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circles

Registered: 06/15/16
Posts: 344
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I've used up all my 40 pre-poured agar dishes I've ordered a while ago to get started without too much hassle but finally have put my PC to a good use making Pasty Plates.
Couldn't find the round Glad minis around here but came across these

Just whipped up 8 of them in no time.

In terms of handling it was not the most pleasant experience in the SAB because of the screw on lids but manageable.
I've started cultures from spores (subaeruginosa) for an out door patch to inoculate grains.
What I like about it in my case is the following:
The pre-poured agar dishes I've bought were probably high in nutrition which caused slow and fluffy / flimsy growth and the mycellium was pissing all over the place from the center on outwards, I first thought it was contam.
I have done the first transfer from one master plate to the last two of pre-poured agar dishes and 10 days later I whipped up the pasty plates and transferred from the same sectors of the same master plate which was 3 days ago. The difference in growth is quite noticeable I think.
pre-poured dish after 13 days

pasty plate after 3 days

Cool bananas!
Edited by circles (09/02/16 04:17 AM)
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circles

Registered: 06/15/16
Posts: 344
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Re: Pasty Agar Tek [Re: circles]
#23603652 - 09/02/16 03:54 AM (7 years, 4 months ago) |
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I got a couple of questions though.
I had quite heavy condensation in my plates......shook them and poured the excess water out. That got rid of most of the water but there are still droplets on the lid and sides which makes it a bit difficult on some plates to have a proper look at the point of interest.
Now do you guys think it might be a good idea to sterilize a bunch of cut up paper towels same time doing the pasty plates in the PC? When serilized one could wipe the lids clean with them. In my case the lids would provide good sight if not obscured by moisture. Well I'll try it any way, but it will be a while till then.
Second question:
As Pasty stated in OP "make sure the agar doesn't contain any sugar". I mean we are adding a drop of glucose syrup to the mix so if I would refrain from doing so, would it be ok if the agar I use would contain 0.2g of sugar per 5g of agar. This would basically count up to the same amount of carbohydrates, right?
used the non sugar containing agar for the plates but am curious as I got the sugar type as well.
Edited by circles (09/02/16 04:08 AM)
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Pasty Agar Tek [Re: circles]
#23603873 - 09/02/16 07:12 AM (7 years, 4 months ago) |
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first: just leave the small amount of condensation after you have shaken and gotten the most of it out in SAB. it will dry up in a few days/week.
second: i guess if it would come out to be about the same, then it should be fine. just dont use any extra sugar with it. im not sure if there needs to be a "certain type" of sugar for it, though. i think it does need a specific type, but i really cant remember right now. and maybe most sugars are this specific type, or at least break down to that type.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Check the other ingredients. It was something extra in the presweetened agar that was making it less ideal.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Grey]
#23604041 - 09/02/16 08:59 AM (7 years, 4 months ago) |
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It's because its refined sugar which makes cultures lazy.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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i knew it might have been specific sugar problems! i knew i had read you want and dont want certain types of sugars =p
thanks pasty.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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circles

Registered: 06/15/16
Posts: 344
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yeah thanks guys........much appreciated  
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
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Re: Pasty Agar Tek [Re: circles]
#23608953 - 09/03/16 06:43 PM (7 years, 4 months ago) |
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Quote:
circles said: I've used up all my 40 pre-poured agar dishes I've ordered a while ago to get started without too much hassle but finally have put my PC to a good use making Pasty Plates.
Couldn't find the round Glad minis around here but came across these

Just whipped up 8 of them in no time.

In terms of handling it was not the most pleasant experience in the SAB because of the screw on lids but manageable.
I've started cultures from spores (subaeruginosa) for an out door patch to inoculate grains.
What I like about it in my case is the following:
The pre-poured agar dishes I've bought were probably high in nutrition which caused slow and fluffy / flimsy growth and the mycellium was pissing all over the place from the center on outwards, I first thought it was contam.
I have done the first transfer from one master plate to the last two of pre-poured agar dishes and 10 days later I whipped up the pasty plates and transferred from the same sectors of the same master plate which was 3 days ago. The difference in growth is quite noticeable I think.
pre-poured dish after 13 days

pasty plate after 3 days

Cool bananas!
Where'd you find those, how much did they cost, and are they flat bottomed? The one draw back to the glad mini's is the indentation on the bottom. If there are readily available flat alternatives I'd be down for trying them out.
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Kenetic
Nam Sayin



Registered: 08/24/14
Posts: 4,389
Loc: I don't believe in land
Last seen: 5 years, 3 months
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I think I might try the pasty tek just to get a fruit to clone from. Those containers look like excellent little tiny infant baby microclimates.
-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Kenetic]
#23609079 - 09/03/16 07:30 PM (7 years, 4 months ago) |
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Pft, Microclimate? Nonsense.
     
Not all "pasty plates" but you get the idea. Sterile clones launched me from noob to boob pretty quick I found.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Quote:
Pastywhyte said: It's because its refined sugar which makes cultures lazy.
LOL .
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Terpfreak said:
Quote:
Pastywhyte said: It's because its refined sugar which makes cultures lazy.
LOL .
Alright I give up, what's so funny? The parallel between mycelium and humans where refined sugar also makes us act like lazy shits? Or something way simpler than that?
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Pasty Agar Tek [Re: Inocuole]
#23609206 - 09/03/16 08:02 PM (7 years, 4 months ago) |
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There was recently a series of test done on rats where refined sugar and cannabis were used to "prove" that cannabis was making them lazy. Im assuming thats what he was reffering to- if not- I just find humor where I want to I guess.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I think he was being serious and that just puts into perspective how stupid that cannabis study must've been. Like, two variables, but you're associating the results with only one of them? Unscientific as fuck.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Pasty Agar Tek [Re: Inocuole]
#23609215 - 09/03/16 08:04 PM (7 years, 4 months ago) |
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Well of course but it was funded and those are the only tests that get to people's ears.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: Terpfreak] 1
#23609219 - 09/03/16 08:06 PM (7 years, 4 months ago) |
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Well fuck people with ears.
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Terpfreak
❀Terpenes❀



Registered: 07/08/16
Posts: 1,065
Loc: Land Of Ooo
Last seen: 5 years, 10 months
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Re: Pasty Agar Tek [Re: Inocuole]
#23609224 - 09/03/16 08:06 PM (7 years, 4 months ago) |
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circles

Registered: 06/15/16
Posts: 344
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Quote:
TravelAgency said: Where'd you find those, how much did they cost, and are they flat bottomed? The one draw back to the glad mini's is the indentation on the bottom. If there are readily available flat alternatives I'd be down for trying them out.
I went browsing through all sorts of variety shops for usable containers and found these at a clearance sale. That was at a Sunny's Variety Store (NZ) and the brand name is "Snazzee". But these are made in China and I came across them in a couple of other stores having different lables on them. Payed about US$ 1.20 each (seen them for up to $3.00 elsewere)
Yes they are flat and got a clear window on the lid so its easy to see whats going on inside.
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TravelAgency
The ongoing "wow"


Registered: 12/25/10
Posts: 4,431
Last seen: 11 months, 22 days
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Re: Pasty Agar Tek [Re: circles]
#23609405 - 09/03/16 09:06 PM (7 years, 4 months ago) |
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Cool thanks for the info I will look them up
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xPTxHIPPIe
Inspired


Registered: 06/13/16
Posts: 273
Loc: Desert
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So first of all I am completely new to cultivation and have only done 2 MS brf cake grows.
Thanks to Pasty agar tek I think im on the right track. Got some experience to gain and some dialing in to do but I really owe my future progess to this tek.

Getting started id like to say all the instructions are really easy to follow agar is easy to make and prepping the containers was easy as well. I think the hardest part is getting the pressure right but im a complete noob.
 So after making these i noticed i had some condensation alot to be honest but i did some reading and i think i have nothing to worry about if everything fails its a learning experience and ill try again but off to the SAB for some spores.

Now theres nothing like seeing all my work on the shelf as it "hopefully grows" btw knocked up 12 jars pf style with the remaining multiapore solution. One thing I think I ran out of solution on that last jar but I did what I could.
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