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Invisibledankington
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Re: Pasty Agar Tek [Re: NumeroEno]
    #23663499 - 09/20/16 10:36 PM (7 years, 4 months ago)

yeah, mold you begin to read by the way the myc itself grows (or I have). It's pretty much the bane of my existence, but I've sure learned a lot from having it fuck with me so much.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: NumeroEno]
    #23663512 - 09/20/16 10:41 PM (7 years, 4 months ago)

The color is totally optional and like many things in the hobby has its positives and negatives. I agree that depending on how dark you make it and the color you choose, some mold species can be tougher to spot. But I like to think of this tek as the intro to agar for most. While I still prefer to do it to pour agar (for most stuff, sometimes pour agar can't be beat) I have no illusions that most people will use this tek to start and then move on.

Cam you believe that this thread has over 200K views? I am shocked. When I was being blackmailed into posting this I never imagined that it would take off to this level or get so many people into agar. I am humbled and only have the community to thank for their support and help maintaining this thread.

:seriousthankyou:


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InvisibleNumeroEno
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23663551 - 09/20/16 10:54 PM (7 years, 4 months ago)

Dude, this thread isn't even a sticky but it's been on the front page for the entire time I've been registered lol. Yeah that's why I said the food coloring is great for spotting bacteria. I highly recommend agar novices use it for that reason.


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:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisibledankington
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Re: Pasty Agar Tek [Re: NumeroEno]
    #23663597 - 09/20/16 11:07 PM (7 years, 4 months ago)

This tek is probably what kept me from throwing in the towel over a year ago. It's really amazing, but agar seems so intimidating at first. Then, you realize that you will fuck up, but everything you get right makes up for it. And you'll only get more and more right as time goes on.

Just gotta keep at it.


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OfflineLeftfield420
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Re: Pasty Agar Tek [Re: dankington]
    #23663644 - 09/20/16 11:19 PM (7 years, 4 months ago)

This was what got me into agar....I still use them when transfering sectors...use less agar with them than petris, not to mention they are perfect size to tiger drop into some sterile water to make LI....


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InvisibleNumeroEno
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Re: Pasty Agar Tek [Re: dankington]
    #23663649 - 09/20/16 11:20 PM (7 years, 4 months ago)

Seriously. I don't think I would have ever had the confidence to try agar if it weren't for this tek. I pour petris now but this is what got me started. Taking that first leap was the most significant event in my time cultivating mushrooms. I remember for my first attempt I couldn't find mini rounds so I used bigger containers and the agar was like 3/4" thick lol. But I was able to germinate spores and grow a mini mono from WBS inoculated with a wedge from that giant plate, and then it was like a whole new world opened up. Pic related.



--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: NumeroEno]
    #23663674 - 09/20/16 11:28 PM (7 years, 4 months ago)

Is that PEU? I always wanted to try PEU. Maybe next time I treat myself it will be to a PEU syringe.


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InvisibleSupalemonhaze
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Re: Pasty Agar Tek [Re: dankington]
    #23663903 - 09/21/16 12:59 AM (7 years, 4 months ago)

Quote:

dankington said:

Clear can be just as beneficial, if you know what you're looking for.




I agree. Personally, I love food colouring, I still use it every time. Actually, I use different colours for different recipes so I don't have to write down what type of agar is in any random plate. But if the agar is clear and transparent, it's even more see-through.

Generally with homemade agar I get yellowish agar that is somewhat cloudy so I prefer to colour it but some pre-mades are really clear, to the extent where colouring it only reduces visibility. I have this pre-made sabouraud agar that is the clearest agar I have seen so far, too bad the growth sucks balls on it but oh well.


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InvisibleNumeroEno
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23664320 - 09/21/16 07:55 AM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Is that PEU? I always wanted to try PEU. Maybe next time I treat myself it will be to a PEU syringe.




Yep. My first ever agar grow.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: stareatclouds]
    #23665710 - 09/21/16 05:01 PM (7 years, 4 months ago)

Quote:

stareatclouds said:
Quote:

Inocuole said:
The potato flakes scale with eveything else.. if shit is acting fucky just add more water.




Not sure why it was so cloudy. Weird. Hoping the agar was fine.




To follow up, I made some transfers of plates, flame sterilized and all that, and wound up with a bunch of contaminated shit. Like, horribly contaminated, worse than when I first tried agar. A few came out unscathed and are growing quickly so I don't know wtf is going on.

Is it possible I bought the wrong agar and that's why or would that not be it? It just seems like I couldn't have flicked that many mold spores or w/e when I'm not awful at agar. I'm talking like 5 big specs of green within like 1-2 days of the transfer.

The unused plates are still fine.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: stareatclouds]
    #23665715 - 09/21/16 05:02 PM (7 years, 4 months ago)

Sounds like bad technique to me.


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InvisibleComebackKid
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23665748 - 09/21/16 05:15 PM (7 years, 4 months ago)

Damn you just got told son!


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23666031 - 09/21/16 07:05 PM (7 years, 4 months ago)

Quote:

Pastywhyte said:
Sounds like bad technique to me.




I've never fucked up that bad. And the plates I took the transfers from aren't fucked up either, which is the weird part. Literally have never had this happen, heh.


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Invisibledankington
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Re: Pasty Agar Tek [Re: stareatclouds]
    #23666123 - 09/21/16 07:30 PM (7 years, 4 months ago)

Are the containers new? While they're still a bit tough to open, it's harder to keep the air still. I'm troubleshooting a similar situation atm. Pretty sure my SAB was also too small.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: dankington]
    #23666150 - 09/21/16 07:41 PM (7 years, 4 months ago)

Nah, old containers. The air was as still as it usually was. The only thing I can think of is that my first plates I transferred were the ones fucked the most. By far. Maybe my scalpel had shit on the handle and stuff? I donno. I'll try again later.


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OfflineTravelAgency
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23666859 - 09/21/16 11:58 PM (7 years, 4 months ago)

Quote:

Pastywhyte said:
The color is totally optional and like many things in the hobby has its positives and negatives. I agree that depending on how dark you make it and the color you choose, some mold species can be tougher to spot. But I like to think of this tek as the intro to agar for most. While I still prefer to do it to pour agar (for most stuff, sometimes pour agar can't be beat) I have no illusions that most people will use this tek to start and then move on.

Cam you believe that this thread has over 200K views? I am shocked. When I was being blackmailed into posting this I never imagined that it would take off to this level or get so many people into agar. I am humbled and only have the community to thank for their support and help maintaining this thread.

:seriousthankyou:




Thank you sir! Love all the information you provide us!

:leocheers:


--------------------





Indoor Greenhouse Build 
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Invisiblemushball
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Re: Pasty Agar Tek *DELETED* [Re: TravelAgency]
    #23678464 - 09/26/16 12:01 AM (7 years, 4 months ago)

Post deleted by mushball

Reason for deletion: .



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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: mushball]
    #23678474 - 09/26/16 12:07 AM (7 years, 4 months ago)

You can. And I'd start here and then go lookup how Frank gets shit done. He has a pretty good outline of bulk growing. Also read links in peoples signatures. Usually Lots of good info there


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleSupalemonhaze
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Re: Pasty Agar Tek [Re: mushball]
    #23678601 - 09/26/16 01:53 AM (7 years, 4 months ago)

Quote:

mushball said:
Thanks for all this great info. 
  After you make them do you just store them inside a sab until they are ready to transfer to another plate?  What other teks would you recommend to go with this for someone who has only ever done brf cakes?




Depends on how many plate you make.  If you use a handful of plates at a time, you can definitely throw them in the SAB but you can store them wherever you like if you need the SAB space.

As for a "tek", if you mean a tutorial on sterile tek, there isn't one on here. Best you can do is take a look at RR's videos (or any video made by a good cultivator really) and watch him work with agar. With sterile tek, seeing is better than reading. All that matters when using a SAB is that the air is as still as possible when you open your plates and that nothing goes over your open plate unless it has been sterilized (like your flame sterilized loop or scalpel). Never hover your hand over an open container.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Supalemonhaze]
    #23678687 - 09/26/16 03:51 AM (7 years, 4 months ago)

Not gonna link Munch's videos?  For shame.

http://www.shroomery.org/forums/showflat.php/Number/21032261


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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