Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Grow Bags   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: < First | < Back | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Munchauzen]
    #20868763 - 11/21/14 01:42 AM (9 years, 2 months ago)

I only got these syringes so I could get a grow out of them and then make prints out of the best fruits so I can continue playing with agar for a long time to come.  Something I should've done when I started but at least now I appreciate what I'm saving myself from.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Pasty Agar Tek [Re: Inocuole]
    #20868764 - 11/21/14 01:44 AM (9 years, 2 months ago)

Quote:

Inocuole said:
Quote:

Munchauzen said:
I got like a dozen colonization spots or so.




That sounds really difficult to know where you want to transfer from.




So, I was just watching LGM, and RR says to just transfer all growth, and within a week any contams will sporulate and you can just toss them.


Extras: Filter Print Post Top
Offlinespirit_shadow
Feature not a bug
I'm a teapot User Gallery


Registered: 08/15/11
Posts: 25,672
Last seen: 1 hour, 35 minutes
Re: Pasty Agar Tek [Re: Munchauzen]
    #20868775 - 11/21/14 01:50 AM (9 years, 2 months ago)

how would one transfer away from contams? Do you have to catch the contsm as soon as you notice it then take a piece of good myc to another plate?


--------------------
ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011
Ban lotto


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: spirit_shadow]
    #20868785 - 11/21/14 01:55 AM (9 years, 2 months ago)

Not really, the contam can only travel in 2 dimensions so if it's far enough away from the healthy growth then you can just use a tool to grab the tinest piece of clean mycelium that you can and set it down on a new plate.  It's a good idea to be on the ball about it but there isn't necessarily a rush, depending on how bad the plate is.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: Inocuole]
    #20868796 - 11/21/14 02:06 AM (9 years, 2 months ago)

Quote:

Inocuole said:
Cool, my only concern now is that there may be too much moisture in that plate.



What I've noticed will happen in that situation is over the course of around a week or two the water that's sitting on top of the agar will slowly absorb agar up into it and kind of gel up, so if it's a single drop it'll look kind of like a nipple raised up out of the agar.  Spores will then germinate on top of the drop or pool, or if it's LC the myc will grow in a cloud within the water and then force its way out.  Sometimes the liquid will absorb into the agar though, really just depends on how moist the agar was to begin with.  Squirting a drop onto an inoculation loop and then rubbing the loop on the agar works well to avoid that situation.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20868798 - 11/21/14 02:10 AM (9 years, 2 months ago)

Good idea, I never thought about that.  I'm using this as my "loop" so I don't know how well it would actually work:



--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: Inocuole]
    #20868819 - 11/21/14 02:42 AM (9 years, 2 months ago)

Shouldn't be an issue.  Just so long as you coat the wire with liquid it should be covered in spores so just touch it to the agar and wipe it in a short line.  I use the metal ripped out of a bread loaf tie taped to a pencil and it works fine.  Yours looks a lot more professional than mine lol.  If you were to bend the loop at an angle to your handle you could probably touch more of the wire to the agar than just the tip but it's not really necessary.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20868877 - 11/21/14 04:06 AM (9 years, 2 months ago)

Pretty sure just turning it sideways would make a good line.  It's just a .16 or so guitar string.  Pretty sure it's the string for G, so it's almost paper clip thick, but it has really good tension now and feels like it's made of something stronger than it is.

I am worried about it becoming brittle though.  I suppose agar would never give it enough resistance to break but alas.

The bread tie thing is a good idea, I have a ton of those laying around.  I may make a few different tools for different applications.  Or, rather than tools, just make different attachments for the tip of the x-acto that I'm using.

Speaking of which, I was using a lighter before I got a torch and the torch actually vaporizes the soot that the lighter produced originally, and now some of my other tools look cleaner than they did before. :lol:


So that this isn't just me rambling on, if you're thinking of getting into this tek and haven't yet, I HIGHLY recommend an x-acto knife to use as a base for different tips(like the blade it comes with, the loop thing, or one that's just a hooked needle for stabbing), and a torch of some kind.  The culinary torch I got off amazon for less than 10 dollars works great.  You want things to glow orange as quickly as possible and torches do that and more. (More being, if you're into dabs :wink: )


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleMudaFuka
Poppin bottles
Male User Gallery


Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: Inocuole]
    #20868991 - 11/21/14 05:58 AM (9 years, 2 months ago)

Using too many spores on agar makes growth verry hard to track. Making it tough to spot some molds. As long as they are all in one spot on the plate it might not be such a big deal though. I don't always get around to transfering away from contams right away but it is a good idea to do it before mold has a chance to sporulate. I made my loop out of a guitar string. It works great.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: MudaFuka]
    #20869001 - 11/21/14 06:06 AM (9 years, 2 months ago)

My loop is the nichrome element from one of the dehydrators I burnt out, then just put in an exacto handel. Before that I just used a straightened out paper clip. Many things can work :super:


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Pastywhyte]
    #20869020 - 11/21/14 06:15 AM (9 years, 2 months ago)

Are there any other useful tool shapes that are good for certain things?

I can only think of loop, blade, and needle as being effective.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: Inocuole]
    #20869049 - 11/21/14 06:24 AM (9 years, 2 months ago)

I have a dental tool for cleaning dogs teeth that works good. Its amazing for reaching down a slant and hooking out a bunch of the agar.


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Pasty Agar Tek [Re: Pastywhyte]
    #20869086 - 11/21/14 06:38 AM (9 years, 2 months ago)

yea, slant tools are important.  long, thin, and curved at the end is ideal.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Pasty Agar Tek [Re: blindingleaf]
    #20869110 - 11/21/14 06:49 AM (9 years, 2 months ago)



Edited by Munchauzen (11/21/14 06:50 AM)


Extras: Filter Print Post Top
OfflinePsyCLown89
Shroomaloomed
 User Gallery


Registered: 08/18/14
Posts: 789
Loc: South Africa Flag
Last seen: 2 years, 1 month
Re: Pasty Agar Tek [Re: Munchauzen]
    #20869142 - 11/21/14 07:09 AM (9 years, 2 months ago)

What are potato flakes?

I did a quick google and not much turned up for South Africa (where I stay) except for smash / instant mash... Could I possibly use instant mash as opposed to potato flakes?


--------------------
My Trade List - Click ME!!   [Updated: 02/05/2015]




"It's all in your head"
"Haste makes waste!"


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: PsyCLown89]
    #20869146 - 11/21/14 07:10 AM (9 years, 2 months ago)

Potato flakes are instant mashed potatoes :shrug:


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: Pastywhyte]
    #20869190 - 11/21/14 07:28 AM (9 years, 2 months ago)

Gonna trip acid this week and start my first Golden Teacher agar project next week, grabbing your glad mini-rounds also since they seem like a better option then using my big 'ole widemouths. :headbanger:

Just to make sure I have everything right:
I'm doing this from MS syringe so I'd flame the needle and then release a DROP of spore onto agar? I've been reading that that's all it takes. Now for transferring from the glad mini's, do I just open in SAB, cut out some Myc and place it in my grain jar or transfer the whole agar? From what I've seen with petri dishes people just take the whole piece of agar and drop that in the grains, can I do that with these?

Also, (And this one is a noob question), do I have to wait for full colonization of the agar before transfering it to grain?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Pastywhyte]
    #20869203 - 11/21/14 07:31 AM (9 years, 2 months ago)

After you flame the syringe, but before you inoculate, let a drop or two fall out the syringe to cool it so that it doesn't kill that one drop that you would otherwise inoculate with.

Also no you can inoculate with a half-colonized piece of agar and it should take over as long as your procedure is good.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Pasty Agar Tek [Re: Munchauzen]
    #20869204 - 11/21/14 07:31 AM (9 years, 2 months ago)

Quote:

Munchauzen said:
http://www.amazon.com/HTS-156W1-Stainless-Steel-Carver/dp/B000BGL5ZC/ref=sr_1_8?s=hpc&ie=UTF8&qid=1416577708&sr=1-8&keywords=dental+tools





yea!!  those look nice!!  i try for at least 7" long for slants, because i like to flame pretty high up the stem because its going far into the tube sometimes, and u don't wanna be holding a scolding hot piece of metal.  on that amazon link there should be a tool for ~$4 that is 9" long, with a spoon on one end and a spatula on the other, thats the one i use.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Pasty Agar Tek [Re: blindingleaf]
    #20869212 - 11/21/14 07:33 AM (9 years, 2 months ago)



--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | Next > | Last >

Shop: Mushroom-Hut Grow Bags   North Spore Injection Grain Bag   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,732 8 12/13/19 06:16 PM
by mushboy
* Agar Tek Aristar 5,815 12 08/01/03 06:42 PM
by shirley knott
* Easy syringe tek? pleasesmokemarijuana 2,357 8 04/02/03 07:09 PM
by Roadkill
* Easy Poo Tek Audi0 3,429 8 12/15/02 09:04 AM
by PDU
* Re: Peroxidated Agar Tek sparks 4,555 6 05/08/00 12:43 AM
by Anonymous
* easy agar source? KafkaPie 1,092 1 05/13/03 11:43 PM
by micro
* Easy Syringe Tek? Tenchi 2,789 18 01/28/03 05:06 AM
by Azmodeus
* agar tek? the man 2,773 4 12/16/02 10:45 AM
by the man

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
794,002 topic views. 32 members, 282 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.01 seconds on 13 queries.