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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25103018 - 03/30/18 07:08 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Now I'm confused...




It's premium sponsor MEA, I was originally gonna pour petris and my damn whiskey bottle wouldn't fit in PC, so mason jarred the bitch in the fridge and used the MEA for the pasty plates.


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Invisiblepablokabute
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25103135 - 03/30/18 07:57 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Quote:

pablokabute said:
hi guys, easiest modification of these pastyplates for long-term storage? :smile: thanks



Pour them thick. Refrigerate.

Sfds will work but,it's a waste imo





and I was like.. thinkin.. add popsicle sticks in there also?


--------------------

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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25103642 - 03/31/18 02:31 AM (5 years, 9 months ago)

Quote:

plurfekt said:
Quote:

tryptkaloids said:
Now I'm confused...




It's premium sponsor MEA, I was originally gonna pour petris and my damn whiskey bottle wouldn't fit in PC, so mason jarred the bitch in the fridge and used the MEA for the pasty plates.


How did you pour set agar? Did you give it a hot water bath?


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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25103710 - 03/31/18 04:00 AM (5 years, 9 months ago)

When I made it up for the initial pour it was in hot distilled water.  Ofc, in the fridge there was some settlement of sediment but I found a good shake between each mini round did just fine.  It wasn't in the fridge very long.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25103963 - 03/31/18 08:38 AM (5 years, 9 months ago)

Was it solid before you put the lids on? I wait for it to stop steaming all together


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25104681 - 03/31/18 01:56 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Was it solid before you put the lids on? I wait for it to stop steaming all together




It was liquid, solidifies when it reaches a certain temperature.  It's premium/sponsor premixed MEA.

Quite possible the telephone agar is better for this method condensation wise, I had considered that, but it still worked.  At this point it's on me for sterile technique.

JHOVA's recommendation to remove the liquid with a light flick is very logical in my situation.


Edited by plurfekt (03/31/18 01:59 PM)


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25104785 - 03/31/18 02:48 PM (5 years, 9 months ago)

Let it solidify first is all I'm saying.  I have never used telephone agar, it should work regardless of brand


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25104910 - 03/31/18 03:42 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
Let it solidify first is all I'm saying.  I have never used telephone agar, it should work regardless of brand




I wonder how hot this needs to be to solidify, I just ran the bottle under hot water for 15 mins and shook it well, but once it was in the fridge it was below room temperature and still wasn't solidifying - I used a digital scale at the proper ratio.  They look fine now that they are PC'ed, as they should, minus the condensation.

Any technique for doing this w/ MEA hygroscopic powder?


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25105146 - 03/31/18 05:41 PM (5 years, 9 months ago)

I put water, agar and mea in a pot, turn to high, stir until boiling. Then I pour into my pasty plates. Wait 15 minutes or so then lid them and pc... Idk what temp is solidifies at


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #25105373 - 03/31/18 07:44 PM (5 years, 9 months ago)

Quote:

tryptkaloids said:
I put water, agar and mea in a pot, turn to high, stir until boiling. Then I pour into my pasty plates. Wait 15 minutes or so then lid them and pc... Idk what temp is solidifies at




I'll give this a go on my next set, I appreciate the input; I wouldn't have known otherwise. :mushroom2:

edit:  I went and bought 8 more mini rounds, so I'll be giving this a shot sometime this week - starting a new job or I'd do it tomorrow.


Edited by plurfekt (04/01/18 01:46 PM)


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Offlinebandit864
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25107536 - 04/01/18 10:46 PM (5 years, 9 months ago)

I always have condensation in mine, but it normally goes away with 3-5 day.


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Invisibleelasticaltiger
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Re: Pastywhyte's Easy Agar Tek [Re: bandit864]
    #25107565 - 04/01/18 11:11 PM (5 years, 9 months ago)

Yeah people get too hung up on the condesation :shrug: it seriously doesn't affect anything unless you have an actual puddle on your agar.


--------------------
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Offlinebandit864
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Re: Pastywhyte's Easy Agar Tek [Re: elasticaltiger]
    #25107569 - 04/01/18 11:13 PM (5 years, 9 months ago)

Yeah it's never caused any problems so far. I love the simplicity of this tek. Thank you man. This made jumping into agar so much easier.


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Invisiblethelanzii

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Re: Pasty Agar Tek [Re: bandit864]
    #25147870 - 04/18/18 11:28 AM (5 years, 9 months ago)

how does brf agar do in pasty plates?

also is stacking these good practice?


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Offlineverum subsequentis
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Re: Pasty Agar Tek [Re: thelanzii]
    #25147900 - 04/18/18 11:41 AM (5 years, 9 months ago)

stacking is fine but can get messy quick if you try to stack too high.

i can't see any reason brf agar wouldn't work but have never done it.


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InvisibleJosex
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Re: Pasty Agar Tek [Re: thelanzii]
    #25147932 - 04/18/18 11:58 AM (5 years, 9 months ago)

Quote:

EtherealMind said:
how does brf agar do in pasty plates?

also is stacking these good practice?



Of course it works, it's a standard recipe, the container has nothing to do. You can stack mini rounds but the lids weren't made with that in mind, they have grooves so they don't stack well. I wouldn't stack more than 3 or 4, they can topple easily if you stack more than that.


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OfflineDoc9151M
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Re: Pastywhyte's Easy Agar Tek [Re: elasticaltiger]
    #25147948 - 04/18/18 12:04 PM (5 years, 9 months ago)

Quote:

elasticaltiger said:
Yeah people get too hung up on the condesation :shrug: it seriously doesn't affect anything unless you have an actual puddle on your agar.



After they cool and are solidified, store them upside down. You can even tap them lightly to knock the condensation down to pool on the lid, crack the lid slightly with the tab pointing down to release the water from inside the plate. Petri plates or pastey plates should never be stored where anything can settle on the agar surface. I even store my noc'd up plates upside down, I've only had one transfer fall off out of hundreds.


--------------------


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OfflineJiM aYYYY
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Re: Pastywhyte's Easy Agar Tek [Re: Doc9151]
    #25148030 - 04/18/18 12:39 PM (5 years, 9 months ago)

You could always modify the hole placement like he shows in his OP.


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Invisibleplurfekt
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Re: Pastywhyte's Easy Agar Tek [Re: JiM aYYYY]
    #25148390 - 04/18/18 03:08 PM (5 years, 9 months ago)

Did 10 transfers (4 syzygy, 6 penis envy) from my first 5 plates, did a botched blenderless inoculant (wrong lid, rofl) with the 6th puck I used to test my LC for cleaniness, it was clean, and the tubs from the spawn are colonizing nicely - first time agar confirmed something for me before I spawned a tub!

The transfers all look like they are recovering nicely after a few days, went with a little blue food coloring this time.  I did some by scalpel, some with an inoc loop.  I am going to use this other apparatus I don't know the name of next time.

I did pour these hot and allow them to solidify before PC'ing this time, and there was no change in condensation.  The good news is, the only reason it sucks is low visibility.

However, the condensation almost entirely disappeared on my first plates as they colonized more.

Loving agar and having great results so far, thanks much pasty!

:mushroom2:


Edited by plurfekt (04/18/18 03:10 PM)


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: plurfekt]
    #25149385 - 04/18/18 08:37 PM (5 years, 9 months ago)

Quote:

EtherealMind said:
how does brf agar do in pasty plates?

also is stacking these good practice?



I only stack up to 2 outside of the pc. You can fill your pc all the way if you have a later if glass no pours at the bottom. As long as they aren't floating and they go in straight you're good


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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