12/14/14 09:00 AM (9 years, 1 month ago)

I like using a dark blue or green.


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20974620 - 12/14/14 10:07 AM (9 years, 1 month ago)

Quote:

Imperfect Iam said:
I have never used food coloring, just because that is something I don't just regularly have around the house, but I need to remember to get some, next time I am at the grocery store.

It's not like agar or malt extract is something I USED to keep around the house regularly either, but I just got mostly all the stuff almost def. needed for this hobby. Now I can start picking up specialty items.

It's not like it does not work right w/o the food coloring, but I think it would be very cool to find rhe best colors that show contams better (.any hints) than just the regular tannish looking agsr




So it's just for entertainment purposes? :mindexpanding:


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OfflineHierophant
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20974833 - 12/14/14 10:56 AM (9 years, 1 month ago)

Quote:

Elf_on_a_Log said:
Quote:

Hierophant said:
I'm wanting to make a liquid inoculate but I don't think one plate will be enough for 9 quarts



A single plate will easily make enough for 9 quarts.  Not long ago I did a single plate LI with a 12cc syringe and inoculated 10 quarts with it with some LI to spare.  Shook the hell out of the grain jars right after inoculation and they were ready for a second shake within a week.  After the second shake they were fully colonized within 3-5 days.

Definitely do a transfer though if you don't have any other plates of it.  It's always nice to have some backups in case of contam issues.




Thanks, this was helpful. How did you make your LI? and why did you use a syringe as opposed to just pouring?


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There is a golden book kept in my heart and guarded by my soul, written in Divine Light and bound with the veins of the Earth.


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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: Hierophant]
    #20975155 - 12/14/14 12:17 PM (9 years, 1 month ago)

Quote:

Hierophant said:
Thanks, this was helpful. How did you make your LI? and why did you use a syringe as opposed to just pouring?



I make mine in what I guess is a kind of unique way.  I think it's MudaFuka that posted the tek on using a blender and a jar to make LI.  I use that idea to some degree but I don't have a blender.

What I do is I take a PC'd half pint of water with a hole in the lid and use a syringe to suck up water from it.  Then, since a pasty plate has a hole in the lid, I peel the micropore tape partially off of the hole (being careful to not let the hole become exposed to potential falling contams in my SAB) and I inject the water from the syringe into the pasty plate.

Then I take a flame sterilized inoculation loop and stick it into the hole and massage the surface of the agar until a good majority of the mycelium is scraped off and suspended in the water.  After that I like to twist the inoculation loop around in the plate for a minute or so by rolling the handle between my fingers.  This helps to break up the agar to some small degree, loosening even more mycelium (ghetto rigged blender action.)  Then I swirl the water around in the pasty plate for a minute or so and suck it back up into the syringe, and then shake the crap out of the syringe to disperse the myc into the water even further.

Usually there's still a fairly large round of agar in the plate, but all the inoculation loop action and water movement has gotten enough mycelium dispersed into the water to make a good LI out of it.

It's worth noting that the first time I did this I used a normal inoculation loop and it was a bit too flimsy to do the job efficiently, so I made a customized double thick, double long loop specifically to do this.

Edit:  Here's Muda's Liquid Inoculate tek I was referring to.


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"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Edited by Elf_on_a_Log (12/14/14 12:34 PM)


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OfflinePsyCLown89
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Registered: 08/18/14
Posts: 789
Loc: South Africa Flag
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20975282 - 12/14/14 12:47 PM (9 years, 1 month ago)

Quote:

Elf_on_a_Log said:
Quote:

Hierophant said:
Thanks, this was helpful. How did you make your LI? and why did you use a syringe as opposed to just pouring?



I make mine in what I guess is a kind of unique way.  I think it's MudaFuka that posted the tek on using a blender and a jar to make LI.  I use that idea to some degree but I don't have a blender.

What I do is I take a PC'd half pint of water with a hole in the lid and use a syringe to suck up water from it.  Then, since a pasty plate has a hole in the lid, I peel the micropore tape partially off of the hole (being careful to not let the hole become exposed to potential falling contams in my SAB) and I inject the water from the syringe into the pasty plate.

Then I take a flame sterilized inoculation loop and stick it into the hole and massage the surface of the agar until a good majority of the mycelium is scraped off and suspended in the water.  After that I like to twist the inoculation loop around in the plate for a minute or so by rolling the handle between my fingers.  This helps to break up the agar to some small degree, loosening even more mycelium (ghetto rigged blender action.)  Then I swirl the water around in the pasty plate for a minute or so and suck it back up into the syringe, and then shake the crap out of the syringe to disperse the myc into the water even further.

Usually there's still a fairly large round of agar in the plate, but all the inoculation loop action and water movement has gotten enough mycelium dispersed into the water to make a good LI out of it.

It's worth noting that the first time I did this I used a normal inoculation loop and it was a bit too flimsy to do the job efficiently, so I made a customized double thick, double long loop specifically to do this.

Edit:  Here's Muda's Liquid Inoculate tek I was referring to.




That is pretty interesting.
Sounds a lot easier than trying to find a blender and spending money on a blender which I probably not use for anything other than making LI etc.

I think I will give this a try sometime soon. Got some B+ growing on agar just to get the hang of it while I wait for some prints to arrive. Might as well give it a shot and see whether it works or not. Possibly try and colonize some wbs and get playing with some grains.

I wonder whether you could mix 1 jar of B+ with jars of other "strains" in a spawn? I'd imagine it would work and the end result would be a mixture of "strains" when it starts to fruit.

ie. if you had some APE and some Redboy jars spawned to the same bulk substrate you would find both types of mushrooms growing?


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OfflinetaGyo
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Registered: 10/16/14
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Re: Pasty Agar Tek [Re: PsyCLown89]
    #20975704 - 12/14/14 02:43 PM (9 years, 1 month ago)

On the note of food coloring I just used turquoise and it fucking sucks.

Can't see shit.


--------------------
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OfflineHierophant
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Re: Pasty Agar Tek [Re: taGyo]
    #20975826 - 12/14/14 03:11 PM (9 years, 1 month ago)

Thanks Elf, interesting method


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There is a golden book kept in my heart and guarded by my soul, written in Divine Light and bound with the veins of the Earth.


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Offlinetombosley8
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Registered: 10/14/13
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Re: Pasty Agar Tek [Re: Hierophant]
    #20976405 - 12/14/14 05:44 PM (9 years, 1 month ago)

i'd recommended getting the blender. A lot less risk and 100% efficient. I love making a machine do my work for me. Get the cheapo oster blender and silicon glue the jar ring to the blender attachment and wala. Not hard at all. If you really don't have the resources then resort to a method similar to the scraping and breaking up with the 'noc loop. Also iv;e heard a shard of broken glass or similar can work by shaking profusely.


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Edited by tombosley8 (12/14/14 05:46 PM)


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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: tombosley8]
    #20976468 - 12/14/14 06:02 PM (9 years, 1 month ago)

Quote:

tombosley8 said:
i'd recommended getting the blender. A lot less risk and 100% efficient.



Yeah I agree, Muda's blender tek is far superior to my method.  Just dropping a wedge into a jar and shaking it is probably a lot safer as well.  I've only made LI a few times so far just to test the differences in inoculation methods and colonization speeds from those methods, not enough to justify spending the money on a blender yet.  Might do that in the future though.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


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Offlinetombosley8
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20976486 - 12/14/14 06:08 PM (9 years, 1 month ago)

got my blender from wally world for $15 just to put that out there


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OfflinePsyCLown89
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Registered: 08/18/14
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Re: Pasty Agar Tek [Re: tombosley8]
    #20978124 - 12/15/14 04:55 AM (9 years, 1 month ago)

You guys are all overseas - mostly in America I think.
Have some awesome specials and lots of products we don't get here in South Africa =/

Canning jars is a brilliant example. Nobody here know what to y are, I didn't even know about them until this forum. Nobody here does that stuff.
Sigh

I am still interested in trying the LI method without a blender. :laugh:


--------------------
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"It's all in your head"
"Haste makes waste!"


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: PsyCLown89] * 1
    #20979350 - 12/15/14 12:22 PM (9 years, 1 month ago)

Here are the 5 plates I put a chunk of mushroom tissue on about 10 days ago or so. Everything looking great?



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InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: stareatclouds]
    #20979382 - 12/15/14 12:28 PM (9 years, 1 month ago)

Quote:

stareatclouds said:
Here are the 5 plates I put a chunk of mushroom tissue on about 10 days ago or so. Everything looking great?





Looking good!  :thumbup:

Plate 5 looks like what appears to be a satellite at about 7 o'clock but it's hard to tell from the pic.  I'd take wedges or transfers from plates 3 and 4 if it were me.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20980156 - 12/15/14 03:23 PM (9 years, 1 month ago)

So I had 4 APE syringes that had bacteria. Only one germinated myc and it's in a pool of bacteria. I intend to make a transfer when it makes it's way out but for now it's just kinda chillin'.

Will these syringes NEVER germinate? I got my replacements and I'm kind of low on syringe space atm so should I toss the other or if I mailed them to some of my friends would they be able to clean it up on agar eventually?


--------------------
Gyo's Better Grows
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Dominus fortunae meae sum


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