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Offlinedhype773
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Re: Pasty Agar Tek [Re: BreakTheSOB]
    #24578352 - 08/25/17 06:14 AM (6 years, 5 months ago)

Quote:

BreakTheSOB said:
I took a paperclip and straightened her out on one side, used loop side to hold



Nice!


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: ComebackKid]
    #24578369 - 08/25/17 06:37 AM (6 years, 5 months ago)

Quote:

ComebackKid said:
Quote:

mynakedrat said:
Well here goes.
Poured, lidded cooked.
9 psi lol. I'll double this 1hr39min






Hey man 20 mins at 9psi is more than enough to sterilize those plates next time :thumbup:




I don't agree. I have seen bacteria running short times with lower psi. Elevation and type of media also plays a role. Lab grade media usually has smaller populations and so can get away with less time.


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OfflineBreakTheSOB
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #24578452 - 08/25/17 07:52 AM (6 years, 5 months ago)

This is most certainly true, I live at around 2500 ft elevation, so water does not boil at 212 here. It's lower. So with that in mind I try to run at least 17 psi to get to normal pic temps for the guys running 15 at sea level where the atmospheric pressure is greater, something for all to keep in mind, be cognizant of your altitude and its effects on boiling points


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"A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle


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Invisiblemynakedrat
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Registered: 02/16/17
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Re: Pasty Agar Tek [Re: BreakTheSOB]
    #24578464 - 08/25/17 08:01 AM (6 years, 5 months ago)

I always do 2 hrs ish at 9.
My pc only Does 9 lol.
Plates look great, everytime


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OfflineSteevo
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Re: Pasty Agar Tek [Re: mynakedrat]
    #24578469 - 08/25/17 08:08 AM (6 years, 5 months ago)

40-45 @15psi just like the tek says. Tried, tested and true


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Invisiblemynakedrat
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Re: Pasty Agar Tek [Re: Steevo] * 1
    #24578471 - 08/25/17 08:10 AM (6 years, 5 months ago)

I wish I could change my psi :/
Which is why I triple it.
I like these new holes in the  lids though. Micro held up good.


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #24578674 - 08/25/17 10:21 AM (6 years, 5 months ago)

Hmm interesting. I must have just been getting lucky all this time then :justdontknow:
My electric pc that I use for agar is 11 psi and I only ever pc for 20 mins.
I assumed I should treat PDA like any other liquid media, no?


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: ComebackKid]
    #24578719 - 08/25/17 10:51 AM (6 years, 5 months ago)

higher populations, yet pasty is well above sea level. 20 minutes is fine at a low enough altitude


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24578749 - 08/25/17 11:03 AM (6 years, 5 months ago)

Yeah I should really be more aware of that damn altitude factor when giving advice


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Offlineblastedbasted
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Re: Pasty Agar Tek [Re: ComebackKid]
    #24588929 - 08/29/17 07:38 PM (6 years, 4 months ago)

im super newb to agar and trying my first attempt thanks to this tek. dont laugh at me here but do i use my spore syringe and just let a couple drops through the hole in the top after it has cooled overnight? if so, would i stab through the tape or peel it back to put the LC in?


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: blastedbasted]
    #24588960 - 08/29/17 07:45 PM (6 years, 4 months ago)

no dont stab through the hole. you have to remove the lid to inoculate it.


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OfflineDA Double G
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Re: Pasty Agar Tek [Re: blastedbasted]
    #24588963 - 08/29/17 07:45 PM (6 years, 4 months ago)

Just to be clear, are you trying to put a spore solution onto the the agar, or a liquid culture?
I think your talking about a syringe of spore water correct?


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Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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Offlineblastedbasted
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Re: Pasty Agar Tek [Re: DA Double G]
    #24588973 - 08/29/17 07:50 PM (6 years, 4 months ago)

yes, for ten years i have always used a syringe into a jar of PCed WBS. This is my first time doing anything different so im pretty clueless here.

Something is telling me a spore syringe (water and spores) is different than a liquid culture...?


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OfflineDA Double G
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Re: Pasty Agar Tek [Re: blastedbasted]
    #24588999 - 08/29/17 08:01 PM (6 years, 4 months ago)

Yes they are different things. I figured you were talking about a spore syringe though.:thumbup:
I haven't messed around with lc's yet. But Munchauzen answered your question.
Just be sure to put a small drop on each plate.
First time I tried I oversaturated it, "thinking it would be better" and fucked it up.
Good luck with everything and welcome!!


--------------------
Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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Offlineblastedbasted
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Re: Pasty Agar Tek [Re: DA Double G]
    #24589018 - 08/29/17 08:10 PM (6 years, 4 months ago)

Thanks much G! Ive literally been reading about agar for a month, finally found the powder in some oriental market downtown, and now that im ready to try i thought there was an entire step i was missing about getting the spore to the agar.

I suck at terminology here, i feel like a caveman when i read. Time to find a shroomery dictionary :laugh:


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: blastedbasted]
    #24589051 - 08/29/17 08:25 PM (6 years, 4 months ago)

https://www.shroomery.org/forums/showflat.php/Number/24297804 everything in this link will help you. especially this


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineDA Double G
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Re: Pasty Agar Tek [Re: blastedbasted]
    #24589074 - 08/29/17 08:31 PM (6 years, 4 months ago)

Your welcome,
Same thing here, oriental market downtown haha.
It took me a week to figure out what everyone meant when they said pasty plates.
I thought it was some kind of Petri dish that had sticky paste :facepalm3:
Would have been much easier if I just asked haha
Dont be shy when it comes to asking questions.


--------------------
Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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Offlinemekannic
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #24589272 - 08/29/17 09:53 PM (6 years, 4 months ago)

Quote:

FrankHorrigan said:
............you cannot pre-make petri dishes and then PC them, be them glass........... 







So petri dishes can't be used for a no pour tek??  I am slowly making my way through some of these type threads and I prefer the easy as fuck ways, not necessarily the EAF teks, but am always looking for easy, lazy, effortless ways.  I have disabilities that present definite challenges to what most find easy, if not tedious and laborious.

I was gonna try and use this style and use glass petris.  What is the problem?  boiling over?  Is there any chance of bringing the pressure up very slowly and preventing this?  Or is this even the (only?) issue?

Hope this thread ain't totally dead.  And yes I do know how to UTFSE, but it don't always find what you're looking for.


--------------------
A lesson on every Element of the Periodic Table
The Beautiful Math of Coral
Ice Loss Project
The Extreme Ice Survey Project



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"the leaf doesn't make it's own decision's.  Instead, it is the wind that decides where the leaf shall be, and how long the trip shall take"-m


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: mekannic]
    #24589284 - 08/29/17 09:55 PM (6 years, 4 months ago)

Quote:

mekannic said:
Quote:

FrankHorrigan said:
............you cannot pre-make petri dishes and then PC them, be them glass........... 







So petri dishes can't be used for a no pour tek??  I am slowly making my way through some of these type threads and I prefer the easy as fuck ways, not necessarily the EAF teks, but am always looking for easy, lazy, effortless ways.  I have disabilities that present definite challenges to what most find easy, if not tedious and laborious.

I was gonna try and use this style and use glass petris.  What is the problem?  boiling over?  Is there any chance of bringing the pressure up very slowly and preventing this?  Or is this even the (only?) issue?

Hope this thread ain't totally dead.  And yes I do know how to UTFSE, but it don't always find what you're looking for.



correct. boiling over. they are too shallow. bringing up the pressure slowly would make no difference. its still going to boil and its still going to spill out. don't do it.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24590393 - 08/30/17 09:41 AM (6 years, 4 months ago)

I have heard of some users finding tall ones that they got to work but it's not at all worth the hassle


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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