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mushboy
modboy



Registered: 04/24/05
Posts: 32,275
Loc: where?
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Re: Pastywhyte's Easy Agar Tek [Re: beerface]
#25045812 - 03/07/18 12:29 PM (5 years, 10 months ago) |
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buy agar powder and nute powder separately.. also when i used that exact same pda from ssulotions it said use 100g per 1750ml water horrible results with that. i got better growth by lowering the amount of powder but the agar was too soft since its premixed. so making transfers was impossible.
i had to let the poured dishes sit for about 2weeks before i used them. otherwise it was like taking a flamed blade to ice cream
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beerface
newguy



Registered: 01/16/18
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Re: Pastywhyte's Easy Agar Tek [Re: mushboy]
#25046280 - 03/07/18 03:08 PM (5 years, 10 months ago) |
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so at what point do I know if my agar containers arnt going to do anything? should there be any growth in one week? what is the longest to wait before tossing them and making a new batch and inoculating some new ones?
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Pastywhyte's Easy Agar Tek [Re: beerface]
#25046325 - 03/07/18 03:19 PM (5 years, 10 months ago) |
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After 30 days I use them for live myc. That is given contams aren't present. My suggestion is to make a second batch but softer
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Nunyabiz
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Registered: 02/08/18
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#25047264 - 03/07/18 07:59 PM (5 years, 10 months ago) |
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Greetings, after a successful pftek, I am gonna try monos. I did 30 mini round agars with the Thai agar packet, potato flakes, and Caro per the recipe . Questions..
Should the resulting concoction be translucent? Mine were opaque. I added green food coloring and they ended up a mint green. Not clear. I used cheap store brand potato flakes. Will using brf make them more likely to be clear?
3 days ago I sprinkled spores on top of the agar from a couple syringes for 25 of them. Should I have put the needle into the agar? I also used a sterile cotton applicator and a spore print I made for 5 of them. Most of the mini rounds have white spots with grey/black centers. Is that a sign of contam? Should they be pure white?
Lastly, when do I transfer to spawn? After the agar is entirely white? How long should that take? Thanks in advance for any help.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Re: Pastywhyte's Easy Agar Tek [Re: Nunyabiz]
#25047388 - 03/07/18 08:53 PM (5 years, 10 months ago) |
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Color doesn't matter, clearer recipes like mea only help for visability. You don't need to stick the needle into the agar. I think you are missing the point of using agar. We don't use it just to get into grain (we do, that's not the point) we use agar to clean up contaminates. I don't know what you are discribing, we need pics to give advice, but you should only take agar to anything after you ensure cube(i assume) mycelium is the only thing present
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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I like to use the needle to cut a little valley to drop my spores into.
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tryptkaloids
Learner



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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
#25047416 - 03/07/18 09:04 PM (5 years, 10 months ago) |
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I just meant it's not necessary, I was thinking more of like a pf style injection into the agar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Nunyabiz
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Registered: 02/08/18
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Last seen: 5 years, 6 months
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Thanks for the info.
The spores are GT, PB, and Camb.
I thought the point of Agar was to use it as a more concentrated medium to encourage mycelial growth with the starch and some sugar vs the vermiculite in the cakes. More simple, readily absorbed nutrients in the agar vs the verm. I didn't think of the decreasing contaminates factor. That makes sense. Thanks for the explanation.
I will try to put up some pics tomorrow. In the interim, how long, on average, does it take for a the agar to become completely penetrated with mycelium? (I am thinking of starting from scratch if I need to)
Thanks again for any info. This forum is an amazing resource!
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tryptkaloids
Learner



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Posts: 12,641
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Re: Pastywhyte's Easy Agar Tek [Re: Nunyabiz]
#25047611 - 03/07/18 11:06 PM (5 years, 10 months ago) |
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Quote:
Nunyabiz said: Thanks for the info.
The spores are GT, PB, and Camb.
I thought the point of Agar was to use it as a more concentrated medium to encourage mycelial growth with the starch and some sugar vs the vermiculite in the cakes. More simple, readily absorbed nutrients in the agar vs the verm. I didn't think of the decreasing contaminates factor. That makes sense. Thanks for the explanation.
I will try to put up some pics tomorrow. In the interim, how long, on average, does it take for a the agar to become completely penetrated with mycelium? (I am thinking of starting from scratch if I need to)
Thanks again for any info. This forum is an amazing resource!
The whole point is to have a 2d surface to know what you are growing. Hence why we need pics to tell you if you need to start over. Sounds like you have pin mold, are you using a sab? I need to know how much growth there is. https://www.shroomery.org/forums/showflat.php/Number/18430998 https://www.shroomery.org/forums/showflat.php/Number/22020260 Read through these
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Pastywhyte's Easy Agar Tek [Re: beerface]
#25047675 - 03/07/18 11:33 PM (5 years, 10 months ago) |
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Quote:
beerface said:
Quote:
Steevo said: The lids will do that. You just need to handle them very carefully.
Germination may take a while. What are your temps like where you're keeping them? Cooler temps will make things take longer in general
temps are low 70's
Quote:
mushboy said: i dont like that brand
is there a reason you dont like this brand? and does any one know a better premixed brand? I find it easier to get premixed as stated by rogerrabit in his videos too.
I haven't heard anything bad about the RAK media bottles, although I failed on my first attempt with it. They're a lot cheaper by gram than those packets on Amazon as well. 250g is like 35 bucks on any mushroom supply site.
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Pastywhyte's Easy Agar Tek [Re: boom boom]
#25048174 - 03/08/18 09:47 AM (5 years, 10 months ago) |
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Take 2 with rak MEA and unmodified ball jars. Tried using distilled water with the premix this time. I brought it all to a light boil on the stove while constantly stirring in a beaker to keep track of water loss. There was almost none. Consistency seemed a lot thicker when I was pouring this time. Filled up the PC with a little over 3 quarts to match the level of the agar and got it in the PC now. I'm gonna let it sit in there overnight, then I'll take them out and let them sit in my SAB until Sunday. Then I'm gonna try to germinate some spores. Hopefully I'll have better luck this time. The last plates I made, even the contaminants didn't seem to think were very edible.
Edited by boom boom (03/08/18 10:10 AM)
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Nunyabiz
Stranger

Registered: 02/08/18
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Last seen: 5 years, 6 months
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Here is a pic. Instead of placing the spores only in the middle, I dropped them from a syringe all over the agar. This is what nearly all of my agar mini rounds look like. Yes, I used a SAB. What do you think? Thanks!
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
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Re: Pastywhyte's Easy Agar Tek [Re: Nunyabiz] 1
#25050303 - 03/08/18 07:00 PM (5 years, 10 months ago) |
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Looks like straight mold. The dark discoloration emanating from the center is not good. Try again by flaming a xacto knife red hot. Cool xacto knife in your recipient jar agar. Squirt a drop of spore solution on the blade. Run the blade through the center for 1/2“. All in a sab. No alcohol or fire inside the sab.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 7 months
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Re: Pastywhyte's Easy Agar Tek [Re: JHOVA]
#25050617 - 03/08/18 09:49 PM (5 years, 10 months ago) |
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Do you have to run the blade through the agar? Is it still fine to just drop your spores from your syringe on top of the agar?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
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Re: Pastywhyte's Easy Agar Tek [Re: bandit864]
#25050670 - 03/08/18 10:13 PM (5 years, 10 months ago) |
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yeah. it's fine it just might roll around and make a mess that's harder to identify clean growth from. putting it on the blade and dragging it through the agar will streak the spores more or less. this will keep the water from rolling around and could possibly dilute you spore concentration a bit. i like to dig a little hole in the agar with my scalpel and drop the spore solution in the hole. if i'm not going to streak it.
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
Last seen: 5 years, 7 months
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Oh okay I'll give that a try next time. The 6 plates I did 2 days ago I just dropped a drop in each one. No signs of growth yet.
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bandit864
Edible learner
Registered: 09/15/17
Posts: 169
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Re: Pastywhyte's Easy Agar Tek [Re: bandit864]
#25054243 - 03/10/18 11:18 AM (5 years, 10 months ago) |
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How long do y'all normally start seeing growth from MS to agar? Mine are 4 days old with no growth. I also have some BRF substitute agar going a few days older and in one of the plates I see several small white fluffy growth. Hard to see inside though. I won't use BRF as a substitute ever again. So hard to see and during PC is bubbles up on the sides.
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tryptkaloids
Learner



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Re: Pastywhyte's Easy Agar Tek [Re: bandit864]
#25054442 - 03/10/18 12:35 PM (5 years, 10 months ago) |
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3 to 20 days
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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ButtFace
Registered: 08/06/08
Posts: 1,547
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Post deleted by TieACable2aTreeReason for deletion: double post
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boom boom

Registered: 02/27/18
Posts: 429
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Re: Pastywhyte's Easy Agar Tek [Re: bandit864]
#25057730 - 03/12/18 05:58 AM (5 years, 10 months ago) |
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Quote:
bandit864 said: Oh okay I'll give that a try next time. The 6 plates I did 2 days ago I just dropped a drop in each one. No signs of growth yet.
Did you ever end up seeing anything? Mine are 3 days in now and there's 0 signs of any growth. I'm trying to be patient but it's frustrating when for all I know I fucked up somewhere and ruined the plates or my spores are junk.
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