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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
#25160225 - 04/23/18 03:32 PM (5 years, 9 months ago) |
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Pe blobs can be prevented by: adding a casing layer, consolidating jars, delaying fruiting conditions after reaching 100%.
Azur and lotkid have already demonstrated how to avoid blobs.
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kanemush
Grumpy

Registered: 12/07/17
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Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
#25160242 - 04/23/18 03:44 PM (5 years, 9 months ago) |
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Depends what your doing if there going to grain I like at least a quarter sized sample. If I am trying to isolate or clone then I pull the sample when its no more then a dime sized spot.
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Pastywhyte's Easy Agar Tek [Re: kanemush]
#25160399 - 04/23/18 05:07 PM (5 years, 9 months ago) |
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any tips on getting spores to germinate asap
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25160427 - 04/23/18 05:20 PM (5 years, 9 months ago) |
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Quote:
EtherealMind said: any tips on getting spores to germinate asap
Use fresh spores. Softer agar helps too.
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JiM aYYYY
GreenGoblin


Registered: 03/06/18
Posts: 266
Last seen: 4 years, 1 month
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25160431 - 04/23/18 05:21 PM (5 years, 9 months ago) |
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Have spores that were from your best fruit in agar(find a happy medium in nutes). Store to start colonization at 80 degrees any hotter and it will start to slow down again. Keep it in a clean area to avoid contamination(high heat promotes bacteria)but this is from reading and my current experience with Ms and agar. Tried a little experiment last week with it and it still took 3 days. 3 days is Pretty fast as I've seen with anyone so far. If you find anything out that's good please share it
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Sparky101
Electrical Guru


Registered: 02/14/18
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I pc’d some plates last night and just realized I forgot to add the honey. I used the pda recipe. Is this a problem? Will they still be usable?
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JHOVA
Post whore


Registered: 02/17/17
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The recipe works with or without honey/ karo syrup.
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Re: Pasty Agar Tek [Re: JHOVA]
#25161348 - 04/23/18 11:45 PM (5 years, 9 months ago) |
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Funny, only time I ever got blobs was with a casing layer using MS.
Exception to every rule I guess. I've only had two tubs out of dozens blob on me, and those were the ones with casing layers; the substrate I was using at the time didn't require them or so they say (and I have no reason to doubt them). I always consolidate and back then I always delayed fruiting conditions with contractor bags, so who knows what the deal is - in pure multi-spore mine literally did not blob over 9 times out of 10.
All I know is PE doesn't need a casing layer via first hand experience. Maybe it helps, in my experience all that helps with is pinning/avoiding aborts.
(oh and cleaning vermiculite off blobs fucking blows, i just used a scalpel )
Since switching to CVG, I haven't seen a blob with or without a casing layer. Luck?
I am about to make my first blended LI, with a sexy lookin' PE pasty puck (2nd transfer). SAB been sitting an hour, g**dam*** I have to be awake for work in like 3.5 hours so I best get to this; have to cut the agar in sections due to my blender now allowing for wide-mouthed mason jar entry, I cannot tiger drop without hitting the lid brim (I think, if I can get it to bend in the mini-round, maybe. EDIT: it worked fine, lol - 1 second tiger drop to dh2O)
I'm already up to like 30 pasty plates but I think I'm gonna go buy several more boxes; the transfers can get out of hand pretty quickly, may even toss some of these that don't look as perfect as the others, lmao.
I've gotta say, cloning at first makes a lot more sense...just no way to know what an isolate will do until it does it...but I guess MS was the same way just worse
This puck looks so pretty in its blender pint jar. It's almost a shame to blend it, this is the best visibility I've had of a culture yet (bottom of jar holding jar against light)
Edited by plurfekt (04/24/18 12:52 AM)
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#25161807 - 04/24/18 08:21 AM (5 years, 9 months ago) |
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Quote:
Pastywhyte said:
Quote:
EtherealMind said: any tips on getting spores to germinate asap
Use fresh spores. Softer agar helps too.
no idea how fresh these spores are
i used brf agar
the formal definition of soft agar is less nutrients?
can you point me in the direction of some soft agar recipes
Edited by thelanzii (04/24/18 09:35 AM)
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JiM aYYYY
GreenGoblin


Registered: 03/06/18
Posts: 266
Last seen: 4 years, 1 month
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25161885 - 04/24/18 09:26 AM (5 years, 9 months ago) |
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VoiceOfLogos


Registered: 01/31/17
Posts: 71
Last seen: 3 years, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25162532 - 04/24/18 02:24 PM (5 years, 9 months ago) |
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the pda recipie I use (pastys) always seems a little soft to me so I usually use like 2.68 grams of agar instead of just 2.5 cuz i prefer it a little stiffer. it just cuts better for me. and is easier to work with.
so maybe try that recipie or just add a little more water than usual. shouldn't take much to make a difference.
but honestly i would just make a lot of plates. for example i just inoculated 2 PE plates and 1 pancyan plate and the 1 pe is the only one growing so far. some plates will start earlier than others from spore so to give yourself better odds id say noc up a few plates. also spores to agar shouldn't take more than a few days to see growth. definitely under a week.
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VoiceOfLogos


Registered: 01/31/17
Posts: 71
Last seen: 3 years, 3 months
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and thanks for all the advice on the PEs guys. ill probably honestly try a few different things. i should have atleast 3 tubs fruting for the first grow of them so ill try some different shit. been doing corumba brazils for the past year so I'm exited to try something new! also a little nervous. the corumbas did right by me definitely and i have a pretty decent little clone. still going to run them. learned a lot from growing those things and now i really feel like a true mycologist. cant wait to see how far ive come in another year.
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thelanzii

Registered: 11/13/12
Posts: 5,434
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i have 12 plates inoculated
i saw growth on one today it has been 7 days which isn't abnormal from the time frames i have seen
saw growth on the glass of one today
looks like mycellium but im not fully sure
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thelanzii

Registered: 11/13/12
Posts: 5,434
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how long does grain water agar take to pressure cook?
12g brf per liter 18g-20g agar per liter with spring water
could it be used the same as this recipe just substituted wit grain water?
i inoculated grain jars with spore syringe and they are showing more growth in the same amount of time as my brf agar
why is that?
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii]
#25164255 - 04/25/18 10:29 AM (5 years, 9 months ago) |
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Just put agar in grain water maybe half grain water half tap
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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boom boom

Registered: 02/27/18
Posts: 429
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It'd probably be best to try the half and half water/grain water first, with no brf. Then increase from there if needed. I've seen a lot of people recommend half and half so it obviously works for a lot of them. The less nutrients that are in the agar, the more rapidly the mycelium will grow outwards searching for them, so shoving in all the nutrients you can into your agar is actually counterproductive.
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Pastywhyte's Easy Agar Tek [Re: boom boom]
#25164285 - 04/25/18 10:45 AM (5 years, 9 months ago) |
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That's not entirely true. No nutes won't work. Too many will give you a lazy culture. Aim for the sweet spot.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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boom boom

Registered: 02/27/18
Posts: 429
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I didn't Quote:
tryptkaloids said: That's not entirely true. No nutes won't work.
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Pastywhyte's Easy Agar Tek [Re: boom boom]
#25167735 - 04/26/18 07:48 PM (5 years, 8 months ago) |
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put holes in 24 lids today
soldering iron worked like a champ
excited to get these plates rolling and in some liquid inoculant
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Pastywhyte's Easy Agar Tek [Re: thelanzii] 1
#25167798 - 04/26/18 08:21 PM (5 years, 8 months ago) |
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you're on the right track
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