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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: JHOVA]
    #25563613 - 10/24/18 02:28 PM (5 years, 3 months ago)

Im not saying it cant be done, just that this is clearly a phone over a plate.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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OfflineBignate316
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25563809 - 10/24/18 03:23 PM (5 years, 3 months ago)

Yeah the phone was about 6-8 in. above it.
So is the general consensus bacteria or should I wait and see?


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Bignate316]
    #25563816 - 10/24/18 03:24 PM (5 years, 3 months ago)

No, its absolutely, 100%  a bacterial colony


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineCaster
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #25563817 - 10/24/18 03:24 PM (5 years, 3 months ago)

Quote:

Pastywhyte said:
If you want to use potatoes just dice a bunch up, boil em, save the water and eat the taters. Then use the water.



That's a nice and easy option, just replace what I would use the potato flakes with potato water? Same weight /amount? :smile:

Thanks


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OfflineBignate316
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25563858 - 10/24/18 03:36 PM (5 years, 3 months ago)

Quote:

tryptkaloids said:
No, its absolutely, 100%  a bacterial colony




Thanks tryp I'll toss em outside when I get home from work. I'll probably toss the jars and redo them this weekend as well since all of the plates went bacterial right where the spore solution dropped there's no way anything good is in those jars.


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: Bignate316]
    #25564089 - 10/24/18 04:45 PM (5 years, 3 months ago)



Like this is what i meant


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OfflineBignate316
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Re: Pasty Agar Tek [Re: JHOVA]
    #25564109 - 10/24/18 04:53 PM (5 years, 3 months ago)

Ahh that makes perfect sense. I didn't want the lid off for too long so I popped it off with one hand and snapped the photo with the other real quick.
But I think I like your way better, don't even put the phone in the SAB.


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: Bignate316]
    #25564443 - 10/24/18 07:00 PM (5 years, 3 months ago)

This is the way you use pasty plates. On their side or upside down.


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OfflineRomeoPapa
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Re: Pasty Agar Tek [Re: JHOVA]
    #25564634 - 10/24/18 08:29 PM (5 years, 3 months ago)

Pasty plate with 1% agar powder and 1% malt per volume.

This is Rustywhyte culture as well. I think it's ready to chew through a grain jar soon.



They are so hard to take a good picture of. I believe this is a clean culture though!


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It's better to have it and not need it
Than it is to need it and not have it.



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OfflineBignate316
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Re: Pasty Agar Tek [Re: RomeoPapa]
    #25564756 - 10/24/18 09:15 PM (5 years, 3 months ago)

What if you put a clean piece of saran wrap over the top before putting the lid on? Or is Saran wrap not sterile?


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OfflineRomeoPapa
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Re: Pasty Agar Tek [Re: Bignate316]
    #25564777 - 10/24/18 09:19 PM (5 years, 3 months ago)

It might work. Probably not though. It's a vector for contamination that I would want to avoid. I'm not in this for a photo OP! Lol.


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It's better to have it and not need it
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OfflineBignate316
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Re: Pasty Agar Tek [Re: RomeoPapa]
    #25564879 - 10/24/18 10:09 PM (5 years, 3 months ago)

Quote:

RomeoPapa said:
It might work. Probably not though. It's a vector for contamination that I would want to avoid. I'm not in this for a photo OP! Lol.




I meant for your own convenience to be able to see it better. But I suppose you're right, I mean imagine trying to tear off a piece of saran wrap inside a sab. Lol


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Bignate316]
    #25565180 - 10/25/18 01:08 AM (5 years, 3 months ago)

It wouldn't be convenient. When I first got started, I drilled a small hole in the top of my SAB about the size of my phone camera lens. I'd line it up, open the PP for a second, and snap a couple pics. Don't recall things contaminating from that, but it's unnecessary when you get better at spotting contams. You can just eyeball it through the plastic and make a final decision before transferring.


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OfflineBignate316
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Re: Pasty Agar Tek [Re: stareatclouds]
    #25565204 - 10/25/18 01:34 AM (5 years, 3 months ago)

Quote:

stareatclouds said:
It wouldn't be convenient. When I first got started, I drilled a small hole in the top of my SAB about the size of my phone camera lens. I'd line it up, open the PP for a second, and snap a couple pics. Don't recall things contaminating from that, but it's unnecessary when you get better at spotting contams. You can just eyeball it through the plastic and make a final decision before transferring.




I would think having an extra hole in the top of my sab JUST for taking pictures would be incredibly inconvenient and cause lots of contams.
But what do I know I'm just a dumb noob who doesn't listen to anyone's advice :wink:


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Bignate316]
    #25565369 - 10/25/18 03:54 AM (5 years, 3 months ago)

Yes, the hole gets covered with tape when you're not taking pictures. Most totes are probably clear enough to zoom through the plastic though.


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OfflineBignate316
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Re: Pasty Agar Tek [Re: stareatclouds]
    #25566176 - 10/25/18 11:10 AM (5 years, 3 months ago)

I can see why they (sab's) are not clear enough to see through if you sprayed the inside with soapy water or iso before you started. It would be like trying to look through a pasty plate with the lid on. Lol


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Re: Pasty Agar Tek [Re: Bignate316]
    #25566183 - 10/25/18 11:12 AM (5 years, 3 months ago)

Spitball jedi built a sab with plexiglass in the front. You could do the same for the top.


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Offlineverum subsequentis
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Re: Pasty Agar Tek [Re: JHOVA]
    #25566239 - 10/25/18 11:30 AM (5 years, 3 months ago)

my sab has a glass window on the front. i got really tired really quickly trying to identify shit through a semi clear-foggy plastic wall.


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Re: Pasty Agar Tek [Re: verum subsequentis]
    #25566263 - 10/25/18 11:38 AM (5 years, 3 months ago)

I like these dollar store containers for pastyplates, the lids are flat and really clear-

https://www.dollartree.com/12oz-sure-fresh-small-round-plastic-storage-containers-4ct-packs/809574

Theses pics were taken through the lids.



Edit:fixed images


Edited by foragedfungus (10/25/18 11:43 AM)


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OfflineDoc9151M
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Re: Pasty Agar Tek [Re: pablokabute]
    #25566268 - 10/25/18 11:39 AM (5 years, 3 months ago)

Quote:

pablokabute said:
Quote:

Bignate316 said:
Any thoughts? All my plates just started showing signs of growth, look like this and I can't tell if that's myc or not.







did you just opened your plate to snap a pic?



This definitely bacterial, not mushroom mycelium.


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