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pixelpopper
Crap Artist

Registered: 09/20/13
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Re: Pasty Agar Tek [Re: plurfekt]
#25220746 - 05/22/18 12:06 PM (5 years, 8 months ago) |
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Yeah, this isn't my first go with agar or pasty plates.. have made them before successfully - I was just wondering why everyone is so anal about flat agar
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tryptkaloids
Learner



Registered: 02/08/15
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The main thing is making sure there's no media on the rim that can wick contams into the plate
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,163
Loc: rural ghetto
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if you put in 5 layers of tape, instead of 1 or 2, will you fuck these plates up? like maybe slow down the evaporation of condensation
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
Edited by pablokabute (05/22/18 12:31 PM)
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tryptkaloids
Learner



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Naw
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
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The only reason for tape is preventing an imploding vacuum during the pc cycle.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
pixelpopper said: Yeah, this isn't my first go with agar or pasty plates.. have made them before successfully - I was just wondering why everyone is so anal about flat agar
It just looks better. I find viewing the grown out specimens is very mildly improved as well.
How I've been doing mine:
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pixelpopper
Crap Artist

Registered: 09/20/13
Posts: 4,022
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Re: Pasty Agar Tek [Re: plurfekt]
#25220820 - 05/22/18 12:43 PM (5 years, 8 months ago) |
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That all makes sense and is what I figured
Just wanted to make sure there wasn't something I was missing since it seemed very important in many posts
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plurfekt
Finally Grateful


Registered: 12/14/09
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Loc: USA
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Quote:
pixelpopper said: That all makes sense and is what I figured
Just wanted to make sure there wasn't something I was missing since it seemed very important in many posts
You're gucci mayne.
I've yet to see contamination on any agar. I'd love to know how to fuck these up, ya kno? :P
Two months ago I'd never bothered with agar and clicked on the link in JHOVA's signature. (Thank you JHOVA/Pasty - I know I'm not the easiest person to like, but I appreciate it.)
Now I have my first isolates and this next week, clones. Also have 3 tubs from agar>LC>wbs>cvg that dominated the CVG, clearly very healthy mycelium/aggressive. When I spawn tubs I do it with a new-found confidence that I won't see the mean green or cobweb. Sterile technique with agar and proper pasteurization of substrates are paramount in knocking out vectors for failure.
Done maybe 60 plates so far, not a hiccup besides learning the easy procedure/s in the SAB.
I invested in agar the "convenient, more expensive way" and still only spent like 75$ for everything. I'd regret it if I didn't plan on advancing further - one could go their entire life without touching a real petri dish or pre-mixed agar and still produce the same results as any professional when using the right techniques.
This TEK resulted in sending me to pour agar within two months, and now I'm sitting here like "Why even bother with pour teks except for proven isolates and clones?"
The awesomeness just keeps on coming. I don't even think I need a flowhood anymore.
Edited by plurfekt (05/22/18 12:56 PM)
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tryptkaloids
Learner



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Re: Pasty Agar Tek [Re: plurfekt]
#25220841 - 05/22/18 12:54 PM (5 years, 8 months ago) |
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I doubt you have isolates yet. Throw one on a 100mm plate at 1.5g/100ml and wait for sectors. Quote:
plurfekt said:
Quote:
pixelpopper said: Yeah, this isn't my first go with agar or pasty plates.. have made them before successfully - I was just wondering why everyone is so anal about flat agar
Lumps and depth can affect apearance of the myc. It reacts differently to different access to resources
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
Edited by tryptkaloids (05/22/18 12:55 PM)
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pixelpopper
Crap Artist

Registered: 09/20/13
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Quote:
tryptkaloids said: Lumps and depth can affect apearance of the myc. It reacts differently to different access to resources
This also makes sense and I do generally try to adhere to even mixing in all regards. Flat wasn't quite the correct term for what I meant, because I still have a flat surface, it just isn't parallel with the bottom of the dish. I suppose it has varying depth from sides to middle, but still its not like lumps
Edited by pixelpopper (05/22/18 01:02 PM)
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plurfekt
Finally Grateful


Registered: 12/14/09
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Loc: USA
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Quote:
tryptkaloids said: I doubt you have isolates yet. Throw one on a 100mm plate at 1.5g/100ml and wait for sectors.
I doubt I care what you think of my grow which you are not running. The people I learned the techniques from have had the same success in the same timeframe by using smaller biopsies for each transfer.
I used a needle to do biopsy transfers, you couldn't even see the flesh. I just saw my first two isolates four transfers in.
(Not like I'm stopping here anyway...)
Edited by plurfekt (05/22/18 01:08 PM)
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tryptkaloids
Learner



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Re: Pasty Agar Tek [Re: plurfekt]
#25220925 - 05/22/18 01:31 PM (5 years, 8 months ago) |
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I'm just saying I've followed isolation threads and they take forever and tend to only sector well on a larger surface than mini rounds provide
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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plurfekt
Finally Grateful


Registered: 12/14/09
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Loc: USA
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Quote:
tryptkaloids said: I'm just saying I've followed isolation threads and they take forever and tend to only sector well on a larger surface than mini rounds provide
I know, it's basic agar to know many transfers are usually needed.
Hence the biopsy tek to speed things up. I'll prove it is an isolate 100% before using it on any experiment or w.e - pretty sure I'm good though.
I have 100mm plates and MEA agar, I'll figure it out.
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Re: Pasty Agar Tek [Re: plurfekt]
#25238915 - 05/30/18 06:25 PM (5 years, 7 months ago) |
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Here we go
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TEKs I Like
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
MadHatter333 said: Here we go

-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Red mill is such a popular brand. See it every where on the webs on mush pictures. Only BRF brand I've found in the 100 mile radius has been red mill. There rye berries are bit too spendy so I've been using white millet. Makes one wonder how much of there intake is from urban mushroom cultivators. 
Loving the tek btw so simple and easy except for the viewing side of things. Found shining a flash like at various angles kind of helps.
Got some nice clear deli containers that are rated pp5. Seem a bit thin so I'll have to do a test run with the samples I got before investing in a whole box of em.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Bobs Red mill is definetly a good brand. I use their brf and now potato flakes. This will be a first for me using these plates with prints I got from my first trades. I also am pc’ing a bunch of long qtips to use for inoculation.
Got the pc running meow
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
MadHatter333 said: Here we go

welcome to the clutch

i just did another 10 plates alongside some lc's on Tues, six transfers (5th transfers, isolates or not...)
but something fun: old syringes from back in the day i'm pretty sure are dirty; put some PE6 and Thai Lipa Yai on plates via loop - looking to isolate the healthy tissue and keep the varieties in my collection. Only sure the PE6 was dirty.
Fair to say it's pretty damn pointless to keep anything but a few spore prints every 5 years or so for each variety, do the rest on agar.
Trusting your opinions and trying out green food coloring over blue for improved visibility.
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MadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
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Re: Pasty Agar Tek [Re: plurfekt]
#25240128 - 05/31/18 09:40 AM (5 years, 7 months ago) |
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Thanks bud
What should I use to scrape the spores on to the qtips I’ll be using? This will be my first time and I want to do it right. I have some metal racks I’ll be using to keep the plates off the surface. I hear opening/inoculating the plates upside down is the way to go.
I feel like a noob again, but I should have started this a long time ago.
Sounds like you got some good work ahead of you plurfekt
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thelanzii

Registered: 11/13/12
Posts: 5,434
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munch has a video of him inoculating and making transfers with no pour
he also has one using a swab under the tiger drop method
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