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aww
Stranger


Registered: 07/04/14
Posts: 44
Last seen: 9 years, 3 months
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How do you inoculate these? Do you remove the paper and stick the needle through the micropore tape?
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Pasty Agar Tek [Re: aww]
#20727606 - 10/20/14 04:10 AM (9 years, 3 months ago) |
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You could, but cracking the lid is probably a little easier than dealing with the tape in a SAB
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: aww]
#20728049 - 10/20/14 08:54 AM (9 years, 3 months ago) |
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Quote:
aww said: How do you inoculate these? Do you remove the paper and stick the needle through the micropore tape?
I just took my lid completely off inside my SAB. Granted I have no results on them yet, but a SAB should be fine!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Pretty much that. I loosen the lids on my donor and receiver plates and then I tilt the lid up, toward the ceiling of the SAB (so that it's less likely shit will land in it from above), get my x-acto in there and either cut a wedge and stab it, or just stab a pin with the tip, then in one sweeping motion, pull the inoculant out on the knife, close the first lid, crack the recipient lid and get the inoculant off of the knife or loop. Loops are probably easier for swiping shit. I tend to keep one side of the lid still on the plate so that I can both use it as a particle shield and put it back on easily.
I could use pointers on how to get shit off the tip of an x-acto knife though. I've kinda just been having to trust that the edge of the inside of the mini-round is clean and use it to slide the pin or wedge off. This does introduce a small amount of bacteria each time potentially though, which I've had manifest here and there. Certainly not to an unworkable extent by any means though. Good plates abound even with unrefined technique.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20728778 - 10/20/14 12:28 PM (9 years, 3 months ago) |
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I jammed a piece of agar into a transfer plate and it obliterated my transfer and the new plate's surface. It looks like an agar plane exploded and scattered across a field. Hella growth though! lol
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Pudgy]
#20728797 - 10/20/14 12:32 PM (9 years, 3 months ago) |
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Is it acceptable to just mash the inoculant stuck to the end of the knife into the middle of the new plate and then take it away? Lol
Sounds pretty cave-man like. What I need is a knife with a lever to eject what's on the end of it.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20728806 - 10/20/14 12:34 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: Is it acceptable to just mash the inoculant stuck to the end of the knife into the middle of the new plate and then take it away? Lol
Sounds pretty cave-man like. What I need is a knife with a lever to eject what's on the end of it.
I thought when I did it, that it was definitely savage-like and it made me laugh. I didn't see growth and thought I ravaged the poor myc. Then this morning, lots of growth -- resilient little buggers. I say -- MASH if you must man.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Pudgy]
#20728832 - 10/20/14 12:42 PM (9 years, 3 months ago) |
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I bet rubbing the freshly removed base of a pin on the middle of a plate would relieve the need to put the whole pin in there.
You know what would be balls-to-the-wall amazing? If I took a pin, stood it up straight on a new agar dish, and then it sent mycelium down into the agar and then kept maturing on schedule. About as likely as winning the lottery twice on consecutive days I'm sure.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20728841 - 10/20/14 12:45 PM (9 years, 3 months ago) |
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Why not bend the tip to an L so when you go to swipe the transfer it'll just come off into the new surface. I'd imagine trying to use a straight pin in a dish like the mini would be more difficult.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Pudgy]
#20728845 - 10/20/14 12:47 PM (9 years, 3 months ago) |
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I used these crude devices. I'm a mechanic -- I didn't even think to use a damn sewing pin, we have tons of those LOL.
http://www.harborfreight.com/4-piece-pick-and-hook-set-66836.html
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Pudgy]
#20728863 - 10/20/14 12:51 PM (9 years, 3 months ago) |
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Those aren't bad. I have guitar strings like was said a page or two back, and the x-acto. I'm really not at any shortage of stuff to use for that, but I'd like to stick with the knife for now.
When doing transfers I guess it's probably best to just swipe a little mycelium off the surface with the tip of the knife and then swipe it off in the same manner onto the new plate?
I transferred a whole fucking wedge my first few times, and that seems ridiculous to me haha.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Inocuole]
#20728879 - 10/20/14 12:54 PM (9 years, 3 months ago) |
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I thought the same thing, I did 4 transfers and I did each one differently.
Each one is growing differently too. Probably genetics.
However, one was a wedge, literally to the bottom of the cup like a small pie piece, a triangle. I put it on the new agar upside down with the myc side sandwiched between the two agar surfaces. It is growing VICIOUSLY, in one day is grew out and overtook the pie piece LOL? It's expanding outward too. I don't know if it liked being squished but I might try it again with another transfer to see if it does it again.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20728975 - 10/20/14 01:17 PM (9 years, 3 months ago) |
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Quote:
Inocuole said: I bet rubbing the freshly removed base of a pin on the middle of a plate would relieve the need to put the whole pin in there.
You know what would be balls-to-the-wall amazing? If I took a pin, stood it up straight on a new agar dish, and then it sent mycelium down into the agar and then kept maturing on schedule. About as likely as winning the lottery twice on consecutive days I'm sure.
Once I put a fairly developed pin on a fresh plate and it actually matured, dropped spores and ruined my chances of getting a clone culture from it
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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OH WHAT! A pin as in a mushroom? A fruit?
Dude, I thought he was talking about an actual sewing pin, a push pin... LOL!
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Quote:
Pastywhyte said:
Quote:
Inocuole said: I bet rubbing the freshly removed base of a pin on the middle of a plate would relieve the need to put the whole pin in there.
You know what would be balls-to-the-wall amazing? If I took a pin, stood it up straight on a new agar dish, and then it sent mycelium down into the agar and then kept maturing on schedule. About as likely as winning the lottery twice on consecutive days I'm sure.
Once I put a fairly developed pin on a fresh plate and it actually matured, dropped spores and ruined my chances of getting a clone culture from it 
Should have cut the cap off that bitch ha. Most of the pins I've used hadn't even formed veils yet.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Pastywhyte said:
Quote:
Inocuole said: I bet rubbing the freshly removed base of a pin on the middle of a plate would relieve the need to put the whole pin in there.
You know what would be balls-to-the-wall amazing? If I took a pin, stood it up straight on a new agar dish, and then it sent mycelium down into the agar and then kept maturing on schedule. About as likely as winning the lottery twice on consecutive days I'm sure.
Once I put a fairly developed pin on a fresh plate and it actually matured, dropped spores and ruined my chances of getting a clone culture from it 
Well fuck, put that way it sounds pretty undesirable. I would be pissed if that happened with this pin. I've had my AC off for like 90 minutes now just to let the air get reeeeal still in my work room.
Then again, being APE, the chances of it actually dropping spores are lower than the chances of them doing some other crazy shit, by the sound of it.
And yeah I call mushrooms pins and anything else is a needle, for consistency's sake.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20729320 - 10/20/14 02:43 PM (9 years, 3 months ago) |
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Yeah I got to that pin a little late but I still had hoped to make it work, usually invitro pins make good clones. I had a pic but I can't find it, might be on my comp at home.
I love how pp5 plates push out pins, look at this cluster 
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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One of MY John Allen clones pins on plates insanely fast. To bad it has shitty potency.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Pasty Agar Tek [Re: Pudgy]
#20731142 - 10/20/14 09:04 PM (9 years, 3 months ago) |
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Quote:
Pudgy said: I thought the same thing, I did 4 transfers and I did each one differently.
Each one is growing differently too. Probably genetics.
However, one was a wedge, literally to the bottom of the cup like a small pie piece, a triangle. I put it on the new agar upside down with the myc side sandwiched between the two agar surfaces. It is growing VICIOUSLY, in one day is grew out and overtook the pie piece LOL? It's expanding outward too. I don't know if it liked being squished but I might try it again with another transfer to see if it does it again.
I would not be surprised if you aren't growing mold with this one, IME, anything that can run a plate in one day usually is NOT mushroom mycellium, even thought it can be white.
I have done this a few times, but hey, we learn from are mistakes!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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I hope you're wrong. I can't afford to make a mistake, my self-esteem is teetering on a precipice of doom as it is.
In all honesty I might have exaggerated, it didn't run the entire plate -- just it's pie piece and it just finished colonizing that pie piece this morning. The weird part to me is that the transferred agar looked like whitish or dried out or something the past few days, it's now covered in mycelium and you can't even see the agar. It just looks like a fat little myc site.
The piece of agar wasn't very big, maybe 5% of the surface area of the mini round's floor. It looks like snow white healthy mycelium to me, but wtf do I know. I'll keep growing it for a while! All of my other plates are really doing well, no bacteria puddles, no off-whites and all growth sites look very similar to eachother. I'm really excited.
Edited by Pudgy (10/21/14 05:22 AM)
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