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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: no0bsawse]
    #23933366 - 12/16/16 07:13 PM (7 years, 1 month ago)

minerals should be settling, not floating. make a test batch with your tap and one with bottled to see it it makes a difference. do you drink your tap water?


--------------------
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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #23933487 - 12/16/16 07:47 PM (7 years, 1 month ago)

Minerals are good for mushrooms.  What you're describing isn't what I'd call a problem.


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #23933695 - 12/16/16 09:01 PM (7 years, 1 month ago)

if minerals are being separated out of his water wouldn't they settle? if you drink it your mushrooms will do fine. if it's simply bad water then you may want to watch out for heavy metals and such
what do I know :shrug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids] * 1
    #23933711 - 12/16/16 09:07 PM (7 years, 1 month ago)

What are saying you?  I thoughts everybody likesed the heavy metals.



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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #23933801 - 12/16/16 09:53 PM (7 years, 1 month ago)

lol nice, I wouldn't want to use flint water on anything though


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineHyper
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Re: Pasty Agar Tek [Re: no0bsawse]
    #23933823 - 12/16/16 10:00 PM (7 years, 1 month ago)

I'm a little confused by the Agar method.

Is the point of it to create living mycelium and then to use that myc in a separate substrate?

if so, how do you transfer it from one to the other?

is there a substrate that this works best with? It seems like brown rice flower cakes generally have lower yields


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Hyper]
    #23933843 - 12/16/16 10:10 PM (7 years, 1 month ago)

the point is to be able to ensure your inoculate is clean so we don't waste grain spawn and substrate. it also helps save on spore usage. we use a scalpel or x-acto knife to transfer clean mycelium to an empty plate. and then when we have a clean culture we can inoculate grain.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #23933847 - 12/16/16 10:12 PM (7 years, 1 month ago)

read this https://www.shroomery.org/forums/showflat.php/Number/21889950 the only thing I would change is hold your plates upside down to make sure you don't put anything over the agar


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: Hyper]
    #23934048 - 12/16/16 11:55 PM (7 years, 1 month ago)

Okay I'm drunk so forgivery NE but I'm a try and explain this shit

Okay agar is like jello
You mix it aa liquid and then it hardens
When you drop spores onto it they germinate and grow mycelium
Mycelium can't penitrate the agar so it grows along the surface
The bacteria and mycr grow on the agar on a 2d surface
When you see mycelium growing on its own without bacteria then you cut that piece of agar out of the surface and move it to another plate of agar.
You may have transfered bacteria by accident but it will be less prominent than the last plate so it won't grow as fast as the good mycelium
Then you cut out the good clean mycelium from that plate and transfer to another plate. Repeat this process until you have nothing but clean mycelium growth.
Then transfer that to grain spawn or make )LC.
You will finally have clean mycelium for your grows


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OfflineKenetic
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Re: Pasty Agar Tek [Re: ComebackKid]
    #23934950 - 12/17/16 10:09 AM (7 years, 1 month ago)

And we use agar for cloning and isolating.


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Re: Pasty Agar Tek [Re: Kenetic]
    #23934987 - 12/17/16 10:20 AM (7 years, 1 month ago)

And mycelium can penetrate the agar, just use and inoc loop to "bury" some spores in the agar and watch what happens, just for fun. It's bacteria that can't travel through agar, that's the whole concept of the sandwich tek btw.


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Re: Pasty Agar Tek [Re: Josex]
    #23937415 - 12/18/16 08:34 AM (7 years, 1 month ago)

Quote:

Josex said:
And mycelium can penetrate the agar, just use and inoc loop to "bury" some spores in the agar and watch what happens, just for fun. It's bacteria that can't travel through agar, that's the whole concept of the sandwich tek btw.




Confirmed dude can read. :thumbup:


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Re: Pasty Agar Tek [Re: Inocuole]
    #23937605 - 12/18/16 10:10 AM (7 years, 1 month ago)

:wonka:
Thanks :derfase:
But I actually went ahead and dipped the inoc loop in the agar accidentally and this happened

The pic doesn't show it very clearly but the myc is growing underneath the surface and 2 days after taking a wedge some fluffy myc started to resurface.


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Re: Pasty Agar Tek [Re: Josex]
    #23937612 - 12/18/16 10:13 AM (7 years, 1 month ago)

Observe the growth of the fluffy mycelium. It grew underneath and starts to reach the top of the plate. I would not worry too much by now.


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Re: Pasty Agar Tek [Re: enlightenment]
    #23937631 - 12/18/16 10:22 AM (7 years, 1 month ago)

I opened the plate in open air to take the pic and breathed on it for the fuck of it, so the only thing I'm going to be observing is all the nasty shit that'll grow on it, gonna keep it for shit and giggles.


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OfflineKenetic
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Re: Pasty Agar Tek [Re: Josex]
    #23937693 - 12/18/16 10:54 AM (7 years, 1 month ago)

I doubt youll be impressed


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Re: Pasty Agar Tek [Re: Kenetic]
    #23945500 - 12/21/16 09:12 AM (7 years, 1 month ago)

Just started my first agar venture. I read pasty's tek a few times over, and at the moment  I've got 10 petri's in the PC phase. I used Agar powder (no additives), potato flakes (no additives), RAW honey, and distilled water (just to be on the safe side). After going through the process of preparing the mixture, I've got a feew questions.

1. If I'm using petri dishes, should I still put a piece of paper towel on top of the lid? (I did this for 8/10 of the dishes, mostly because I forgot to put the paper towel for the first 2, but left them as they were just to see the difference).

2. Is there any length of (waiting) time between pouring and starting the PC phase? I only waited about 10 mins. I can't imagine it would make a difference since it's getting re-heated.

3. How long is the optimal time of heating the mixture? I know the "adding honey" phase is after the mixture is heated a bit, but how long should it stay on the burner on low? I kept it going for only about 2-4mins, while stirring constantly.

4. At what phase does it turn into the jelly-like texture? Does this happen while it cools in the PC?


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Re: Pasty Agar Tek [Re: maxpassin]
    #23945516 - 12/21/16 09:17 AM (7 years, 1 month ago)

Uhh... Most petri dishes don't survive the PC cycle. Are you using glass petris? If not, they'll probably melt. You will want to use this tek for pouring agar in petris: https://www.shroomery.org/forums/showflat.php/Number/21922023#21922023

You can't use them as no-pour anyway because the agar boils out.

1. Don't PC petris
2. No, doesn't matter.
3. Boil the water first to a hard boil, then add ingredients at once. Stir lightly for 5 mins.
4.
Quote:

Agar exhibits hysteresis, melting at 85 °C (358 K, 185 °F) and solidifying from 32–40 °C (305–313 K, 90–104 °F). This property lends a suitable balance between easy melting and good gel stability at relatively high temperatures.




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Re: Pasty Agar Tek [Re: Tiamo]
    #23945538 - 12/21/16 09:26 AM (7 years, 1 month ago)

If you are using plastic petri dishes you cannot use this tek. They will melt in the pressure cooker. This is a no pour method meant to be used with the glad mini rounds or jars. You will need to follow a pour tek for actual sleeves of plates.


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Re: Pasty Agar Tek [Re: Tiamo]
    #23945547 - 12/21/16 09:30 AM (7 years, 1 month ago)

Ah shit. They're plastic petris.. guess I fucked up. Can't wait to see the shit that's inside my PC right now -_-.

Thanks for the info and reference to the pour-tek. I'll be re-doing everything later today.


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