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 CapMeh
RX Jazzist
Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 5 months, 13 days
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 Re: Pastywhyte's Easy Agar Tek  [Re: FunnelMind]
#27077873 - 12/07/20 09:24 PM (3 years, 4 months ago) |
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Quote:
FunnelMind said:
So, I used your TEK, and I have 3 different types of mushrooms that I inoculated via spore prints, in an SAB box. This one has the most growth.
I have grown mushrooms in the past, but this is my first attempt at agar. I was concerned at first that I might have cobweb mold, since it is so hard to see the growth in the agar and I didn't use food colouring. After seeing some other posts, I believe this is mycelium, especially since all the glad rounds that I inoculated look the same way, and the little black spots in the middle of the growths are the groups of spores that I scraped off the spore print, into the agar, when I inoculated them. In all of my glad rounds the growth is growing outwards, away from the spores (black spots in pics).
It has been 11 days since I inoculated the agar. Do you think I just over did it with the spores, and that I have a bunch of competing mycelium? Do you think I can transfer to grain in a couple of days? Any thoughts or suggestions would be greatly appreciated.
Thank You!
Going from spore to agar to grain without transferring seems a lot like going from spore straight to grain to me. The point of spore to agar is to clean the desirable mycelium through transfers. Do some transfers from the leading edge of your colonies and post your results is all the advice I can offer. I’m new to germ plates and just transferred from my first, I took 4 transfers from the best looking germ plate. Tried to select different looking mycelial growth, and the results will give me more insight into what was actually happening on the germ plate.
I also suggest diving into the well of knowledge provided by the fellow above, PastyWhyte. He is my agar guru <3
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 EastBayRay
Registered: 06/06/13
Posts: 746
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Re: Pasty Agar Tek *DELETED* [Re: CapMeh]
#27080649 - 12/09/20 02:57 PM (3 years, 4 months ago) |
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Post deleted by EastBayRay
Reason for deletion: d
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc: 
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Quote:
EastBayRay said:
I know this has been asked before but I do not know how to search this thread... how long to steam sterilize Pastyplates without a PC like we do with PF Tek?
45 minutes is plenty IME.
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EastBayRay
Registered: 06/06/13
Posts: 746
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Re: Pasty Agar Tek *DELETED* [Re: Josex]
#27080653 - 12/09/20 03:00 PM (3 years, 4 months ago) |
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Post deleted by EastBayRay
Reason for deletion: d
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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45 minutes is what I used to do before having a PC and I had no problems. With a PC and depending on the media used I sterilize from 20 to 30 minutes, mileage may vary depending on the altitude you live.
Give it one hour if you want, it won't damage anything and peace of mind is a good thing.
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adhoc
Registered: 05/03/20
Posts: 167
Last seen: 1 year, 10 months
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Quote:
EastBayRay said:
I know this has been asked before but I do not know how to search this thread
Just for reference - it's not very obvious but at the very bottom of the page is a search box with the label "Search this thread".
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EastBayRay
Registered: 06/06/13
Posts: 746
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Re: Pasty Agar Tek *DELETED* [Re: Josex] 1
#27081180 - 12/09/20 08:35 PM (3 years, 4 months ago) |
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Post deleted by EastBayRay
Reason for deletion: fd
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Josex
#cheat_code
Registered: 11/13/15
Posts: 8,996
Loc:
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You got me there, nice searching skillz 
That's what I did in the beginning yes, but I cut the time down more and more over time until I realized 45 minutes were enough.
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waxbrain
That Fun Guy
Registered: 05/13/17
Posts: 678
Last seen: 3 years, 16 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#27087458 - 12/13/20 03:19 PM (3 years, 4 months ago) |
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so im about to turn off my stove and start letting the PC cool. i saw that you typically allow it to cool overnight - how long should i wait before opening it up since its only 5pm right now? should i still wait till the morning to take them out or is a few hours fine?
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Wangsta79
Mycology Enthusiast
Registered: 05/25/20
Posts: 2
Loc: Utah
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Re: Pasty Agar Tek [Re: teknix]
#27087543 - 12/13/20 04:21 PM (3 years, 4 months ago) |
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Log in to view attachment
I found a 12 pack of 1/2 pint Mason jars at smith's for only like $15 or less after tax. That's not a bad price considering your buying glass jars that can be reused indefinitely. Just thought I would mention it in for those wanting some kind of glass containers for their agar storage dishes
Edited by Wangsta79 (12/13/20 04:37 PM)
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CapMeh
RX Jazzist
Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 5 months, 13 days
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Quote:
waxbrain said:
so im about to turn off my stove and start letting the PC cool. i saw that you typically allow it to cool overnight - how long should i wait before opening it up since its only 5pm right now? should i still wait till the morning to take them out or is a few hours fine?
Letting them cool overnight is my suggestion.
Quote:
Wangsta79 said:
I found a 12 pack of 1/2 pint Mason jars at smith's for only like $15 or less after tax. That's not a bad price considering your buying glass jars that can be reused indefinitely. Just thought I would mention it in for those wanting some kind of glass containers for their agar storage dishes
I won't ask what region you're in, but that's standard price for mason jars in mine.
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waxbrain
That Fun Guy
Registered: 05/13/17
Posts: 678
Last seen: 3 years, 16 days
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Re: Pasty Agar Tek [Re: CapMeh]
#27088407 - 12/14/20 08:54 AM (3 years, 3 months ago) |
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so when i woke up this morning and took out my plates i noticed i messed up and didnt have some of the containers flat enough. is the agar being tilted going to give me any serious issues or is it workaroundable?
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Pastywhyte 
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada 
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Re: Pasty Agar Tek [Re: waxbrain]
#27088622 - 12/14/20 11:14 AM (3 years, 3 months ago) |
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It might be a pain if you’re dropping spore solution on it however it will still work. It won’t even make a slight difference for transfers or using a spore loop. I don’t worry about slanted agar myself.
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Ababyphoenix
You
Registered: 03/21/19
Posts: 1,015
Loc: Human Body
Last seen: 1 month, 25 days
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My first round of agar turned out like this. Didnt seem to hurt much
 he made the tek lol
--------------------
     Pho's MushiAventures:
 My '21 GLOG  LAGM 2022
Edited by Ababyphoenix (12/14/20 11:17 AM)
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CapMeh
RX Jazzist
Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 5 months, 13 days
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Quote:
Pastywhyte said:
It might be a pain if you’re dropping spore solution on it however it will still work. It won’t even make a slight difference for transfers or using a spore loop. I don’t worry about slanted agar myself.
Have you noticed a difference in the look of the myc on a slanted plate? When mine are slanted I seem to get more rhyzo on the thin side and more fluffy/cottony on the thick side. Does this sound reasonable?
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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Re: Pasty Agar Tek [Re: CapMeh]
#27088962 - 12/14/20 02:32 PM (3 years, 3 months ago) |
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Quote:
CapMeh said:
Quote:
Pastywhyte said:
It might be a pain if you’re dropping spore solution on it however it will still work. It won’t even make a slight difference for transfers or using a spore loop. I don’t worry about slanted agar myself.
Have you noticed a difference in the look of the myc on a slanted plate? When mine are slanted I seem to get more rhyzo on the thin side and more fluffy/cottony on the thick side. Does this sound reasonable?
Yes, I assume due to a lower concentration of nutes in the thin side. A small gradient likely forms.
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infestedpasta
Stranger
Registered: 09/20/07
Posts: 128
Last seen: 18 hours, 7 minutes
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I wasn't really paying attention and I added probably 5 - 10 drops of honey... Should I use this Agar or start over?
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,842
Loc: Canada
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Yeah that much simple sugar isn’t a good thing. I would start over myself.
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infestedpasta
Stranger
Registered: 09/20/07
Posts: 128
Last seen: 18 hours, 7 minutes
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Ok thanks... I re made some last night.
I took 3 tissue samples and used 2 plates to test two different liquid cultures i made..
Only thing I did different is I used these Tupperware containers instead, it was all I could find.
Should still work right?
I'll let you know how it goes
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CapMeh
RX Jazzist
Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
Last seen: 5 months, 13 days
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Edited by CapMeh (01/26/21 03:01 PM)
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