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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Pasty Agar Tek [Re: ghaleon]
#23746457 - 10/17/16 05:16 PM (7 years, 3 months ago) |
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You're fine. But youre probably cooking with the cooker waaaay too high if youre losing that much water in 45 mins and the roommates are complaining about noise.
You want to cook on the lowest possible temperature. Once your jiggler starts moving turn the temperature down. Keep turning it down untill your jiggler only jiggles a few times a minute
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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 just enough to keep it at the desired pressure. should barely make any noise or release any steam.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Bought another pack of rounds earlier. Gonna try again tomorrow.
-------------------- KSSS And PE WBS.
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ghaleon
Stranger
Registered: 10/12/16
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Last seen: 5 years, 6 months
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One of my pasty plates imploded slightly. Guess I used too much pressure. The other plates appear to be fine.
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blackout


Registered: 07/16/00
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Re: Pasty Agar Tek [Re: ghaleon]
#23749429 - 10/18/16 04:27 PM (7 years, 3 months ago) |
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Quote:
ghaleon said: One of my pasty plates imploded slightly. Guess I used too much pressure. The other plates appear to be fine.
more likely you cooled it down to fast. Did the imploded one have a smaller filter hole or more tightly packed if polyfil? or anything unusual to differentiate it from the others, e.g. filter was wet.
If it cools too fast it cannot draw in air fast enough through the filter and so could hot handle the vacuum and collapse. You can slow down cooling by lowering the power of the hob, down to a power level which cannot sustain pressure but just keeps it warmer to allow slower cooling down.
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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Re: Pasty Agar Tek [Re: blackout]
#23749493 - 10/18/16 04:48 PM (7 years, 3 months ago) |
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Could also be from ruining the integrity of the glad containers by flattening the bottoms
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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ghaleon
Stranger
Registered: 10/12/16
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Re: Pasty Agar Tek [Re: blackout]
#23749902 - 10/18/16 07:13 PM (7 years, 3 months ago) |
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Quote:
blackout said: more likely you cooled it down to fast. Did the imploded one have a smaller filter hole or more tightly packed if polyfil? or anything unusual to differentiate it from the others, e.g. filter was wet.
If it cools too fast it cannot draw in air fast enough through the filter and so could hot handle the vacuum and collapse. You can slow down cooling by lowering the power of the hob, down to a power level which cannot sustain pressure but just keeps it warmer to allow slower cooling down.
The paper towel on top of the container was wet (the other plates were dry). The one that imploded did have a really small hole and I put two micropore tapes on top.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: Pasty Agar Tek [Re: ghaleon]
#23751304 - 10/19/16 07:42 AM (7 years, 3 months ago) |
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Just made some more plates. Another 8 pack. Color is a nice aqua blue this time...one plate from the last pour looks like it got quite abit of Nutes... wrapping in foil now. Then gonna try real hard to bring the pressure up real slow...
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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You don't need to get too crazy about bringing the heat up slow, it's more important to cool it slow. If you are using a cheap thin walled PC I just like to be sure that I'm not adding the rounds to already boiling water etc.
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Moabfighter
Tam Fighter


Registered: 12/13/15
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Quote:
Pastywhyte said: You don't need to get too crazy about bringing the heat up slow, it's more important to cool it slow. If you are using a cheap thin walled PC I just like to be sure that I'm not adding the rounds to already boiling water etc.
I noticed when I put the lids on, I got condensation from I guess the warm agar in the containers. So I wiped that out with a paper towel. They're in the PC now on a big stove eye on 3-4 heat. Feeling good about these. A lot better than last time.
-------------------- KSSS And PE WBS.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Everytime we do something we should learn something as well
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Mattisfat
Learning


Registered: 01/20/16
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Loc: Newcastle, England
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xPTxHIPPIe
Inspired



Registered: 06/13/16
Posts: 273
Loc: Desert
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Just read the last few post and I have been making these for over a month now. Gonna try bringing the pressure down slowly and see if I get better results. Always seem to have a little condensation on my plates but doesn't cause me any problems and it clears up before the plate grows out.
I notice with ever fail I learn a thing or 2. Recently I learned to put more agar in my dish. Had some plates that were a little short this weekend. Strangely enough I think they are still growing.
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blackout


Registered: 07/16/00
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Last seen: 2 months, 24 days
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Re: Pasty Agar Tek [Re: ghaleon]
#23751751 - 10/19/16 11:33 AM (7 years, 3 months ago) |
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Quote:
ghaleon said: The one that imploded did have a really small hole and I put two micropore tapes on top.
When the filters are wet they will usually restrict the incoming air even more. If containers are deformed but not actually cracked up can sometimes repair them by boiling them in a pot of water on the stove or microwave, then reshape them when hot. Repaired ones might not have a tight seal anymore but some actually prefer being able to take the lids off easier in a SAB.
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Moabfighter
Tam Fighter


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Re: Pasty Agar Tek [Re: blackout]
#23751859 - 10/19/16 12:13 PM (7 years, 3 months ago) |
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Man. Still got condensation from hell. I cooked on 2 on the burner. After cycle, turned down to one. Waited half hour, then off.
Pressure cooker was cold to touch just now so I got one out. Looks like it rained all over the walls of the container. Damn. May try wiping it out in the SAB....
-------------------- KSSS And PE WBS.
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
Last seen: 3 years, 20 days
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Quote:
Moabfighter said: Man. Still got condensation from hell. I cooked on 2 on the burner. After cycle, turned down to one. Waited half hour, then off.
Pressure cooker was cold to touch just now so I got one out. Looks like it rained all over the walls of the container. Damn. May try wiping it out in the SAB....
ive seen people say to shake the plate a bit to get the water to run together then pour it out through a small gap in a SAB
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ComebackKid
Multispore Enthusiast



Registered: 05/27/16
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I wouldn't try wiping it out. Contamination central.
My advice would be to stop giving a fuck about the condensation  Took me a while but I've come to terms with ithe finally myself. It's not hurting anybody anyway. You'll see what you need to see
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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Edmunter
Mr



Registered: 05/01/13
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Just turn it upside down in the sab and open the lid a little
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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Re: Pasty Agar Tek [Re: Edmunter]
#23752311 - 10/19/16 03:18 PM (7 years, 3 months ago) |
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If there is any pooling on the agar you can tilt the container and use the liqiud to collect most of the condensation and then flip overin sab and quickly open and let the water spill onto the bottom of the sab.
Also I let my plates sit in a clean tote until I need them(usually a week or so later) and much of the condensation has already dissipated by then. Just make sure to wipe the containers down really well before doing any sterile work to prevent any contams have settled on them during that week or so.
In the end as said the condensation is not a problem(other than visibility) and should mostly clear up by the time you need to see the culture and take a transfer.
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Edited by tombosley8 (10/19/16 03:19 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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You guys should be working with your dishes upside down to begin with. Condensation is not even something worth thinking about. Just ignore it.
Quote:
should mostly clear up by the time you need to see the culture and take a transfer.
I don't think it even matters then. I'm not looking thru the side when I take transfers, the lid is off and I'm looking directly at it thru the sab.
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