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OfflineVrayth
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #23399170 - 06/30/16 09:39 PM (7 years, 6 months ago)

Can I sterilize without a pressure cooker? i'll be able to get one in two weeks, but I want to start working on a nice first strain now so Ican go straight to grain once it comes.


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InvisibleJosex
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Re: Pastywhyte's Easy Agar Tek [Re: Vrayth]
    #23400098 - 07/01/16 06:32 AM (7 years, 6 months ago)

Prep the plates as per the tek and steam-sterilize them for 2 hours. I've done it a lot without issues.


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OfflineVrayth
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Re: Pastywhyte's Easy Agar Tek [Re: Josex]
    #23400372 - 07/01/16 08:40 AM (7 years, 6 months ago)

awesome thanks
Quote:

Josex said:
Prep the plates as per the tek and steam-sterilize them for 2 hours. I've done it a lot without issues.




Cool, thanks!


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OfflineR2D2
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Re: Pastywhyte's Easy Agar Tek [Re: Vrayth]
    #23400646 - 07/01/16 10:27 AM (7 years, 6 months ago)

My first attempt using agar! This is about 6 days. I used a multi spore syringe B+. I seem to have some condensation and was wondering if it will be ok? Should I just leave it alone or put it near a fan? I have extra plates for transfers but they have condensation also. Thank you for all the great teks and support! This site is truly amazing.



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Invisiblemupetmower
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Re: Pastywhyte's Easy Agar Tek [Re: R2D2]
    #23400669 - 07/01/16 10:33 AM (7 years, 6 months ago)

yeah, condensation wont be a problem unless its a LOT of it. that one should be fine. try to bring the PC up to temp/pressure slower next time, and also bring it down temp/pressure slower as well.

Im still having issues with condensation with mine, as well, and ive tried bringing the temp/psi up and down literally a slow as i can, and still get quite a bit of it.

i have also noticed that if i make my agar a little more on the thin side(with more water) it seems to get more condensation. so that might be something to think about.

Also, after i mix the agar and pour the plates, i put the lids on top, but dont seal them, and try to let them cool a little first, before sealing. not sure if it would matter either wy, since they will be cooked again inside the PC, but im tryin anything i can think of. It gets so bad sometimes that before i transfer the wedge, there is enough condensation in the receiving plate that it pools, so i can tilt it and drain it out.. so i really dont know haha.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinelukehighwalker710
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23400708 - 07/01/16 10:42 AM (7 years, 6 months ago)

I have also tried bringing it up and down as gently as possible, and let it cool completely before lidding and PCing. Found it to be about the same as if I had just brought it up and down like normal and lidded it little warm. Either way, I get condensation. The mini rounds & squares that I use are worse than the twist lock ones in that department. I don't believe its a problem though.


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OfflineR2D2
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23400716 - 07/01/16 10:45 AM (7 years, 6 months ago)

Awesome! thanks for the replys and good advice. I will slow down my heat up and cool down during the pressure cooking. Great idea to also let the agar cool without completely sealing the lid after cooking it up. I'm as happy as can be that it worked so well. I've had lots of issues with jars and tubs that contaminate and I'm hoping agar and a strong focus on my sterile procedures will get me going in the right direction.


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InvisibleSupalemonhaze
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23400728 - 07/01/16 10:49 AM (7 years, 6 months ago)

Yeah, you can't really do anything about condensation in no-pours. If it pools before you use it, that's good. Use that small pool of water to collect the rest of the condensation and pour it all out before you use it.

Releasing the pressure slowly will do nothing for condensation because the agar is still gonna come out super hot out of the PC. Condensation is a result of a temperature differential between the inside and outside of a container.

If it bothers you that much, you should try pouring agar instead, it is not as hard as most folk seem to think. If you can keep an agar plate clean, you can easily pour your own plates.


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Invisiblemupetmower
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Re: Pastywhyte's Easy Agar Tek [Re: Supalemonhaze]
    #23400763 - 07/01/16 11:00 AM (7 years, 6 months ago)

yeah, i have been thinking about trying pouring plates. gonna get some petris when im back from vacation.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleSupalemonhaze
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23400796 - 07/01/16 11:12 AM (7 years, 6 months ago)

You can pour in PP5s if you want to as well. It's all I've been using lately but obviously, nothing beats a petri dish in terms of the space they take, the time it takes to prep and discard of them and visibility.


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Invisiblemupetmower
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Re: Pastywhyte's Easy Agar Tek [Re: Supalemonhaze]
    #23400831 - 07/01/16 11:21 AM (7 years, 6 months ago)

right, yeah.. ill try it with glad mini's and other pp5s i have first, to get a feel for it, before ruining some petris. so when you pour, you just get the agar out of the PC, still hot(or at least warm) then put it and plates in SAB, and let the agar get to where it isnt hot anymore, and just warm, and still liquid, then just pour, and put tops on plates?

then you let them cool for however long, until solid, and then jut use as normal? as for petri, you put top on, then let cool, then take top back off to put your wedge/whatever in, then top back on, then wrap in cling-wrap, or something, right?

ill look up a tek, just wanted to get the general sense.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisibleSupalemonhaze
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23400867 - 07/01/16 11:32 AM (7 years, 6 months ago)

Yes, more or less.

Don't pour until you are able to handle the bottle by hand without any protection, you will have plenty of time to pour your plates until it cools enough to start to gel. You will notice if time is running out, it will start to thicken before it completely gels on you.

With petri dishes, you split the stack in two, and pour from the bottom up, lifting the entire half stack every time. Once you pour one stack, pour the other. If your SAB allows, you should stack them all on each other so the heat of the ones above will make the bottom ones completely clear of condensation. Also, don't wrap plates which you are not going to use right away, use the sleeve they come in to cover them and put them somewhere where you won't disturb them very often. Even though they are not wrapped individually, the sleeve will protect them enough for contams not to be able to get inside. I've kept plates like this for a couple of months before they started to contam, and the only ones that did were on the top or bottom (forgot which, I think it was the top, strangely enough). The ones in the middle stayed completely clean.


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Invisiblemupetmower
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Re: Pastywhyte's Easy Agar Tek [Re: Supalemonhaze]
    #23400985 - 07/01/16 12:17 PM (7 years, 6 months ago)

awesome, great advice. will do =]


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Offlinedrolman
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Re: Pastywhyte's Easy Agar Tek [Re: mupetmower]
    #23405953 - 07/03/16 01:59 AM (7 years, 6 months ago)

Awesome tek. The microwave tek caused too much condensation for me.  The part about bringing up the temp in the pc  slowly and letting it cool naturally instead of blowing it off has saved me from boiling the agar and having condensation.  Great write up!  Thank you!


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: drolman]
    #23409703 - 07/04/16 09:10 AM (7 years, 6 months ago)

The transfer from the pin seems to have worked out.


This is the plate that I sandwiched, with a tiger drop. Should I transfer from closer to the center on this one?


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Invisibleelasticaltiger
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23411866 - 07/04/16 08:59 PM (7 years, 6 months ago)

You sandwiched it? Like you dropped a plate onto a plate?

Transfer wherever the mycelium looks healthiest.


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Offlineblackout
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Re: Pastywhyte's Easy Agar Tek [Re: drolman]
    #23414364 - 07/05/16 04:55 PM (7 years, 6 months ago)

Quote:

drolman said:
The microwave tek caused too much condensation for me.



A microwave can be used to get rid of excessive condensation water.
https://www.shroomery.org/forums/showflat.php/Number/23149579


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Invisibleherrenvolk


Registered: 05/14/16
Posts: 222
Re: Pastywhyte's Easy Agar Tek [Re: blackout]
    #23419061 - 07/07/16 02:25 AM (7 years, 6 months ago)

Does the micropore tape on these degrade after several PC cycles? Should I be changing the micropore tape each time I make a new batch?


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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: herrenvolk]
    #23419071 - 07/07/16 02:31 AM (7 years, 6 months ago)

If you double layer it you can leave it on at least 5-10 cycles before you need to think about changing it.  I may have never changed it at all on some of them, hard to keep track..


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InvisibleSupalemonhaze
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #23419083 - 07/07/16 02:43 AM (7 years, 6 months ago)

I would change it honestly. It's dirt cheap and easy enough to change, not like having to silicone tyvek or sfds, you know?


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