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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20704799 - 10/15/14 12:52 AM (9 years, 3 months ago)

I'm about to do this and am curious about the corn syrup. When he says add a drop, is that a figure of speech or does he literally mean a drop?


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InvisibleJohnnyTripsAlot
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20704848 - 10/15/14 01:20 AM (9 years, 3 months ago)

nice tek, ty


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InvisibleImperfect Iam
^means imperfect,not I'm perfect
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Registered: 03/05/13
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20705032 - 10/15/14 04:05 AM (9 years, 3 months ago)

No, he really means a drop, if you happen to get a hair more, I think you will be ok,I'm almost 100% on.


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


Edited by Imperfect Iam (10/15/14 04:16 AM)


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Imperfect Iam]
    #20705098 - 10/15/14 05:28 AM (9 years, 3 months ago)

Ok, thanks Iam. I did like 2 drops in the first batch I made and 1 in the second, so I should be okay. My batches came out way smaller though even though I was pretty accurate with the measurements. I had to make 2 batches to get 8 little cups worth. Only time will tell!


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Offlinepawnzy
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Registered: 03/04/14
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20705301 - 10/15/14 08:08 AM (9 years, 3 months ago)

Quote:

stareatclouds said:
Ok, thanks Iam. I did like 2 drops in the first batch I made and 1 in the second, so I should be okay. My batches came out way smaller though even though I was pretty accurate with the measurements. I had to make 2 batches to get 8 little cups worth. Only time will tell!




You probably used too much then, this recipe is perfect for 8 mini rounds. You just have to cover the bottom of the round.

I use two drops of honey usually though, but i usually make a quadruple batch for 32 plates so


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: pawnzy]
    #20705875 - 10/15/14 11:21 AM (9 years, 3 months ago)

Dang, only time will tell. I'm going to go get them out soon and seen.


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OfflinePudgy
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Registered: 09/30/14
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Re: Pasty Agar Tek [Re: stareatclouds]
    #20705907 - 10/15/14 11:29 AM (9 years, 3 months ago)

Quote:

stareatclouds said:
Dang, only time will tell. I'm going to go get them out soon and seen.




I actually used this stuff called "Durapore" tape.



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How To Use The Search Function


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OfflinePudgy
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Re: Pasty Agar Tek [Re: Pudgy]
    #20705909 - 10/15/14 11:30 AM (9 years, 3 months ago)

So... I didn't use the search function before using my "Durapore" tape. I felt the word "pore" meant it would breathe... :rolleyes:

Quote:

Omnicracker said:
i just bought some of the transpore tape yesterday, and i the same dilema, but its not that complicated.

Durapore is not breathable, so dont use it. 
Transpore is breathable, plastic, and its also stretchy and easy to use.
Micropore is also breathable, but the tape itself was paper tape, i thought that would be bad.

i bought tht transpore and it didnt hold up on the PC. go with the mircopore, paper cant melt. 
i was using it for LC and i thoght that the paper might get soggy when i shook the jar.




I found this in a search.


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How To Use The Search Function


Edited by Pudgy (10/15/14 11:52 AM)


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Offlinenewshroomer9901
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Re: Pasty Agar Tek [Re: Pudgy]
    #20706077 - 10/15/14 12:08 PM (9 years, 3 months ago)

If I was to use this tek with petri dishes would you still pressure cook the dishes?


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: newshroomer9901]
    #20706094 - 10/15/14 12:14 PM (9 years, 3 months ago)

if they are plastic, they will melt inside the PC.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinenewshroomer9901
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Re: Pasty Agar Tek [Re: blindingleaf]
    #20706121 - 10/15/14 12:20 PM (9 years, 3 months ago)

How would you use plastic petris with agar? Or can you at all


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InvisibleMrGiraffe

Registered: 04/04/14
Posts: 3,149
Re: Pasty Agar Tek [Re: newshroomer9901]
    #20706167 - 10/15/14 12:35 PM (9 years, 3 months ago)

Make your agar in a bottle, PC, then pour your agar into dishes in a SAB or in front of a flow hood.  But this is the NO pour method.


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OfflinePudgy
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Re: Pasty Agar Tek [Re: MrGiraffe]
    #20706180 - 10/15/14 12:37 PM (9 years, 3 months ago)

Quote:

MrGiraffe said:
Make your agar in a bottle, PC, then pour your agar into dishes in a SAB or in front of a flow hood.  But this is the NO pour method.




Why is it called the no pour method when you pour it?


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How To Use The Search Function


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Offlinespacechildo
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Re: Pasty Agar Tek [Re: Pudgy]
    #20706185 - 10/15/14 12:39 PM (9 years, 3 months ago)

huh? you dont pour it. there are no petris in this tek.


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OfflinePudgy
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Re: Pasty Agar Tek [Re: spacechildo]
    #20706189 - 10/15/14 12:41 PM (9 years, 3 months ago)

Well, with this tek I scooped with a spoon. I guess that's not pouring. I guess I got confused by what Mr. Giraffe said. Perhaps he explained the 'pour method' in the first sentence and then "But this is the NO pour method" was referring to Pasty's tek?


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How To Use The Search Function


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: spacechildo]
    #20706196 - 10/15/14 12:43 PM (9 years, 3 months ago)

I just took mine out of PC and they had condensation all throughout. I guess I didn't let them cool enough? I brought the pressure cooker up to 15PSI as slow as I could. They were all a bit damp on the bottom which I guess is normal if condensation is present. Into the fridge they go.

Is there a way to tell if everything went smoothly other than inoculating and looking?


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Offlinespacechildo
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Re: Pasty Agar Tek [Re: Pudgy]
    #20706198 - 10/15/14 12:44 PM (9 years, 3 months ago)

yeah giraffe explained to the other dude how to use plastic petris. and then told him this is not a plastic petris tek.

pour = pour agar after its PC'd
no pour = PC agar in separate containers.

the agar mix wont magically go from your pot to the containers so in that sense you gotta pour it (or spoon it) :lol:


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OfflinePudgy
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Re: Pasty Agar Tek [Re: spacechildo]
    #20706211 - 10/15/14 12:46 PM (9 years, 3 months ago)

:thumbup:


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How To Use The Search Function


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Offlinenewshroomer9901
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Re: Pasty Agar Tek [Re: spacechildo]
    #20706273 - 10/15/14 12:59 PM (9 years, 3 months ago)

I gotcha. Is there anything that much better about using the pour method vs no pour?


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InvisibleMrGiraffe

Registered: 04/04/14
Posts: 3,149
Re: Pasty Agar Tek [Re: newshroomer9901]
    #20706289 - 10/15/14 01:03 PM (9 years, 3 months ago)

The pour method into disposable petri dishes has better clarity as opposed to looking through the blue lid, or opaque pp5 container.  No pour is definitely where it's at if you're an agar rookie, good way to get your feet wet.  There are condensation issues with both ways.


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