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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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Re: Pasty Agar Tek [Re: Skillzd]
#23435973 - 07/12/16 08:06 PM (7 years, 6 months ago) |
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Yep b that's the same one
That's what made me want to buy it. That damn article right there about the thing. Plus the guy said it does work really good
Edited by Skillzd (07/12/16 08:08 PM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pasty Agar Tek [Re: Skillzd]
#23436684 - 07/13/16 12:43 AM (7 years, 6 months ago) |
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At least it makes the grains extra tasty for all the contams left in there 
I think that it works simply by sealing the oven really well. the heat would build a small amount of pressure well enough on it's own I think.
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guyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
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Quote:
spacechildo said: did a quick search for pressure ovens, found only 1 manufacturer and some infomercials, and this is under "how it works"
"The new Wolfgang Puck Pressure Oven is equipped with Flavor Infusion Technology™ - a breakthrough in cooking which features a moisture- sealed, low-pressurized chamber that infuses heat, flavor, and moisture into your food. Nothing escapes – creating the most flavorful and moist meals you have ever had!"
Flavor infusion techTM 
am i the only one that reads this in Wolfgang Puck's voice in their head when reading this?
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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. Yeah. Contams have some tasty grains for sure. lol I can't stop thinkin about, a nice beef roast on the rotisserie it comes with. I'd be cooking everything in that anyways but for what we do here I don't think it will work. The person even gave me a better deal. Technically free. I just have to drive 45min with my work van needing a repair and detail her mothers car for the Oven Awesome. But it's just too far for my van right now And I found a bran new one for $110. So I'll get one eventually for the RV to cook. And then maybe play with it
Edited by Skillzd (07/13/16 09:48 AM)
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GreenRabbit
Plutonium Pollinator



Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
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Re: Pasty Agar Tek [Re: guyute22]
#23437505 - 07/13/16 10:07 AM (7 years, 6 months ago) |
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Quote:
guyute22 said:
Quote:
spacechildo said: did a quick search for pressure ovens, found only 1 manufacturer and some infomercials, and this is under "how it works"
"The new Wolfgang Puck Pressure Oven is equipped with Flavor Infusion Technology™ - a breakthrough in cooking which features a moisture- sealed, low-pressurized chamber that infuses heat, flavor, and moisture into your food. Nothing escapes – creating the most flavorful and moist meals you have ever had!"
Flavor infusion techTM 
am i the only one that reads this in Wolfgang Puck's voice in their head when reading this?
I did. I kind of want one now. For cooking though. Certainly won't work for mycology.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Hey so I used those two plates for blenderless lc, I have a 3rs plate I thought was coming along nicely but I spotted a contam today, could I still inoculate with a piece of this plate or should I transfer and wait it out?
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Quote:
Pastywhyte said: Here is what I'm talking about. . .

is that an electric pc?? it works also if is not stove???
wow an electric pc will change my life as I don't need to go in the kitchen to do my pc cicles!!!!
wich model is that and wich model do you suggest me to buy??
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things does not exist, everything is a process, so we proceed
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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You got a g to drop?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
esse_jeremy said:
Quote:
Pastywhyte said: Here is what I'm talking about. . .

is that an electric pc?? it works also if is not stove???
wow an electric pc will change my life as I don't need to go in the kitchen to do my pc cicles!!!!
wich model is that and wich model do you suggest me to buy??
Its an electric sterilizer. AA 75X.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Quote:
Pastywhyte said:
Quote:
esse_jeremy said:
Quote:
Pastywhyte said: Here is what I'm talking about. . .

is that an electric pc?? it works also if is not stove???
wow an electric pc will change my life as I don't need to go in the kitchen to do my pc cicles!!!!
wich model is that and wich model do you suggest me to buy??
Its an electric sterilizer. AA 75X.
Oh my lawd.. That would be so convenient... haha I NEED one!! One day..
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Highwalker
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Quote:
Pastywhyte said:
Quote:
esse_jeremy said:
Quote:
Pastywhyte said: Here is what I'm talking about. . .

is that an electric pc?? it works also if is not stove???
wow an electric pc will change my life as I don't need to go in the kitchen to do my pc cicles!!!!
wich model is that and wich model do you suggest me to buy??
Its an electric sterilizer. AA 75X.
ahh ok, I assume that the electric sterelizer used for the babies bottle don't work for sterelize agar and stuff right???
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things does not exist, everything is a process, so we proceed
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ErikXL45
SoME AssHoLe

Registered: 04/14/16
Posts: 142
Last seen: 3 years, 2 months
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Okay so followed the tek as closely as I could. I still have too much condensation on the walls of the mini rounds. I have them in a un-modified tote on top of a heating pad set to 75°F- on top of egg crates with a layer of newspaper right now. Lid on. Does this sound like the fastest route to getting rid of the condensation problem at the moment? This is my main question I need answered right now. I'm still working on timing raising and lowering the pressure when pc'ing them. They aren't boiling up on the sides so the condensation is happening at a different stage. I'm aware the answer may be in this thread...of the 236 current pages. But I was hoping for a shorter answer. Thanks for any help.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Re: Pasty Agar Tek [Re: ErikXL45]
#23438227 - 07/13/16 04:19 PM (7 years, 6 months ago) |
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Quote:
ErikXL45 said: Okay so followed the tek as closely as I could. I still have too much condensation on the walls of the mini rounds. I have them in a un-modified tote on top of a heating pad set to 75°F- on top of egg crates with a layer of newspaper right now. Lid on. Does this sound like the fastest route to getting rid of the condensation problem at the moment? This is my main question I need answered right now. I'm still working on timing raising and lowering the pressure when pc'ing them. They aren't boiling up on the sides so the condensation is happening at a different stage. I'm aware the answer may be in this thread...of the 236 current pages. But I was hoping for a shorter answer. Thanks for any help.
to avoid condensation try to heat up your PC slower (very slow) and let it cool down slower before open it, also try to use 2 layers of foil to wrap your plates
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things does not exist, everything is a process, so we proceed
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 24 days
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Re: Pasty Agar Tek [Re: Inocuole]
#23438262 - 07/13/16 04:28 PM (7 years, 6 months ago) |
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Quote:
Josex said:But I just don't get why you even bother with microwaving agar. Why don't you just pc a whole bunch of plates? It seems easier and quicker if you need to sterilize a number of plates and it is a reliable method 
It suits me and requires far less tending to than my PC, some peoples situations are different. I find it very reliable.
Quote:
Inocuole said: PCing is not a quick process..
Exactly. I often prepare agar just before leaving the house. Microwave for ~2mins until boiling, then set to 20-30mins on defrost and leave, come back hours later and its ready to use.
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ErikXL45
SoME AssHoLe

Registered: 04/14/16
Posts: 142
Last seen: 3 years, 2 months
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Quote:
esse_jeremy said:
Quote:
ErikXL45 said: Okay so followed the tek as closely as I could. I still have too much condensation on the walls of the mini rounds. I have them in a un-modified tote on top of a heating pad set to 75°F- on top of egg crates with a layer of newspaper right now. Lid on. Does this sound like the fastest route to getting rid of the condensation problem at the moment? This is my main question I need answered right now. I'm still working on timing raising and lowering the pressure when pc'ing them. They aren't boiling up on the sides so the condensation is happening at a different stage. I'm aware the answer may be in this thread...of the 236 current pages. But I was hoping for a shorter answer. Thanks for any help.
to avoid condensation try to heat up your PC slower (very slow) and let it cool down slower before open it, also try to use 2 layers of foil to wrap your plates
I didn't turn the heat down in increments really. But it cooled for around 6hrs before I opened up the pc. I do remember the paper towels being a little damp but not wet or saturated. I understand my technique will help avoiding this problem more in the future but my current question still stands- Quickest way to get rid of the condensation? My biggest issue is visibility and possibility of flooding the culture if I don't get rid of it.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: ErikXL45]
#23438481 - 07/13/16 05:35 PM (7 years, 6 months ago) |
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It usually dissappears in a couple days on its own. Not something to get really worked up over. If it is still there when you go to inoculate you can just dump it out on the bottom of the SAB when you go to inoculate it.
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Skillzd
Stranger
Registered: 06/11/16
Posts: 197
Last seen: 7 years, 3 months
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I'm still having a bit of trouble pasty. I've read a few people say they had trouble with the contam on agar and tried using there SAB in different areas and no luck until they found a nice spot to work in. I however don't have that option and the booth in the RV is where I work with the SAB. I've tried a few things now and still getting full of bacteria. Not as bad as last time tho. It better. Is there a way to Inocculate the agar with MS without opening the lid. I couldn't really use a loop then so I would j go about trying that? I'm thinking if I can avoid opening them all together then maybe then I can get something good
Btw. I'm waiting these plates out. I want to see what happens cus its almost like a crystallization on top this time. We have very very alkaline water too. Could that cause some kind of crystallization when it dries or no? In a pic it just looks like white bacteria starting. But in person I can see it's like crystals. Mini crystals Kind of like the stuff they use here to treat the water. I mean this water is like a 8.0ph and higher. I can't use it for my plants. But I didn't think about it when mixing the agar until I seen the crystal stuff on top of it now again. Or am I just plucking threads there and hoping it is. Lol. I will try my rain water I filter for plants to see but wanted to ask too
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Pasty Agar Tek [Re: Skillzd] 1
#23439228 - 07/13/16 09:41 PM (7 years, 6 months ago) |
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Well first bacteria is rarely airborne. If it was the workspace it would be mold colonies. The 3 usual causes of bacteria is insufficient sterilization, the inoculate, or the cultivator.
So to test the first you leave a plate unopened and uninoculated. If it goes bacterial you need to increase sterilization times.
For the second you try a different source of inoculate. New print, different syringe, etc.
Finally to test your procedure you do a dry run. Open plates, position the tools, everything except actually touch the spores to the plate.
If you are having issues with the syringe and liquid rolling around either sterilize a swab or a loop (see paperclip) put a few drops from the syringe on them and then put those to the agar.
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guyute22
Ugly Pig


Registered: 07/27/15
Posts: 504
Last seen: 5 years, 6 months
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I put SHIPs on my very first pasty plates. Only used once, and it worked, but I am not the biggest SHIP fan now, although it may be worth trying?
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Pasty Agar Tek [Re: guyute22]
#23440353 - 07/14/16 09:36 AM (7 years, 6 months ago) |
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that would be way more trouble that it's worth. it's really not that hard.
for syringe, flame needle outside SAB, squirt a drop out of needle to clear the barrel of the flamed liquid, open plate lid, squirt one small drop in center, close lid, done.
for loop, flame loop outside SAB, open plate lid, rub the loop on the surface of agar, rub loop onto part of the print to get spores on loop, rub loop back onto plate, close lid.
or for loop and syringe, flame loop and needle outside SAB, squirt a drop onto loop to cool it and clear barrel, squit one more drop onto loop for spores, open plate lid, rub across plate, close plate.
everything except for flaming should be done inside the SAB.
like Pasty said, it's probably going to be one of those three things that is causing contam.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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