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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 20 hours
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Re: Pasty Agar Tek [Re: Pudgy]
#20706302 - 10/15/14 01:05 PM (9 years, 3 months ago) |
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Ok so ive taken Pasties advice and used a 10:7:2:1 Agar:potato starch:yeast. Im hoping for quicker growth so Ive just finished 6 transfers from 2 cultures and im hoping to get 2 plates to use the tiger tip and the other 4 to get some isolates.
Im still having trouble with condensation and I have no idea why.
Im hoping to put an end to my high contam rate with my new SAB
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 20 hours
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Re: Pasty Agar Tek [Re: Edmunter]
#20706306 - 10/15/14 01:06 PM (9 years, 3 months ago) |
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Good timing Mr G
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newshroomer9901
Stranger



Registered: 06/15/12
Posts: 254
Loc: USA
Last seen: 3 years, 6 months
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Re: Pasty Agar Tek [Re: Edmunter]
#20706317 - 10/15/14 01:09 PM (9 years, 3 months ago) |
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Guess I'll start out with that thanks for that help!
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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petris come sterile.
this is an all in one sterilize no pour agar tek
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Pasty Agar Tek [Re: Edmunter]
#20706454 - 10/15/14 01:48 PM (9 years, 3 months ago) |
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Quote:
Edmunter said: Ok so ive taken Pasties advice and used a 10:7:2:1 Agar:potato starch:yeast. Im hoping for quicker growth so Ive just finished 6 transfers from 2 cultures and im hoping to get 2 plates to use the tiger tip and the other 4 to get some isolates.
Im still having trouble with condensation and I have no idea why.
Im hoping to put an end to my high contam rate with my new SAB

It seems no matter what I do, I end up with condensation (no pour pp5s and disposable petris). I used to use the paper towel that was under the foil to wipe out the excess condensation on the pp5s, and some people will dump out the water on to the floor of the SAB before inoculation. With the poured dishes, if I keep them stacked and use the hot water in a coffee mug on top, I still end up with condensation on the sides. Temp swings in my house also contribute to the problem. Usually within a few days though, the moisture has all dried up in the poured dishes.
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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I see. So I thought it was my crappy durapore tape that was causing my condensation. Pasty told me what you just said "it'll dry up in a few days".
Dumping the water/wiping before inoc sounds like a good idea. I'm going to try again tonight with the ziploc screw tops and this time I'll dump/wipe before inoc.
I'm also going to do a controlled dish to test the sterility of my environment.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Pasty Agar Tek [Re: Pudgy]
#20706875 - 10/15/14 03:18 PM (9 years, 3 months ago) |
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Condensation, this has been the one big bitch about this tek. In petri's that you pour, they do seem to dry up quicker, but as I said, I use the screw top PP5's, for some reason they do build up more condensation it seems, but all this is really a non issue to me, when you inoc. Slowly turn it over in your SAB and dump it out if it's bothering you that bad, this is what I do with no problems that I can recall ever being caused by this.
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Here's some pics from my first attempts at this Tek last night:

I tried a bunch of variations of waterproof surgical tape and a slice of Tyvek from my suit since I had no micropore tape. Only time will tell if they worked, but at least none of them exploded in there.

I ground the agar brick in a coffee grinder into this, which I hope is a decent size.

This was just about 2.5 grams. I snapped a picof it in the container so you guys can ballpark your own if you don't have a scale.

I think this is where I put the drop of Karo in.

This is as fine as I got the first batch. I put the second one on a higher setting to get it finer since Pasty's looked better.

The consistency.

I let it cool within the pan which made it coagulate and become hard to deal with. I heated it up again so it was easier to spoon in. For the next batch, I spooned them in hot and let them cool in the containers, which I think is a better idea.

Foiled up.

This is the second batch which came out a bit better.
Critique anything and help me out!
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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I can't really say a lot about the bar agar, I have only worked with powder, but I will say I think you should be ok with the way that last one looks, if they do not work, WHO CARES, you tried, that is the main thing, I can already see you getting better from your first try, to your second, the third should be great, but I'm betting those work, as long as that last pic is not sitting out in open air after you PC'd!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Haha, that's before PC and they're all in my fridge now waiting patiently like good little agar cups. And yup, like you said, it's no big deal if they're fucked. I kinda wish I would've read the measurements better before shopping. I didn't realize I needed literally a drop of Karo, 2 teaspoons of potato crap and 2.5g of agar blocks. I now have a lifetime supply of everything LOL (which is nice).
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Pasty Agar Tek [Re: Edmunter]
#20707275 - 10/15/14 05:03 PM (9 years, 3 months ago) |
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Quote:
Imperfect Iam said: I have never had the problem of to stiff agar, but have had it not stiff enough, using the measurements from the v-tek, grain water agar, it made it really suck to try and transfer
Think I said it elsewhere too, but your agar gelling problems are not due to grain water agar. The agar-agar measurements I gave are no different than for any other agar mixture. 2% agar weight to liquid volume.
Quote:
Edmunter said: So what does agar do when its too stiff or not stiff enough?
"Too" stiff, it just makes it super firm. It can kindof seem dry by relation, but mycelium can grow on it too. If it is WAY too hard it may support only thin growth. But mostly it's just a waste of materials. If it's not hard enough, jell-o soft, it makes it a pain to transfer if not impossible. But there are a few little-known grow techniques that involve using very soft agar at just like 1/4th of the agar (.5% weight to liquid volume) and mycelium grows on it wonderfully.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: Violet]
#20707310 - 10/15/14 05:10 PM (9 years, 3 months ago) |
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I currently only have spore syringes right now. I've looked around and heard mixed things about inoculating via syringe, specifically whether I should use a loop instead and going under the agar versus on top of it.
Anyone have any solid advice and if possible, a good video showing agar inoculation with MS syringe?
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Pudgy
Fail-2-Win

Registered: 09/30/14
Posts: 657
Last seen: 8 years, 9 months
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Re: Pasty Agar Tek [Re: Violet]
#20707560 - 10/15/14 05:55 PM (9 years, 3 months ago) |
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Quote:
Violet said:
Quote:
Edmunter said: So what does agar do when its too stiff or not stiff enough?
"Too" stiff, it just makes it super firm. It can kindof seem dry by relation, but mycelium can grow on it too.
Thanks for that answer, I was hoping to read something like that.
Does the mycelium grow through the agar or just on top of it?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: Pudgy]
#20708882 - 10/15/14 11:20 PM (9 years, 3 months ago) |
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Quote:
Anyone have any solid advice and if possible, a good video showing agar inoculation with MS syringe?
just a single drop?? i never had problems with that. maybe try to keep ur pasty plate level when u move it from SAB>colonization if u really are paranoid. otherwise, sterilize a q tip, squirt a single drop or two onto it, and then "swipe" that onto the pasty plate. pasty plates have higher walls than a normal plastic petri, so u do not have to worry as much about germination around the perimeter.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I like to carve a little divot in the center of my plate with the flame sterilized needle. Then squeeze a drop into the divot.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: MudaFuka]
#20709042 - 10/16/14 12:15 AM (9 years, 3 months ago) |
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Thanks.
1. Ok, so flame sterilize, carve out a little hole, wipe off and sterilize again, and then inject what? A few drops?
2. My agar cups are still in the fridge with condensation around them. I'm assuming that won't change so I just need to use them and flick/dump out the water in my SAB?
3. If I successfully colonize the agar cups, can I toss it back in the fridge for a while to slow things down or pause the growth? I don't have the other supplies yet to do a bulk grow.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Flame sterilize, make a hole with the hot needle then inject one drop into the hole without flaming the needle again. I find leaving my plates at room temp clears away the condensation better than putting them in the fridge. I just dump any excess water from my plate on the floor of my SAB before inoculating. Yes they can be kept in the fridge to halt growth.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
Imperfect Iam said: Condensation, this has been the one big bitch about this tek.
I hear a lot of bitching but I don't hear a lot about increased contamination or problems arising because of it.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Right, that's what I was getting at in the post, if you read further down, I said if it bothers you so much dump it out, that's what I do, with no problems that I can recall
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
Imperfect Iam said: Right, that's what I was getting at in the post, if you read further down, I said if it bothers you so much dump it out, that's what I do, with no problems that I can recall
I know it wasn't directed at you specifically.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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