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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Doc9151
Mycologist
Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 8 months
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You only need to PC agar for a max of 30 min. You carmelized the agar, it should be ok, but do agar separate from your grains.
-------------------- Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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banishedfromtime
void cult adept
Registered: 06/12/18
Posts: 46
Last seen: 2 months, 12 days
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Re: Pasty Agar Tek [Re: Doc9151]
#25962841 - 04/29/19 03:04 PM (4 years, 10 months ago) |
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Quote:
Doc9151 said: You only need to PC agar for a max of 30 min. You carmelized the agar, it should be ok, but do agar separate from your grains.
ahh okay, makes sense. will do for shorter period next time.
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MadHatter333
We Are All Mad Here
Registered: 09/20/17
Posts: 4,650
Loc: Your Momβs Rabbit Hole
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Pasty Plates FTW!
-------------------- TEKs I Like
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Sundaypro
Stranger
Registered: 12/02/18
Posts: 10
Last seen: 3 years, 27 days
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I still have to learn so much.
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storeman125
Stranger
Registered: 05/20/19
Posts: 1
Last seen: 4 years, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#26004468 - 05/20/19 10:19 PM (4 years, 10 months ago) |
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Any idea what I should be looking for when it comes to contams? And if I see any what would be the appropriate course of action?
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jay.ach
Traveler
Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 1 month, 28 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#26013338 - 05/25/19 08:30 PM (4 years, 9 months ago) |
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I am trying this one next.
-------------------- βOne's destination is never a place, but a new way of seeing things.β - Henry Miller LAGM v. 2.023 LAGM2021
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banishedfromtime
void cult adept
Registered: 06/12/18
Posts: 46
Last seen: 2 months, 12 days
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Re: Pastywhyte's Easy Agar Tek [Re: jay.ach]
#26019272 - 05/28/19 08:05 PM (4 years, 9 months ago) |
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alright I did a run of MEA (agar powder) and green food coloring.
a few questions
is it common to see a lot of white "fuzz" on top because there's more room to expand in minirounds compared to regular plates? I'm also wondering if this yellowing is the myc mixing with the tan-ish powder of the malt extract and agar, as this is happening to most of my dishes. I was curious and opened one up and dragged all of the fuzz, it collected a deep blue and when I was breaking it up I definitely caught a whiff of myc.
here are some pics of what I'm talking about
suspect fuzz apparent
can see the yellowing I'm referring to in the middle there
but behind good light this looks pretty healthy. thoughts?
edit
man uploading really degrades quality of photos. oh well.
that's probably my cleanest "plate". all from MS. if I can confirm this I'll use to kickstart some g2g jars
how long should I let the plates grow for?
Edited by banishedfromtime (05/28/19 08:08 PM)
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Mycelial Messenger
Stranger
Registered: 10/29/18
Posts: 10
Last seen: 1 year, 10 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#26031406 - 06/03/19 10:40 PM (4 years, 9 months ago) |
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Has never failed, works every time.
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Hobbit GDF
Deadhead
Registered: 02/14/19
Posts: 3,443
Loc: Terrapin station
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Is this ok? The food coloring turned from blue to yellow in a week. I accidentally squirted to much. The needle was clogged. It's Ms PE. I let it roll around in the jar too to spread it. Is it myc or trich?
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Psicomb
shroom or die
Registered: 01/13/18
Posts: 4,749
Loc: the womb
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Re: Pastywhyte's Easy Agar Tek [Re: Hobbit GDF]
#26031929 - 06/04/19 07:37 AM (4 years, 9 months ago) |
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It is probably a mixture of mold and cubensis mycelium if you havent made any transfers yet. Make a few transfers from the healthiest looking spots unless the entire plate is mold. Transfer sooner next time so you can catch any irregular growth quickly before it meshes with the myc
-------------------- When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
Edited by Psicomb (06/04/19 07:38 AM)
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Hobbit GDF
Deadhead
Registered: 02/14/19
Posts: 3,443
Loc: Terrapin station
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Re: Pastywhyte's Easy Agar Tek [Re: Psicomb]
#26032197 - 06/04/19 10:51 AM (4 years, 9 months ago) |
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I done transfers yesterday. I made two plates when I done the Ms to agar. They both turned out like this. So hopefully I didn't just transfer mold. I will post pics of the transfers in a couple days.
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10kVisions
High, Real High.
Registered: 04/08/18
Posts: 188
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pardon me if this is a stupid question but you say you use "2 Heaping Tablespoons" to get the 2.5(isn) grams of agar you need for this recipe but then go on to mention that if you want to weigh it out 1 TSP is 2.5 grams. am i missing something or are those measurements off?
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,830
Loc: Canada
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Quote:
10kVisions said: pardon me if this is a stupid question but you say you use "2 Heaping Tablespoons" to get the 2.5(isn) grams of agar you need for this recipe but then go on to mention that if you want to weigh it out 1 TSP is 2.5 grams. am i missing something or are those measurements off?
Heaping tablespoons is if youβre grinding agar bars in a coffee grinder and 1 tsp is for powdered agar. I should maybe revise that section later.
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yojay50
always learning
Registered: 04/21/17
Posts: 342
Last seen: 3 months, 29 days
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i was legit going to ask for clarification just now. 1tsp it is! i have the telephone brand. pasty ur a fuckin genius
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BlackSwanEvent
Stranger
Registered: 05/30/19
Posts: 19
Last seen: 4 years, 8 months
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Re: Pasty Agar Tek [Re: yojay50]
#26046159 - 06/11/19 03:25 PM (4 years, 9 months ago) |
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Hey guys. I didn't want to make an entire thread for this but is it okay if the micropore tape is wet when inoculated or when ran through the pressure cooker?
I did my first three plates using a multspore syringe with small ball jars following this tek and was wondering if i should be conscious of consequences.
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Hobbit GDF
Deadhead
Registered: 02/14/19
Posts: 3,443
Loc: Terrapin station
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My mp tape is wet when it's time for me to use the plates. Plus I keep getting condensation. Like alot
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JHOVA
Post whore
Registered: 02/17/17
Posts: 4,727
Loc:
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Use a paper towel and foil. Use 3 lids to elevate the trivet.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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BlackSwanEvent
Stranger
Registered: 05/30/19
Posts: 19
Last seen: 4 years, 8 months
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Cool. Any contamination problems?
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BlackSwanEvent
Stranger
Registered: 05/30/19
Posts: 19
Last seen: 4 years, 8 months
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Re: Pasty Agar Tek [Re: JHOVA]
#26046212 - 06/11/19 03:57 PM (4 years, 9 months ago) |
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Quote:
JHOVA said: Use a paper towel and foil. Use 3 lids to elevate the trivet.
So you are saying it is best to elevate them as high as possible? Mine didn't have a trivet but I made one by drilling holes into a small pan and removing the plastic handle.
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JHOVA
Post whore
Registered: 02/17/17
Posts: 4,727
Loc:
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Buy a trivet
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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