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Subfinder
Wildlife enthusiast


Registered: 07/01/18
Posts: 1,025
Loc: Pennsylvania
Last seen: 9 months, 18 days
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Re: Pastywhyte's Easy Agar Tek [Re: styro]
#25701699 - 12/25/18 07:33 AM (5 years, 1 month ago) |
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Quote:
styro said: Thanks tryp. Will see if I can find the paper kind.
Walmart has it.
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Appelmoesje
lurkert


Registered: 03/24/18
Posts: 2
Last seen: 2 years, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: Subfinder]
#25702206 - 12/25/18 02:19 PM (5 years, 1 month ago) |
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Super tek, so much thanks Pasty(et al.) This got me motivated into the hobby again and start with agar before brf-cake or grain. So i got to page 28 in 2 days, nice read. And figured i checked how many more to go...315pages.. come on. i like a thorough read, but fuk this reading i'm gonna make agar now.
Got started with petri's, LC and grain all at once. But failed miserably a few times and walked away from this hobby.(back to lady-plants) Time to clean the room and equipment, got some dirty growth since i walked out of there 8 months ago.
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Re: Pastywhyte's Easy Agar Tek [Re: Appelmoesje]
#25711512 - 12/30/18 12:02 PM (5 years, 1 month ago) |
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Awesome tek Pasty, thanks for the contribution to the community. Definitely made me more comfortable getting into agar. I've seen some discussion about other containers, and just thought I would propose these:
https://www.amazon.com/gp/product/B01HBTA20U
they're $14 for a set of 48 or about $0.30 / container. Compared to the mini-rounds which if you can get for $3.50 a pack are $0.44 / container. They're polypropylene, microwave-safe (i.e. thick enough not to warp immediately), and have clear sides and lids. They're twice the volume but significantly shorter, and the diameter is around 4" at the bottom, about the same as a 100mm petri dish, not sure if anyone uses that size.
Not sure if anyone has suggested this type of container already, I did some searches without finding anything.
Thoughts? Anyone with more experience think they're worth a shot? For some reason I've been having trouble finding the mini-rounds.
Thanks in advance!
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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Al Bundy
No Ma'am


Registered: 05/01/15
Posts: 178
Loc: Chicago
Last seen: 2 years, 8 months
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Re: Pastywhyte's Easy Agar Tek [Re: juniperus] 2
#25711518 - 12/30/18 12:06 PM (5 years, 1 month ago) |
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Those containers donβt work. The lids arenβt pp5 and they melt.
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Re: Pastywhyte's Easy Agar Tek [Re: Al Bundy]
#25711553 - 12/30/18 12:30 PM (5 years, 1 month ago) |
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Good call, upon further inspection the lids are indeed LDPE4. Damn, found some with PP5 lids but they're not affordable. Mini-rounds it is.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Pastywhyte's Easy Agar Tek [Re: juniperus]
#25711612 - 12/30/18 01:28 PM (5 years, 30 days ago) |
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where's the line for affordable?
i've been using 75mm pp5 perti dishes. about $0.69 each. seem to be holding up pretty well. also, i just discovered a suction cup stuck to the bottom plate makes for incredibly smooth handling when doing transfers and inoculations. my favorite feature. but they are legit petri dishes, so they gotta be wrapped, and i can't figure out how to keep them dry inside. [hashtag]tradeoffs
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the j stands for jesus.2020 new years grow along
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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That's pretty affordable, in my book. But those need to be poured, correct? Or are you using them with this tek, i.e. no-pour? Do you use plastic wrap to wrap them?
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Pastywhyte's Easy Agar Tek [Re: juniperus]
#25711658 - 12/30/18 02:11 PM (5 years, 30 days ago) |
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i may try pouring at some point, but for now i'm no-pouring all the way. honestly, i don't know how i would go about sterilizing them to pour them. glass ones can be baked. i doubt the plastic ones would be so good in the oven.
i've tried plastic wrap and parafilm. i prefer parafilm right now, but i'm not committed.
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the j stands for jesus.2020 new years grow along
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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I believe you are sterilizing them in the PC with the no-pour already. So I guess you would just do a similar process but with the empty dishes. Not sure though, don't have any tangible experience but think I understand the concept of sterilization vs sanitation etc. Have you had any issues due to the condensation, like spores/contams being spread throughout the plate?
Personally, I'll probably just pick up some mini-rounds. Seems pretty cost effective and no need to deviate from the tek unless there's a good reason.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Re: Pastywhyte's Easy Agar Tek [Re: juniperus]
#25711775 - 12/30/18 03:24 PM (5 years, 30 days ago) |
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yeah man, find what works for you. rule number three.
i have not had undue contamination issues. i'm thinking of modifying lids like the pastyplate to help with excess moisture. eye dee kay. i really love that suction cup thing, tho, so it's worth its downsides to me.
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the j stands for jesus.2020 new years grow along
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 1 hour
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Most plastic dishes cant be reported. If they're pp5 make foil sleeves and pc
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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i wrap stacks of nine together in foil and pc for 30 minutes. i've made several approaches to drying them out, most of which have failed totally. the moisture is irksome, but not a deal breaker for me. maybe i should give the paper towel squares a try.
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the j stands for jesus.2020 new years grow along
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Prince0fThieves
Neophyte



Registered: 08/24/17
Posts: 27
Loc: ican
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Juniperus, I use very similar containers and they work great. Make sure to make a bigger agar batch, the bottoms are wider.
The exact brand and model number:
Karat fp-dcl117-pp
The lids are also pp5.
I did the side hole with medical tape (1 layer), and i PC for 40min.
They work well. I got tied up after making a batch and decided to do an experiment on how long it would take to contam. Out of 6 in a closet and 10 in my fridge, I only had 1 get the tiniest spot of mold after 4 months. (Probably didn't get bigger because the plates had pretty well dehydrated by then in the area nearest the hole)
Some moisture is normal on the lid and sides, but I've found that if you let it get on you agar that it can mess with a good culture.
Good luck
Edited by Prince0fThieves (12/30/18 09:19 PM)
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Prince0fThieves
Neophyte



Registered: 08/24/17
Posts: 27
Loc: ican
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I just noticed, the lids have that code on them (dcl117-pp) But the container itself says fp-dc8-pp.
They are just 8oz deli containers by Karat.
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Quote:
Prince0fThieves said: Juniperus, I use very similar containers and they work great. Make sure to make a bigger agar batch, the bottoms are wider.
The exact brand and model number:
Karat fp-dcl117-pp
The lids are also pp5.
I did the side hole with medical tape (1 layer), and i PC for 40min.
They work well. I got tied up after making a batch and decided to do an experiment on how long it would take to contam. Out of 6 in a closet and 10 in my fridge, I only had 1 get the tiniest spot of mold after 4 months. (Probably didn't get bigger because the plates had pretty well dehydrated by then in the area nearest the hole)
Some moisture is normal on the lid and sides, but I've found that if you let it get on you agar that it can mess with a good culture.
Good luck 
Thank you good sir!!
Edit: Ah! I actually just asked about something similar in another thread. I was wondering if those thinner containers would work and it seems like you answered that question. Thanks again!
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
Edited by juniperus (12/30/18 09:52 PM)
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growlingmycelium
/@_@/



Registered: 04/15/16
Posts: 346
Loc: Durkadurkastan
Last seen: 7 months, 20 days
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Re: Pastywhyte's Easy Agar Tek [Re: juniperus]
#25712977 - 12/31/18 12:46 AM (5 years, 30 days ago) |
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I'm sure this has been answerd, and I really did thumb through 100 pages to find it first before I'm asking.
Can I just keep the paper towel on and not take it off? When I do in the SAB after I take off my foil my microscope tape is always dissolved on the paper towel. Even with 2 pieces and bringing up slow.
I see that it's labeled at the bottom of the tek like pasty keeps in on I'm just making sure i'm in fact an idiot and I can keep it on -_-
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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keep the paper towel and micropore tape on the top of the pasty plate. the only thing to put on and remove is the aluminum foil.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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growlingmycelium
/@_@/



Registered: 04/15/16
Posts: 346
Loc: Durkadurkastan
Last seen: 7 months, 20 days
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Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
#25713008 - 12/31/18 01:30 AM (5 years, 30 days ago) |
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Quote:
JHOVA said: keep the paper towel and micropore tape on the top of the pasty plate. the only thing to put on and remove is the aluminum foil.
Thanks m8. Doing a run now.
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dmar



Registered: 09/09/18
Posts: 147
Loc: Earth, Milky Way, Virgo Superc...
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I swiped a spore print last week and it germinated nicely, did a transfer, and it was going good but there was an odd dot away from everything else, so I went ahead and did another transfer just in case it was trich that was still white. Well now after doing that transfer, both of the plates I transferred to have little dots all over them as well. I've never had this problem before, but I remember RR saying in his Agar video that a satellite colony is bad news. Should I wait and see what happens or carefully do another transfer to try and get away from whatever it is?
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18 
Posts: 4,843
Loc: broom closet
Last seen: 7 days, 3 hours
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Re: Pastywhyte's Easy Agar Tek [Re: dmar]
#25719135 - 01/02/19 03:45 PM (5 years, 27 days ago) |
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Unless you "double dribbled" those transfers I'd say your sterile technique needs work. You don't really have much growth you can transfer yet though. How do your untouched dishes look?
-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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