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magickspore
Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 10 months, 1 day
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Quote:
Delection said: You might want to check these out Pasty.
I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene). This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.
I have run a test batch and they did hold up at 15psi for 15 min. I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!).
Here are some pics below.
Packaging
Size comparison
Measurements
Polypropelene stamp
120 of them neatly packed away in a 30x20x10cm little plastic bin.
Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?
Finally starting agar today
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cronicr
Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 2 months
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my contaminated recycled agar is looking great
-------------------- It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Delection
Spiritual Vagabond
Registered: 10/08/11
Posts: 848
Loc:
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Quote:
magickspore said:
Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?
Finally starting agar today
Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.
Has anyone tried agar work with those little restaurant containers similar to the ones below?
They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.
-------------------- Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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we have those to where i work, but they are PP2...are those PP5? if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Quote:
blindingleaf said: we have those to where i work, but they are PP2...are those PP5? if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.
The water and steam are the same temp.
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blindingleaf
blue collar underworld
Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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haha yea i guess ur right. but i thought with the pasty tek we have to raise them a bit above the water line? i always do anyways...i guess to prevent over boiling?
edit: just re read tek, I'm an idiot no mention of raising above water level. guess I'm paranoid too haha
Edited by blindingleaf (01/06/14 05:06 PM)
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Alwaysanoob1
Old school
Registered: 12/14/13
Posts: 11
Loc: Mid west
Last seen: 10 years, 2 hours
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Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think
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magickspore
Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 10 months, 1 day
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Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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Quote:
blindingleaf said: haha yea i guess ur right. but i thought with the pasty tek we have to raise them a bit above the water line? i always do anyways...i guess to prevent over boiling?
edit: just re read tek, I'm an idiot no mention of raising above water level. guess I'm paranoid too haha
You should raise the container above the water, guess I took it for granted that people would do that. I will edit the OP.
Quote:
Alwaysanoob1 said: Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think
Glad your on the agar wagon A little condensation won't hurt nothing and will usually clear up in a day or two
Quote:
magickspore said: Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.
It should be like jello
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magickspore
Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 10 months, 1 day
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Quote:
Pastywhyte said:
Quote:
magickspore said: Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.
It should be like jello
lol ok that's what I thought I must have done something wrong. Because it was like dry mashed taters .
Other than that everything seems ok. I let them reach room temp, loaded PC, PCd for about 45-50 min (I did that because my regulator was only holding 11-13 psi. I'll have to look into that.) Now its cooling got about an 1/8th to 1/4" on the bottom of my containers. Once they're done cooling, time to have some fun.
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J. Jack Flash
stranger than ever.
Registered: 11/20/13
Posts: 1,500
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Quote:
Delection said:
Quote:
magickspore said:
Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?
Finally starting agar today
Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.
Has anyone tried agar work with those little restaurant containers similar to the ones below?
They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.
cool idea, tho i strongly doubt jello shot cups will hold up in the pressure cooker.
-------------------- the j stands for jesus.2020 new years grow along
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Those will melt to shit. I wouldn't waste my time.
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mushrume man
Sadis Factory
Registered: 11/18/08
Posts: 375
Loc: PNW
Last seen: 2 years, 2 months
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People use them, but straight from the sleeve they come in, and only if it is unpunctured sleeve. Just like disposable petri dishes
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FrankHorrigan
The Inquisition
Registered: 01/04/11
Posts: 10,573
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Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly.
I assumed the member was asking if they could use them for no-pour in the PC.
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Delection
Spiritual Vagabond
Registered: 10/08/11
Posts: 848
Loc:
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Quote:
FrankHorrigan said: Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly.
I assumed the member was asking if they could use them for no-pour in the PC.
No I should have clarified. I realize these wouldn't survive a PC, I was wondering if anyone has ever attempted using these after media was already PC'd - I am not sure how sterile they would come, but maybe using some antibiotic agar you might get away with it.
-------------------- Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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Psilicon
Really Nice Guy
Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
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Re: Pasty Agar Tek [Re: Delection] 1
#19384215 - 01/07/14 06:25 PM (10 years, 2 months ago) |
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Antiobiotic agar won't protect you from mold spores, sadly.
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Delection
Spiritual Vagabond
Registered: 10/08/11
Posts: 848
Loc:
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Re: Pasty Agar Tek [Re: Psilicon]
#19388739 - 01/08/14 03:31 PM (10 years, 2 months ago) |
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Quote:
van der griegen said: Antiobiotic agar won't protect you from mold spores, sadly.
I know gentamicin does not kill mold spores, thats stating the obvious.. I guess I am going to have to try them and find out myself.
-------------------- Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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When I get home I will take a pic of tetracycline agar covered in mold for ya
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Galaxy
Registered: 06/03/13
Posts: 61
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Okay, so the agar should be thick and jelly like, correct? I've made one missed attempt by using a watery agar, so im guessing this is a waste and I should start again.. I guess I was a little to baked when making the agar.
What tek would you go too from here, for adding spores to the mix? I had a half of a syringe and several spore prints from my previous grow. I've read several teks on how to go about this process, but being this is your tek I would like to know what your preffered method is.
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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Re: Pasty Agar Tek [Re: Galaxy]
#19394001 - 01/09/14 02:42 PM (10 years, 2 months ago) |
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To inoculate with ms solution I like to first heat my loop red hot and then use it to melt a little divot into the surface of the agar. Then use a single drop right into the divot. This will help keep it from sliding around the plate. Leave it in the SAB for a day or two which will absorb the water into the agar, leaving the spores in one spot. This helps keep any bacteria confined and will make it easier to transfer away from.
With a print, flame your loop or scalpel, then cool it in the agar. Scrape up a tiny amount of spores, then drag the loop or tip of scalpel across the surface. You could also hold the print above the plate and flick the spores onto the plate. This can be a little risky for introducing contams but does allow you to identify faster genetics. Ultimately you will need to experiment a bit and decide what works for you
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