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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 18 hours
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What's the best method from going from dried spores to agar?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Re: Pasty Agar Tek [Re: Edmunter]
#20709127 - 10/16/14 12:44 AM (9 years, 3 months ago) |
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Quote:
Edmunter said: What's the best method from going from dried spores to agar?
This may not be the 'best' but it sure as hell is the easiest for me.
http://www.shroomery.org/forums/showflat.php/Number/20111637/fpart/all/vc/1
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 18 hours
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Thanks
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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I didn't think so.
About the dried spores, if your asking what I'm thinking, I am having the same dilemma, I have a dried out oyster mushroom, and I am going to take and swab it to see if I can't get any spores, then I'm going to take it and rehydrate it in distilled water, and see if I can't get some of the culture to spring back I hope that helps?
The only reason why I give a fuck about the oyster, was it was my first ever grow, and I let it die off, so I'm hoping I can save it
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: MudaFuka]
#20709171 - 10/16/14 01:01 AM (9 years, 3 months ago) |
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Quote:
MudaFuka said: Flame sterilize, make a hole with the hot needle then inject one drop into the hole without flaming the needle again. I find leaving my plates at room temp clears away the condensation better than putting them in the fridge. I just dump any excess water from my plate on the floor of my SAB before inoculating. Yes they can be kept in the fridge to halt growth.
Thanks Muda. I thought Pasty's Tek said to store them in the fridge? I'll take a few of them out right now to inoculate in the morning.
Also this may be a silly question, but how do I store them once I've inoculated them? Out in the open?
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Just somewhere out of your way, as long as your house is not superhot, or super cold.
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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I put all of mine in a closet. Until you get growth, and take what you need, then you can restore them back in fridge, it stalls the growth
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 18 hours
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would a sterilised ear cleaner be ok for swabbing?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: Edmunter]
#20709387 - 10/16/14 05:24 AM (9 years, 3 months ago) |
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I store mine in a glass replile tank next to my SAB untill they colonise then I put them in Ziploc bags and put them in one of my fridges crisper drawers. A sterilized qtip will work fine for a swab but I prefer to make an inoculating loop out of wire frome twist ties or a guitar string.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 18 hours
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Re: Pasty Agar Tek [Re: MudaFuka]
#20709396 - 10/16/14 05:35 AM (9 years, 3 months ago) |
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So im thinking of sterilising ear swabs for 20 minutes wrapped in foil.
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Delection
Spiritual Vagabond

Registered: 10/08/11
Posts: 848
Loc:
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Re: Pasty Agar Tek [Re: Edmunter]
#20710703 - 10/16/14 12:38 PM (9 years, 3 months ago) |
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This thread never stops, Pasty you got yourself a hit on your hands
-------------------- Life should not be a journey to the grave with the intention of arriving safely in a pretty and well preserved body, but rather to skid in broadside in a cloud of smoke, thoroughly used up, totally worn out, and loudly proclaiming “Wow! What a Ride!”
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pawnzy
That Guy

Registered: 03/04/14
Posts: 1,030
Last seen: 6 years, 10 months
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Re: Pasty Agar Tek [Re: Edmunter]
#20710801 - 10/16/14 12:58 PM (9 years, 3 months ago) |
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Quote:
Edmunter said: So im thinking of sterilising ear swabs for 20 minutes wrapped in foil.
Why not get the pre sterilized swabs?
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Pasty Agar Tek [Re: pawnzy] 1
#20710906 - 10/16/14 01:23 PM (9 years, 3 months ago) |
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Quote:
pawnzy said:
Quote:
Edmunter said: So im thinking of sterilising ear swabs for 20 minutes wrapped in foil.
Why not get the pre sterilized swabs?
Maybe because ear swabs are like $2 for 500 and he's already got foil and the PC around, as opposed to buying them online and paying for shipping for such a minuscule purchase. Use what you got I reckon.
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pawnzy
That Guy

Registered: 03/04/14
Posts: 1,030
Last seen: 6 years, 10 months
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i got mine at the pharmacy for 17 cents each..guess so.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Delection said: This thread never stops, Pasty you got yourself a hit on your hands 
I know it's awesome. Lots of good info here in the comments as well
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 18 hours
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Quote:
MrGiraffe said:
Quote:
pawnzy said:
Quote:
Edmunter said: So im thinking of sterilising ear swabs for 20 minutes wrapped in foil.
Why not get the pre sterilized swabs?
Maybe because ear swabs are like $2 for 500 and he's already got foil and the PC around, as opposed to buying them online and paying for shipping for such a minuscule purchase. Use what you got I reckon.
I want to do it tomorrow. no chance for them get to me quick enough........ sterilising is sterilising in my book.... I just wanted to make sure I wasnt wasting my time
And yes Pasty you are a fukin Leg End
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: Edmunter]
#20711570 - 10/16/14 03:37 PM (9 years, 3 months ago) |
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Quote:
Edmunter said: And yes Pasty you are a fukin Leg End
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
Imperfect Iam said: I put all of mine in a closet. Until you get growth, and take what you need, then you can restore them back in fridge, it stalls the growth
Ok, cool. What do I need covering them after I inoculate and set out in my closet?
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Just the lids, and the mp tape, or whatever you used to cover the hole, this stuff is not half as hard as it seems, I know this sounds strange, but this is how I started, with poured plates thought, I had all the wrong equipment, and I ended up seeing damn near EVERY contam. You can get. With this setup, you will rarely catch a contam. Unless your sterile procedure is off.
I was afraid to make the jump to grain, LOL.
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Sweet. So just take off the foil and paper towel, inoculate, and toss that shit in my closet somewhere. Neato. I'll do that later tonight and post pictures when it starts growing.
There's tons of random mushrooms growing in my neighborhood field. Maybe I'll take a spore print and practice inoculating with those shits.
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