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Munchauzen



Registered: 06/22/11
Posts: 14,342
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looks like I have my weekend plans set

I did change the tek a little, though. I will be ingesting 12 flavor gummibears during the procedure. I expect to see improved results.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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That satellite in the second pic looks like mold. The mycelium growing from the center looks fine though. Make some transfers from the leading edge.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: MudaFuka]
#20810051 - 11/08/14 01:45 PM (9 years, 2 months ago) |
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Damn, I thought it was a nice, white chunk of mycelium. Thanks, y'all. I'll transfer that bitch tomorrow or the next day.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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It is white myc, I would say trich.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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any time you see circular white growth on the edge of a plate like that you can bet it's mold.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: MudaFuka]
#20810376 - 11/08/14 03:25 PM (9 years, 2 months ago) |
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I always figured trich was explicitly green. Thanks to both of you. A few questions, if you're feeling up to it.
1) Given that the whole chunk is so close to the contaminated portion, would simply using the "good" mycelium would be too risky without transferring first to observe? Or do y'all sometimes just isolate it right then and toss it into a jar or w/e.
2) Typically, how many pieces of the edges you guys take to transfer?
3) When I transferred my first pieces of agar into a new plate, the chunk was pretty big. Like, imagine an iceberg where only the tip is showing. Well the tip was mycelium, 90% was uncolonized agar. Do y'all try and get the pieces as tiny as possible?
4) My mycelium always looks super weak and sporadic, e.g., no rhizomorphic qualities, very thin, etc. Is this normal with cubensis strains or am I doing something wrong?
Thanks!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Rizos don't really mean anything. they just look good. When I transfer I take a piece about the size of a grain of rice. I would take a smaller piece if I could. Generally when I'm trying to Clean a culture I will take a few transfers just to increase my odds of getting a clean one. The colour of mold is actually just its spores. The mold mycelium is generally white or gray.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: MudaFuka]
#20810634 - 11/08/14 04:58 PM (9 years, 2 months ago) |
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Thanks man, that's helpful. When you take multiple transfers in a single dish (not split into 3 or whatever) like a PP, do you use multiple PPs or put 3 rice-size transfers into a triangle?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I use a different dish for each transfer.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: MudaFuka]
#20811725 - 11/08/14 09:30 PM (9 years, 2 months ago) |
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I asked this in Muda's thread with his videos but I want the consensus in here as well, do you pick up the plates when you do your transfers, or just lower your hand to the floor of the SAB and hold the plate still? Picking it up, now that I've considered it, seems like it could fuck up the whole point of the SAB.
Thoughts?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: Inocuole]
#20811788 - 11/08/14 09:47 PM (9 years, 2 months ago) |
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Even if it's on the ground and still, you're still opening the lid and moving it away, aren't you? Wouldn't that be the same issue as picking up the whole plate and holding it in air?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Well I dunno. I can't tell how much stuff is simply suspended in the air.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: Inocuole]
#20811844 - 11/08/14 10:00 PM (9 years, 2 months ago) |
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I didn't think anything suspended in air by the time you use the SAB. It just floated about if you disrupt the still air, which opening a lid and moving it would just like holding the plate up?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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So what's the consensus on this one? It looks like it all just ran towards one side of the plate and not in all directions. I snapped a few regular pics in SAB, a magnified one and a closeup of the side.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
stareatclouds said: I didn't think anything suspended in air by the time you use the SAB. It just floated about if you disrupt the still air, which opening a lid and moving it would just like holding the plate up?
Ideally it would be dragged down and stick to the wet floor or walls of the sab. When you bring your tools in from flaming or make jerky movements in the SAB there is the possibility of spores being brought in and stirred up. Waiting after movement like that will let them settle and get stuck again.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Right, so in theory, wouldn't lifting up a plate be the same as taking off a lid and moving it? They both seem to require the same amount of movement.
I wonder if anyone has ran any actual tests on this. Like placing stuff in there after x amount of minutes or at different places in a SAB and seeing how much stuff is still around, optimal amount of wait time, etc.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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everytime u do agar in an SAB, its kind of like a test, and eventually u form ur own procedure based on success rate.
after i pour my agar, i leave the bottle in the SAB for a few days, no lid on bottle. it will take about 5-6 days for mold to germinate, even with no movement in my SAB. air currents in my house, 3 pets, and a smoker contribute to this though. other people I'm sure have way cleaner living conditions than i do.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I had some slants boil over a couple months back (water level too low) so I left em in the jar with the grain lid still on. Took a few months but a penicillin spore eventually worked through the filter and contamed the hell outta it. Know your environment and take steps to protect your media from excessive exposure and spore loads. Moral is that no filter is perfect and no ones house is really clean.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Since I have moved my SAB into a sealed plastic room with a heap air cleaner running when it's not in use my contams have dropped significantly. I do a lot of rushed sterile work when I'm half asleep and even with some pretty sketch technique my success rate is pretty good.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: MudaFuka]
#20814361 - 11/09/14 01:49 PM (9 years, 2 months ago) |
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I've been thinking about getting a purifier/filter just for that room to run for a while before I turn off all the air.
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