Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemagickspore
Male User Gallery


Registered: 12/11/12
Posts: 798
Loc: Center of the universe. Flag
Last seen: 10 months, 1 day
Re: Pasty Agar Tek [Re: Delection]
    #19377278 - 01/06/14 01:37 PM (10 years, 2 months ago)

Quote:

Delection said:
You might want to check these out Pasty.

I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene).  This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.

I have run a test batch and they did hold up at 15psi for 15 min.  I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!). 

Here are some pics below.

Packaging



Size comparison



Measurements





Polypropelene stamp



120 of them neatly packed away in a 30x20x10cm little plastic bin.






Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 2 months
Trusted Cultivator
Re: Pasty Agar Tek [Re: magickspore]
    #19377408 - 01/06/14 02:10 PM (10 years, 2 months ago)

my contaminated recycled agar is looking great:homerdoh3:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
InvisibleDelection
Spiritual Vagabond
 User Gallery

Registered: 10/08/11
Posts: 848
Loc: Flag
Re: Pasty Agar Tek [Re: magickspore]
    #19377653 - 01/06/14 03:08 PM (10 years, 2 months ago)

Quote:

magickspore said:

Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:




Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.

Has anyone tried agar work with those little restaurant containers similar to the ones below?





They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.


--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Pasty Agar Tek [Re: Delection]
    #19377776 - 01/06/14 03:30 PM (10 years, 2 months ago)

we have those to where i work, but they are PP2...are those PP5?
if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: blindingleaf]
    #19378105 - 01/06/14 04:58 PM (10 years, 2 months ago)

Quote:

blindingleaf said:
we have those to where i work, but they are PP2...are those PP5?
if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.




:lolwut:

The water and steam are the same temp.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19378120 - 01/06/14 05:01 PM (10 years, 2 months ago)

haha yea i guess ur right.  but i thought with the pasty tek we have to raise them a bit above the water line?  i always do anyways...i guess to prevent over boiling?

edit:  just re read tek, I'm an idiot :shrug:  no mention of raising above water level.  guess I'm paranoid too haha

Edited by blindingleaf (01/06/14 05:06 PM)

Extras: Filter Print Post Top
OfflineAlwaysanoob1
Old school
Male User Gallery

Registered: 12/14/13
Posts: 11
Loc: Mid west
Last seen: 10 years, 2 hours
Re: Pasty Agar Tek [Re: blindingleaf]
    #19378137 - 01/06/14 05:05 PM (10 years, 2 months ago)

Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think

Extras: Filter Print Post Top
Offlinemagickspore
Male User Gallery


Registered: 12/11/12
Posts: 798
Loc: Center of the universe. Flag
Last seen: 10 months, 1 day
Re: Pasty Agar Tek [Re: Alwaysanoob1]
    #19378207 - 01/06/14 05:24 PM (10 years, 2 months ago)

Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,830
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: magickspore]
    #19378890 - 01/06/14 07:24 PM (10 years, 2 months ago)

Quote:

blindingleaf said:
haha yea i guess ur right.  but i thought with the pasty tek we have to raise them a bit above the water line?  i always do anyways...i guess to prevent over boiling?

edit:  just re read tek, I'm an idiot :shrug:  no mention of raising above water level.  guess I'm paranoid too haha




You should raise the container above the water, guess I took it for granted that people would do that.  I will edit the OP.


Quote:

Alwaysanoob1 said:
Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think




Glad your on the agar wagon :rockon: A little condensation won't hurt nothing and will usually clear up in a day or two :super:


Quote:

magickspore said:
Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.




It should be like jello :lol:

Extras: Filter Print Post Top
Offlinemagickspore
Male User Gallery


Registered: 12/11/12
Posts: 798
Loc: Center of the universe. Flag
Last seen: 10 months, 1 day
Re: Pasty Agar Tek [Re: Pastywhyte]
    #19379023 - 01/06/14 07:49 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

magickspore said:
Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.




It should be like jello :lol:




lol ok that's what I thought I must have done something wrong. Because it was like dry mashed taters :rofl:.

Other than that everything seems ok. I let them reach room temp, loaded PC, PCd for about 45-50 min (I did that because my regulator was only holding 11-13 psi. I'll have to look into that.) Now its cooling got about an 1/8th to 1/4" on the bottom of my containers. Once they're done cooling, time to have some fun.

Extras: Filter Print Post Top
InvisibleJ. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
Re: Pasty Agar Tek [Re: Delection]
    #19379283 - 01/06/14 08:31 PM (10 years, 2 months ago)

Quote:

Delection said:
Quote:

magickspore said:

Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:




Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.

Has anyone tried agar work with those little restaurant containers similar to the ones below?





They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.




cool idea, tho i strongly doubt jello shot cups will hold up in the pressure cooker.


--------------------
 
the j stands for jesus.
2020 new years grow along

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: J. Jack Flash] * 1
    #19379381 - 01/06/14 08:45 PM (10 years, 2 months ago)

Those will melt to shit. I wouldn't waste my time.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
Offlinemushrume man
Sadis Factory
Male User Gallery


Registered: 11/18/08
Posts: 375
Loc: PNW
Last seen: 2 years, 2 months
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19379620 - 01/06/14 09:43 PM (10 years, 2 months ago)

People use them, but straight from the sleeve they come in, and only if it is unpunctured sleeve. Just like disposable petri dishes


--------------------

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: mushrume man]
    #19379661 - 01/06/14 09:51 PM (10 years, 2 months ago)

Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly. 

I assumed the member was asking if they could use them for no-pour in the PC.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
InvisibleDelection
Spiritual Vagabond
 User Gallery

Registered: 10/08/11
Posts: 848
Loc: Flag
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19380604 - 01/07/14 01:44 AM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly. 

I assumed the member was asking if they could use them for no-pour in the PC.




No I should have clarified.  I realize these wouldn't survive a PC, I was wondering if anyone has ever attempted using these after media was already PC'd - I am not sure how sterile they would come, but maybe using some antibiotic agar you might get away with it.


--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
Re: Pasty Agar Tek [Re: Delection] * 1
    #19384215 - 01/07/14 06:25 PM (10 years, 2 months ago)

Antiobiotic agar won't protect you from mold spores, sadly.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

Extras: Filter Print Post Top
InvisibleDelection
Spiritual Vagabond
 User Gallery

Registered: 10/08/11
Posts: 848
Loc: Flag
Re: Pasty Agar Tek [Re: Psilicon]
    #19388739 - 01/08/14 03:31 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Antiobiotic agar won't protect you from mold spores, sadly.




I know gentamicin does not kill mold spores, thats stating the obvious..  I guess I am going to have to try them and find out myself.


--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,830
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: Delection]
    #19388791 - 01/08/14 03:40 PM (10 years, 2 months ago)

When I get home I will take a pic of tetracycline agar covered in mold for ya :wink:

Extras: Filter Print Post Top
InvisibleGalaxy


Registered: 06/03/13
Posts: 61
Re: Pasty Agar Tek [Re: Pastywhyte]
    #19393759 - 01/09/14 01:41 PM (10 years, 2 months ago)

Okay, so the agar should be thick and jelly like, correct? I've made one missed attempt by using a watery agar, so im guessing this is a waste and I should start again.. I guess I was a little to baked when making the agar.

What tek would you go too from here, for adding spores to the mix? I had a half of a syringe and several spore prints from my previous grow. I've read several teks on how to go about this process, but being this is your tek I would like to know what your preffered method is.

Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,830
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: Galaxy]
    #19394001 - 01/09/14 02:42 PM (10 years, 2 months ago)

To inoculate with ms solution I like to first heat my loop red hot and then use it to melt a little divot into the surface of the agar. Then use a single drop right into the divot. This will help keep it from sliding around the plate. Leave it in the SAB for a day or two which will absorb the water into the agar, leaving the spores in one spot. This helps keep any bacteria confined and will make it easier to transfer away from.

With a print, flame your loop or scalpel, then cool it in the agar. Scrape up a tiny amount of spores,  then drag the loop or tip of scalpel across the surface. You could also hold the print above the plate and flick the spores onto the plate. This can be a little  risky for introducing contams but does allow you to identify faster genetics. Ultimately you will need to experiment a bit and decide what works for you :super:

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,769 8 12/13/19 06:16 PM
by mushboy
* Agar Tek Aristar 5,849 12 08/01/03 06:42 PM
by shirley knott
* Easy syringe tek? pleasesmokemarijuana 2,371 8 04/02/03 07:09 PM
by Roadkill
* Easy Poo Tek Audi0 3,440 8 12/15/02 09:04 AM
by PDU
* Re: Peroxidated Agar Tek sparks 4,582 6 05/08/00 12:43 AM
by Anonymous
* easy agar source? KafkaPie 1,095 1 05/13/03 11:43 PM
by micro
* Easy Syringe Tek? Tenchi 2,799 18 01/28/03 05:06 AM
by Azmodeus
* agar tek? the man 2,780 4 12/16/02 10:45 AM
by the man

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
800,854 topic views. 30 members, 183 guests and 84 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.01 seconds on 15 queries.