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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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when you say "with syringes it take long time instead of 3-4 days with print" what do you mean for "long time"?? more that WBS germination time for example?
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things does not exist, everything is a process, so we proceed
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23328713 - 06/10/16 09:27 AM (7 years, 7 months ago) |
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That's just, like, your experience man.

Most likely his prints were much fresher than the syringes he happened to use.
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esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 1 year, 3 months
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Re: Pastywhyte's Easy Agar Tek [Re: dankington]
#23328848 - 06/10/16 10:00 AM (7 years, 7 months ago) |
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I'm scared to death by the idea that my vial is totally shit and I'm waiting for nothing.....
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things does not exist, everything is a process, so we proceed
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
#23328979 - 06/10/16 10:52 AM (7 years, 7 months ago) |
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Quote:
Thoky.ykoht said: Do you think it'd be easier to transfer away from a different starter plate?

So I transfered away from this plate. It now looks like this.

I took two transfers from it when it looked like the original.
 Just wondering how many more transfers until I can noc up some rye. I can spot a small contam at about 10 o'clock on the second plate, noticed it yesterday. I'm planning on transferring to some plates made from munch's 123 guide, see if they perform better on the potatoes then the LME.
Would it be safe to spawn the original transfer plate?
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
#23329002 - 06/10/16 10:59 AM (7 years, 7 months ago) |
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just gotta keep transferring until it comes out clean, before you inoc anything with it. and if your subsequent plates have contam, that were made from transfers from an original, then the original might also have contam.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Pastyplates are basically invincible and can be reused over and over again until time ends and the universe implodes.
I still use them. I've used pour agar too. But I always come back to pastyplates.
Re-usability is the number one benefit to them. Nothing ever needs to be thrown away again.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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I found this bacteria cheat sheet tucked away. Maybe it could be use to some of you.
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D4VE


Registered: 02/06/16
Posts: 2
Last seen: 7 years, 22 days
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I've been reading the forums here for around 9 years or so, but I haven't grown the balls to post to them... until now!
Anyway I am trying to get rid of the need for foil in the PC. So instead of putting holes in the top of the lids I put em on the side under the little overhang tab so water wont drip in. tape a little poly fill over the hole leaving a space at the top and bottom and your done.  Just be careful not to tilt them towards the hole if you take em out while they are still hot. I also started doing this to prepare my bulk subs. Same idea bigger Tupperware. I can stack 3 of these inside my 41.5 qt American standard to make the perfect amount for 1 large monotub. Make sure to add a cooker shelf between each of the big ones or the lids will sink in while the heat is on.
 I've even used these big ones to colonize grain successfully, but the lids do not like to stay on them after they are cooked and it is really sad when a whole big Tupperware full of grain contams. I'm still working on a way to eliminate the need for foil on grain jars. let me know if you guys have any ideas. Im thinking something like this maybe? https://www.stockcap.com/plastic-end-caps.html
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 30 days
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Quote:
elasticaltiger said: Pastyplates are basically invincible and can be reused over and over again until time ends and the universe implodes.
I still use them. I've used pour agar too. But I always come back to pastyplates.
Re-usability is the number one benefit to them. Nothing ever needs to be thrown away again.
A lot of my lids seem to have been warped over time and just slip off with very little force.
I do have a bunch that have survived a few pc cycles so far and are still holding up nicely.
I was thinking maybe when I fill the pc to the brim with pastyplates and the ones on the bottom possibly have too much weight on them ?
I haven't figured out the problem but I would love to use these indefinitely.
I'm in the process of switching to ball baby food jars with this tek because they are so reusable and easy to see through.
If only I could find a clear plastic lid for them then they'd be perfect, but looking through the sides isn't bad at all.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I prefer them after the lids get a little loose, easier to work with IMO. Just don't drop em.
D4ve thats a cool idea on the hole in the side. I may give that a try and see how it does.
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D4VE


Registered: 02/06/16
Posts: 2
Last seen: 7 years, 22 days
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almost forgot... With the hole in the side of the little guys you don't want to let em sit in the water at the bottom of your pc or the polyfill will get wet. I usually put a row of empty jars or jar lids at the bottom to raise em up above the water level.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: Pasty Agar Tek [Re: D4VE]
#23350233 - 06/16/16 08:45 AM (7 years, 7 months ago) |
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I'm on my third transfer, which I switched from LME to PDA.Should I transfer again or am I safe to spawn after letting them grow out a little more?

And the original funky looking plates starting to knot up.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Looks like you've been transferring bacteria too. Pics 1&2 both have some clean looking growth to transfer from though.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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I thought plate two looked like a good one to transfer from as well, idk what my problem is, I keep transferring from what looks like clean growth and keep ending up with bacteria. Where would you transfer from on the first plate. I just think I'm rusty.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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well it can also hide in healthy looking myc for a while, too, even after a few transfers. eventually it will go away with enough transfers, though. especially if you do it like, as soon as you se new growth on the new plates, transfer, then repeat a couple times.
some people do the "agar sandwich" for really clingy, pesky contams.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
Edited by mupetmower (06/16/16 09:29 AM)
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Found another player that just started growing out. Would it be better to transfer from this.

Or stick with plate number two from my previous post.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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why not both, then see which grows best.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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bennylava
Bad example


Registered: 05/29/15
Posts: 587
Last seen: 6 years, 9 months
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How does this actually help? How does this eliminate invisible contams, as he said in the OP.
I mean you still have to do an injection, right?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
bennylava said: How does this actually help? How does this eliminate invisible contams, as he said in the OP.
I mean you still have to do an injection, right?
It helps by providing ample opportunity to transfer away from said contams by forcing them to grow in 2 dimensions rather than 3. There is no explicit need for an injection if you have spore prints available, but even so, it's pretty easy to lift the lid and inject a drop of spore solution before closing the lid back. Most techniques involving pasty plates are covered here:
http://www.shroomery.org/forums/showflat.php/Number/21032261
Contams aren't eliminated, they're just left behind, given good transfer technique.
This one's good too:
http://www.shroomery.org/forums/showflat.php/Number/18430998
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
Loc: out there
Last seen: 6 years, 22 days
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Re: Pasty Agar Tek [Re: Inocuole]
#23374091 - 06/23/16 03:09 PM (7 years, 7 months ago) |
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I let my plates cool in the PC overnight, just brought them into my SAB and took the tinfoil off and most of the filters are wet. Are these plates already trash or can they be used still? Let em dry in SAB? Change the tape? Start over?
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Highwalker
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