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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I have limited space too but there's always room for one more tub.
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cheap thrills
grinner
Registered: 10/24/15
Posts: 29
Loc: the corner
Last seen: 5 years, 4 months
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#22772965 - 01/13/16 01:44 AM (8 years, 18 days ago) |
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Ola. TBH I didnt read all 204 pages of this thread. But have read the TEK on the first page. I dont have a PC and was wondering if it could be done in a pot and steam sterilized? I have also looked into Violets Microwave Agar TEK and might try that, but preparing each pp5 individually seems like a lot of work. I like the idea of preparing 'bulk' agar, then pouring, then sterilizing.
Any tips, help, ideas, are most welcome.
Thanks Pastywhyte!
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Violet



Registered: 12/06/11
Posts: 4,205
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I've been told that agar can be steam sterilized and for the most part I believe it.
I'm not entirely sure on your statement about microwaving being a lot of work. You say you want to prepare your agar, then distribute it into the containers, then sterilize the containers, right? Do you think that this isn't how the microwave tek works as well? That's gonna be pretty much all no-pour agar methods. The only thing with the microwave sterilization is that you use extra water in the recipe to account for water loss, and you run them one at a time (at least at first until you understand it), which really is not a lot of work.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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cheap thrills
grinner
Registered: 10/24/15
Posts: 29
Loc: the corner
Last seen: 5 years, 4 months
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
#22773019 - 01/13/16 02:09 AM (8 years, 18 days ago) |
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Quote:
Violet said: I'm not entirely sure on your statement about microwaving being a lot of work. You say you want to prepare your agar, then distribute it into the containers, then sterilize the containers, right? Do you think that this isn't how the microwave tek works as well? That's gonna be pretty much all no-pour agar methods. The only thing with the microwave sterilization is that you use extra water in the recipe to account for water loss, and you run them one at a time (at least at first until you understand it), which really is not a lot of work.
Hey V hope that you didn't take offence. Please see my post on your Agar TEK, My concern is splitting the Agar to 0.5g per pp5 as I dont have a scale. Was hoping I could make enough for a few at once then only split the agar grainwater mix into the individual containers. Doing the containers individually in the microwave isn't my concern.
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Violet



Registered: 12/06/11
Posts: 4,205
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I took no offense of course, it seemed like maybe that was what you meant. I did respond there too. Just keep in mind if you use these glad containers you need to leave the lids totally unsnapped, and you have to have a proper filter on the lids so you can snap the lid shut when you open the microwave. That way any air drawn into the container goes thru the filter, stopping contams from being pulled in, which in screw-top containers is done by the lid, the threads, and the loose seal.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
#22773564 - 01/13/16 07:24 AM (8 years, 18 days ago) |
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Quote:
Violet said: . Just keep in mind if you use these glad containers you need to leave the lids totally unsnapped, and you have to have a proper filter on the lids so you can snap the lid shut when you open the microwave. That way any air drawn into the container goes thru the filter, stopping contams from being pulled in, which in screw-top containers is done by the lid, the threads, and the loose seal.
so now your saying that lid threads filter air, dear god..
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Pastywhyte's Easy Agar Tek [Re: 13shrooms]
#22773945 - 01/13/16 10:06 AM (8 years, 17 days ago) |
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No I didn't. That's distorting my words. But I have done countless of them without a single contam getting in. As have Pasty, Muda, Machi, cron, and many many others.
The lids stopping contaminants from getting in is not a new thing I'm "now" saying. I've been saying it for years, and it's been tested tried and true Its catching on is largely responsible for the likes of Pasty's post about using unmodified plastic lids for glass jars. The lids themselves keep contams out. You can try to distort, skew, and ridicule it, but you only end up putting yourself on the side against truth.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
Edited by Violet (01/13/16 10:56 AM)
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
#22773979 - 01/13/16 10:18 AM (8 years, 17 days ago) |
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It used to make me really nervous but it's never been a problem.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#22774346 - 01/13/16 12:25 PM (8 years, 17 days ago) |
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I'm more concerned about it in the microwave where the most rapid and intense air intake will take place than in the PC where things have a good chance to cool off to a more reasonable level.
I need to do testing with twisttops in the microwave. I've only done filtered poptops so far. The air may be pretty dead in there from all the microwaving, but there is also a fan.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Markmorbidity
NooB



Registered: 08/07/14
Posts: 103
Loc: Pallet Town
Last seen: 7 years, 3 months
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1st time noob here.... Trying agar for the qst time. I dont have karo or honey. I used sugar.... Would that be fine?
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Violet



Registered: 12/06/11
Posts: 4,205
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Karo is cheap. Honey isn't too expensive, and has many wonderful uses. Go spend a few bucks and get the right thing.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Pasty Agar Tek [Re: Violet]
#22774465 - 01/13/16 01:11 PM (8 years, 17 days ago) |
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It will probably work, especially since potato is the main nutrition. I think a general rule of thumb with this shit is to use the most complex sugars you have available. Things like malt, maybe a drop of molases if you've got it, brown sugar, maple, honey, karo. Probably the more you mix together the better.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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sugar in the raw brand sugar works.
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Markmorbidity
NooB



Registered: 08/07/14
Posts: 103
Loc: Pallet Town
Last seen: 7 years, 3 months
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Igot packets of those sugar in the raw. Ill get karo tomorrow since i get paid.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Yep, splurging a paycheck on syrup is how kings live. Welcome to the throne.
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Piaseski
Day Tripper
Registered: 11/09/15
Posts: 391
Last seen: 1 month, 14 days
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Quote:
cheap thrills said:
Quote:
Violet said: I'm not entirely sure on your statement about microwaving being a lot of work. You say you want to prepare your agar, then distribute it into the containers, then sterilize the containers, right? Do you think that this isn't how the microwave tek works as well? That's gonna be pretty much all no-pour agar methods. The only thing with the microwave sterilization is that you use extra water in the recipe to account for water loss, and you run them one at a time (at least at first until you understand it), which really is not a lot of work.
Hey V hope that you didn't take offence. Please see my post on your Agar TEK, My concern is splitting the Agar to 0.5g per pp5 as I dont have a scale. Was hoping I could make enough for a few at once then only split the agar grainwater mix into the individual containers. Doing the containers individually in the microwave isn't my concern.
I don't have scales either, and just wanted to mess about with my Galindoi, after PCing using this TEK i decided to try a kind of version of Violets microwave tek.
I used 250ml grain water to 250ml tap water, drop of honey and 2 level teaspoons of agar...very rough approximations just doing it by eye.
Boiled it up, added food colouring, set it in a jar in the fridge.
When i want to make a plate i take one of my half pint pp5s, simple polyfill hole in lid.
Scrape a big tablespoon of my set agar mixture into pp5, microwave on Defrost setting with the lid resting on top (equilivent to the 30% i guess, goes on and off as Violet tek states) for about 7-8 minutes.
Allow it to cool down a bit in microwave without opening the door, then i reach inside and quickly click the lid down in to place.
No contam issues yet, fair bit of condensation, tiny bit of water on surface but nothing more than i was experiencing with the PC, i just tip it out in the SAB before transfering.
This was made up 2 weeks ago, and inoculated (with a shakey hand, as you can see the transfer bounced and created 2 germination sites) like 5 days ago;
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Edited by Piaseski (01/13/16 04:41 PM)
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Lemon Hope
Hope

Registered: 01/05/16
Posts: 257
Loc: WA
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Re: Pastywhyte's Easy Agar Tek [Re: Piaseski]
#22784410 - 01/15/16 11:12 PM (8 years, 15 days ago) |
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I can only find the screw top pp5's. I won't be doing a ton of plates but it's all they had. Went six different places. Shouldn't matter much any at all right? So long as it's sterile and done in still air it shouldn't matter? The twisting and turning will cause a lot of air to move around.
This is the closest kind of medical tape next to 3M micropore of which I could not find anywhere at all. Should be fine right?
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Pastywhyte's Easy Agar Tek [Re: Lemon Hope]
#22784453 - 01/15/16 11:24 PM (8 years, 15 days ago) |
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Twisttops are great, you don't need to modify them at all. Just cook with it slightly untwisted then shut it as you remove it from the PC. Violet was doing this method before this tek was released and still does it. Be aware, the bottom of a PP5 pint (I'm guessing that's what you got) will need a bit more agar than pasty's recipe suggests. Also, you need to tilt your container a bit more to keep your hands from getting over it due to the high walls. That's good practice anyway incase contams are falling of your tools.
Again, don't modify the lids at all, just leave them slightly loose during the PC run so they don't get crushed. Otherwise agar plates don't need GE, especially with a pint of air in there.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Lemon Hope
Hope

Registered: 01/05/16
Posts: 257
Loc: WA
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Nice, thanks so much! One more item and it's lift off.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: Pastywhyte's Easy Agar Tek [Re: Lemon Hope] 1
#22785481 - 01/16/16 07:33 AM (8 years, 15 days ago) |
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Nexcare micro pore tape is fine. It just leaves adhesive behind when you try to peel it off to replace. 3M's doesn't which is just a convenience.
Twisting the lid won't stir up too much as long as your fluid in your motions. They'll work well as a substitute.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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