|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 8 months
|
|
Quote:
Delection said:
Quote:
FrankHorrigan said: Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly.
I assumed the member was asking if they could use them for no-pour in the PC.
No I should have clarified. I realize these wouldn't survive a PC, I was wondering if anyone has ever attempted using these after media was already PC'd - I am not sure how sterile they would come, but maybe using some antibiotic agar you might get away with it.
seen it at mycotopia. but just poured.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
|
DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
|
|
Thanks for this tek. I'm kind of sick if working with short half pint jars. My only master jar broke last night and I'm devestated that I lost my culture. I had a couple of agar jars with culture from fruit that I threw on WBS so hopefully all is not lost but instead of having 24 jars inoculated I have 5 or 6. I'm slightly devestated but I've got plenty of prints so I just gotta get cracking on agar. However I'm sure to be weeks to a month or more behind now. Love the food coloring idea as well. I'm bout to head to the dollar store. Hopefully they have them there.
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
|
Re: Pasty Agar Tek [Re: DeadPhan]
#19422486 - 01/15/14 11:59 AM (10 years, 14 days ago) |
|
|
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
After using this tek along with a little advice from Cronic about letting my PP5s cool before PCing Im finally starting to have some success with agar. Thanx guys. I was starting to lose my mind a little.
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Pasty Agar Tek [Re: MudaFuka]
#19422697 - 01/15/14 12:52 PM (10 years, 14 days ago) |
|
|
if u end up finding ur mind, put 5g of it in pasty agar recipe and u will see crazy rhino growth!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
|
|
Quote:
blindingleaf said: if u end up finding ur mind, put 5g of it in pasty agar recipe and u will see crazy rhino growth!
Are you saying put five grams of cubes in the agar recipe?
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Pasty Agar Tek [Re: DeadPhan]
#19423061 - 01/15/14 02:11 PM (10 years, 14 days ago) |
|
|
haha no, mudafucker said he was losing his mind, so i said if u find it, put 5 g of ur mind in the recipe. it was a joke...guess not a funny one
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
Quote:
blindingleaf said: haha no, mudafucker said he was losing his mind, so i said if u find it, put 5 g of ur mind in the recipe. it was a joke...guess not a funny one 
I thought it was pretty funny.
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Pasty Agar Tek [Re: MudaFuka]
#19423549 - 01/15/14 03:51 PM (10 years, 14 days ago) |
|
|
cool, thought it went over ppls heads haha.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
|
|
Does this look like a good deal on the mini rounds? They didn't have at dollar store. Decided I check amazon. I'm an amazon prime member. So free shipping.
Link here
--------------------
Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
Edited by DeadPhan (01/15/14 06:20 PM)
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
|
Re: Pasty Agar Tek [Re: DeadPhan]
#19424197 - 01/15/14 06:27 PM (10 years, 14 days ago) |
|
|
Yeah I would say that's good deal
|
Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 9 hours, 17 minutes
|
|
I would say that's a lot of agar dishes. I've been thinking of getting another dozen or so but daaamn.
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Pasty Agar Tek [Re: Generic]
#19427948 - 01/16/14 12:58 PM (10 years, 13 days ago) |
|
|




this is not what it looks like (or maybe it is...)
so this holiday season was crazy for me, let 28 plates go too long. had to toss them and start a new. this is really a testament to not only my laziness (I'm on top of stuff now), and the lack of space in my fridge, but also to Pasty's TEK. these ALL lasted OVER A MONTH AND A HALF with NO refrigeration, survived multiple transfers, dust from smoke, cats, and a dog, and no extra seal, nada. i didn't even keep the unused ones in fridge till use, they all stayed room temp with no extra filter, in a cardboard box. just wanna say thx again pasty, ur tek is awesome, and i wanted to post these here so ppl kno u can almost abuse these things if u make them to a t like the tek, and have little contam (if any, maybe i was lucky...?). In the trash bag pic, thats not a brown contam, thats shiitaki left too long, and browning up a bit in case anyone wondered.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
Violet



Registered: 12/06/11
Posts: 4,205
|
|
This tek's purpose is to be entry-level, yes? It does a good job of that in form and instruction, but I feel it can be trimmed even further.
Yours:
Quote:
You will need: -1 pack of Glad mini rounds -micro pore tape -a drill with a 1/4" bit or very hot nail -paper towels -Foil -PC -Bar of agar agar -Potato flakes -Karo or Honey -Coffee grinder -And of course a PC, even a shitty ass one will work
Mine:
Quote:
You will need: -Ziploc twist-top pint containers -Agar-agar powder -Grainwater from brown rice/grass seed prep, or malt/karo/potato flakes etc. -And of course a PC
Maybe I missed it, but why do you use foil for instance?
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: Pasty Agar Tek [Re: Violet] 1
#19427968 - 01/16/14 01:04 PM (10 years, 13 days ago) |
|
|
pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers. i had asked him this (in PM) kinda about shaving off the amount of foil. i tried with no foil, too much water, but this is what i came up with

instead of wrapping the entire container. works for me, just gotta really press the foil under the lip hard. i just wanted to save foil
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#19427977 - 01/16/14 01:09 PM (10 years, 13 days ago) |
|
|
Quote:
Violet said: This tek's purpose is to be entry-level, yes? It does a good job of that in form and instruction, but I feel it can be trimmed even further.
Yours:
Quote:
You will need: -1 pack of Glad mini rounds -micro pore tape -a drill with a 1/4" bit or very hot nail -paper towels -Foil -PC -Bar of agar agar -Potato flakes -Karo or Honey -Coffee grinder -And of course a PC, even a shitty ass one will work
Mine:
Quote:
You will need: -Ziploc twist-top pint containers -Agar-agar powder -Grainwater from brown rice/grass seed prep, or malt/karo/potato flakes etc. -And of course a PC
Maybe I missed it, but why do you use foil for instance?
the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space. both methods are sound. to each there own
|
Violet



Registered: 12/06/11
Posts: 4,205
|
Re: Pasty Agar Tek [Re: MudaFuka]
#19428061 - 01/16/14 01:26 PM (10 years, 13 days ago) |
|
|
Quote:
MudaFuka said: the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space.
Even "taking up a lot less space" is partially a drawback, because they have so much less grow-out area. I get to an isolate in very few dish transfers because mine have far more area to run out and begin clearer sectoring. And once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8. Additionally, I see that everyone's agar is Very deep in these mini-rounds due to the curvature towards the bottom. They'd have even less area to grow out if agar were used conservatively. However mine have as little as a normal petri dish with the wide area.
I can only see taking up less space as being assuredly a good thing in the case of refrigerator storage as Pasty mentioned. Yet I have had more screw-top dished cultures in the fridge than I even want to, and it hasn't become an issue for me except when I have 20 cultures awaiting the results of elimination testing, in which case fridge storage is still optional...
Quote:
blindingleaf said: pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers.
I'm sure this is the (or at least a) correct answer. It's just a bit of a shame though, needing foil because of needing micropore tape because of needing to modify because of not choosing ready-to-use containers...
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#19428136 - 01/16/14 01:38 PM (10 years, 13 days ago) |
|
|
Quote:
Violet said:
Quote:
MudaFuka said: the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space.
Even "taking up a lot less space" is partially a drawback, because they have so much less grow-out area. I get to an isolate in very few dish transfers because mine have far more area to run out and begin clearer sectoring. And once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8. Additionally, I see that everyone's agar is Very deep in these mini-rounds due to the curvature towards the bottom. They'd have even less area to grow out if agar were used conservatively. However mine have as little as a normal petri dish with the wide area.
I can only see taking up less space as being assuredly a good thing in the case of refrigerator storage as Pasty mentioned. Yet I have had more screw-top dished cultures in the fridge than I even want to, and it hasn't become an issue for me except when I have 20 cultures awaiting the results of elimination testing, in which case fridge storage is still optional...
not every one has a large PC. even with the lid off and the dishes stacked inside one another I can fit fewer screw tops in my PC than mini rounds. Iv found I use much more agar filling screw tops than mini rounds. the extra surface aria is nice and I love the lids. The truth is I prefer the screw tops but I don't understand why you seem to care so much about what other people use.
Edited by MudaFuka (01/16/14 01:40 PM)
|
Violet



Registered: 12/06/11
Posts: 4,205
|
Re: Pasty Agar Tek [Re: MudaFuka]
#19428156 - 01/16/14 01:43 PM (10 years, 13 days ago) |
|
|
Quote:
MudaFuka said: I don't understand why you seem to care so much about what other people use.
Cut that out. I'm just talking about materials and process. I don't "care so much about what other people use" anymore than anyone else does discussing what they do and why.
Quote:
MudaFuka said: not every one has a large PC.
I have an AA910. Very small. I doubt that more than a handful of people buy AAs smaller than 916.
Quote:
MudaFuka said: even with the lid off and the dishes stacked inside one another I can fit fewer screw tops in my PC than mini rounds.
I don't doubt this, I simply don't think it totally makes up for the other factors.
Such as, "once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8." Do you fit Over Double the glad-rounds in the pc?
Personally I don't even use all the dishes I make in my cooker. Some of mine sit in sterile airflow for weeks before I find something to drop in them.
Quote:
MudaFuka said: Iv found I use much more agar filling screw tops than mini rounds.
You use less than ~22mL in the mini rounds?
Even if so, the lesser amount surely doesn't make up for the proportion of lesser area.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
Re: Pasty Agar Tek [Re: Violet]
#19428198 - 01/16/14 01:49 PM (10 years, 13 days ago) |
|
|
sorry I wasn't trying to be rude. You just sounded a bit condescending in your previous replies.
|
|