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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
    #25168397 - 04/27/18 05:15 AM (5 years, 8 months ago)

Quote:

EtherealMind said:
put holes in 24 lids today

soldering iron worked like a champ

excited to get these plates rolling and in some liquid inoculant




I should try a soldering iron; just been taking a dremel to the lids after the bit to clean them up a bit.

Was going to do it yesterday but had a long work day (pay day and half day today bitches!) ; I have so many transfers to do I actually don't know how many transfers I should actually do.

Like, per original plate, I made 4 transfers from Syzygy, 6 from PE.  All of them appear healthy, although I did note some extreme isolate action going on in one of the syzygy plates - so I'll start with those sectors.

But still, if I were to even do 4 per plate, that'd be 40 plates just off of 10.

How do you guys go about selection or do you just keep buying more mini-rounds?  Cuz so far that seems like the logical next move :lol:


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25168655 - 04/27/18 08:28 AM (5 years, 8 months ago)

Have you cloned yet? That seems like a quicker rout to the long process of isolating. What do you want an isolate?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25169689 - 04/27/18 04:31 PM (5 years, 8 months ago)

Quote:

tryptkaloids said:
Have you cloned yet? That seems like a quicker rout to the long process of isolating. What do you want an isolate?




Tubs at 70-80% WBS>CVG ; my final MS>LC spawned tubs.

I have IDFK pasty plates, LC's and WBS in quart jars and bags.

Doing transfers now.  Just picked the plates with the best sector view.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25169862 - 04/27/18 05:42 PM (5 years, 8 months ago)

None of that answers my question... Why are you aiming for an isolate?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineBaby_Hitler
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25170688 - 04/28/18 12:00 AM (5 years, 8 months ago)

Why would anyone aim for AN isolate(ed strain)?

I can see why they would aim for a shit ton of isolated strains which they could grow out and collect data on to select the best performers. Same for clones except merely several instead of a shit ton.


--------------------
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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25170900 - 04/28/18 04:01 AM (5 years, 8 months ago)

Quote:

tryptkaloids said:
None of that answers my question... Why are you aiming for an isolate?




The same reason people take clones; for better/different results to test out individually - I'm cloning as well though.

I thought telling you my tubs were not fruiting yet was one way of saying "yo, I have no living fruits to clone yet" ; which sucks because by the time I was into agar I'd gone through just a fuck-ton of prize fruits I wanted to clone.

Did 13 transfers (2nd transfers) last night.  I'll happily be cloning some fruits in the next couple of weeks; that one mono is damn close to fruiting.

Condensation goes away with colonization; by the time I take my pastyplates to the SAB for transfers there isn't any.  Then it's just the plastic.  The plastic is the bane of my existence while trying to locate rhizo sectors.  Looking into other PP5 containers inspired by this TEK, but sticking with the OP for now.

Oh and...it's addicting, all these plates, lol.  Verum puts my attempts at PE isolates to shame, IDK how he keeps track of all of that, impressive logging.


Edited by plurfekt (04/28/18 04:10 AM)


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OfflineDoc9151M
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25170926 - 04/28/18 04:28 AM (5 years, 8 months ago)

An Isolate should give consistency to the grow. You would use an isolate for experiments as well. An isolate should have same sized fruits, even fruiting and consistency to the potency.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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Invisibleplurfekt
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Registered: 12/14/09
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Re: Pastywhyte's Easy Agar Tek [Re: Doc9151]
    #25170930 - 04/28/18 04:32 AM (5 years, 8 months ago)

Quote:

Doc9151 said:
An Isolate should give consistency to the grow. You would use an isolate for experiments as well. An isolate should have same sized fruits, even fruiting and consistency to the potency.




That's the idea, anyways.

Takes a few transfers to get one though, I've heard it takes some people quite a few.  For this reason I've decided to keep my plate count down and focus on the best of each batch visibility wise.


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OfflineDoc9151M
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt] * 1
    #25170936 - 04/28/18 04:37 AM (5 years, 8 months ago)

That's why I use a needle biopsy method, it's easier to stay inside of a sector.


That's after 1 transfer from a tomentose plate, now it's rhizomorphic growth will allow me the see the sectors easier.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Edited by Doc9151 (04/28/18 04:39 AM)


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Invisibleplurfekt
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Registered: 12/14/09
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Re: Pastywhyte's Easy Agar Tek [Re: Doc9151]
    #25170949 - 04/28/18 05:00 AM (5 years, 8 months ago)

Quote:

Doc9151 said:
That's why I use a needle biopsy method, it's easier to stay inside of a sector.

That's after 1 transfer from a tomentose plate, now it's rhizomorphic growth will allow me the see the sectors easier.




I agree a needle would be easier; I just did a set of LC's using the biopsy/poke method and they turned out great.  I actually had one in my SAB for the process but I was just being stubborn and trying to get the hang of the scalpel lol, may just opt for a 1.5" needle next time.

The ones I did via inoc loop last time did just as well as the scalpel, I imagine no difference with a needle, except less cells/tissue taken which in this case is a good thing.  I think I'll give it a go.

Funny blooper happened last night:  My propane torch ran out of propane and my BIC died at the same time.  I'd had that propane torch for 2+ years, and that bic for 3+ months.  I'm sitting there doing transfers like "seriously, are you fucking kidding me?" after a long week at work and a bunch of personal family drama; I'll admit those last few transfers I was a little...impatient...deliberate...lol - like the agar wouldn't come off the damn scalpel so I just smacked it against the lid so it would fall off.  I was like FUCK IT; I WANT TO GO TO BED.

Ended up using a few extra scalpel blades than planned, good thing they are cheap! :lol:


Edited by plurfekt (04/28/18 05:16 AM)


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OfflineDoc9151M
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25171161 - 04/28/18 08:01 AM (5 years, 8 months ago)

I've used the same blade for a year now. A needle is easier for me to use than a scalpel at times, I get better results when I use the needle for transfers and spores. I like the needle for spores because it will trap tjem inside the agar and they germinate really good, especially paneaolous and other difficult to germinate species. Some people have to pour warm agar on their pan spores for them to germinate but with the needle it's not necessary.


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: Doc9151]
    #25171511 - 04/28/18 11:24 AM (5 years, 8 months ago)

Quote:

Doc9151 said:
I've used the same blade for a year now. A needle is easier for me to use than a scalpel at times, I get better results when I use the needle for transfers and spores. I like the needle for spores because it will trap tjem inside the agar and they germinate really good, especially paneaolous and other difficult to germinate species. Some people have to pour warm agar on their pan spores for them to germinate but with the needle it's not necessary.




Cool info on the pan spores.  I've had several other actives in previous collections I should really re-obtain to grow out now that I am able.

I had to re-use blades due to no flame to sterilize with, that and they get all crapped up from the BIC lighters.  Just did it with what I had on hand, not worried about a few dollars.

You have a link for agar to agar w/ poke tek?  I'm wondering about the amount of water (if any) used to squirt.  Seems you wouldn't even need to do that.


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Invisiblethelanzii

Registered: 11/13/12
Posts: 5,434
Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25180424 - 05/02/18 05:40 PM (5 years, 8 months ago)

do you guys retape the lids when used again?

i double taped all of mine


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii] * 2
    #25180475 - 05/02/18 06:08 PM (5 years, 8 months ago)

i havent changed the tape on my lids in years


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Invisiblepablokabute
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #25186564 - 05/05/18 08:05 PM (5 years, 8 months ago)

I have experienced storing these plates upside down causes problems, condensation and a bit of nutes-rich liquid go to the lid, then get all sorts of mold, opening it up causes the molds spores to blow over the surface and boom..


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: pablokabute]
    #25187215 - 05/06/18 08:03 AM (5 years, 8 months ago)

Quote:

pablokabute said:
I have experienced storing these plates upside down causes problems, condensation and a bit of nutes-rich liquid go to the lid, then get all sorts of mold, opening it up causes the molds spores to blow over the surface and boom..




I never store plates upside down


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Invisiblepablokabute
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #25187527 - 05/06/18 10:55 AM (5 years, 8 months ago)

Quote:

Pastywhyte said:
Quote:

pablokabute said:
I have experienced storing these plates upside down causes problems, condensation and a bit of nutes-rich liquid go to the lid, then get all sorts of mold, opening it up causes the molds spores to blow over the surface and boom..




I never store plates upside down





some of your readers do and I think its a bad practice. just putting it out here for others... :smile:


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: pablokabute]
    #25187548 - 05/06/18 11:02 AM (5 years, 8 months ago)

:whathesaid:


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Offlineverum subsequentis
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #25187592 - 05/06/18 11:23 AM (5 years, 8 months ago)

Never tried it because there is no need too. I also remember the old rabbit talking shit on it.


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InvisibleMushMan17
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #25187615 - 05/06/18 11:36 AM (5 years, 8 months ago)

Thanks Pasty. I'm having no issues using this tek with a poly-fill hole on the side of the container and no paper towels or foil.


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