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OfflineBry Bry
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24063037 - 02/03/17 04:18 PM (6 years, 11 months ago)

Poured my first plates today. Had a bitch of a time finding agar locally so off to Amazon I went. Got 500 grams of powdered agar for around $25 shipped.

It's killing me waiting on the plates to cool so I can start with the spore syringes I got from from a forum sponser Gives me a good reason to try the new gloves on my glove box.


Edited by Bry Bry (02/03/17 05:29 PM)


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OfflineAK1000
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Re: Pasty Agar Tek [Re: Bry Bry]
    #24063137 - 02/03/17 04:56 PM (6 years, 11 months ago)

Quote:

Bry Bry said:
Poured my first plates today. Had a bitch of a time finding agar locally so off to Amazon I went. Got 500 grams of powdered agar for around $25 shipped.

It's killing me waiting on the plates to cool so I can start some with the spore syringes I got from from Mushrooms.com. Gives me a good reason to try the new gloves on my glove box.




I thought glove boxes were more sketchy than SAB's!


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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OfflineBry Bry
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Re: Pasty Agar Tek [Re: AK1000]
    #24063190 - 02/03/17 05:10 PM (6 years, 11 months ago)

Dunno. I'm new at this but everything gets the shit whipped off of it with Lysol and/alcohol. If it's badass enough to beat through that then it can have the damn plate.

Until I get a flow hood built, it'll have to do.


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: Bry Bry]
    #24063417 - 02/03/17 06:45 PM (6 years, 11 months ago)

Quote:

AK1000 said:
Quote:

Bry Bry said:
Poured my first plates today. Had a bitch of a time finding agar locally so off to Amazon I went. Got 500 grams of powdered agar for around $25 shipped.

It's killing me waiting on the plates to cool so I can start some with the spore syringes I got from from Mushrooms.com. Gives me a good reason to try the new gloves on my glove box.




I thought glove boxes were more sketchy than SAB's!



ummm... no. exact opposite. woops
sabs>glovebox

Quote:

Bry Bry said:
Dunno. I'm new at this but everything gets the shit whipped off of it with Lysol and/alcohol. If it's badass enough to beat through that then it can have the damn plate.

Until I get a flow hood built, it'll have to do.



you only need water and a drop of dishsoap in a SAB. Lysol is pointless and alcohol will make it perform worse and make it dangerous as shit. a SAB that has been saturated with alcohol is a bomb waiting to blow up in your face, literally. we have had users goto the hospital because their SAB blew up from using alcohol on it.

It is fine to use alcohol-soaked paper towels inside your sab to clean plates, and you can use it to clean the box between sessions. but the ONLY way to prepare a SAB is to apply soapy water to the walls and LEAVE IT. the walls should always be wet with soapy water, not lysol, and definitely not alchol.


Edited by Munchauzen (02/03/17 07:12 PM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24063440 - 02/03/17 06:55 PM (6 years, 11 months ago)

Quote:

Munchauzen said:
Quote:

AK1000 said:
Quote:

Bry Bry said:
Poured my first plates today. Had a bitch of a time finding agar locally so off to Amazon I went. Got 500 grams of powdered agar for around $25 shipped.

It's killing me waiting on the plates to cool so I can start some with the spore syringes I got from from Mushrooms.com. Gives me a good reason to try the new gloves on my glove box.




I thought glove boxes were more sketchy than SAB's!



ummm... no. exact opposite.





I would agree if it were a properly made glovebox but anything made in a tote will add unnecessary drafts


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24063464 - 02/03/17 07:11 PM (6 years, 11 months ago)

i actually misread that. i thought he was saying gloveboxes are better than sabs. my bad. been a long day.

a lab glovebox is obv the best. but in amatuer mycology, you cannot build a homemade glovebox that will be as effective as a sab.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24063479 - 02/03/17 07:25 PM (6 years, 11 months ago)

people build flowhoods :shrug: it's possible it just depends on how you do it. I wouldn't recommend it to anyone though so the point is moot


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24063489 - 02/03/17 07:29 PM (6 years, 11 months ago)

I'm not sure what you are trying to say. Of course people build flowhoods. We aren't talking about flowhoods, though.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24063500 - 02/03/17 07:34 PM (6 years, 11 months ago)

I'm trying to say that if someone really wanted to they would be able to build a lab grade glovebox.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: tryptkaloids] * 2
    #24063564 - 02/03/17 08:09 PM (6 years, 11 months ago)

eh, no. they tig weld stainless steel in cleanrooms to make them.


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OfflineBry Bry
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24063636 - 02/03/17 08:50 PM (6 years, 11 months ago)

Soap water it is then, Thanks!


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OfflineMarmie
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Re: Pasty Agar Tek [Re: Bry Bry]
    #24063705 - 02/03/17 09:19 PM (6 years, 11 months ago)

so I got into agar about a month ago thanks to pasty plates, I have a few clones on agar, these are 3rd transfer,


do they look alright, I was thinking of looping a few pieces to new plates then tiger dropping whats left to a few rye quarts in a day or 2, should I just do it now or should I wait until the plate is almost fully conlonized. any imput would be much appreciated thanks in advance.


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OfflineKenetic
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Re: Pasty Agar Tek [Re: Bry Bry]
    #24063725 - 02/03/17 09:27 PM (6 years, 11 months ago)

get good enough and you don't need to spray shit!

iso soaked paper towel for wiping plates is all I use now. 

I helped build a bunch of cleanrooms for a few months and I have to say they are amazing.  The entire ceiling had the standard 2 by 4 grid but instead of tiles each cell had a hepa filter and dedicated blower.  Can you imagine an entire ceiling made of hepa filters?  Slits in the bottom of the walls allowed the air to escape and created positive pressure......what I would give to own one of those rooms.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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OfflineKenetic
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Re: Pasty Agar Tek [Re: Kenetic]
    #24063736 - 02/03/17 09:30 PM (6 years, 11 months ago)

Looks pretty good!  Nice work! 

I think you could do it now (after a little more colonization) or on the next transfer.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



DMT said: Everyone know's me, they just don't know it yet


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OfflineMarmie
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Re: Pasty Agar Tek [Re: Kenetic]
    #24063750 - 02/03/17 09:34 PM (6 years, 11 months ago)

Awsum thanks , ya i was just guna save the next  transfers for masters to keep expanding it .  They look clean as far as i can tell , ill guess ill find out for sure in a few days


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24065112 - 02/04/17 01:15 PM (6 years, 11 months ago)

Quote:

Munchauzen said:
eh, no. they tig weld stainless steel in cleanrooms to make them.



:rocketcrotch: damn, I guess you'd need the hookup


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineTiamo
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Re: Pasty Agar Tek [Re: Kenetic]
    #24065136 - 02/04/17 01:28 PM (6 years, 11 months ago)

Quote:

kenetic said:
I helped build a bunch of cleanrooms for a few months and I have to say they are amazing.  The entire ceiling had the standard 2 by 4 grid but instead of tiles each cell had a hepa filter and dedicated blower.  Can you imagine an entire ceiling made of hepa filters?  Slits in the bottom of the walls allowed the air to escape and created positive pressure




I would be scared to walk into the room, knowing that the shit coming off of me would contaminate it immediately. I would put on a big ass tyvek suit and mask.


--------------------


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Tiamo]
    #24065150 - 02/04/17 01:35 PM (6 years, 11 months ago)

Quote:

Tiamo said:
Quote:

kenetic said:
I helped build a bunch of cleanrooms for a few months and I have to say they are amazing.  The entire ceiling had the standard 2 by 4 grid but instead of tiles each cell had a hepa filter and dedicated blower.  Can you imagine an entire ceiling made of hepa filters?  Slits in the bottom of the walls allowed the air to escape and created positive pressure




I would be scared to walk into the room, knowing that the shit coming off of me would contaminate it immediately. I would put on a big ass tyvek suit and mask.



I'm pretty sure that's standard procedure


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineichugwindex
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24112963 - 02/23/17 09:04 AM (6 years, 11 months ago)

Ive tried this like 5 times now without success.

1. For some reason this recipe is only making me 5 plates?

2. Nothing seems to grow on them. Ive tried tissue samples and drop of LC and neither showed any visible changes

Any help? Sure would hate for this big ass bag of oats to go to waste.


--------------------
Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.


Edited by ichugwindex (02/23/17 09:06 AM)


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OfflineAK1000
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Re: Pasty Agar Tek [Re: ichugwindex]
    #24113109 - 02/23/17 10:08 AM (6 years, 11 months ago)

Quote:

ichugwindex said:
Ive tried this like 5 times now without success.

1. For some reason this recipe is only making me 5 plates?

2. Nothing seems to grow on them. Ive tried tissue samples and drop of LC and neither showed any visible changes

Any help? Sure would hate for this big ass bag of oats to go to waste.




Why would oats be going to waste if this is a thread about agar? If you're only able to make 5 plates, it sounds like too much liquid is evaporating and it's becoming extremely stiff/denser than optimal.  A good trick is to follow the recipe here by Munchauzen and for that recipe specifically notice he says "Upon boiling, immediately remove and pour into a measuring cup. You can use the the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in."


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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