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Invisiblejkz
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I'm a teapot User Gallery


Registered: 06/16/13
Posts: 1,910
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #24309980 - 05/10/17 02:25 PM (6 years, 8 months ago)

Quote:

Pastywhyte said:
The grocery store. $4 is the high price. A couple months ago they had them on sale for $2.50 and I loaded up. Might even be cheaper in the U.S. We seem to get hosed on prices for everything up here.




We're always getting fucked one way or another.
Also, I gotta work at keeping the condensation to a min. Might go prep some more "plates" after and try what cronicr said a bunch of pages back about letting them reach room temp before PCing.
I've also been using old PP5 containers I had for cakes back in the day. With the 4 holes on the sides. I just double up on the micropore tape.


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Offlineesse_jeremy
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Re: Pastywhyte's Easy Agar Tek [Re: jkz]
    #24312420 - 05/11/17 04:45 PM (6 years, 8 months ago)

Any advice to keep the condensation to min???

The only one I know is -dont open the pc before the overnight cooling down process

but I always have to open it because I need to know if the pile of plates is laying on the side (which will cause the agar to become solid not flat)


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
    #24312558 - 05/11/17 05:50 PM (6 years, 8 months ago)

let them cool before loading into the
pc, also you can bring it up to pressure and lower the pressure slowly. that should help


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleNothingsChanged
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Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
    #24313415 - 05/12/17 02:00 AM (6 years, 8 months ago)

Quote:

esse_jeremy said:
Any advice to keep the condensation to min???

The only one I know is -dont open the pc before the overnight cooling down process

but I always have to open it because I need to know if the pile of plates is laying on the side (which will cause the agar to become solid not flat)



Put empty jars in with them to fill the space. You shouldn't be worrying about such things.(Things tipping over)


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OfflineMyco.stoner97
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Registered: 05/18/17
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Last seen: 6 years, 5 months
Re: Pasty Agar Tek [Re: NothingsChanged]
    #24329025 - 05/18/17 04:32 AM (6 years, 8 months ago)

Hey new to this..do i add water to the pc? Maybe stupid question but i dont wanna fuck up lol..


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OfflineJesh
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Re: Pasty Agar Tek [Re: Myco.stoner97]
    #24329093 - 05/18/17 06:11 AM (6 years, 8 months ago)

Yes, you PC normally so that would involve using water


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Jesh]
    #24329785 - 05/18/17 11:31 AM (6 years, 8 months ago)

2 or 3 inches unless you want to burn plastic and warp your pc


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisiblebodhisattaMDiscordReddit
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Re: Pasty Agar Tek [Re: Myco.stoner97]
    #24329804 - 05/18/17 11:39 AM (6 years, 8 months ago)

Quote:

Myco.stoner97 said:
Hey new to this..do i add water to the pc? Maybe stupid question but i dont wanna fuck up lol..



read your PC's user manual.......

also,
https://www.shroomery.org/forums/showflat.php/Number/24180711/vc/1#24180711


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OfflineOrganic_Magic
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Re: Pasty Agar Tek [Re: bodhisatta]
    #24329909 - 05/18/17 12:20 PM (6 years, 8 months ago)

Pressure cookers are like women, you gotta give em a few inches at least or they just won't work right for you.:shrug:


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OfflineBodhiBoy
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Re: Pasty Agar Tek [Re: Organic_Magic]
    #24343987 - 05/23/17 06:36 PM (6 years, 8 months ago)

So i searched all over town for the exact brands used here and have come short on the tupperware believe it or not and have only found this micropore tape and this agar. Will these be fine? It says micropore tape on the back but i saw another post where somone mentioned this product isnt the right stuff.



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InvisiblePipefitter537
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Re: Pasty Agar Tek [Re: BodhiBoy]
    #24344008 - 05/23/17 06:46 PM (6 years, 8 months ago)

Yup that will work. Any little container that has a 5 in the recycling triangle will work


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InvisibleJHOVA
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Re: Pasty Agar Tek [Re: Pipefitter537]
    #24344380 - 05/23/17 08:51 PM (6 years, 8 months ago)

will lack of air cause bacterial growth while making spore prints? All my agar came out hagard.



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OfflinePsilosoulful

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Re: Pasty Agar Tek [Re: JHOVA]
    #24344387 - 05/23/17 08:53 PM (6 years, 8 months ago)

I think I see one spec of clean mycelium, transfer that out to another clean agar dish.
Your bacterial issues were likely caused by lack of sterile procedures or you just didn't PC them long enough


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Invisiblejkz
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Registered: 06/16/13
Posts: 1,910
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Re: Pasty Agar Tek [Re: Psilosoulful]
    #24344406 - 05/23/17 08:59 PM (6 years, 8 months ago)

:scarybeat:


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InvisibleJosex
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Re: Pasty Agar Tek [Re: JHOVA]
    #24344987 - 05/24/17 03:11 AM (6 years, 8 months ago)

Quote:

Jonhova said:
will lack of air cause bacterial growth while making spore prints? All my agar came out hagard.





You gotta trash that shit, no second thoughts. I'd personally pc those plates before you open them next time you use your cooker.
The green is sporulating mold, it has dropped nasty spores all over the plate already. Even if you had cube myc in there it would be covered in nasty mold spores and you don't want to fuck with that.

Lack of air doesn't have anything to do, it does not cause contams and an agar culture can perfectly thrive and grow in a plate without any gas exchange whatsoever, just the little air that's in the plate is enough. We make holes in the containers so the pc won't crush them. Probably your inoculum was the culprit, or your technique, or both.


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OfflineExplicitdetail
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Re: Pasty Agar Tek [Re: Josex]
    #24345745 - 05/24/17 10:40 AM (6 years, 8 months ago)

Okay so here a question for you guys, I recently found this food coloring gel



Ingredients: Betty Crocker™ Neon Gel Food Colors.
Corn syrup, sugar, water, modified cornstarch, yellow 5, yellow 6, red 3, carrageenan, potassium sorbate and sodium benzoate (preservatives), blue 1, citric acid.

Do you think since this already has corn syrup and sugar added I should skip the karo/honey, or scratch the idea and just use the traditional coloring. Thanks in advance!


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InvisibleJosex
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Re: Pasty Agar Tek [Re: Explicitdetail]
    #24345771 - 05/24/17 10:48 AM (6 years, 8 months ago)

Depends on how much of the stuff is needed to color your agar.  If one drop is enough to color a cup of water then it won't really affect anything and you should proceed with the prep as usual. Don't know jack about food color in gel form but I guess you'd only need to add very little of the stuff, as it happens with most if not all food colors. Stuff is really concentrated, they're made to color your food, not to add any real nutritional value or flavor.


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OfflineExplicitdetail
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Re: Pasty Agar Tek [Re: Josex]
    #24345794 - 05/24/17 11:04 AM (6 years, 8 months ago)

Quote:

Josex said:
Depends on how much of the stuff is needed to color your agar.  If one drop is enough to color a cup of water then it won't really affect anything and you should proceed with the prep as usual. Don't know jack about food color in gel form but I guess you'd only need to add very little of the stuff, as it happens with most if not all food colors. Stuff is really concentrated, they're made to color your food, not to add any real nutritional value or flavor.




I made a batch with it last night and only used a small drop on a spoon and it did the trick, I just wasn't sure if the added sugar would effect it.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Explicitdetail]
    #24346070 - 05/24/17 01:03 PM (6 years, 8 months ago)

food coloring is almost useless


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemushtard
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Registered: 11/29/16
Posts: 61
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24356212 - 05/28/17 11:05 AM (6 years, 7 months ago)

I have the eden agar I got at whole foods and grain water from oats. 
What are the grain water ratios, did a search and didnt find solid numbewrs


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