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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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I tried to transfer the myc. but after a week in the new plates the piece of myc I transfer remain the same as a week before....no expansion...and after few days several green mold start to appear in that plates.... and nothing happened
PS: I used 1.4% of agar for a softer media....and no pour tek. pc for 20/30 min. cooled in SAB wrapped on foil
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things does not exist, everything is a process, so we proceed
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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flame your loop, cool it in an agar plate, touch the print and get just a tiny bit of spores, streak on plate and wait. you gotta use smooth movements inside the box and dont hover your hands over open plates.
maybe what you transfered wasn't healthy myc afterall? post pics if you can. and if you just keep seeing contams and no myc try a new print maybe yours is contamed beyond repair
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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I tried a print and a different syringes....nothing.
Yes the myc wasn't healty...it was bubble but some ppl told me to transfer to clean it...
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things does not exist, everything is a process, so we proceed
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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you transfer only a tiny piece of a clean part of the mycel when transferring from contams, syringes can be a bitch if you use too much on a plate too.
Just try over and over, its a numbers game and eventually you'll get better at it, try to think before you move your arms and do it smoothly. still air is key.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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if nothing else, it will be good practice for your sterile technique. but im sure youll get it eventually. just keep at it, good luck
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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billy jowl
blah



Registered: 12/11/12
Posts: 1,496
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Ive got a question. If i use half pint wide mouths, with plastic lids can i get by without modifying them? I assume i can. Just unscrew 1/4 way while pcing covered with aluminum foil. Then transfer to a sab when cooled. Just like with grain jars.
Sorry if this has already been answered. I missed it if it was. Any advice is very appreciated.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Pretty sure that's how pinpornproducer does it. 
however glass can get slippery. Just keep that in mind.
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billy jowl
blah



Registered: 12/11/12
Posts: 1,496
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Thanks . Also im letting them stiffen up before pcing. Ive read it helps with condensation issues that i haven't been able to avoid as of yet. Dammit..
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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That's silly!!!
I've just innoc.ed a plate of agar....to see if something will grow but after 1 day,1 DAY, I got mold and bacteria contam!! Incredible my friends,after PC I let it cool and keep foiled for 2 week and it was still sterile an clean so is not a pc/sterelization issue. damn I don't know how to deal with that.
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things does not exist, everything is a process, so we proceed
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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its either your sterile technique or spores then. i mean.. im not trying to sound like a dick, but you do know what myc is supposed to look like on agar, right? you can get mold/contam growth while also having myc growth, and then transfer the myc to a new plate.
although, it will usually take spores longer than 24 hours to germinate, so that likely is mold/contam on that plate.
but, to deal with it, all you gotta do is let the myc grow, and as soon as you see growth about the size of a dime, transfer a small wedge off it to a new plate, let it grow to that size again, transfer small wedge, etc, etc, until clean.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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yess I know that I can save the myc....the problem is that myc never grow in any of my attempt...now I'll wait until I have something to save from mold. lool
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things does not exist, everything is a process, so we proceed
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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pics would help.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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He means that he got a contam in just 24 hours, there is no mycelium for him to transfer.
You should make multiple plates in that case, hopefully one will stay clean , even if it's just until you get germination. After that, it's no problem to keep clean.
Have you tried making transfers with blank plates? It will tell you if your sterile technique is adequate.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Quote:
Supalemonhaze said:
Have you tried making transfers with blank plates? It will tell you if your sterile technique is adequate.
ty, and how does this blank plates work?? I mean: what I have to do to test my sterile tek??
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things does not exist, everything is a process, so we proceed
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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PC your plates as normal, and just transfer a wedge from the clean plate to another clean plates. if it contams, then you need to work on your sterile technique. its just to practice and test to see how your technique is.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
esse_jeremy said:
Quote:
Supalemonhaze said:
Have you tried making transfers with blank plates? It will tell you if your sterile technique is adequate.
ty, and how does this blank plates work?? I mean: what I have to do to test my sterile tek??
You are basically doing transfers with agar that has nothing growing on it, no mushroom mycelium, no molds and no bacteria. If you manage to keep those plates clean, it means your sterile tek is good.
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 4 months, 11 days
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So you say to remove the tin foil in the sab. Wouldnt it be okay to remove it before, for example right after you open the P.C.? Aslong as we have the micropore tape shouldnt it prevent contams from entering before we handle the plate? I ended up removing it on two plates i made yesterday but havent to the eight i made today so just wanna know if they are still worth using or should i just clean them out nd reuse them? Thanks -kushroom
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 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pasty Agar Tek [Re: kushroom]
#23384769 - 06/26/16 05:24 PM (7 years, 7 months ago) |
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Quote:
kushroom said: So you say to remove the tin foil in the sab. Wouldnt it be okay to remove it before, for example right after you open the P.C.? Aslong as we have the micropore tape shouldnt it prevent contams from entering before we handle the plate? I ended up removing it on two plates i made yesterday but havent to the eight i made today so just wanna know if they are still worth using or should i just clean them out nd reuse them? Thanks -kushroom
Sure but now there's bacteria and mold spores from open air on top of the plate, greatly reducing your chances of success. One stray bacterium on the edge of a lid rolling off and floating into your work is all it takes. You could just as easily not cut corners and have a sterile lid while making your inoculation, making your work that much easier. It's up to you how high of a success rate you consider acceptable.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Pasty Agar Tek [Re: Inocuole]
#23384810 - 06/26/16 05:36 PM (7 years, 7 months ago) |
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it'll probably be fine. not removing it until SAB is good practice, though.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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kushroom



Registered: 12/04/14
Posts: 588
Loc: I'm lost
Last seen: 4 months, 11 days
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Got ya, thanks for the quick reply guys! Im using a flow hood so i should be okay but i think imma keep the tin foil on from now on, why risk it?
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 All submitted posts are by Someone Who Isn't Me (SWIM) - and in any event are works of pure fiction or outright lies. Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.
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