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Offlinenwcleaningllc
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Re: Pastywhyte's Easy Agar Tek [Re: Germs]
    #24539840 - 08/08/17 07:17 PM (6 years, 5 months ago)

How do you store the plates once they're done in the pressure cooker, and you're not going to use them for 2-3 days?


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: nwcleaningllc]
    #24539846 - 08/08/17 07:19 PM (6 years, 5 months ago)

On a shelf, in a drawer, in your SAB, don't overthink it.


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Offlinenwcleaningllc
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24539852 - 08/08/17 07:21 PM (6 years, 5 months ago)

Got it, thank you!


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OfflineDA Double G
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Re: Pastywhyte's Easy Agar Tek [Re: nwcleaningllc]
    #24547280 - 08/11/17 11:05 PM (6 years, 5 months ago)

I made a batch up last night, but I did it just the minimum amount of heat I thought was necessary to reduce condensation. Just made some x-fers and it looks like there was a little bit of settling. Like the bottom portion of the plate was a bit different color. Surely the 45 min PC run would get all the nutes in suspension ? I used powdered agar and just stirred on low till the potato flakes dissolved.


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Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: DA Double G]
    #24547286 - 08/11/17 11:07 PM (6 years, 5 months ago)

That's normal. Some solids will settle. It's not a problem.


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OfflineDA Double G
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24547356 - 08/11/17 11:43 PM (6 years, 5 months ago)

Ok thank you, this is also the first time I've made a batch with purple coloring. The darker colors are making things stand out alot better. Which is really great. Love this tek!


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Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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InvisibleComebackKid
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Re: Pastywhyte's Easy Agar Tek [Re: DA Double G]
    #24547373 - 08/11/17 11:50 PM (6 years, 5 months ago)

I usually dye my agar different colours to remember what ingredients I used but, for that very reason, blue has to be my favourite by far so I ended up using both a light blue and a dark blue :lol:


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

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OfflineDA Double G
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Re: Pastywhyte's Easy Agar Tek [Re: ComebackKid]
    #24547471 - 08/12/17 01:03 AM (6 years, 5 months ago)

I've been thinking about a color coded system myself. Thought it could make things simpler and reduce a little labeling.
So far my favorite is also blue, I tried green early on with a drop of spore solution, but I must've done something wrong on those cause they never did anything. Its a weird thing there. It was the first syringe I bought from a non-sponsored vendor. It kinda worked ok for pf jars, but I couldn't get it to do anything on agar. And most of the pf jars contaminated right at the end.
Anyway im gonna give the green a go next time I do a transfer. Gonna see how this purple turns out, the ones I did tonight are second transfer from cloned tissue.
Sad thing is, im off tomorrow and really don't have anything to do.
I usually spend the weekend prepping grains, but ive run out of jars.
The struggle
Guess ill work on strain isolation


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Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.


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OfflineBreakTheSOB
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Re: Pastywhyte's Easy Agar Tek [Re: DA Double G]
    #24554993 - 08/15/17 04:44 AM (6 years, 5 months ago)

I do a different color each run as well, mixed yellow and red to make orange last time red and blue for purple yesterday, then I slide everything to the right, newer ones on the left, I fucked up a batch by not heating it enough and it was really really soft even after pc, was able to get myc off of it tho to some good ones, I started 5 weeks ago and have done hundreds of transfers on T5 now which is the sixth set of plates.

I use plastic shot cups from dollar tree can do 20 to 27 per 23 qt presto run. If one is beautiful I put it on a bigger pasty plate. No excuse not to start with agar. You'll have so much shit going on it will keep u busy as fuck but you'll have healthy myc

If you are a first timer I would suggest sterilizing some cotton swabs, since you don't know how to use a syringe yet you can put a drop on it it then touch then center of your plate. I got multiple drops on my first ones and 2 developed sacchoromyces from all the water rolling around combined with ignorance and no technique, none since.

I go 1 g lme 1 gram agar per each 50 ml, sometimes .8 grams lme per another tek can't remember his name, I make 200 to 250 ml batches because I can fit more due to using smaller plates


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"A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle


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OfflineLennybernadino
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Re: Pastywhyte's Easy Agar Tek [Re: BreakTheSOB]
    #24555180 - 08/15/17 07:52 AM (6 years, 5 months ago)

Apparently food coloring is not easily available in Poland, So I tried Beet juice agar, that did not work the color turns to dark brown in the PC , still good agar but not the color I wanted though it is still dark.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Lennybernadino]
    #24555262 - 08/15/17 08:55 AM (6 years, 5 months ago)

SarahTonin had some good results with beet agar.


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OfflineBreakTheSOB
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Re: Pastywhyte's Easy Agar Tek [Re: Lennybernadino]
    #24555455 - 08/15/17 10:24 AM (6 years, 5 months ago)

probably due to the sugars


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"A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle


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OfflineWalkinonsunshine
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Re: Pastywhyte's Easy Agar Tek [Re: BreakTheSOB]
    #24565432 - 08/19/17 03:33 PM (6 years, 5 months ago)

I'm just wondering if a drop from a flamed ms syringe through the micropore tape onto the agar below would be a viable technique for this tek within my SAB?

my original plan is a PC'ed swab of a spore print, swab the agar, then put the lid back on. The syringes are just different strains I have, and are not planning on being my most used tek.

I hope my noobness isn't clouding the gist of the question. I'm basically trying to use the ms syringe to the agar in the cleanest possible way. I'm probably over stressing the situation since I'm using a SAB anyway.


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OfflineSteevo
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Re: Pastywhyte's Easy Agar Tek [Re: Walkinonsunshine]
    #24565658 - 08/19/17 05:09 PM (6 years, 5 months ago)

You are and that's cool but you could inoculate through the mp tape like you said. Just have another strip ready and cover the hole as you pull the needle out


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OfflineLennybernadino
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24566859 - 08/20/17 08:22 AM (6 years, 5 months ago)

Quote:

Pastywhyte said:
SarahTonin had some good results with beet agar.



It is a good agar, Myc loves it but it is NOT red!


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Lennybernadino]
    #24566860 - 08/20/17 08:23 AM (6 years, 5 months ago)

Indeed. I believe hers was a golden brown.


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Offlineblackout
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Re: Pastywhyte's Easy Agar Tek [Re: Walkinonsunshine]
    #24567252 - 08/20/17 11:39 AM (6 years, 5 months ago)

Quote:

Walkinonsunshine said:
I'm just wondering if a drop from a flamed ms syringe through the micropore tape onto the agar below would be a viable technique for this tek within my SAB?



Yeah, you will need more tape as Steevo said. If flaming and only doing 1 drop I usually flame and squirt a little to cool the needle -rather than let it cool in the air.

I have SHIPs on some of my plates, i.e. a hole in the centre of the lid with silicone. I put in spare needles while in the PC, this allows me to have the lid on tight and no filter on the container. After cooling I remove the plate and pull out the needle. This allow me to be more careful and exact to inject a drop directly in the centre of the plate.

Some do not like the idea of SHIPs as the surface might be contaminated, but you can cover them with foil before going in the PC.

As you are working with agar many presume the syringe to be contaminated anyway, and will be transferring away from the first plate.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: blackout]
    #24567995 - 08/20/17 06:04 PM (6 years, 5 months ago)

IMO fuck syringe to agar, unless you are having trouble hydrating spores then stick to print. if you must use it squirt some onto a sterile swab and swipe it. better yet, make a couple cakes and take a clone to agar if you want the genetics in your syringe


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
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OfflineWalkinonsunshine
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #24568070 - 08/20/17 06:40 PM (6 years, 5 months ago)

Quote:

blackout said:
I have SHIPs on some of my plates, i.e. a hole in the centre of the lid with silicone. I put in spare needles while in the PC, this allows me to have the lid on tight and no filter on the container. After cooling I remove the plate and pull out the needle. This allow me to be more careful and exact to inject a drop directly in the centre of the plate.

Some do not like the idea of SHIPs as the surface might be contaminated, but you can cover them with foil before going in the PC.

As you are working with agar many presume the syringe to be contaminated anyway, and will be transferring away from the first plate.





So let me get this straight. You are placing one of your needles into the self-healing injection port silicone at the center of your Petrie round. Then you are autoclaving it, then once the autoclaving is finished you take out the syringe? I'm assuming that the hole in the needle offers gas exchange much like the whole with tape on it would?

You're right with transferring. That is certainly my intent.

Quote:

tryptkaloids said:
IMO fuck syringe to agar, unless you are having trouble hydrating spores then stick to print. if you must use it squirt some onto a sterile swab and swipe it. better yet, make a couple cakes and take a clone to agar if you want the genetics in your syringe




Hmm. I'm not totally into the genetics, I just have a few syringes of other types that I want to use. My primary intent is to use dry prints and sterile swabs like in Pasty's original. I was just looking to get the syringes out, get a specimen going and see if it is good to go. If it is, great and I can grow a batch out, toss the syringes and use spore prints for long term and tissue samples for cloning, if amazing results ensued. (I doubt that will happen soon.)

I just want to make it clear I'm not interested in mucking about with this tek, I'm just looking to use up syringes as an alternate method until my syringes are gone.  Seemed like a slam dunk to go through a membrane with a needle.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: Walkinonsunshine]
    #24568105 - 08/20/17 06:53 PM (6 years, 5 months ago)

as said before you can as long as you re apply micropore. but if you are just trying to get rid of syringes I suggest pf tek. it takes very little to inoculate agar.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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