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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I only got these syringes so I could get a grow out of them and then make prints out of the best fruits so I can continue playing with agar for a long time to come. Something I should've done when I started but at least now I appreciate what I'm saving myself from.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Pasty Agar Tek [Re: Inocuole]
#20868764 - 11/21/14 01:44 AM (9 years, 2 months ago) |
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Quote:
Inocuole said:
Quote:
Munchauzen said: I got like a dozen colonization spots or so.
That sounds really difficult to know where you want to transfer from.
So, I was just watching LGM, and RR says to just transfer all growth, and within a week any contams will sporulate and you can just toss them.
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spirit_shadow
Feature not a bug



Registered: 08/15/11
Posts: 25,672
Last seen: 1 hour, 35 minutes
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how would one transfer away from contams? Do you have to catch the contsm as soon as you notice it then take a piece of good myc to another plate?
-------------------- ERROR 418 IM A TEAPOT.....(this account is automated, all posts related to illegal activities or advice thereof are strictly from numerous online sites and are for informational purposes only)- Circa 2011 Ban lotto
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Not really, the contam can only travel in 2 dimensions so if it's far enough away from the healthy growth then you can just use a tool to grab the tinest piece of clean mycelium that you can and set it down on a new plate. It's a good idea to be on the ball about it but there isn't necessarily a rush, depending on how bad the plate is.
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Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
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Re: Pasty Agar Tek [Re: Inocuole]
#20868796 - 11/21/14 02:06 AM (9 years, 2 months ago) |
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Quote:
Inocuole said: Cool, my only concern now is that there may be too much moisture in that plate.
What I've noticed will happen in that situation is over the course of around a week or two the water that's sitting on top of the agar will slowly absorb agar up into it and kind of gel up, so if it's a single drop it'll look kind of like a nipple raised up out of the agar. Spores will then germinate on top of the drop or pool, or if it's LC the myc will grow in a cloud within the water and then force its way out. Sometimes the liquid will absorb into the agar though, really just depends on how moist the agar was to begin with. Squirting a drop onto an inoculation loop and then rubbing the loop on the agar works well to avoid that situation.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Good idea, I never thought about that. I'm using this as my "loop" so I don't know how well it would actually work:
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Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
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Re: Pasty Agar Tek [Re: Inocuole]
#20868819 - 11/21/14 02:42 AM (9 years, 2 months ago) |
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Shouldn't be an issue. Just so long as you coat the wire with liquid it should be covered in spores so just touch it to the agar and wipe it in a short line. I use the metal ripped out of a bread loaf tie taped to a pencil and it works fine. Yours looks a lot more professional than mine lol. If you were to bend the loop at an angle to your handle you could probably touch more of the wire to the agar than just the tip but it's not really necessary.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Pretty sure just turning it sideways would make a good line. It's just a .16 or so guitar string. Pretty sure it's the string for G, so it's almost paper clip thick, but it has really good tension now and feels like it's made of something stronger than it is.
I am worried about it becoming brittle though. I suppose agar would never give it enough resistance to break but alas.
The bread tie thing is a good idea, I have a ton of those laying around. I may make a few different tools for different applications. Or, rather than tools, just make different attachments for the tip of the x-acto that I'm using.
Speaking of which, I was using a lighter before I got a torch and the torch actually vaporizes the soot that the lighter produced originally, and now some of my other tools look cleaner than they did before. 
So that this isn't just me rambling on, if you're thinking of getting into this tek and haven't yet, I HIGHLY recommend an x-acto knife to use as a base for different tips(like the blade it comes with, the loop thing, or one that's just a hooked needle for stabbing), and a torch of some kind. The culinary torch I got off amazon for less than 10 dollars works great. You want things to glow orange as quickly as possible and torches do that and more. (More being, if you're into dabs )
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20868991 - 11/21/14 05:58 AM (9 years, 2 months ago) |
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Using too many spores on agar makes growth verry hard to track. Making it tough to spot some molds. As long as they are all in one spot on the plate it might not be such a big deal though. I don't always get around to transfering away from contams right away but it is a good idea to do it before mold has a chance to sporulate. I made my loop out of a guitar string. It works great.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: MudaFuka]
#20869001 - 11/21/14 06:06 AM (9 years, 2 months ago) |
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My loop is the nichrome element from one of the dehydrators I burnt out, then just put in an exacto handel. Before that I just used a straightened out paper clip. Many things can work
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Are there any other useful tool shapes that are good for certain things?
I can only think of loop, blade, and needle as being effective.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20869049 - 11/21/14 06:24 AM (9 years, 2 months ago) |
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I have a dental tool for cleaning dogs teeth that works good. Its amazing for reaching down a slant and hooking out a bunch of the agar.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea, slant tools are important. long, thin, and curved at the end is ideal.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Edited by Munchauzen (11/21/14 06:50 AM)
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PsyCLown89
Shroomaloomed



Registered: 08/18/14
Posts: 789
Loc: South Africa
Last seen: 2 years, 1 month
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What are potato flakes?
I did a quick google and not much turned up for South Africa (where I stay) except for smash / instant mash... Could I possibly use instant mash as opposed to potato flakes?
-------------------- My Trade List - Click ME!! [Updated: 02/05/2015]
"It's all in your head" "Haste makes waste!"
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Potato flakes are instant mashed potatoes
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Gonna trip acid this week and start my first Golden Teacher agar project next week, grabbing your glad mini-rounds also since they seem like a better option then using my big 'ole widemouths. 
Just to make sure I have everything right: I'm doing this from MS syringe so I'd flame the needle and then release a DROP of spore onto agar? I've been reading that that's all it takes. Now for transferring from the glad mini's, do I just open in SAB, cut out some Myc and place it in my grain jar or transfer the whole agar? From what I've seen with petri dishes people just take the whole piece of agar and drop that in the grains, can I do that with these?
Also, (And this one is a noob question), do I have to wait for full colonization of the agar before transfering it to grain?
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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After you flame the syringe, but before you inoculate, let a drop or two fall out the syringe to cool it so that it doesn't kill that one drop that you would otherwise inoculate with.
Also no you can inoculate with a half-colonized piece of agar and it should take over as long as your procedure is good.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
Munchauzen said: http://www.amazon.com/HTS-156W1-Stainless-Steel-Carver/dp/B000BGL5ZC/ref=sr_1_8?s=hpc&ie=UTF8&qid=1416577708&sr=1-8&keywords=dental+tools

yea!! those look nice!! i try for at least 7" long for slants, because i like to flame pretty high up the stem because its going far into the tube sometimes, and u don't wanna be holding a scolding hot piece of metal. on that amazon link there should be a tool for ~$4 that is 9" long, with a spoon on one end and a spatula on the other, thats the one i use.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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