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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#25194488 - 05/09/18 10:17 PM (5 years, 8 months ago) |
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plurfekt
Finally Grateful


Registered: 12/14/09
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#25194798 - 05/10/18 05:19 AM (5 years, 8 months ago) |
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Quote:
Pastywhyte said: I never store blank plates in the fridge. You want any contamination present to grow so you can see it. We only use the fridge to slow down cultures we want to keep.
I only intend to keep "winning" isolates/clones in the fridge, so to speak. I only have 9 plates at the moment, and a bunch of empty prepped mini rounds. Agar is cheap even when you buy premixed premium shit.
I've yet to see a plate contaminate at all. Pretty positive the first tub from agar spawn is a winner, too - given the aggressive mycelium and zero signs of contamination. I got lazy with one of my SAB sessions (long day at work, lighter ran out of fluid) and went about the rest of the tranfers in haste, even smacking the scalpel against the lid, etc - and I still didn't see any contamination.
Current tub is just MS>Agar>LC, but will be good for taking clones which I also intend to put the "winners" on ice. I'm now into third transfers (fourth set of plates) - these very well may be isolated, I'll know in a few days or so.
Anyone have any tips on visibility? So far I just hold mine to a 6500K flouro and try my best to see what I can.
EDIT_
@ Pasty ~ This does make sense though, I hadn't thought of it that way - allowing the contaminants the opportunity to grow to see if they are there. The container I keep them in is basically a shoebox sized SAB without arm holes, I keep the lid cracked and I clean it out with 70% iso like once every 2-4 weeks. Ordinarily I just knock out any vector for possible contamination but in this instance it does make sense; especially for blended agar LI and such.
Edited by plurfekt (05/10/18 05:27 AM)
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
#25195513 - 05/10/18 12:47 PM (5 years, 8 months ago) |
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theres no need to replace the paper towel on the lid every pc cycle correct?
mine are on there pretty good
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VoiceOfLogos


Registered: 01/31/17
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25195638 - 05/10/18 01:38 PM (5 years, 8 months ago) |
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ugh so im about to put my pastys in the PC and i just realized i forgot paper towels. and i dont have any. has anyone had sucess with out the paper towel? i use a double layer of micro pore tape anyways so i dont really see the paper towel keeping out any contams. i dn we shall see i guess...
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thelanzii

Registered: 11/13/12
Posts: 5,434
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the paper towel is for lessening condensation not for filtration
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VoiceOfLogos


Registered: 01/31/17
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hm, ya i looked back at pasty's original post and he did mention the side holes and how they could remove the need for towels and foil so i should be good. didn't make side holes but i doubled up on the foil and i use jar rings and a plate to lift them up. and also use crumpled foil to keep everything in place. condensation isn't really an issue. i never get much but if it causes any water to pool i just open my pasty upside down when the time comes and it will just pour out. but ive always given mine time to cool on the burner. which most people say makes a difference. honestly though the mycelium will end up sucking that shit up anyways. they dry pretty quick once inoculated and before that its all sterile so it shouldn't cause any issues with mold or anything.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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my only advice would be, if you remove the foil and find that the tops and tape are wet, let the chill in the sab or flow until the tape dries.
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VoiceOfLogos


Registered: 01/31/17
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Quote:
verum subsequentis said: my only advice would be, if you remove the foil and find that the tops and tape are wet, let the chill in the sab or flow until the tape dries.
ok ya thanks. i usually do leave mine wrapped in the foill but i probably will unwrap these soon after they come out of the pc just to check them over.
ill probably just unwrap them in my SAB and i have little shoebox type tubs that ill just put them all in and ill just do all that in the sab so nothing lands on the tops or in the little box and then from there i could move em around. its funny i used to be so crazy about my plates but they really wont contam unless you open them.
also its pretty crazy im on my like 3rd transfer of these PEs and im pretty sure i have a mono culture. ill try and get some pics up later. u guys ever get one that fast from spore?
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verum subsequentis
seeker of truth



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sounds good. i seriously doubt you have an ISOLATE after three transfers. how did you apply the spores to agar? did you dilute the shit out of your spores?
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VoiceOfLogos


Registered: 01/31/17
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ya i dn honestly. before this ive had the same corumba brazils forver that i eventually took a clone from and used that forever. and i just got these PE spres from a freindly hawk. nice cluster of spores in the syringe and i put a few drops. transfered saw a few very defined sectors and started taking samples. so far 1 plate i have is just a perfect circle. the other one has a few sectors still. i dn it looks like one to me. but i dn cuz before this i always just used the clone. pretty crazy. im gonna do a transfer and use the leftovers for a jar or 2.
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VoiceOfLogos


Registered: 01/31/17
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but ya whether i post the pic publicly or not ill make sure i at least message it to u or something. cuz im curious.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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i won't be able to say for sure by looking at it but i'd like to see it. all i know is that i've had many cultures that i thought were isolated that turned out not to be. i used to mark the with an m if i thought they were there but after watching dozens of cultures appear isolated for three or four transfers and then explode into sectors on the next has changed my mind. it's pretty crazy what can be packed into that little dish,
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bender466
Stranger


Registered: 04/15/18
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sab stands for? not sure. why tmight just do i loop method his. tek. confuses me might try. it.
Edited by bender466 (05/10/18 03:51 PM)
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Pastywhyte's Easy Agar Tek [Re: bender466]
#25195937 - 05/10/18 03:58 PM (5 years, 8 months ago) |
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still air box where inoculation takes place
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25197891 - 05/11/18 03:56 PM (5 years, 8 months ago) |
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Made my first pasty plates today.  I found using a small funnel with a 1/4-1/2 inch hole works good if you don't want to spoon the molten agar into the bowls. It makes the liquid flow uniformly from the middle until you get your desired depth.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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plurfekt
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Registered: 12/14/09
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Re: Pastywhyte's Easy Agar Tek [Re: R.I.P.Zappa]
#25199297 - 05/12/18 11:08 AM (5 years, 8 months ago) |
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Quote:
R.I.P.Zappa said: Made my first pasty plates today.  I found using a small funnel with a 1/4-1/2 inch hole works good if you don't want to spoon the molten agar into the bowls. It makes the liquid flow uniformly from the middle until you get your desired depth.
How does this save time though?; I just pour mine in with a mason jar and a winter glove and have zero problems so it seems like an unnecessary step for a no-pour tek.
I find once pc'ed the agar liquifies and all that can give a uniform/level finish is allowing them to cool/dry in a SAB on a flat surface; this makes them as perfect to flat as possible, and your media should have been properly mixed/melted prior to pouring.
I re-use the paper towels, they fit easier the 2nd/3rd/4th/etc time you put them on the mini round lids, lol.
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tryptkaloids
Learner



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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
#25199319 - 05/12/18 11:24 AM (5 years, 8 months ago) |
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Fuck a spoon, fuck a funnel. Pour straight from the pot
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Quote:
tryptkaloids said: Fuck a spoon, fuck a funnel. Pour straight from the pot 
This is what I do
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
#25199554 - 05/12/18 02:11 PM (5 years, 8 months ago) |
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Just the mention of spooning it in implied to not getting it every ware. Besides this is right out of the pot so no media bottle or mason jar would even be necessary since they are being PCed after pour anyways. just trying to keep it simple stupid and efficient. Just figured I'd throw that out there for those who are spooning or worried about pouring molten agar everywhere or using extra steps.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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Dactylium
Don't touch me I'm sterile

Registered: 08/12/16
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Quote:
tryptkaloids said: Fuck a spoon, fuck a funnel. Pour straight from the pot 
I pour from a media bottle
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