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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429026 - 07/10/16 11:12 AM (7 years, 6 months ago)

Oh.  And I do see a Contam on the one I think.  It's a tan spot of stuff instead of the white like the rest.  Only in one I seen that tho


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429048 - 07/10/16 11:19 AM (7 years, 6 months ago)

Oh.  And the microwave article I linked    Like I said it was just interesting.    We also have to remember to that if some scientist goes and tests a microwave.  It's probaly a damn good expensive microwave and ours wouldn't compare shit to it.  Lol.  Maw but like k seen someone mention they can't even cook a burrito evenly. Let alone this stuff.  But I'd imagine some microwaves may work ok.  As others wouldn't at all.      Like mine for example.  Scared me when I first got it.  Put a cold cold cup of coffee in it and 20secs later it was boiling over and made a mess.  I was like WTF.  I Thot something was wrong  But it's just really powerful.    Where as the one in our old house a cup of coffee would take a full minute to get hot.  And it was bran new microwave.   
The biggest reason why it's not a good method I think is cus there are too many variables.


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429058 - 07/10/16 11:23 AM (7 years, 6 months ago)

those are toast, dude. sorry.

when you inoc your plates with spore syringe, just do 1 drop, as small as possible. or even better, flame a loop(paper clip or something) and put a drop of spore solution on it to cool it, then one more, then rub it over your plate.

also, are you sure you used the right amounts of agar, water, etc? it just looks... way off. and did you PC the plates?


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: mupetmower]
    #23429207 - 07/10/16 12:32 PM (7 years, 6 months ago)

Yep yep and yep.    It looks weird cus I scratched the surface of that one plate way too much.    Why are they TOAST tho?    I understand moisture.  But why then?    And I only used 2drops on them.  One in the pic I accidently used more.  It was the first one lilwhen I pushed a bit much and it Let out too much.    The other 3 got better as I went.  The last one being the best.   

The plates are Pastys TEK and I followed it to the T  The Agar didn't dissolve every last peice.  But was fine after PC.  Only one was a bit off.    And the moisture was high.  But I measured everything or exact    I may have put too much food coloring.  I didn't realize how easy they were to squeeze. But I doubt that extra few drops would hurt. 

My Agar is actual dried Agar too.  I had to chop jt and grind it into flakes and then start the TEK


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429213 - 07/10/16 12:35 PM (7 years, 6 months ago)

The one plate in the pic.  You can see a number 8 ?  Kind of.  That's why j Thot it looked weird.  I was going to number my plates but then realized I can't doe it that way.  I was going to do 8 but only did 4.      So that's the ONLY one I did that to

Making more tonight and will try to keep moisture drops down.

What do you think it is then?    Or cus I'm scratching the top of plate.  Don't do that at all?  Just rub it onto it smooth?    (With exception of the one I did the 8 in too much)


Edited by Skillzd (07/10/16 12:37 PM)


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429240 - 07/10/16 12:48 PM (7 years, 6 months ago)

Those plates are loaded with bacteria. Try to only use a drop and keep it from rolling around. There are a few ways to do this. As mentioned you can use a loop or swab with solution on it. Or you can melt a divot or trench with a hit scalpel or needle and the drop the spore solution in it to keep it in place.

But there is so much bacteria there it's hard to see if anything good is gonna be had. I am going to suggest the syringe is super bacterial given how bad it is.


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23429330 - 07/10/16 01:19 PM (7 years, 6 months ago)

Wow.    Are syringes suppose to come THAT loaded.      I Thot it was just starting weird cus of all the moisture but I guess not then.    M starting the Agar mix now again.    I have dried Agar I have the make into flakes.  I can use real agar that's dried.  Correct?    It's all seaweed.  Just some is dried. Some flakes. Some powder. Domes been melted into Bars already.  But I figured the closest to nature    Plus that's all they had here.  Out of 6 stores.  And some Powder Agar but once j got home k seem it says ingredients: agar, sugar.    So I took your advice in TEK and didn't use that stuff with added sugars.      Any tips on keeping the moisture in the containers down?      My PC doesn't build pressure to 15.  So it's like 5-8psi and steam.    It's broken so it's all I have right now.


Edited by Skillzd (07/10/16 01:20 PM)


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429342 - 07/10/16 01:25 PM (7 years, 6 months ago)

then, first of all, you need to PC for way longer, if it dose not hit 15psi. or even go to steam sterilizing, if the PC is that messed up.

Agar powder, or bars of agar is fine. just use the same amount that is called for. Im  guessing your plates are just loaded with bacteria because of the syringe, or the PC no doing ti's job. having a little excess moisture/condensation isnt all that big a deal if it isnt a LOT. i have excess all the time, but my plates still work fine.

Are you doing all of this in SAB, also? you need to, if not.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: mupetmower]
    #23429374 - 07/10/16 01:39 PM (7 years, 6 months ago)

i did.  It was in the PC for almost 3hours.  I started prepping at 4-430 and finally had j. In containers at 700-730 and took about an hour to cool nice in the SAB and then when they were cool for sure I nocced them.      Maybe I wait longer and try to get moisture gone complete BEFORE I Noc again.  I'll see how much Moisture I have those time.      I'm starting at 400.  I'll post a time when I start PC hot so j know how long for sure they are in this time.  I lost track last time and forgot they were in.  That's why so long too.


Edited by Skillzd (07/10/16 01:43 PM)


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429381 - 07/10/16 01:42 PM (7 years, 6 months ago)

And ok.  If you think moisture isn't an issue then I won't worry excessively about that.    I was just thknging too that the more moist the Container is with droplets.  The more Airborn Contams can stick.  But I'm in a SAB so should have much of any.    So it almost has to be syringe since its THAT bad.


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Offlinespacechildo
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Re: Pasty Agar Tek [Re: Pastywhyte] * 1
    #23429412 - 07/10/16 01:56 PM (7 years, 6 months ago)

if those pics are taken 3 days after inoc its safe to say you're using WAY too much inoculant.
try to squirt a drop on your loop and streak the loop across the plate.


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InvisiblebodhisattaMDiscordReddit
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Re: Pasty Agar Tek [Re: spacechildo]
    #23429419 - 07/10/16 02:00 PM (7 years, 6 months ago)

last link in my signature has some more agar information too

Quote:

Trusted Cultivator said:
Quote:

bodhisatta said:


take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish

if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that








Quote:

bodhisatta said:
Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.



Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.









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OfflineSkillzd
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Re: Pasty Agar Tek [Re: bodhisatta]
    #23429455 - 07/10/16 02:14 PM (7 years, 6 months ago)

Will do guys.  I have the Agar on now.  I made double so if I can remember this without looking I think I got it.  Lol.  5 grams of Agar.  4teaspons Potatoe Flake. 1 cup water.  And a drop of Karo


Can I ise a drop of Molasses?    That's what I have.


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429788 - 07/10/16 04:30 PM (7 years, 6 months ago)

Ok so it's been in PC and hot steamy for about 30 min now.  I'm going to let it in there until 7 atleast.    I started t at 530 and my burner is slow so it was hot and steaming at about 545pm      I figured with the slight pressure I have plus steam.  90min should be plenty of time  I'm thinking correctly on this right?      Atleast double the time of PC if not triple should do it I figured.  So min 90mon  Max 120min


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: Skillzd]
    #23429789 - 07/10/16 04:31 PM (7 years, 6 months ago)

A PC that works would go such a long way...


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Inocuole]
    #23430110 - 07/10/16 06:58 PM (7 years, 6 months ago)

Yeah I know.  And save me 2hours of extra time too.    I'm getting one soon.  But so far.  took out the two plates i had in there steaming for 2hours.  I took them out at 740 so that's almost a full two hours of steaming at 5-8psi.  The only reason this one doesn't work is the latch to hold the lid down tight is broken.  I set weight on it and it was building some pressure but the gauge is finicky too and broken.  I just need a new one.  But when I buy kne I'm getting and electric one.  No burners needed.  Too hot to use propane or any burners jn the RV    Plus mines only 30amp.  So if I have too much going like ACs and the stove. it kicks breaker off.  The electric PC will work great here.    But they are a bit pricey for a decent size one


Edited by Skillzd (07/10/16 06:59 PM)


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Skillzd]
    #23430449 - 07/10/16 08:38 PM (7 years, 6 months ago)

Ok.  So these plates came out much better indeed.  Much less moisture.  Nice smooth finish look on top.    Made a loop with a paper clips.  man is that hard to just get two drops into it.  Three and it falls off to early.  Lol.  Going to make some adjustments.    I'll get it down to a T    It went very well tho compared to the first one.    I also added two layers of plastics over my arm holes.  One layer my hands also go thru a hole that is lower than the Foam holes I have. So it keeps air from getting in around.  Then I put another layer from top to hang over as a dust shield too
.  My OCD is getting to me now since I failed the first time
.  If this time doesn't work ITS ON FOR SURE!!!!  Lol.    I learned ori control my OCD a little since I had no control over my life for a few periods in my life.  So once things bother me it comes out slowly now. Lol


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: Skillzd]
    #23431384 - 07/11/16 07:13 AM (7 years, 6 months ago)

i wouldnt get an electric PC, honestly. they rarely ever hit 15psi, and some i have seen only go to 10(and i kinda doubt they hit it). i have heard lots of bad things about the electric ones.

just buy a nice Presto or Mirro, and hell, if you have the money, get an AA. i have a 22 or 23qt Mirro, which was only $60 with shipping, and I love it.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: mupetmower]
    #23431463 - 07/11/16 07:49 AM (7 years, 6 months ago)

If you want to go electric, go sterilizer :hehehe:


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OfflineSkillzd
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23431639 - 07/11/16 09:39 AM (7 years, 6 months ago)

Ok pasty.  I'll see if I can find one at a decent price.    And yes I knew about the electric ones being shitty.  But the expensive one I used to have went to 20 or 25psi I believe but I was like huge.  And would also blow a breaker I'm sure in my rv here.    So maybe a sterilizer may be better for me.    I'll look into it.        The rv roof Ac doesn't work well.  I have two window units and then my 600w HPS light.  So when I turn any heat on.  I need all three ACs running to keep 75deg or lower in the rv.  So my propane abut we barely gets used.    Worst case scenario I will put it outside for PCing.    If it saves me a lot of money on expensive equipment. 

I was thinking of using this Child's Tent as a Flow hood or Still Air Tent/box.      Do you think it would work good?    It was just an idea.  I have a Aqaurium turned on its side so the open end is toward me.  Took a 1" foam and covered it.  Cut a plus sign for my hands to slip they and then covered the foam with plastic and cut a hole for my hands to fit right in tightly.  But the holes are lower than the foam holes so when sitting there it's closed and no air can get in    And then a third layer just hangs over like a dust shield.        I used it twice and have to re do it.  Only had electrical tape and MicroLite tape.  Duct tap is next.  Lol.  But it's small.  But works good for me cus I need smaller stuff in the RV


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