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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Pasty Agar Tek *DELETED* [Re: kmetric]
#20597232 - 09/21/14 01:38 PM (9 years, 4 months ago) |
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Post deleted by Sanguin3
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 30 days
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Re: Pasty Agar Tek [Re: Sanguin3]
#20597237 - 09/21/14 01:40 PM (9 years, 4 months ago) |
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solved it for me! good luck on the next batch and let us know how it worked out.
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kmetric



Registered: 08/23/14
Posts: 140
Last seen: 7 years, 10 months
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Re: Pasty Agar Tek [Re: Sanguin3]
#20597238 - 09/21/14 01:41 PM (9 years, 4 months ago) |
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Will do thanks fellas
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Pasty Agar Tek *DELETED* [Re: kmetric]
#20622315 - 09/26/14 04:51 PM (9 years, 4 months ago) |
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Post deleted by Sanguin3
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: Sanguin3]
#20622329 - 09/26/14 04:54 PM (9 years, 4 months ago) |
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I think its less of an issue with pasty plates. If it were a big issue the tiger drop wouldn't be a good idea and people seem fine with it. I'm not sure if it is a good idea to let your plates consolidate or not. I know I haven't had a problem using plates as soon as the growth hits a good size. I have just been cutting a circle around the mycelium.
Edited by MudaFuka (09/26/14 04:55 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: Sanguin3]
#20622362 - 09/26/14 05:01 PM (9 years, 4 months ago) |
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I'm curious what pasty has to say too!! i think pasty plates are safer around the edges than normal plates, but he uses them way more so i'd trust his judgement.
that said, bacteria should not start forming at the edge of regular plates, mold will do that. if u get bacteria on plates, its usually the innoculant. mold can come from the innoculant, but also from bad sterile technique (too much movement in SAB/not flaming blade till red hot). maybe bacteria can sneak in i guess, but its usually a tactile thing, as opposed to a floating in the air/getting thru the parafilm type thing.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
I know I haven't had a problem using plates as soon as the growth hits a good size. I have just been cutting a circle around the mycelium.
thats what i do as well.
but i agree, as per the tiger drop, it should not be a problem.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Sanguin3 said: This question got skipped over in muda's LI thread so I figured I'd ask it here, I was curious about the same thing.
Quote:
Mr. Alien said: So pasty... you think Is better use full colonised plates and let them consolidate a bit for LI? If so... when you cut the wedge, you just cut the wedge in a circle form and avoid the agar of the edges....?
Do you still get a higher rate of bacteria on the edges of the mini rounds or does that only happen in petris?
Quote:
blindingleaf said:
Quote:
I know I haven't had a problem using plates as soon as the growth hits a good size. I have just been cutting a circle around the mycelium.
thats what i do as well.
but i agree, as per the tiger drop, it should not be a problem.
Exactly. I have had a couple satelites out of hundreds of plates but, I am sure that was my fuck up not the plate.
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Sanguin3
Optimist

Registered: 10/19/13
Posts: 2,273
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Re: Pasty Agar Tek *DELETED* [Re: Pastywhyte]
#20623072 - 09/26/14 08:15 PM (9 years, 4 months ago) |
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Post deleted by Sanguin3
Reason for deletion: .
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Re: Pasty Agar Tek [Re: Sanguin3]
#20623274 - 09/26/14 08:51 PM (9 years, 4 months ago) |
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I just recently had my first contamination on a spore-swiped plate and it was green mold that began as a little satellite at the very edge of the dish.
I think the contamination is always going to be more common at the edges by virtue of design.
At first I waited till the plates fully colonized before I dropped them. I used to think of the mycelium as kind of a shield for the agar.
But now I simply see the agar as a delivery mechanism for the mycelium. I've even thought about just swabbing spores onto the plates and then dropping the agar in before seeing any growth at all to see what happens.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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jerseyian
Friend


Registered: 10/19/13
Posts: 1,560
Last seen: 6 years, 10 months
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I tried doing my own agar again, this time I got the agar to solidify. I used agar, potatoe flakes water and some karo. I poured the agar into jars, capped them and PC them for 15-20 mins. When the cooled I looked in and there is condensation inside the jars, like too much. What am I doing wrong?
-------------------- YEP I AM TREE HUGGING HIPPIE Have... several different prints. Want to trade any ethnobotanical seeds? I have lots.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Are you using jars? Cause they are terrible for that. One of the big reasons I started to experiment with pp5 plastic in the first place.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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Quote:
jerseyian said: I tried doing my own agar again, this time I got the agar to solidify. I used agar, potatoe flakes water and some karo. I poured the agar into jars, capped them and PC them for 15-20 mins. When the cooled I looked in and there is condensation inside the jars, like too much. What am I doing wrong?
it will dry out in a few days
Edited by tripdawg420 (09/29/14 08:46 PM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
Pastywhyte said: Are you using jars? Cause they are terrible for that. One of the big reasons I started to experiment with pp5 plastic in the first place.
I use glass jars. pints can be awful with a lot of condensation along the sides, while shorter and closer to half pint jars work well. I use poly lids tho. and the TP is very important as you said to me in an earlier post when I had forgot it!
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jerseyian
Friend


Registered: 10/19/13
Posts: 1,560
Last seen: 6 years, 10 months
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Yes I used small mason jars. Someone replied and said it will dry out. You had said to use the gladware platic ones and I did, they collapsed inward.
-------------------- YEP I AM TREE HUGGING HIPPIE Have... several different prints. Want to trade any ethnobotanical seeds? I have lots.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Did you put GE holes in the lids? I've got glad mini rounds that have been threw dozens of runs.
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jerseyian
Friend


Registered: 10/19/13
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Last seen: 6 years, 10 months
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Someone said to try jelly jars, they have a pop off top. I was trying mason jars with holes in the lids and surgical tape over the holes so I could inject the spores right through the lid but decided it would be hard as hell to get just a drop or two that way. I am going to try something with a pop off lid and do it in a still air box. Also there is way too much condensation in the ones I did in the mason jars and it didn't dry out in a week. I could open the lids and pour it out but there goes my sterile environment.
-------------------- YEP I AM TREE HUGGING HIPPIE Have... several different prints. Want to trade any ethnobotanical seeds? I have lots.
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jerseyian
Friend


Registered: 10/19/13
Posts: 1,560
Last seen: 6 years, 10 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20637802 - 09/29/14 09:24 PM (9 years, 3 months ago) |
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yes but I used the wrong tape I think. What kind should I use for GE? Do you pop open the lids in a still air box to drop the spores in?
-------------------- YEP I AM TREE HUGGING HIPPIE Have... several different prints. Want to trade any ethnobotanical seeds? I have lots.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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In the past when I have had to much condensation on my plates I just dumped the excess water onto the floor of my SAB right before inoculating them.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Pasty Agar Tek [Re: MudaFuka]
#20637904 - 09/29/14 09:47 PM (9 years, 3 months ago) |
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Quote:
MudaFuka said: In the past when I have had to much condensation on my plates I just dumped the excess water onto the floor of my SAB right before inoculating them.
^^^^^^^^^^
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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