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OfflineNDStepp84
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Re: Pasty Agar Tek [Re: mupetmower]
    #23180467 - 05/02/16 02:43 PM (7 years, 8 months ago)

Quote:

mupetmower said:
I picked a couple packs of these up at the local Dollar Tree here:


The red lid ones were a dollar for 5 and the little white lid ones a dollar for 10!!! i hope they both work, i am trying them in the PC now. the red lid ones say pp% on them, so not too worried, but the white lid ones dont say anything. so i hope they are ok. if they work, i am going back to buy every single pack they have. =]



I was eyeballing those same ones at the dollar store that don't say anything on them, a pack of about 8 for a buck. Interested if they will survive,


--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: NDStepp84]
    #23180505 - 05/02/16 02:53 PM (7 years, 8 months ago)

its actually 10 =] and if they dont survive, then i will still be going back to get the red ones which say pp5 on the bottom.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleglobos
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Re: Pasty Agar Tek [Re: mupetmower]
    #23181325 - 05/02/16 06:33 PM (7 years, 8 months ago)

So thanks to this thread I have the Pastywhite agar ready in the fridge and some mycelium fruiting (bought a pre-colonized growbox). Thank you for the beautifully documented tek!

- How do I go about cloning a few fruits? There are so many instructions and I'm information overloaded. A pointer to an easy, tested, trouble-free cloning tek on site would be most welcome.

- After that, is it easier/better to go with a modern BRF tek, or with the birdseed tek recommended by Pastywhite in person? I'm not necessarily after maximum productivity: I strive for minimum engagement and dummy-safe results.

My ultimate goal would be to have liquid cultures and go from there to fruit, in little batches, without sweating it too much and possibly keeping a menagerie of varieties. I think I'm at the beginning of a long trip. Oops, I mean a long voyage of learning to grow!

EDIT
Maybe I should post this question in the Liquid Culture forum or elsewhere?


Edited by globos (05/02/16 06:47 PM)


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: globos]
    #23181358 - 05/02/16 06:40 PM (7 years, 8 months ago)

Quote:

globos said:
So thanks to this thread I have the Pastywhite agar ready in the fridge and some mycelium fruiting (bought a pre-colonized growbox). Thank you!

- How do I go about cloning a few fruits? There are so many instructions and I'm information overloaded. A pointer to an easy, tested, trouble-free cloning tek on site would be most welcome.

- After that, is it easier/better to go with a modern BRF tek, or with the birdseed tek recommended by Pastywhite in person? I'm not necessarily after maximum productivity: I strive for minimum engagement and dummy-safe results.



take a tiny pin and drop it on the plate. thats all you have to do to clone.

2nd question depends on if you have a pressure cooker or not. if not, brf tek. if you do, birdseed.


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: globos]
    #23181362 - 05/02/16 06:41 PM (7 years, 8 months ago)

brf is more dummy-safe, but idk..

you say you got pre-colonized myc? thought that was a myth, honestly.. are you in the states? if so, i dont see how that is cubensis or anything active, then, because it would have been illegal to purchase and mail.

anyways. essentially all you need to do for cloning is take the mushroom you want to clone, put it in SAB, then use your flame-sterilized knife/scalpel to cut a tissue sample from the inner part of the base of the stem. or you can drop a whole pin on agar.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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Invisibleglobos
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Re: Pasty Agar Tek [Re: mupetmower]
    #23181431 - 05/02/16 06:55 PM (7 years, 8 months ago)

Wow! Really quick answers. It's great :sun:
@Munchauzen

Thanks, I do have a PC so I think I'll go with the birdseed tek!

@mupetmower
A myth? It's the only way I ever got mushrooms. Maybe I used the wrong term. Google images mushroom grow box, that's the thing.


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: globos]
    #23181460 - 05/02/16 07:01 PM (7 years, 8 months ago)

oh, so you meant premade sub and you get a spore syringe to inoc it separately, right? i gotcha. but yeah, cloning isnt all that hard to do. take some prints from your grow also, and then you wont have to buy spores again, unless you just want a different variety.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (05/02/16 07:01 PM)


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Offlinejustdopey365
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23181467 - 05/02/16 07:02 PM (7 years, 8 months ago)

Great Tek! I'm happy to see that I can just go to my local grocery store and buy all the items without having to wait for shipping or ordering online. I poked around for a bit to see if someone asked these questions about to ask but I couldn't find them.

New to forum and new to agar.

When I PC them do I PC them at 15 PSI?

After I inoculate the plates do I remove the paper towel and foil?

After inoculation what would be the best location and temperature to store these bad boys?


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: justdopey365]
    #23182096 - 05/02/16 09:50 PM (7 years, 8 months ago)

15 psi is usually standard, some use higher but presto's don't go any higher.
you remove the foil before inoculation, don't replace it. the paper towel is good for taking notes so most leave it on.
store them at room temp pretty much wherever


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineGreenRabbit
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #23182113 - 05/02/16 09:55 PM (7 years, 8 months ago)

Quote:

tryptkaloids said:
15 psi is usually standard, some use higher but presto's don't go any higher.





They do if you make the weight heavier.


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Invisibledankington
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Re: Pasty Agar Tek [Re: GreenRabbit]
    #23182119 - 05/02/16 09:58 PM (7 years, 8 months ago)

Quote:

GreenRabbit said:
Quote:

tryptkaloids said:
15 psi is usually standard, some use higher but presto's don't go any higher.





They do if you make the weight heavier.




I was just thinking about this earlier. But I don't know if I trust the durability of the Presto. I imagine it's only designed for the weight it has, and so I chose not to bc I didn't want to blow my pc. I wonder if I was being overly cautious?


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: GreenRabbit]
    #23182130 - 05/02/16 10:00 PM (7 years, 8 months ago)

so I can add a weight safely? will 20 be more efficient?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetombosley8
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #23182185 - 05/02/16 10:15 PM (7 years, 8 months ago)

I tape a couple quarters to mine to get the psi around 17. I've even been using 3 quarters on 1 and that is close to 20. Haven't done 20 psi with pasty plates just the two quarters.

I would just stick with two quarters to be safe


--------------------


Edited by tombosley8 (05/02/16 10:17 PM)


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OfflineGreenRabbit
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #23182187 - 05/02/16 10:15 PM (7 years, 8 months ago)

Yes, it would be more efficient.

Not sure how safe it is. I wouldn't be too worried if you kept it at 20.

I'm not liable if you do something stupid.


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Invisiblemupetmower
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Re: Pasty Agar Tek [Re: mupetmower]
    #23183388 - 05/03/16 08:59 AM (7 years, 8 months ago)

Quote:

mupetmower said:
I picked a couple packs of these up at the local Dollar Tree here:


The red lid ones were a dollar for 5 and the little white lid ones a dollar for 10!!! i hope they both work, i am trying them in the PC now. the red lid ones say pp% on them, so not too worried, but the white lid ones dont say anything. so i hope they are ok. if they work, i am going back to buy every single pack they have. =]




both types of these containers worked perfectly. PC'd for an hour at 15psi and neither warped even in the slightest. so i am gonna go buy every last pack of each of these. =] i especially like the smaller ones, because i can use these until i get a clean culture and then transfer it to the bigger ones for when i want to inoc something with the culture. im so happy i found these =]

EDIt - also, i forgot to say, both of these types of containers had WAAAAAAY less condensation then the glad mini rounds and the ziplock square ones. idk why, because i did all 4 types with the saem batch of agar at the same time, and in the same PC cycle and everything.. and i brought the PC up to temp and pressure as slow as i could, and then let it cool very slowly, and then left it to cool further over night in the PC, unopened. so i dont know why, but they make much less condensation. so im stocked about these working so well, and survivng the PC!!


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (05/03/16 09:02 AM)


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Invisibleglobos
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Re: Pasty Agar Tek [Re: mupetmower]
    #23184363 - 05/03/16 01:07 PM (7 years, 8 months ago)

Quote:

mupetmower said:
oh, so you meant premade sub and you get a spore syringe to inoc it separately, right?




No, it was already inoculated. Mycelium roaming wild - only need to dunk :smile:

Quote:

take some prints from your grow also, and then you wont have to buy spores again, unless you just want a different variety.




A bit late for that. My fruits have ripped the veil and now it's all covered in spores. I feel guilty to hijack this thread (which is about Pastywhite's tek after all), so I posted a question like "which teks do I follow now?" in the newbie forum. Here it is, if anyone feels they can help.
https://www.shroomery.org/forums/showflat.php/Number/23184326

Thanks a lot :grin:


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Offlinejustdopey365
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #23192214 - 05/05/16 12:36 PM (7 years, 8 months ago)

Hello Pastywhyte,

I have followed your instructions and have inoculated my glad minis. I have a storage question for you. Right now I have them in a kitchen covered shelf. Does it matter if I give them sunlight or not? Does sunlight promote growth and help the mycelium fight contams? Also, keep them in a temperature zone of 65-75 F?

Thank you,

-Dopey


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: justdopey365]
    #23193129 - 05/05/16 05:49 PM (7 years, 8 months ago)

sunlight isn't a big deal. that is an ideal temp range


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisibleforagedfungus
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Re: Pasty Agar Tek [Re: mupetmower]
    #23193176 - 05/05/16 06:08 PM (7 years, 8 months ago)

Quote:

mupetmower said:
I picked a couple packs of these up at the local Dollar Tree here:


The red lid ones were a dollar for 5 and the little white lid ones a dollar for 10!!! i hope they both work, i am trying them in the PC now. the red lid ones say pp% on them, so not too worried, but the white lid ones dont say anything. so i hope they are ok. if they work, i am going back to buy every single pack they have. =]




I use those little white lidded ones. I really like 'em. They're so much smaller than mini-rounds. I can fit 60+ in the pc (14 qt AA) at once. So I only need to make dishes every month or two, instead of every week! They take up way less room storing plates in the fridge too.


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Offlinejustdopey365
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #23193971 - 05/05/16 10:27 PM (7 years, 8 months ago)

Thank you for your help!


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