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OfflineThe Mycologist
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Registered: 05/06/16
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Re: Pasty Agar Tek [Re: ComebackKid]
    #23999773 - 01/10/17 10:38 PM (7 years, 19 days ago)

oh, makes sense


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Pasty Agar Tek [Re: FunGuyZ]
    #24001828 - 01/11/17 05:37 PM (7 years, 18 days ago)

Quote:

FunGuyZ said:
Quote:

tryptkaloids said:
I think he boiled some water out and didn't have 8 plates worth




This is correct. Having trouble with the ratio for my particular brand of agar. My pasty plates are done now though. I just harvested my fruits and took some tissue samples. Not feeling too good about it- cut my finger making holes for my still air box- and didn't feel very comfortable inside it. First time so I can only improve.



I've always preferred using a large metal coffee can to melt the holes in... makes perfectly round holes with nothing to snag yourself on. larger holes can also make it more comfortable to work inside. also, your arm holes should be spaced the same distance as your shoulders. If it isn't, you put a lot of stress on your arms trying to hold them in position.

I've also always preferred to clone pins over cloning mature fruits, but I've done both.


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OfflineMycoMitch666
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Registered: 12/07/16
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Re: Pasty Agar Tek [Re: Munchauzen]
    #24017409 - 01/17/17 01:36 PM (7 years, 12 days ago)

Anybody have any suggestions on containers for agar sterilization?:rolleyes:


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:mushroom2:A closed mind is a good thing to lose:mushroom2:


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InvisiblemushboyMDiscord
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Re: Pasty Agar Tek [Re: MycoMitch666]
    #24017420 - 01/17/17 01:39 PM (7 years, 12 days ago)

the ones outlined in this tek?

any plastic rated at pp5(look on for the recycling logo with a '5')

anything glass.


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Offlineenlightenment
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Re: Pasty Agar Tek [Re: mushboy]
    #24017428 - 01/17/17 01:42 PM (7 years, 12 days ago)

:whathesaid:

This thread is about pre-poured agar plates. Look at the first page.

If you want to pour petri dishes:


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Offlinelovelaughlibs
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Registered: 10/14/15
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Re: Pasty Agar Tek [Re: enlightenment]
    #24017525 - 01/17/17 02:11 PM (7 years, 12 days ago)

Quote:

enlightenment said:
:whathesaid:

This thread is about pre-poured agar plates. Look at the first page.

If you want to pour petri dishes:




Yeah these bottles are great for pouring, if you wanna find them on ebay or amazon or whatever they're called media bottles, mine is a pyrex/borosilicate one :smile:


--------------------
Ask and ye shall receive; Seek and ye shall find.


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OfflineTiamo
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Re: Pasty Agar Tek [Re: lovelaughlibs]
    #24017552 - 01/17/17 02:21 PM (7 years, 12 days ago)

Also known as reagent bottles.


--------------------


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InvisiblebodhisattaMDiscordReddit
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Re: Pasty Agar Tek [Re: Tiamo] * 2
    #24017569 - 01/17/17 02:26 PM (7 years, 12 days ago)



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InvisibleIntelligentxfruit
Earth Hippy


Registered: 01/06/13
Posts: 1,545
Re: Pasty Agar Tek [Re: bodhisatta]
    #24017615 - 01/17/17 02:44 PM (7 years, 12 days ago)

I pour with these

into


baby stuff makes great for agar:thumbup:


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OfflineKenetic
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Re: Pasty Agar Tek [Re: bodhisatta]
    #24017640 - 01/17/17 02:52 PM (7 years, 12 days ago)

Quote:

bodhisatta said:





Damn right they are.


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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OfflineMycoMitch666
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Registered: 12/07/16
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Re: Pasty Agar Tek [Re: Kenetic]
    #24017796 - 01/17/17 04:00 PM (7 years, 12 days ago)

Sweet! Thanks guys:thumbup: I will definitely be buying a few of these reagent bottles tonight :typing:


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:mushroom2:A closed mind is a good thing to lose:mushroom2:


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pasty Agar Tek [Re: MycoMitch666]
    #24019460 - 01/18/17 09:38 AM (7 years, 12 days ago)

.


Edited by Thoky.ykoht (04/18/17 07:49 AM)


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OfflineAK1000
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Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #24019622 - 01/18/17 10:44 AM (7 years, 12 days ago)

Honestly the slant is okay and it'll colonize just fine so you don't really need to mess with it. But if you really want to and have a microwave you could prolly blast em with microwaves for a few seconds and they'll become liquid and recenter.


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pasty Agar Tek [Re: AK1000]
    #24019648 - 01/18/17 10:56 AM (7 years, 12 days ago)

.


Edited by Thoky.ykoht (04/18/17 07:49 AM)


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InvisiblemushboyMDiscord
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Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #24019894 - 01/18/17 12:51 PM (7 years, 11 days ago)

how much media did you use per glad?
this is what mine looked like :shrug:

first pastyplates i ever made :datass:


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InvisibleAdden
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Registered: 06/04/03
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Loc: Flag
Re: Pasty Agar Tek [Re: mushboy]
    #24020997 - 01/18/17 06:52 PM (7 years, 11 days ago)

2cm maybe

Well, depending what you're doing.

I like to fill the minis right to that line where they start to widen out. The pint ones there's a kind of identifiable line on the ones I get.


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Offlineblackout
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Re: Pasty Agar Tek [Re: AK1000]
    #24023145 - 01/19/17 02:53 PM (7 years, 10 days ago)

Quote:

AK1000 said:
But if you really want to and have a microwave you could prolly blast em with microwaves for a few seconds and they'll become liquid and recenter.



yep. a microwave will usually melt the agar in a localised area first, this can be enough to swirl around and mix with any "free water" and make it all set again. If you microwave to the stage where it is all liquid you will probably have condensation problems again.


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InvisibleMoabfighter
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Re: Pasty Agar Tek [Re: blackout]
    #24023855 - 01/19/17 07:11 PM (7 years, 10 days ago)

Need to make some plates for masters.

Anyone got a simple recipe for master plates? I'm thinking maybe just a little less agar powder, and a little less nutes, to get rhizomorphic growth and maybe not as stiff agar for easy breaking up in the grains. Cutting, slicing, somewhere im getting contams. I'd like to make 6 masters off a 5th transfer plate, grow those out, and use one plate per jar x6 jars.

Seem feasible?


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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: Moabfighter]
    #24027828 - 01/21/17 10:32 AM (7 years, 9 days ago)

I think they call that one the 'tiger drop'.


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Moabfighter]
    #24028124 - 01/21/17 12:28 PM (7 years, 9 days ago)

Quote:

Moabfighter said:
Need to make some plates for masters.

Anyone got a simple recipe for master plates? I'm thinking maybe just a little less agar powder, and a little less nutes, to get rhizomorphic growth and maybe not as stiff agar for easy breaking up in the grains. Cutting, slicing, somewhere im getting contams. I'd like to make 6 masters off a 5th transfer plate, grow those out, and use one plate per jar x6 jars.

Seem feasible?



a master plate is one you go back to for reuse. on the fifth transfer I would take 2, keep one in the fridge for later use and make 6 transfers off the other one and tiger drop them


--------------------
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