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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20960222 - 12/11/14 12:03 AM (9 years, 1 month ago)

You may as well transfer each of them to a new dish, and then use the rest of it to either make separate LIs or just inoculate grain with wedges.  Watch it all closely, of course.

Put them all to grain though, why not?  If it turns out clean I bet you'll be 10x more confident next time you have a plate that looks like that after being inoculated with a liquid.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Inocuole]
    #20960235 - 12/11/14 12:07 AM (9 years, 1 month ago)

Yeah, I'll do something with the clean looking ones. I probably won't make transfers because I have so much of that LC already and I want to start messing with other strains. Plus I can bring them back with tissue from the fruits they'll produce. Thanks, iNoc.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20960255 - 12/11/14 12:12 AM (9 years, 1 month ago)

You could also tiger drop em. :shrug:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflinePsyCLown89
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Re: Pasty Agar Tek [Re: Inocuole]
    #20961843 - 12/11/14 12:25 PM (9 years, 1 month ago)

So I decided to make some fresh agar on Tuesday and then PC'd it that night.

Today I decided to play around a bit more. So I took the one BRF cake I had which had some contams near the bottom which the myc had grown over and I opened it, scraped away the verm layer and then got some myc from the top on the opposite side of where the contam was and then dropped a piece into two agar plates.

So lets see what happens and how clean it turns out. So far I have had nothing but a bit of contams grow on agar. That was when I scraped some gills from dry shrooms I got from someone to see whether anything might grow.

I then dropped some water from a MS syringe on a third agar plate to see if maybe this time I shall get lucky enough to have something grow there.

So lets see what happens, I am starting to wonder whether I am making my agar right. Perhaps too thick and jelly like?

Fingers crossed I can get some myc growing on the agar this time!!
Getting a bit worried whether I will be able to get some myc growing on new agar plates when I receive some more prints which have been mailed. :frown:


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InvisibleElf_on_a_Log
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Re: Pasty Agar Tek [Re: PsyCLown89]
    #20966046 - 12/12/14 10:02 AM (9 years, 1 month ago)

How long is mycelium still viable once it's leeched all the color out of an agar plate?  Say for instance it's stored at room temperature and it leeched all the color out of the agar a week to two weeks ago.  If I were to make an LI out of the dish would it take off if put to grains?  Am I correct in assuming that it would remain viable until the agar completely dries out?

I could easily do transfers to test this but I just made grain jars I'm planning to inoculate today and I've got plates like that I'm considering using for them so I thought I'd ask before committing.


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"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
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Invisiblewowimflabbergasted
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20966055 - 12/12/14 10:05 AM (9 years, 1 month ago)

Quote:

Elf_on_a_Log said:
How long is mycelium still viable once it's leeched all the color out of an agar plate?  Say for instance it's stored at room temperature and it leeched all the color out of the agar a week to two weeks ago.  If I were to make an LI out of the dish would it take off if put to grains?  Am I correct in assuming that it would remain viable until the agar completely dries out?

I could easily do transfers to test this but I just made grain jars I'm planning to inoculate today and I've got plates like that I'm considering using for them so I thought I'd ask before committing.



:thumbup:


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20966057 - 12/12/14 10:05 AM (9 years, 1 month ago)

Not sure if this helps but I had some LCs I left at room temperature for over a month once I'd extracted a little bit up. They never recovered, just kind of floated there separately.

I must have done these back in September or very early Novemeber. I just sucked them up into my syringe and shot them onto grains and jars and they picked up right where they left off so I think you're good.


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InvisibleElf_on_a_Log
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Re: Pasty Agar Tek [Re: taGyo]
    #20966127 - 12/12/14 10:19 AM (9 years, 1 month ago)

Awesome, thanks for the quick response.  That alleviates some worry about potentially wasting 20 oat jars that took 3 days to prep.


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"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20966138 - 12/12/14 10:22 AM (9 years, 1 month ago)

:cookiemonster:


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Gyo's Better Grows
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Invisibleshroominmyroom
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Re: Pasty Agar Tek [Re: taGyo]
    #20967049 - 12/12/14 02:24 PM (9 years, 1 month ago)

:leaving:


Edited by shroominmyroom (07/31/17 11:32 AM)


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: shroominmyroom]
    #20967056 - 12/12/14 02:25 PM (9 years, 1 month ago)

Looks like it touched the side of the PC.


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Invisibleshroominmyroom
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Re: Pasty Agar Tek *DELETED* [Re: MudaFuka]
    #20967075 - 12/12/14 02:30 PM (9 years, 1 month ago)

:flyhigh:


Edited by shroominmyroom (07/31/17 11:20 AM)


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OfflineHierophant
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Re: Pasty Agar Tek [Re: shroominmyroom]
    #20969030 - 12/12/14 10:08 PM (9 years, 1 month ago)

The myc in my first plate has fully colonized the surface of the agar, I know I should have started transfers already but life has been busy. Is it safe to transfer from the sides? Should I take sections closer to the center or even the original tissue sample I started with and transfer that to new plates? I'm wanting to amass enough myc to colonize 9 jars for Muda's Bottle Tek, any suggestions would be much appreciated my friends.


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Hierophant]
    #20969054 - 12/12/14 10:14 PM (9 years, 1 month ago)

Quote:

Hierophant said:
Is it safe to transfer from the sides? Should I take sections closer to the center



:takingnotes:


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: taGyo]
    #20970389 - 12/13/14 09:10 AM (9 years, 1 month ago)

If your plan was to make LI with it then use the whole plate. If you were going to transfer it then take from the edge.


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OfflineHierophant
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20970937 - 12/13/14 11:50 AM (9 years, 1 month ago)

Thanks, yeah I'm wanting to make a liquid inoculate but I don't think one plate will be enough for 9 quarts so I want to transfer tissue from this plate to others. Guessing you take from the edge cause it's where the fastest growth is occurring?


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There is a golden book kept in my heart and guarded by my soul, written in Divine Light and bound with the veins of the Earth.


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InvisibleElf_on_a_Log
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Re: Pasty Agar Tek [Re: Hierophant]
    #20971211 - 12/13/14 01:03 PM (9 years, 1 month ago)

Quote:

Hierophant said:
I'm wanting to make a liquid inoculate but I don't think one plate will be enough for 9 quarts



A single plate will easily make enough for 9 quarts.  Not long ago I did a single plate LI with a 12cc syringe and inoculated 10 quarts with it with some LI to spare.  Shook the hell out of the grain jars right after inoculation and they were ready for a second shake within a week.  After the second shake they were fully colonized within 3-5 days.

Definitely do a transfer though if you don't have any other plates of it.  It's always nice to have some backups in case of contam issues.


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"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



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InvisiblePsillyDoctor
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Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20973309 - 12/13/14 10:26 PM (9 years, 1 month ago)

how many drop of food coloring would one use for 20 plates? doing up some agar for John Allen  tomorrow.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: PsillyDoctor]
    #20973322 - 12/13/14 10:34 PM (9 years, 1 month ago)

just one or two drops should be fine I would think.  Just add color until it's the color you want.  :shrug:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisibleElf_on_a_Log
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Re: Pasty Agar Tek [Re: Inocuole]
    #20973449 - 12/13/14 11:26 PM (9 years, 1 month ago)

I do 21 plates every time I make them and I used 2 drops of blue the first few times but didn't think it was a deep enough blue so I moved to 3.  I've stuck with 3 since then and it works well.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
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LAGM 2.022 Grow Log


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