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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25509611 - 10/03/18 03:29 PM (5 years, 3 months ago)

Whether it's powdered or bar doesn't matter, but the ingredients do. There's little packets of powdered agar at markets that have sugars added to them since people use it to eat. Make sure it's straight agar.


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OfflineFunnyFungiName
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Re: Pasty Agar Tek [Re: stareatclouds]
    #25509948 - 10/03/18 05:57 PM (5 years, 3 months ago)

I'm sure it's on one of these pages, but I can't find the solution to crushed plates. Every batch I make has 1 or 2 pastys sucked in on the bottom, any solution?


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: FunnyFungiName]
    #25510031 - 10/03/18 06:14 PM (5 years, 3 months ago)

Quote:

FunnyFungiName said:
I'm sure it's on one of these pages, but I can't find the solution to crushed plates. Every batch I make has 1 or 2 pastys sucked in on the bottom, any solution?




They usually get crushed if they pull a vacuum. Too much restriction on the GE hole is usually the problem. Did you use a smaller hole than 1/4”


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OfflineFunnyFungiName
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #25510770 - 10/04/18 12:06 AM (5 years, 3 months ago)

The hole is a bit small on some of them so it seems that was the problem. Much appeciated!!!


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OfflineCaster
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Re: Pasty Agar Tek [Re: Pastywhyte] * 1
    #25510848 - 10/04/18 02:24 AM (5 years, 3 months ago)

Quote:

Pastywhyte said:
Quote:

Caster said:
This is great! Going to try this with some oyster mushrooms.



EDIT: one little thing I am confused about - the Agar Agar which I can buy is all powdered form, does this matter?






If you read the op I mention that you can use powdered agar and I even give measurements in volume for powdered agar to help those who lack a scale. Cheers :cheers:




Thanks man, sorry I missed it, I've managed to find some agar agar without the added sugars and stuff! So that's good. Just gotta wait for it to arrive. Managed to find some containers but they're a bit bigger than normal, and also some rectangle ones. Going to give them both a go and see how it goes.

I'm excited! This should be heaps of fun, going to go collect some mycelium from somewhere and try and grow it. Need to make myself a SAB next.


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OfflineHydropimp
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Re: Pasty Agar Tek [Re: Caster] * 1
    #25515595 - 10/05/18 06:46 PM (5 years, 3 months ago)

Just wanted to come in and say thanks for the tutorial. I did my first plates there where some that had bacteria. And one lone one that grew myc. I then made more plates and just did 1plate to 9 others. They are growing good. I will then drop the tiger drop into each bag of pre sterilized rye berry bags i have .

Thanks everyone. I am on to my new passion. If you fail try again and again and again. You learn more from trial and error than gettig lucky the first go around.

Relentless

Pimp out.

Till my next step which will be to colonize the grain.

Then onto bodhis coco tek i got 6 bins to fill up for my first try go big or dont even come outside


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Hydropimp] * 1
    #25516145 - 10/05/18 11:36 PM (5 years, 3 months ago)

Quote:

Hydropimp said:
You learn more from trial and error than gettig lucky the first go around.



Fuckin' Truth.

It's really too bad some people get so salty about a little bump in the road.


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OfflineManifoldPrime
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Re: Pasty Agar Tek [Re: Hydropimp]
    #25516346 - 10/06/18 03:22 AM (5 years, 3 months ago)

I like the cut of your jib Hydropimp

God knows I've failed in this hobby more times than was strictly necessary


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OfflineHydropimp
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Re: Pasty Agar Tek [Re: ManifoldPrime]
    #25517418 - 10/06/18 02:34 PM (5 years, 3 months ago)

Sup prime

Am new to this what is jib


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Hydropimp]
    #25517759 - 10/06/18 05:16 PM (5 years, 3 months ago)

Jib jab? I think its a term along the lines of well said


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25517796 - 10/06/18 05:40 PM (5 years, 3 months ago)

Quote:

cut of one's jib
One's general appearance or personality, as in I don't like the cut of Ben's jib. In the 17th century the shape of the jib sail often identified a vessel's nationality, and hence whether it was hostile or friendly. The term was being used figuratively by the early 1800s, often to express like or dislike for someone.




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OfflineHydropimp
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Re: Pasty Agar Tek [Re: JHOVA]
    #25518542 - 10/07/18 12:24 AM (5 years, 3 months ago)

Thanks fellas keep it pimping tho


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OfflineDayglo Joe


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Re: Pastywhyte's Easy Agar Tek [Re: ManifoldPrime]
    #25518782 - 10/07/18 04:54 AM (5 years, 3 months ago)

Quote:

ManifoldPrime said:
Quote:

Dayglo Joe said:
Just poured my first sleeve!

...
It crossed my mind that maybe I should "sleeve" and "de-sleeve" in the SAB with gloves and propper sterile procedures?

Im sure thats been discussed
But I somehow missed it
And thought id mention it here.





I cut the sleeve of petries open in the SAB before I open the agar bottle. I usually do 30, which fits in my SAB snugly. I desleeve, open bottle halfway, split 10 stack into 5 stacks, open and pour bottom to top. when the agars finished, I pull the bottle out, wait 2-5 mins, re-apply alcohol gel to hands (sometimes I pour so hot my gloves get warm and dry, and cooling them down a touch helps comfort-wise) and pour the next stack.

For no Pour I just pop em in the SAB after cooking, and nocc up asap, with wedges, spore solution, or LC I'm testing.




I appreciate the tips, Mani P.

The lure of increased visibilty is strong...

But these Pastey Plates have me hooked with their user friendliness and re-usability!

I'll give petris another go later on.
But for now, the more familiar I get with these Pastys, the better my eyes get...


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OfflineDayglo Joe


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Re: Pastywhyte's Easy Agar Tek [Re: Dayglo Joe] * 1
    #25518785 - 10/07/18 05:01 AM (5 years, 3 months ago)

Also,
A good jib is useful for getting a good bite on an upwind tack...
Ahoy pastey mateys
Thar she blows!
The great white whale!


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OfflineHydropimp
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Re: Pastywhyte's Easy Agar Tek [Re: Dayglo Joe]
    #25520517 - 10/07/18 07:42 PM (5 years, 3 months ago)



The black is not bacteria its burnt agar from a hot blade


Edited by Hydropimp (10/07/18 10:38 PM)


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Offlinelifescientist
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Re: Pastywhyte's Easy Agar Tek [Re: Hydropimp]
    #25543020 - 10/16/18 07:29 PM (5 years, 3 months ago)

Can somebody tell me if they see contams on these? I am planning on transplanting on Sunday







edit: I failed at labeling so I think I put these down in August...


Edited by lifescientist (10/16/18 07:59 PM)


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OfflineBignate316
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Re: Pastywhyte's Easy Agar Tek [Re: lifescientist]
    #25544408 - 10/17/18 11:31 AM (5 years, 3 months ago)

I'm looking to get into agar very soon and have all my supplies, just need to build my SAB.
  Anyways when it comes to inoculating the agar does it matter if the agar is set up or not?


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OfflineGrimsweeper
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Re: Pastywhyte's Easy Agar Tek [Re: Bignate316]
    #25544546 - 10/17/18 12:25 PM (5 years, 3 months ago)

If it's not set up it's probably hot enough to kill whatever you're putting on it. It only takes a few minutes to set up.


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OfflineBignate316
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Re: Pastywhyte's Easy Agar Tek [Re: Grimsweeper]
    #25544624 - 10/17/18 12:58 PM (5 years, 3 months ago)

Quote:

Grimsweeper said:
If it's not set up it's probably hot enough to kill whatever you're putting on it. It only takes a few minutes to set up.




Thanks! That makes perfect sense. I wasn't sure if it took a while like jello or if it needed to be refrigerated prior to inoculation.


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Offlineverum subsequentis
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Re: Pastywhyte's Easy Agar Tek [Re: lifescientist]
    #25547501 - 10/18/18 01:41 PM (5 years, 3 months ago)

agar will setup really quickly when in small amounts like plates.

Quote:

lifescientist said:
Can somebody tell me if they see contams on these? I am planning on transplanting on Sunday







edit: I failed at labeling so I think I put these down in August...




it's really hard to tell from those pictures. pasty plates are hard for me to see in even in real life. if you are unsure the best thing you can do is make another transfer and see what grows. you could even take a picture of it with the lid off (after you transfer) and give us a better look


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