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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: Munchauzen]
    #20890790 - 11/25/14 08:33 PM (9 years, 2 months ago)

I have tried every primary and secondary colour. I like a mix of green and blue the best.


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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20890797 - 11/25/14 08:35 PM (9 years, 2 months ago)

Quote:

stareatclouds said:

Also Muda's video (which was an LI, not a transfer) definitely showed him holding the plate up and a bit tilted when cutting it out.



It also showed my fingers all over that shit. I really need to remake that thing. I fucking hate it.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20890799 - 11/25/14 08:35 PM (9 years, 2 months ago)

man i need to get some food coloring, still never tried it.

blue and green would probably be my first try.  anything dark seems worthwhile.


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MICROBIAL HUSBANDRY!!!!

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InvisibleMudaFuka
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Re: Pasty Agar Tek [Re: blindingleaf]
    #20890811 - 11/25/14 08:37 PM (9 years, 2 months ago)

:lmafo: My wife went to use some of here food coloring the other day and the only colour left was yellow. She couldn't figure out what the hell happened to it all.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20890830 - 11/25/14 08:41 PM (9 years, 2 months ago)

I could've sworn you posted two videos, one of an LI and one of a regular transfer, or spores to agar, I forget.

I've done blue, green, purple, and black, all pretty dark.

Here's the black ones.



The first one I unfoiled and shook the condensation down for the picture, but the rest look like the second one for the most part.

Tall but, I have a feeling they won't be ineffective at all.  I can maybe even use them for small amounts of grain.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Inocuole]
    #20890835 - 11/25/14 08:43 PM (9 years, 2 months ago)

He posted the LI video and him putting spores on a plate, not transferring to a new plate (but in that one he leaves it flat on the ground, no tilt or anything).


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20890838 - 11/25/14 08:45 PM (9 years, 2 months ago)

Yep I recall that now.  His technique doesn't seem so strange in retrospect.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20891019 - 11/25/14 09:32 PM (9 years, 2 months ago)

Quote:

MudaFuka said:
:lmafo: My wife went to use some of here food coloring the other day and the only colour left was yellow. She couldn't figure out what the hell happened to it all.




That's hilarious :laugh2:


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Offlinehamloaf
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Re: Pasty Agar Tek [Re: MudaFuka]
    #20891862 - 11/26/14 04:52 AM (9 years, 2 months ago)

Quote:

MudaFuka said:
:lmafo: My wife went to use some of here food coloring the other day and the only colour left was yellow. She couldn't figure out what the hell happened to it all.



I am betting you didn't tell her what happened to all the food coloring.


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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: hamloaf]
    #20892454 - 11/26/14 09:36 AM (9 years, 2 months ago)

Anyone, what do you think of using grainwater with coffee(added for the soak)  for agar?


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Fermented Mushrooms!!
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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: pablokabute]
    #20892466 - 11/26/14 09:39 AM (9 years, 2 months ago)

when i used grain water agar, i didn't use it from the actual soak, i would boil 1/2 cup dry grain till they burst, then mash them well with a fork or something, drain that fluid (a little sediment is ok though), then bring it up to 500ml with tap water, and add some malt.  i used coffee in the boiling water too.
IMO grain soak water alone is not nutritious enough to make a good agar mix, but it works none the less.  look into violets TEKs, she is all about that, and covers the topic really well.
i also tried blending grains dry, then adding that to agar mixes, and didn't really like it, but i only attempted it once, and it was WBS, so i can't say for sure.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #20892503 - 11/26/14 09:52 AM (9 years, 2 months ago)

I gotta disagree leaf, grain water has proved to be very nutritious for me. When I make LC with it I dilute it 1:4 and it still supports a huge mass of myc. I use my actual soak water tho, coffee and everything.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20892563 - 11/26/14 10:10 AM (9 years, 2 months ago)

word.  i should try again.  maybe i add to much water to my soaks.  in a 5 gallon bucket, its a little less than half full of grains, and almost to the top with water, so maybe its just more dilute than most :shrug:


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: blindingleaf]
    #20892591 - 11/26/14 10:18 AM (9 years, 2 months ago)

Yeah that may might be it. By the time my grain is hydrated the water is only an inch or so above the grain.


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Offlinespacechildo
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Re: Pasty Agar Tek [Re: blindingleaf]
    #20892617 - 11/26/14 10:26 AM (9 years, 2 months ago)

Quote:

blindingleaf said:
i would boil 1/2 cup dry grain till they burst, then mash them well with a fork or something, drain that fluid (a little sediment is ok though), then bring it up to 500ml with tap water, and add some malt.  i used coffee in the boiling water too.




I do this without the coffee or malt and it still gives me good results.
Actually now a days I've used already prepped grains that's been forgotten and just sat in a jar for a month or 2 instead of fresh grains and boil.
works like a charm, I could even dilute it down more (dont get as much rhizo growth as I want for choosing where to take wedges from.
I dilute about 1:3 or 1:4.


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Offlinehamloaf
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #20892631 - 11/26/14 10:29 AM (9 years, 2 months ago)

Quote:

Pastywhyte said:
I gotta disagree leaf, grain water has proved to be very nutritious for me. When I make LC with it I dilute it 1:4 and it still supports a huge mass of myc. I use my actual soak water tho, coffee and everything.



That's cool.  I have always figured that the grain soak water at the end of the soaking cycle has to be nutritious enough to use as like a plant fertilizer, but never did because I wasn't sure of the NPK,or CEC.

I had never thought of putting grain soak water in my culturing mediums such as agar or LC let alone at how much to begin with.  I am going to have to try this. 

PS- you asked me quite a loaded question in the other thread about LC's, and I just wanted to let you know that I will ham it up on the subject later on when I get a chance to sit down. 

Right now I am kind of busy constructing, building and am about to go in the lab.  After that, it's off to gather field aged horse manure from a few towns over.  There is a horse training facility there.


Edited by liquidmyce (11/26/14 10:37 AM)


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: hamloaf]
    #20892750 - 11/26/14 11:00 AM (9 years, 2 months ago)

I await what I hope will be a most enlightening clairifycation :thumbup:


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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: blindingleaf]
    #20893170 - 11/26/14 01:27 PM (9 years, 2 months ago)

Quote:

blindingleaf said:
when i used grain water agar, i didn't use it from the actual soak, i would boil 1/2 cup dry grain till they burst, then mash them well with a fork or something, drain that fluid (a little sediment is ok though), then bring it up to 500ml with tap water, and add some malt.  i used coffee in the boiling water too.
IMO grain soak water alone is not nutritious enough to make a good agar mix, but it works none the less.  look into violets TEKs, she is all about that, and covers the topic really well.
i also tried blending grains dry, then adding that to agar mixes, and didn't really like it, but i only attempted it once, and it was WBS, so i can't say for sure.





Ive learned grainwater utilization from violets... :smile:

Nice discussion here.


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: β€œAll CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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OfflineHierophant
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Re: Pasty Agar Tek [Re: pablokabute]
    #20896018 - 11/27/14 08:01 AM (9 years, 2 months ago)

I have a cluster that I want to collect a tissue sample from BUT I need to harvest it now and won't be able to make my agar mini rounds until later this evening.

Could I harvest the cluster and keep it in the fridge until the rounds have cooled and do the transfer then?

This is my first time working with agar, thanks for the tek Pasty


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Re: Pasty Agar Tek [Re: Hierophant]
    #20896021 - 11/27/14 08:02 AM (9 years, 2 months ago)

Quote:

Hierophant said:
Could I harvest the cluster and keep it in the fridge until the rounds have cooled and do the transfer then?




You sure can :thumbup:


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