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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Thanks. And yeah I know what the causes are. By like Pasty added how to check each one seperate That's what I needed to figure out its all over the plates I just did a third set with some smaller Round containers to try that Just cus it's easier for me to hold the lid off just a crack and just get a small drop in center I had the remainder of my Agar mix I poured j to a mini round and it's been jn for 2days now and I will check when I get home. It only took 2days for this stuff to show up. So I'll see if it has anything when I get home. It's just to check and see if sterilization is working properly or if it's not enough time.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Skillzd]
#23441003 - 07/14/16 02:09 PM (7 years, 6 months ago) |
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You're still gonna have to open it for transfers and grain inoculations. Best to get used to working in a SAB and get it right.
You could use a syringe to inoculate throught the hole but again, you will still have to open it at some time. You would still have to use a SAB if you are going to use the GE hole as the syringe is going to tear the micropore tape so you need to work with sterility anyway. The agar will not be as exposed as opening the container though. Just use another piece of tape to cover the one you tore with the syringe if you choose to go down that route.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Yeah that's what I was thinking as well. And I know I'd have to open it but atleast maybe for now I could get some thing going and it will help me tell where or what it is coming for sure. Plus atleast if I can get some Myc growing and do the transfers to minimize and eliminate Contams Um. I know I have mentioned this before. But my area in working in. I'm in an RV at the kitchen booth. I turn everything up front off and just the A/C in the back bedroom has to be on or its instantly 90deg in here. No doors. Bathroom area is open Cat box is open to the area Laundry. My plants And then kitchen. I clean everything good the day before and then wipe down and spray again. But even with the SAB I think air is still moving too much. I know you guys could do it but I'm newbie lol. My sterile technique needs work. But honestly I'm doing everything as careful as Ali can and should not see this much contamination So. With that reminder or my area. If I made a SAB / GB combination with filtered air inlet for the suction so no dirty air can come in at all. Would this be a better route for me FOR NOW atleast. I can work on my sterile techniques as I go and improve on them. But I want to atleast get something started
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Pasty Agar Tek [Re: Skillzd]
#23441266 - 07/14/16 04:10 PM (7 years, 6 months ago) |
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Quote:
Skillzd said: If I made a SAB / GB combination with filtered air inlet for the suction so no dirty air can come in at all. Would this be a better route for me FOR NOW atleast. I can work on my sterile techniques as I go and improve on them. But I want to atleast get something started
absolutely not. SAB 100%. make sure you spray the SAB down with some water before you goto work. contams in the air will get trapped on the wet walls instead of bouncing around.
Chill on the spaces, too. It makes it difficult to read your posts.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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What he said, don't do the glovebox thing. I used to spray the box all around with a 10% bleach solution, I guess that's kind of anal, but worked great. Let the box settle too, for at least 15 minutes before going back in there to work after prepping it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Water is fine.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Re: Pasty Agar Tek [Re: Inocuole]
#23441958 - 07/14/16 08:22 PM (7 years, 6 months ago) |
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I sprayed t down with 50% Iso. Figured that should do it for sure. Lol. Honestly I think it's in the agar mix and not sterilizing good enough. Need a good PC. For now I am trying to steam it in there again for a ridiculously long amount of time. I'm also trying some smaller vials I have that may be easier for me to sterilize and keep Contams out and away easier. Thot it was worth a try u til I get a PC. I'm still trying to narrow it down yet the right way. Just wanted to try smaller Vials in the mean time while I'm waiting. just to be sure but most likely it's not sterile enough cus broken PC I've been trying to use for it. I guess it's time. Too bad rents due Saturday. Hopefully I can still get it tho Monday when my partner gets paid. I'll be back when these show me something tho. Thanks everyone
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Pasty Agar Tek [Re: Skillzd]
#23441972 - 07/14/16 08:27 PM (7 years, 6 months ago) |
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Quote:
Skillzd said: I sprayed t down with 50% Iso. Figured that should do it for sure. Lol. Honestly I think it's in the agar mix and not sterilizing good enough. Need a good PC. For now I am trying to steam it in there again for a ridiculously long amount of time. I'm also trying some smaller vials I have that may be easier for me to sterilize and keep Contams out and away easier. Thot it was worth a try u til I get a PC. I'm still trying to narrow it down yet the right way. Just wanted to try smaller Vials in the mean time while I'm waiting. just to be sure but most likely it's not sterile enough cus broken PC I've been trying to use for it. I guess it's time. Too bad rents due Saturday. Hopefully I can still get it tho Monday when my partner gets paid. I'll be back when these show me something tho. Thanks everyone
ABSOLUTELY DO NOT SPRAY DOWN YOUR SAB WITH ALCOHOL
#1, the vapors are extremely flammable. we had a recent user this year goto the ER after spraying his SAB with ISO then proceeded to work in front of it. It exploded out of the arm holes and into his face.
#2, alcohol evaporates much faster than water. you want the walls wet, not dry. alcohol will dry out much faster.
#3, its a still air box, not a sanitary air box. tap water is 100% acceptable to use.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 21 days
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Lmao. I had a little scare today when my gloved hands were still a bit wet with iso and I lit my torch up
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Highwalker
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Re: Pasty Agar Tek [Re: Skillzd]
#23442112 - 07/14/16 09:18 PM (7 years, 6 months ago) |
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I may be new to mushroom. But I'm not completely new. Lmao. One I don't light anything inside. And 50% is hard to keep lit when burning it straight open alchol. Let alone some fumes coming outside the SAB into the air. I mean maybe if you douche it with straight 50% the fumes may. I mix mine with water. Sorry I didn't state that. I've been dealing with flammables on and off my whole life and never have had a bad incident that caused injury. Yet! know the limits. Lol And alcohol dries up nicely once evaporated and no fumes. I have it mixed with water anyways but am goin to just use soapy water from now on since everyone says ya don't need anything beside water.
It's like so many people have lost money to me cus I bet them $100 that I can throw a cogerrette in a bucket of gas and it won't ignite. Lmao. I've won some money doing that It doesn't ignite unless the cig hits the ground and the cherry sparks the gas on the ground enough to ignite. But a bucket of gas won't. You'd be surprised how many will argue that it will tho. Lol.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Re: Pasty Agar Tek [Re: Skillzd]
#23442127 - 07/14/16 09:21 PM (7 years, 6 months ago) |
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But I do get the wet walls. That I can see helping a lot. Anytime I use alcohol when I'm using flame. It's diluted enough and the fumes settle and is mostly evaporated before j even start working. I was in a house fire. I don't like burns. I'm careful with that stuff and know the limits. As soon as I open my ISO it gets watered down so I couldnt grab the wrong bottle even
Edit. I'm glad you do tell people tho. The one person you don't will hurt themselves. So good looking out
Edited by Skillzd (07/14/16 09:27 PM)
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Pasty Agar Tek [Re: Skillzd]
#23442130 - 07/14/16 09:21 PM (7 years, 6 months ago) |
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that's not the same. If you drown a cogerrette's cherry, no matter the liquid, it'll be doused. But we're talking about torches here.
Also, you completely ignored what Munch said about the walls drying; we don't want that. You want walls wet with mist the whole time you're working in the SAB.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Yeah. I no that. You know that. But like I said. You'd be suprised at how many don't. Same with alcohol. It's good people say it cus many don't realize the fumes can. But I'm not buying anymore cus everyone says it's pointless
and yes i addressed that I underatood the walls being wet and will use water instead and soapy water for bottom area and towel. And thank you Munch. j new the towel and stuff to be moist and I figured it was moist enough with the moisture but j will make sure water only now and keep walls wet
Between the PC and that. I think it will improve much more. But I will try this before I even get the PC. Just cus I'm curious to see if it solves it first. I'm getting PC no matter what next week. I'm done fighting with this broken shit
Edited by Skillzd (07/14/16 09:27 PM)
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Re: Pasty Agar Tek [Re: Skillzd]
#23442175 - 07/14/16 09:32 PM (7 years, 6 months ago) |
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Oh. And the last thing you said about the SAB not being sanitary. Yeah I've been a bit OCD about keeping it as sterile as possible atleast but I think at first j was more worrying about being sanitary than Still Air. I assumed it's in a closed box so it's still. Now it has to be sterile too. But as I've been told. I stopped worrying so much about that and improved it so it was still air. And making an outer area since my RV has air moving around. I need a half. Decent place to use my SAB. cus I'm thinking correct. If the air is moving in the room. It won't do its job. And although I shut almost everything off. The AC is still on in the back cus of how hot it is. I'm going to do it at night when it's cool now too and I can shut it all off and no air moving whatsoever
Edited by Skillzd (07/15/16 11:26 AM)
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: Pasty Agar Tek [Re: Skillzd]
#23483075 - 07/27/16 05:30 PM (7 years, 6 months ago) |
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I searched around about this but couldn't find the answer. I've been out of the loop for quite a while.
Going from MS syringe to PDA plates I should mix 4oz water, drop of honey, 2.5g agar, 3.5g (about 2 teaspoons)potato flakes. But, what about for transfers? I am pretty sure you are supposed to lessen the nutrients in your recipe, is this correct? So then, how much of what would be ideal for making plates for transfers?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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It's impossible to say what is ideal given that every set of genetics will have their own preference. They are also more than capable of growing on a range of media.
I only change stuff like stiffness or nutes if I need to. Stubborn spores? Softer agar. Tomentose growth? Less nutes. Often enough most cultures or spores won't give a shit if stuff changes anyways.
The range I work in is between 1.5-2.5% agar and 1-4% nutes.
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Thanks pastywhyte!
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BaronVonBud
Stranger

Registered: 07/14/16
Posts: 143
Last seen: 7 years, 3 months
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Do you see any issues or tweaks to make if using Tyvek instead of micro-pore tape ? It would be sealed on with RTV completely around the GE hole
-------------------- <iframe src="//giphy.com/embed/Z7TvUbQHuFdBu" width="480" height="352" frameBorder="0" class="giphy-embed" allowFullScreen></iframe><p><a href="http://giphy.com/gifs/rock-flag-eagle-Z7TvUbQHuFdBu">via GIPHY</a></p>
Edited by BaronVonBud (07/29/16 01:26 PM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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And why don't you want to use MP tape as per the tek? It works man, no need to fuck around with RTV. Add two layers of MP tape if that gives you more peace of mind.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Josex]
#23491366 - 07/30/16 05:05 AM (7 years, 5 months ago) |
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It would work fine, it's a hassle to do though compared to sticking a couple of layers of tape on your lids. However, if micropore is unavailable, tyvek would do well in it's stead.
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