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Offlinelifescientist
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Registered: 10/31/17
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Re: Pasty Agar Tek [Re: Legend21]
    #25343727 - 07/24/18 05:40 AM (5 years, 6 months ago)

Thanks. Sorry about the sponsor thing. I guess I'll do another and hopefully it works better this time. Damn I hope I have some left lol otherwise I'll have to get another syringe I guess


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OfflineManifoldPrime
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Re: Pasty Agar Tek [Re: lifescientist]
    #25344335 - 07/24/18 12:39 PM (5 years, 6 months ago)

Dude 30 hours is not a long period of time at all by the way, people in the RustyWhyte thread were stoked that someone got germination of spores on plates in 2 days (so 48 hours). It can take a week, longer if the spores are old/dehydrated.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: ManifoldPrime] * 1
    #25344493 - 07/24/18 02:07 PM (5 years, 6 months ago)

Yeah 48 hours is almost unheard of. Like record breaking. If I had germination in 30 hours I would suspect it was mold.


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Offlinemagicmush17
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #25345980 - 07/25/18 10:52 AM (5 years, 6 months ago)

Followed the instructions but after pressure cooking had a few glad containers get crushed and lids are loose now.  They have holes don’t know why some got crushed. I did snap down the lids for pressure cooking. Was I supposed to leave the lids on loose when pressure cooking? And should I wrap the loose lids?


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: magicmush17]
    #25346023 - 07/25/18 11:16 AM (5 years, 6 months ago)

Re read the tek. You need micropore tape and a hole.


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OfflineLegend21
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Re: Pasty Agar Tek [Re: magicmush17]
    #25346029 - 07/25/18 11:17 AM (5 years, 6 months ago)

Quote:

JHOVA said:
Re read the tek. You need micropore tape and a hole.



Hey said they have holes :shrug:


Quote:

magicmush17 said:
Followed the instructions but after pressure cooking had a few glad containers get crushed and lids are loose now.  They have holes don’t know why some got crushed. I did snap down the lids for pressure cooking. Was I supposed to leave the lids on loose when pressure cooking? And should I wrap the loose lids?



I’ve never had any crush before. How many did you put in at once time and how long did you PC them?


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: Legend21]
    #25346038 - 07/25/18 11:20 AM (5 years, 6 months ago)

Upload a pic of your pasty plate. Either the hole is too small or clogged. Or user error in dropping pressure by removing the weight.


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Offlineverum subsequentis
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Re: Pasty Agar Tek [Re: JHOVA]
    #25346267 - 07/25/18 01:40 PM (5 years, 6 months ago)

or you had heavy shit stacked on top of them


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OfflineGinge
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Re: Pasty Agar Tek [Re: verum subsequentis]
    #25347609 - 07/26/18 08:03 AM (5 years, 6 months ago)

I made a labeling mistake! Argh!

I have two ziplocs of pasty plates, one has been PC'd one has not, in the fridge.  I didn't label because I had 7 pc'd and 6 not, easy to tell apart.  Then I had a random contam and had to transfer, grabbed a PC'd plate and transfered.

Now I need/want to do some transfers and I do not know which is which.  My question is, is there anything wrong with PCing a pasty plate twice to ensure all of my plates have been PC'd?

Also, how long do you leave a plate and wait for MS inoculation?  I noc'd 16 plates with treasure coast spores from a vendor July 15.  8 of them have already been transferred, 8 of them show nothing.


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Offlinek5hd2y
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Re: Pasty Agar Tek [Re: Ginge]
    #25347627 - 07/26/18 08:19 AM (5 years, 6 months ago)

yes you can PC them twice no problem man!

could take 4-5 days to see growth and up to a couple weeks. if sooner than 3 days maybe contams so watch close.


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OfflineGinge
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Re: Pasty Agar Tek [Re: k5hd2y]
    #25347736 - 07/26/18 09:27 AM (5 years, 6 months ago)

Awesome, thanks for the quick reply, pressurizing the cooker now.

Yeah, these have been going for 11 days, I guess I will leave them until 30, unless I need the plates for viable, growing myc.  I must have been really lucky with my GT on PDA, I had strong enough growth to transfer in 5 days!
MS noc'd 7/15, transferred 7/20, and how one of the second transfers is looking today.  Ps these containers suck, I am sorry.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Ginge]
    #25348300 - 07/26/18 02:42 PM (5 years, 6 months ago)

Quote:

Ginge said: My question is, is there anything wrong with PCing a pasty plate twice to ensure all of my plates have been PC'd?


only that doing it twice wastes time and energy. But so does growing mold so I would do it.
Quote:

Ginge said:
Also, how long do you leave a plate and wait for MS inoculation?  I noc'd 16 plates with treasure coast spores from a vendor July 15.  8 of them have already been transferred, 8 of them show nothing.



Until they germinate or the other ones need a transfer. There have been a few times that inoculate 8 plates, 3 germinate then they need transfers but im too lazy to cook more agar so i just transfer to the plates inoculated on the same day


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemisfit78
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25350959 - 07/27/18 06:31 PM (5 years, 6 months ago)

What psi for the 45 minute


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: misfit78]
    #25350970 - 07/27/18 06:37 PM (5 years, 6 months ago)

15 like everything in this hobby


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Offlinemisfit78
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Re: Pasty Agar Tek [Re: JHOVA]
    #25350984 - 07/27/18 06:40 PM (5 years, 6 months ago)

Quote:

JHOVA said:
15 like everything in this hobby



:borat:


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Offlinemagikarp
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Registered: 07/17/18
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Re: Pasty Agar Tek [Re: misfit78]
    #25351182 - 07/27/18 08:20 PM (5 years, 6 months ago)

hey guys, day 5 of my pastys here, how are they looking? are the black specs on the underside harmless? search bar says they might just be spores but i dunno... ill be making grain water agar when im ready to transfer my isolates once i have enough growth for ~10 clean clones. the condensation makes it hard to get a good pic, sorry bout that







Edited by magikarp (07/27/18 08:22 PM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: magikarp]
    #25351419 - 07/27/18 11:03 PM (5 years, 6 months ago)

Those specs are undissolved potato flakes


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineicetech
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #25351786 - 07/28/18 07:42 AM (5 years, 6 months ago)

Quote:

tryptkaloids said:
Those specs are undissolved potato flakes




Which is why potato flakes are the devil.. go brf:)


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Offlinemagikarp
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Re: Pasty Agar Tek [Re: icetech]
    #25351921 - 07/28/18 09:18 AM (5 years, 6 months ago)

sorry i should have clarified, i used malt extract not tater flakes! ill be using grain water for my 2nd round isolates.


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: magikarp]
    #25352004 - 07/28/18 10:17 AM (5 years, 6 months ago)

Grain water is  very clear like light malt extract.


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