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OfflineBaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
Re: Pasty Agar Tek [Re: Josex]
    #23492202 - 07/30/16 10:33 AM (7 years, 5 months ago)

I own a shit load of Tyvek and have RTV and all the drug store area of town is heavy construction traffic

The RTV comes out less of a hassle for me at this time


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<iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>


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OfflineCurious Shroomer
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Registered: 05/19/16
Posts: 118
Loc: Germany
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Re: Pasty Agar Tek [Re: BaronVonBud]
    #23524513 - 08/09/16 10:27 AM (7 years, 5 months ago)

9 Plates in the PC currently...gona do another 9 afterwards. Planing to put a drop of ATL#7 Spore Solution on it on Friday. Hoping to get a clean Plate to inoculate some Jars :mushroom2:

PS: Only 2 of the containers have a slight condensation...now waiting for 2-3 days if any contams show up and then gona start with the Spores.


Edited by Curious Shroomer (08/10/16 01:39 AM)


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OfflineBaronVonBud
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Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
Re: Pasty Agar Tek [Re: Curious Shroomer]
    #23528736 - 08/10/16 04:30 PM (7 years, 5 months ago)

My condensation cleared up in a few days


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<iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>


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OfflineCubensiscat
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Registered: 01/04/16
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Last seen: 7 years, 4 months
Re: Pasty Agar Tek [Re: BaronVonBud]
    #23528769 - 08/10/16 04:40 PM (7 years, 5 months ago)

I can't wait to give this tek a try.  I just received my supplies to get the job done. So stoked!!


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OfflineCurious Shroomer
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Registered: 05/19/16
Posts: 118
Loc: Germany
Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: Cubensiscat]
    #23535106 - 08/12/16 02:40 PM (7 years, 5 months ago)

Made 20 Plates...1 "broke" when i put the lid on...use too much force :P

Build an SAB and put some Spore Solution from a Spore-Vial on 4 of em. Opened 1 up and touched it just for the fun of it since i wanna see how the Stuff grows.

Hopefully i didnt fuck up and Myc will grow on the 4 Plates and god knows on that one :P


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Offlineesse_jeremy
the deconstructor
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Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: Curious Shroomer]
    #23536404 - 08/12/16 10:12 PM (7 years, 5 months ago)

Quote:

Curious Shroomer said:
Made 20 Plates...1 "broke" when i put the lid on...use too much force :P

Build an SAB and put some Spore Solution from a Spore-Vial on 4 of em. Opened 1 up and touched it just for the fun of it since i wanna see how the Stuff grows.

Hopefully i didnt fuck up and Myc will grow on the 4 Plates and god knows on that one :P




:whathesaid:


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things does not exist,
everything is a process, so we proceed


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InvisibleFractalXplora
Grainiack
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Registered: 02/11/06
Posts: 2,494
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Re: Pasty Agar Tek [Re: esse_jeremy]
    #23540156 - 08/14/16 08:48 AM (7 years, 5 months ago)

nice tek Patsy! I been suffering all my life pouring petris in a SAB.Yes I get  90% clean plates, but wht a nightmare it is fumbling around in there! No pour here I come! :cookiemonster:


Got some glass jars on the way to try this out.



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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
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Re: Pasty Agar Tek [Re: FractalXplora]
    #23540192 - 08/14/16 09:07 AM (7 years, 5 months ago)

Yep its pretty handy for the simple hobbiest :thumbup:

I should clarify that I didn't invent no pour. I just made it popular :grin:


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Re: Pasty Agar Tek [Re: FractalXplora]
    #23540223 - 08/14/16 09:23 AM (7 years, 5 months ago)

Quote:

FractalXplora said:
nice tek Patsy! I been suffering all my life pouring petris in a SAB.Yes I get  90% clean plates, but wht a nightmare it is fumbling around in there! No pour here I come! :cookiemonster:


Got some glass jars on the way to try this out.





Build a bigger SAB seems like an obvious answer too regardless of if you'll continue to pour or not. If it's not comfortable to pour plates in its built wrong


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Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Pasty Agar Tek [Re: bodhisatta]
    #23543042 - 08/15/16 06:37 AM (7 years, 5 months ago)

Any tips for taking photos of what's going on inside the tubs? :justdontknow:

All photos taken come out potato, even when using the right lens and camera for the shot. The plastic and condensation that remains on the walls seem to be throwing off the focus of the photo. Opening the tub and taking a photo seems to be the simplest and most obvious solution, would it be okay to open the lid, keep the tub sideways or upside down (to prevent anything from landing on the agar) and photograph the plate?


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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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Invisiblemupetmower
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Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Pasty Agar Tek [Re: h0ldthedoor]
    #23543105 - 08/15/16 07:24 AM (7 years, 5 months ago)

you would want to do it in a SAB.. i would maybe wait to take the pic until right before/after your making the transfer or something. but if youre careful about it, i guess it doesnt really matter.. i just take a pic with my phone, from the side of the plate once the condensation goes down a little(which it will, just give it time), and they tend to come out decent enough to get a good idea of whats going on.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleh0ldthedoor
HODOR
I'm a teapot User Gallery


Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Pasty Agar Tek [Re: mupetmower]
    #23543569 - 08/15/16 10:44 AM (7 years, 5 months ago)

Quote:

mupetmower said:
you would want to do it in a SAB.. i would maybe wait to take the pic until right before/after your making the transfer or something. but if youre careful about it, i guess it doesnt really matter.. i just take a pic with my phone, from the side of the plate once the condensation goes down a little(which it will, just give it time), and they tend to come out decent enough to get a good idea of whats going on.




Right, anytime the plates are open it will be in a SAB, probably should have mentioned that. It would be nice to wait until just before making the transfer, but that's the whole purpose of trying to get a photo for feedback; to know if the plate is ready to have something done with it. Yeah the camera phone was close to worthless, it would begin to focus on the growth but then lose it's focus while trying to focus on the plastic wall of the tub or the condensation on the inside of the tub (despite the plates having already set for ~14 days). So, a macro lens and proper camera were used to photograph the plate and the results were still potato, as you can see below; the camera phone photos were so bad they were just deleted. Tonight the plate will be opened on it's side and photos taken through one of the arm holes in the SAB.



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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
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Re: Pasty Agar Tek [Re: h0ldthedoor]
    #23543742 - 08/15/16 11:35 AM (7 years, 5 months ago)

I don't need the plate opened to tell you that the transfer looks well made but the culture looks sketch. Was that from spores or tissue?


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Invisibleh0ldthedoor
HODOR
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Pasty Agar Tek [Re: Pastywhyte]
    #23543753 - 08/15/16 11:38 AM (7 years, 5 months ago)

Spore swab (spore print from a wild fruit)


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Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: h0ldthedoor]
    #23543776 - 08/15/16 11:47 AM (7 years, 5 months ago)

Wild prints are often filthy. Based on that I'm just gonna suggest that is pure mold. If it's not, then the culture is either meshed with mold or super weak. I wouldn't mess with that one anymore.

If I was trying to work with a wild print I would either streak a dozen plates, use tetra or gentamiacin, a warm pour, sandwich the spores, or all of the above. Then chose the best colony to work with and throw the rest away.

Even tomentose mycellium can look strong or weak. You want robust growth no matter the form it takes.



These plates are both pretty tomentose but the one on the left is better. See the difference? On yours the center of the colony should thicken up as it gets consolidated but instead it's thin and weak compared to the leading edge. That's a real sign things are not right.


The good news is you have no visible satellites which at least means your technique is not at fault. You did the hard part right. It's not a loss if you learn something.


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Invisibleh0ldthedoor
HODOR
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Registered: 06/25/16
Posts: 510
Loc: North of The Wall
Re: Pasty Agar Tek [Re: Pastywhyte]
    #23544084 - 08/15/16 01:22 PM (7 years, 5 months ago)

Quote:

Pastywhyte said:
Wild prints are often filthy. Based on that I'm just gonna suggest that is pure mold. If it's not, then the culture is either meshed with mold or super weak. I wouldn't mess with that one anymore.

If I was trying to work with a wild print I would either streak a dozen plates, use tetra or gentamiacin, a warm pour, sandwich the spores, or all of the above. Then chose the best colony to work with and throw the rest away.

Even tomentose mycellium can look strong or weak. You want robust growth no matter the form it takes.



These plates are both pretty tomentose but the one on the left is better. See the difference? On yours the center of the colony should thicken up as it gets consolidated but instead it's thin and weak compared to the leading edge. That's a real sign things are not right.


The good news is you have no visible satellites which at least means your technique is not at fault. You did the hard part right. It's not a loss if you learn something.




Got it, the plate will be emptied for re-use.

Tetra, is this what you're referring to?

If so, how to use it? Found this post, but still not sure exactly how to use it. Is tetra added to the pasty plates agar recipe? If so, how much should be added?

Warm pour would/will be used if contams are still an issue on the antibiotic plates, right?

agar sandwich isolation tek is off the table since gentamicin is available by RX-only :frown:

I see the difference in those two plates, as you described. The plate on the right looks more like the plate at home, there's a little growth in the center and some visible agar between the center and the edge, while most of the action is taking place on the edge.

Thank you for the kind remarks about technique :smile:


--------------------
Always keep your foes confused. If they are never certain who you are or what you want, they cannot know what you are like to do next. Sometimes the best way to baffle them is to make moves that have no purpose, or even seem to work against you.

– Petyr Baelish


Edited by h0ldthedoor (08/15/16 02:04 PM)


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Pasty Agar Tek [Re: h0ldthedoor]
    #23545913 - 08/16/16 02:31 AM (7 years, 5 months ago)

You don't need antibiotic agar for sandwiching. I don't use antibiotic agar at all, I think of it as counter productive in the sense that if some bacteria is present, it will be dormant and much easier to miss.

The sandwich works by providing a solid barrier which the bacteria is not able to grow through since it's liquidy. However mycelium is able to grow through it, no problem. Once the mycelium breaks the surface of the agar sandwich, just scrape some healthy mycelium off and transfer that to a new plate. A sandwich done correctly can clean up the most bacteria ridden cultures in just 1 transfer.

Some sandwiches I have going atm.



1,2 and 3 are pretty much ready for a transfer but 4 and 5 still need another day. You can't see it in the photo but the mycelium already broke the surface just a little bit in the last two. In the last two, you can see just how much bacteria is in the plates but none of that is able to make it on top of the sandwich.


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OfflineCurious Shroomer
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Registered: 05/19/16
Posts: 118
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Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: Supalemonhaze]
    #23551617 - 08/17/16 10:42 PM (7 years, 5 months ago)

Cleaned up five Plates yesterday since they got contaminated....OK one of em was the one i touched to see whats is going to grow.

Out of the 4 i dropped some Spore Sollution on two went bacterial without much room left for Myc and two other Plates got contaminated som other way.

Dropped a Rye Berry from my colonized Jars on a two additional Plates on Saturday since i had a feeling that the Spore Sollution to agar wont work out all to good.

The two Grains became really fluffy and it looks like Myc finally went onto the Agar over night. Gona Post a Picture later since im not sure if it is OK that the Grain got so fluffy.


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OfflineCurious Shroomer
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Posts: 118
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Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: Curious Shroomer]
    #23552595 - 08/18/16 08:29 AM (7 years, 5 months ago)

Here are the Photos...they are not really great but i hope you can see the fluffyness :crazy2:

The other Plates that i innoced with the Spore Sollution starting to look kinda bacterial too...guess i wont try using a Spore Vial for agar again.



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Offlinegoodnightmoon
Stranger
Registered: 07/29/16
Posts: 5
Last seen: 7 years, 5 months
Re: Pasty Agar Tek [Re: Curious Shroomer]
    #23553389 - 08/18/16 01:56 PM (7 years, 5 months ago)

Hi guys, I'm totally noob with agar but interested. Few questions:

-Do steaming them for like 90minutes work or they need to be pressure cooked? I know that's better, and steam can only work with brf jars, but thats possible? Heard someone doing it in microwave. ?

-Where I live we don't have potato flakes. What is the meaning for adding potato flakes? Agar by itself not work? What if someone alse add something else, like brown rice flour (sorry for it is stupid question, but that is nutrious).

-With agar work, you def. need some (surgical) scapel or something, no? (to make transfers). I'm curious about cleaning a spore print.
Agar is VERY prone to contam, am I right?

I know there are easy questions, but this tek is really interesting, making me understand some of the basics.

Thanks,

Greets,


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