Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Bridgetown Botanicals CBD Concentrates

Jump to first unread post Pages: < First | < Back | 332 | 333 | 334 | 335 | 336 | 337 | 338 | 339 | 340 | 341 | 342 | 343 | 344 | 345 | 346 | 347 | 348 | 349 | 350 | 351 | 352 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinebulbstoo
Stranger
 User Gallery


Registered: 10/27/18
Posts: 120
Last seen: 4 years, 1 month
Re: Pasty Agar Tek [Re: Stu1234]
    #25665908 - 12/08/18 02:50 PM (5 years, 1 month ago)

Quote:

Stu1234 said:
OK OK I know, I could read all the way through 342 pages of this thread but I'm just gonna ask it....You have your Agar plates all ready to go, do you just cut a small piece of mushroom and place it on the plate or am I so far off here it's laughable????




Yes, you can take a very thin piece of clean tissue from inside the steam and place on agar in a still air box.


--------------------


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Pasty Agar Tek [Re: bulbstoo]
    #25666162 - 12/08/18 04:57 PM (5 years, 1 month ago)

I like to tear the fruit in half and drag the scalpel through the inner flesh to get a sample.


Extras: Filter Print Post Top
OfflineEdmunter
Mr
Male User Gallery


Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 20 hours
Re: Pasty Agar Tek [Re: Pastywhyte]
    #25666903 - 12/08/18 11:17 PM (5 years, 1 month ago)

Quote:

Pastywhyte said:
I like to tear the fruit in half and drag the scalpel through the inner flesh to get a sample.




I did an experiment to see is open air cloning was a disaster.  All I was attempting was to get the tiniest bit of Myc growth to cleanly transfer asap.  Result wa I beat all the contaminants and grabbed my culture.


Extras: Filter Print Post Top
Invisiblesilave
Theoretical Researcher
I'm a teapot
Registered: 10/12/18
Posts: 23
Re: Pasty Agar Tek [Re: Edmunter]
    #25667286 - 12/09/18 07:38 AM (5 years, 1 month ago)

Hey, new poster here.

How do you prevent condensation from building up underneath the lid? I drilled the hole on the side but I still ended up with a fair amount of water built up inside some of the mini-rounds when I took them out the PC.


--------------------
Looking to cultivate and master legal mushroom! Also interested in microscopic research on psychoactive shroom compounds.


Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pasty Agar Tek [Re: silave]
    #25667593 - 12/09/18 10:20 AM (5 years, 1 month ago)

Normally all no pour should be done container upside down 🙃 except when dumping excess water. Carefully dump any excess water in a sab. Excess water evaporates in 5 days if you do nothing.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Extras: Filter Print Post Top
Invisiblesilave
Theoretical Researcher
I'm a teapot
Registered: 10/12/18
Posts: 23
Re: Pasty Agar Tek [Re: JHOVA]
    #25673869 - 12/12/18 07:39 AM (5 years, 1 month ago)

Ty for the help JHOVA


--------------------
Looking to cultivate and master legal mushroom! Also interested in microscopic research on psychoactive shroom compounds.


Extras: Filter Print Post Top
OfflineCaster
Stranger
I'm a teapot


Registered: 04/17/18
Posts: 533
Last seen: 8 months, 7 days
Re: Pasty Agar Tek [Re: silave]
    #25675936 - 12/13/18 02:05 AM (5 years, 1 month ago)

Do we need to cook in the pressure cooker at a certain pressure? Or does it not matter? Just using the pc as a sealed steaming unit to sterilise the contents?

thanks.


Extras: Filter Print Post Top
Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Pasty Agar Tek [Re: Caster] * 1
    #25676025 - 12/13/18 04:28 AM (5 years, 1 month ago)

15-18 psi it will be sterilized at lower psi it just takes longer


Extras: Filter Print Post Top
OfflineCaster
Stranger
I'm a teapot


Registered: 04/17/18
Posts: 533
Last seen: 8 months, 7 days
Re: Pasty Agar Tek [Re: thelanzii]
    #25676031 - 12/13/18 04:43 AM (5 years, 1 month ago)

Quote:

EtherealMind said:
15-18 psi it will be sterilized at lower psi it just takes longer




Thank you very much, the reason why I ask is my pressure cooker will only reach 8 psi, so I'll just add another 30-60 mins on the time.


Edited by Caster (12/13/18 04:46 AM)


Extras: Filter Print Post Top
OfflineDoc9151M
Mycologist
Male User Gallery


Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: Pasty Agar Tek [Re: Caster]
    #25677727 - 12/13/18 07:49 PM (5 years, 1 month ago)

There's a difference in a pressure cooker and a pressure canner. Pressure cookers do not get hot enough to sterilize properly, but a pressure canner can and has been used successfully as a sterilizer and will reach the temperatures necessary to sterilize grains, surgical instruments and much more.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Extras: Filter Print Post Top
OfflineTime Tourist
curioser and curioser
Male User Gallery


Registered: 01/02/16
Posts: 101
Last seen: 1 year, 4 days
Re: Pasty Agar Tek [Re: Doc9151]
    #25678972 - 12/14/18 12:56 PM (5 years, 1 month ago)

I'm just wondering about this in general, how many plates do you guys have before beginning?
I always seem to find myself in need of more when I need to do transfers. And how many transfers should I calculate in the whole process?


Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery

Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: Pasty Agar Tek [Re: Time Tourist]
    #25679023 - 12/14/18 01:25 PM (5 years, 1 month ago)

As many transfers as it takes to see uniform clean growth.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Extras: Filter Print Post Top
OfflineCaster
Stranger
I'm a teapot


Registered: 04/17/18
Posts: 533
Last seen: 8 months, 7 days
Re: Pasty Agar Tek [Re: JHOVA]
    #25687749 - 12/18/18 04:00 PM (5 years, 1 month ago)

Some agar I have made has a thin layer of liquid on the top, I have a feeling that something happened when I was cooling them down and some humidity got in there and made some water. Does this mean this batch is ruined? Or can I pour this excess liquid off of the top and itll still be ok?

I put some droplets of spore syringe in there before I realised that there was excess liquid, will that extra liquid cause it to not grow anything? It's been about 3 days and I can't see anything at all in there.

I think next time I might use something to scrape the spores off the foil with instead of using a spore syringe. Would a qtip suffice? or i've also made a very dodgy inoculation loop. That might be better.


Thanks all :smile:


Extras: Filter Print Post Top
InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Pasty Agar Tek [Re: Caster] * 1
    #25688267 - 12/18/18 08:33 PM (5 years, 1 month ago)

when you go to inoculate the dish, turn the plate over first to let the moisture drip off. what remains should evaporate through the tape. i store my damp plates upside down.

spores can take a while to germinate. not sure if the water will prevent this, but i reckon it will be ok. again, consider storing upside down.

bodhisatta made a sweet how to make a noc loop thread. find it.
swabs work, if you sterilize them first. wrapped in foil, in the PC for a little while.


Extras: Filter Print Post Top
OfflineCaster
Stranger
I'm a teapot


Registered: 04/17/18
Posts: 533
Last seen: 8 months, 7 days
Re: Pasty Agar Tek [Re: J. Jack Flash]
    #25688291 - 12/18/18 08:51 PM (5 years, 1 month ago)

Quote:

J. Jack Flash said:
when you go to inoculate the dish, turn the plate over first to let the moisture drip off. what remains should evaporate through the tape. i store my damp plates upside down.

spores can take a while to germinate. not sure if the water will prevent this, but i reckon it will be ok. again, consider storing upside down.

bodhisatta made a sweet how to make a noc loop thread. find it.
swabs work, if you sterilize them first. wrapped in foil, in the PC for a little while.





Thank you for the help and the tips! I'll know for next time now, I'll go and find that tutorial too now :smile:


Extras: Filter Print Post Top
Offlinestyro
Stranger

Registered: 05/30/18
Posts: 53
Last seen: 4 years, 9 months
Re: Pasty Agar Tek *DELETED* [Re: Caster]
    #25698863 - 12/23/18 07:03 PM (5 years, 1 month ago)

Post deleted by styro

Reason for deletion: ****


Extras: Filter Print Post Top
InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Pasty Agar Tek [Re: styro]
    #25698918 - 12/23/18 07:35 PM (5 years, 1 month ago)

do it man, you got this. :thumbup:


Extras: Filter Print Post Top
Offlinestyro
Stranger

Registered: 05/30/18
Posts: 53
Last seen: 4 years, 9 months
Re: Pastywhyte's Easy Agar Tek *DELETED* [Re: Pastywhyte]
    #25701014 - 12/24/18 08:11 PM (5 years, 1 month ago)

Post deleted by styro

Reason for deletion: ****


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 47 minutes
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: styro]
    #25701179 - 12/24/18 09:55 PM (5 years, 1 month ago)

You want "paper tape" and yes lids get a little loose. Its a good thing


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Offlinestyro
Stranger

Registered: 05/30/18
Posts: 53
Last seen: 4 years, 9 months
Re: Pastywhyte's Easy Agar Tek *DELETED* [Re: tryptkaloids]
    #25701695 - 12/25/18 07:31 AM (5 years, 1 month ago)

Post deleted by styro

Reason for deletion: ****


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 332 | 333 | 334 | 335 | 336 | 337 | 338 | 339 | 340 | 341 | 342 | 343 | 344 | 345 | 346 | 347 | 348 | 349 | 350 | 351 | 352 | Next > | Last >

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Bridgetown Botanicals CBD Concentrates


Similar ThreadsPosterViewsRepliesLast post
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,732 8 12/13/19 06:16 PM
by mushboy
* Agar Tek Aristar 5,815 12 08/01/03 06:42 PM
by shirley knott
* Easy syringe tek? pleasesmokemarijuana 2,357 8 04/02/03 07:09 PM
by Roadkill
* Easy Poo Tek Audi0 3,429 8 12/15/02 09:04 AM
by PDU
* Re: Peroxidated Agar Tek sparks 4,555 6 05/08/00 12:43 AM
by Anonymous
* easy agar source? KafkaPie 1,092 1 05/13/03 11:43 PM
by micro
* Easy Syringe Tek? Tenchi 2,789 18 01/28/03 05:06 AM
by Azmodeus
* agar tek? the man 2,773 4 12/16/02 10:45 AM
by the man

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
794,008 topic views. 40 members, 225 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.037 seconds spending 0.016 seconds on 15 queries.