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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19355743 - 01/01/14 06:15 PM (10 years, 28 days ago)

chronin
how firm r u letting it get? id imagine u can get it all the way firm and put it in because its just going to liquify again in PC, but r u letting it firm up to just to the point where that middle agar blob happens?


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #19355762 - 01/01/14 06:19 PM (10 years, 28 days ago)

not really going by how firm it gets just trying to get it to room temp so when you close the lid you don't get condensation build up and everything is starting at the same temp during the pc cycle and cooling at the same temp once you shut the pc off:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19355826 - 01/01/14 06:38 PM (10 years, 28 days ago)

cool sounds good.
can u explain to me the theory of not letting pc totally cool, i.e. pc drops to 0, but is still hot, so when u take weighted gauge off, steam comes out for maybe 10 seconds.  there was/is talk of how that may be introducing con tams via outside air being sucked in while steam is being released thru top vent.  is this true?

edit: i have also read that if u don't let it cool completely, like in above example or say when PC is at 1psi, and u take weight off, air will also be sucked out of jar thru filter as the steam/air in PC RUSHES to the vent to escape. is this true also, and is this a concern for contam or for something else?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Edited by blindingleaf (01/01/14 06:40 PM)


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: blindingleaf]
    #19355860 - 01/01/14 06:49 PM (10 years, 28 days ago)

Quote:

blindingleaf said:
there was/is talk of how that may be introducing con tams via outside air being sucked in while steam is being released thru top vent.  is this true?



Hot air is less dense than cool air, and so as the PC cools it will "suck" air from the surrounding area. 

Autoclaves have compound gauges and DO NOT "suck" air back.  They will eventually enter into a "vacuum" and as the unit cools, negative pressure will rise UNTIL the user opens a relief valve.

If you use proper gas exchange/filter ports, this should introduce little to no issue.  Something as simple as a polyfill ge port should keep everything out.

I think what cronicr is explaining is that IF you start your plastic jars @ room temp, with no condensation then PC and allow to cool, that there should continue to be no condensation once it gets back to room temp.

Glass jars seem to work no matter what process.  I have yet to do anything special with the glass jars.  PC while hot/fluid etc.  I have done it a few ways with glass, and none of them have condensation after a day.  HOWEVER, I have noticed that the metal lids on glass jars produce slightly more condensate than the plastic lids.  All of which vanishes after 24 hours.


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19355935 - 01/01/14 07:13 PM (10 years, 28 days ago)

ok, i was reading GGMM and he mentions two kinds of PC/autoclave one of which will create a vacuum and the other which will not.  it suggests that if u have the one that does not create the vacuum to take the jars out right when pc drops to 0psi.  i have an AA 914 i believe (the one that fits 7 quarts) with that weight that comes off, is circular and have 5, 10, 15 holes.is the one i have creating  a vacuum?  i'd assume no, at least it doesn't seem like it when i open it, even with weight on top.

GGMM page 132, chapter 15 on generating grain spawn.

I'm asking because i let my jars cool in pc overnight.  whether i use postal tyvek or SFD, i can't help but think the filter is moist in that humid PC, and taking it out say an hour after it has dropped to 0 (so PC still hot and when weight is taken off steam vents for a few seconds) and letting jars cool on shelf would keep the filter dry.  maybe this is all in my head, the concern comes more so when using postal tyvek filters when i run out of SFD lids


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: blindingleaf]
    #19356192 - 01/01/14 08:43 PM (10 years, 28 days ago)

Quote:

blindingleaf said:
(so PC still hot and when weight is taken off steam vents for a few seconds) and letting jars cool on shelf would keep the filter dry.  maybe this is all in my head, the concern comes more so when using postal tyvek filters when i run out of SFD lids



This is how I do it, for the same reasons I believe.

I hate to keep a jar with a wet filter inside of a PC for hours after it cools.  I like you try to get them out ASAP, so that the filter can dry.

Hot jars fresh out of a pc will continue to vent hot air for a while I noticed.  Even though there is no pressure, the grains are still very hot.  Sometimes I can hear them "crying" as they are equalizing and eventually start pulling air back in as they cool.  I prefer to know that they are pulling through a dry filter vs a wet one.....Wet filters can transfer contaminants.  This is the way I've been doing it, and haven't had issues.


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19356414 - 01/01/14 10:21 PM (10 years, 28 days ago)

:crazy: whoa, turn your back for 2 hours and find out you missed school:crazy:
i use poly for my filter no foil during the pc have be doing so for god knows how many jars now without issues, the problem with releasing the pressure early is for exactly the reasons you stated and yes if you have a steilizers it always pulls a vacuum while it cools


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: cronicr]
    #19356480 - 01/01/14 10:42 PM (10 years, 28 days ago)

so whippy and chron...
are u guys waiting till PC cool to the touch to open or just till 0psi?  i remember several grain tek preps that stated to pull jar out a little after 0PSI to shake it and distribute moisture and no mention of possible danger, but then there are several more posts indicating the possible contam issue with this practice.... just made me wonder i guess which i should b doing particularly with the postal tyvek cause i trust my sfd no matter when they're taken out, have had 0 issue with them


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: blindingleaf]
    #19356504 - 01/01/14 10:48 PM (10 years, 28 days ago)

Quote:

blindingleaf said:

are u guys waiting till PC cool to the touch to open or just till 0psi?



@ 0 psi, my jars come out, and the foil comes off the lid.


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19356548 - 01/01/14 11:04 PM (10 years, 28 days ago)

Quote:

Whippy said:
Quote:

blindingleaf said:

are u guys waiting till PC cool to the touch to open or just till 0psi?



@ 0 psi, my jars come out, and the foil comes off the lid.



:thumbup: grain jar can come out, agar should stay in


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: cronicr]
    #19356576 - 01/01/14 11:16 PM (10 years, 28 days ago)

Quote:

cronicr said:

:thumbup: grain jar can come out, agar should stay in



My method for no-pour is the same as my grain.  At 0 psi, out they come.  This may be why my plastic jars always get condensate, while the glass ones are clean and clear.


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359682 - 01/02/14 05:09 PM (10 years, 27 days ago)

:smbfacepalm: just did me an oops....
i had a contaminated "plate" after a transfer and was doing up more agar, i decided tochuck the contammed one in the pc with the others...and forgot there was one contammed one in there and proceed to make more transfers....i can see the pc'd mold stuck in the agar lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Edited by cronicr (01/02/14 05:14 PM)


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: cronicr]
    #19359692 - 01/02/14 05:11 PM (10 years, 27 days ago)

it's either genius or :smbfacepalm:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
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Re: Pasty Agar Tek [Re: cronicr]
    #19359742 - 01/02/14 05:20 PM (10 years, 27 days ago)

Quote:

cronicr said:
it's either genius or :smbfacepalm:



I don't know where I put my 2 bacterial dishes that I re-pc'd....the only reason they got all iffy like that was because I wanted to test how sterile my microwave was.....

Ill let you know.  I have an idea they have something new growing on them.....


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359750 - 01/02/14 05:21 PM (10 years, 27 days ago)

i did that this morning and it just dawned on me a minute ago lol!


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359776 - 01/02/14 05:26 PM (10 years, 27 days ago)

Quote:

Whippy said:
Ill let you know.



Well, that was quick.  I found the 2 jars.

One was used for the first xfer from a MS inoculation, and grew out into a very close isolate.  The second dish by coincidence was a transfer from that dish, and is an isolate.

The only reason I know it was them 2 because they have the plastic lids and a slight murkiness to the agar.  Recycling!


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359790 - 01/02/14 05:28 PM (10 years, 27 days ago)

:crazy: all that really changed was the color of my agar, it got darker but i cn see the contam in the middle with the chunk from my transfer lol


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: cronicr]
    #19360255 - 01/02/14 07:03 PM (10 years, 27 days ago)

Quote:

cronicr said:
:smbfacepalm: just did me an oops....
i had a contaminated "plate" after a transfer and was doing up more agar, i decided tochuck the contammed one in the pc with the others...and forgot there was one contammed one in there and proceed to make more transfers....i can see the pc'd mold stuck in the agar lol



:bathtub40lol:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19360339 - 01/02/14 07:24 PM (10 years, 27 days ago)

:lmafo:
took me all fucking day to realize it, then i went and looked at them and was like WTF IS THAT IN THERE!:smbfacepalm:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleDelection
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Re: Pasty Agar Tek [Re: cronicr]
    #19370248 - 01/04/14 08:52 PM (10 years, 25 days ago)

You might want to check these out Pasty.

I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene).  This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.

I have run a test batch and they did hold up at 15psi for 15 min.  I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!). 

Here are some pics below.

Packaging



Size comparison



Measurements





Polypropelene stamp



120 of them neatly packed away in a 30x20x10cm little plastic bin.



--------------------
Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”


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