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OfflineVoiceOfLogos


Registered: 01/31/17
Posts: 71
Last seen: 3 years, 3 months
Re: Pasty Agar Tek [Re: JHOVA]
    #26049737 - 06/13/19 08:33 AM (4 years, 7 months ago)

Use jar lids lots of jar lids then put your pc liner/bottom/trivet ontop of those, above water. Then you can even use some foil balls around the plates for extra support if your worried they might tip.


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OfflineGearoidy
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Registered: 11/20/18
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Re: Pasty Agar Tek [Re: VoiceOfLogos]
    #26070114 - 06/23/19 08:57 PM (4 years, 7 months ago)

This is excellent thank you


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OfflineMagicJim
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Registered: 08/23/17
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Re: Pasty Agar Tek [Re: Gearoidy] * 1
    #26071874 - 06/24/19 09:02 PM (4 years, 7 months ago)

My first plates  :rockon:


Need to pickup some food coloring


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Offlineshroomator
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Re: Pasty Agar Tek [Re: MagicJim]
    #26101910 - 07/11/19 09:57 AM (4 years, 6 months ago)

Anyone tried hole in the side with Polyfill?
Should work if micropore works right?


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OfflineLevster
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Registered: 08/17/16
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Re: Pasty Agar Tek [Re: shroomator]
    #26109921 - 07/15/19 03:42 PM (4 years, 6 months ago)

I've used this tek before, it was good. I did it again, but for some reason all the mini round lids that were in the PC do NOT tighten properly anymore. the heat must have warped them, or they must have changed the products in the plastic in the last 3 years, because I can just lift it right off the damn container. Oh well, still inoculated. (only pc'd for 40 minutes, slow build up, didn't go super hot) Not sure why this happened. =/


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Offlineblackout
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Re: Pasty Agar Tek [Re: Levster]
    #26153656 - 08/26/19 12:53 PM (4 years, 5 months ago)

I mentioned microwaving plates with lots of condensation before, just for 5-10seconds, this remelts a spot of agar which will usually result in it mixing with the free/liquid water and then it will set leaving far less liquid water -if any. I now put something under my jars on one side so it is tilted to one side, I just now used a plastic pen lid. This tilting makes the liquid all gather in one side. Now when microwaved the microwaves appear to all go into that one spot where the water has gathered, and remelts only there first. If you do it flat it will randomly melt a small spot, probably where the water is gathered slightly more. After remelting and setting it leaves an uneven surface so I would prefer to have this at the side of a jar rather than a random spot(s) which might be in the middle.

Quote:

Levster said:
I've used this tek before, it was good. I did it again, but for some reason all the mini round lids that were in the PC do NOT tighten properly anymore. the heat must have warped them



I am not sure if heating to high temps enlarges or shrinks the plastic, might depend on shape, I know my syringe plungers go looser over time, so not sure if the plunger gets wider or the black rubber is shrinking. Anyway it might be an experiment to try and overheat one of the items, i.e. the lid or the base. If the lid shrinks in high heat then it might snap onto the base better again. I supposed you could put a lid and base into the PC at the same time but not connected and heat at high heat, then test both out with your current base & lids and see if it does make a difference. Then you could heat the bulk of just your lids/bases purposely altering them to fit the bases/lids

I have been using silicone grease on lids, not just for agar. The grease is like vaseline and does not dissolve or go runny at high temps, like silicone caulk/sealant but it never sets. If it is on the rim and base container then it will stick itself together and form a seal, and the caps will not easily fall off it touched against. As it is sticky I also believe it will help trap any contaminants, e.g. if you use unmodified lids on jars and just leave them loosely on then if any dust/spores try and get up under the lid it will hopefully have a harder time making it past the sticky surface. So I smear it onto the threads of glass jars and inside the cap at the sides. It would be an interesting experiment to get a few similar jars with loose lids and blow some spores around them, this need not be contaminant spores, you could just use spare shroom spores and just not bother to take much care collecting them (rather than purposely growing mold to get contaminant spores!). Now you can see if the non greased jars are more prone to contaminate.

The scientist John Tyndall use to smear the walls of what was similar to a SAB with gylcerine to capture dust particles. But gylcerine should all liquefy and fall off it heated to our temps, as would vaseline. The best silicone grease I have used is dow molykote 111 but cheaper ones should be OK, the 111 is nice and thick.

I have not tried it yet but the grease could be good enough to make a lid for a container you have which has no lid. I have gotten containers with PP bases but PC lids, so the lids are useless with heat, I have plenty of sheet PP available so these could be cut into lid shapes and smeared with grease. Of course some will just say just go buy them but some might be in countries where things are difficult to find, or do not like wastage.


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OfflinePupMarvel
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Registered: 02/17/19
Posts: 38
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Re: Pasty Agar Tek [Re: blackout]
    #26154322 - 08/26/19 08:25 PM (4 years, 5 months ago)

Agar seemed like a big terrifing monster, thanks to this tek i just poured my first plates. I'll likely pressure cook them closer to when I need them as i'm currently dealing with an AWOL spore vendor i wont mention.


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OfflinePupMarvel
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Registered: 02/17/19
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Re: Pasty Agar Tek [Re: PupMarvel]
    #26154594 - 08/26/19 11:36 PM (4 years, 5 months ago)

OK, so I lied I was putting off the pressure cooking because it scared me. They are in there now doing their thing and I'm rather proud of myself. Going to let them cool in there over night then store them the fridge until either my spore syringes arrive or I stumble upon a tasty looking Pleurotus ostreatus I want to try and clean up.


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InvisibleHobbit GDF
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Registered: 02/14/19
Posts: 3,386
Loc: Terrapin station Flag
Re: Pasty Agar Tek [Re: PupMarvel]
    #26154779 - 08/27/19 06:32 AM (4 years, 5 months ago)

Trick with PC is once it starts it's 15psi whistle then don't forget to turn it down to medium heat (6 or 7). If you don't the PC will swell up and bow out. Now that was scary. Ruined it. Returned it. They exchanged it. It was half of so I got a dehydrator with it.
Point of story..
Make sure you lower heat once it's where you want it


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Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
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Re: Pasty Agar Tek [Re: Hobbit GDF]
    #26154782 - 08/27/19 06:37 AM (4 years, 5 months ago)

what kind of shit PC was that?  cus I know many many who use their presto PC at 20psi while running grains for over 2hrs with zero issues of swelling etc.. that would have to be a very cheap thin aluminum PC to warp like that.  :smirk:


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InvisibleHobbit GDF
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Re: Pasty Agar Tek [Re: 13shrooms]
    #26155054 - 08/27/19 10:21 AM (4 years, 5 months ago)

Presto 23qt


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InvisiblemushboyMDiscord
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Re: Pasty Agar Tek [Re: Hobbit GDF]
    #26155063 - 08/27/19 10:28 AM (4 years, 5 months ago)

that happens when you run the pc dry.


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OfflineFunnyFungiName
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Registered: 01/15/18
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Re: Pasty Agar Tek [Re: mushboy]
    #26159101 - 08/29/19 01:27 PM (4 years, 4 months ago)


It is fun watching mycelium eat jello, looks so happy.

I've been having solid results using widemouth half pints with polyfil lids. 21 fit in the pc at once. To make enough agar for 21 jars I mix 370 ml water, 5.5g lme, and 5.5g agar.


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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Pasty Agar Tek [Re: FunnyFungiName]
    #26159115 - 08/29/19 01:37 PM (4 years, 4 months ago)

That sounds really rich. I use just slightly more than that for 500ml


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OfflineDenver Tripping
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Registered: 06/27/19
Posts: 3
Last seen: 3 years, 9 months
Re: Pasty Agar Tek [Re: tryptkaloids]
    #26161998 - 08/31/19 08:30 AM (4 years, 4 months ago)

I apologize if these questions have already addressed in this thread, I've tried searching it for my questions but struggling with my android. This is my first attempt with agar and I want to get it right.

Can the mini rounds be double stacked in the pc?

I know you stated to spoon the agar into the mini rounds, but how much is needed?

Thanks!


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InvisiblePsicomb
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Registered: 01/13/18
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Re: Pasty Agar Tek [Re: Denver Tripping] * 1
    #26162048 - 08/31/19 09:21 AM (4 years, 4 months ago)

Yes they can be double stacked, even triple or more. 

Just use enough agar mix to cover the bottom of the plate entirely and leave an even surface (the bottom has indents that collect more agar so make sure you pour in enough so it's all level if that makes sense)


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OfflineDenver Tripping
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Re: Pasty Agar Tek [Re: Psicomb]
    #26162178 - 08/31/19 10:43 AM (4 years, 4 months ago)

Thanks so much!


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Offlinebulbstoo
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Re: Pasty Agar Tek [Re: Denver Tripping]
    #26162207 - 08/31/19 11:03 AM (4 years, 4 months ago)

Gotta be honest I miss my pasty plates! Lol. I switched to petris a while ago but as I look around at the environment burning to bits, I embrace all glass modes of culturing lol. Cheers


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InvisibleYogiBear
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Registered: 08/24/19
Posts: 845
Re: Pasty Agar Tek [Re: bulbstoo]
    #26170292 - 09/05/19 09:30 AM (4 years, 4 months ago)

Thank you Pasty for this Tek.

I was recommended to this Tek to get started with my first agar work. I do like the ease of this “no pour” over pouring petris... just seems like you would have less chances of getting Contams. Seems like a no brainer to this rookie.

Here are the containers I want to use instead of the mini-rounds. I like these because they are wider and are clear so the myc will be easier to see. I will prep the lids the same way. Are these the right plastic?



(((20-25 grams of agar to 1 liter of water)))
Your YouTube video has “telephone agar” so I’m going to use that instead of the bars.



(((Add 2 teaspoons (10 ml) of potato flakes)))
(I don’t want to use potato flakes... how much light malt extract should I use to 1 liter of water?)



(((Couple drops of honey)))
Honey... how much for a liter of water? Maybe a teaspoon?



Do I need any more ingredients or will the LME and honey be enough?

(((Pressure cook for 45mins. Done.)))
Got it.

Thanks in advance for any and all help.


Edited by YogiBear (09/05/19 11:51 AM)


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InvisibleYogiBear
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Registered: 08/24/19
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Re: Pasty Agar Tek [Re: YogiBear]
    #26171036 - 09/05/19 04:15 PM (4 years, 4 months ago)

Ok. I found this link so I’m going to assume that my new 8oz containers are going to work.

https://www.shroomery.org/forums/showflat.php/Number/8022499


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