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InvisibleInocuole
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #22668207 - 12/17/15 03:51 PM (8 years, 1 month ago)

Quote:

stareatclouds said:
And don't use your lighter inside the SAB. You're asking to ignite fumes and go to the ER.



For the love of fuck, this.
http://www.shroomery.org/forums/showflat.php/Number/22586071


Guy removed the pictures but you get the idea.  They were gruesome btw.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
    #22668318 - 12/17/15 04:16 PM (8 years, 1 month ago)

Yeah that was nasty. I lit my arms on fire once but that's way different than blowing up a plastic box of flammables.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #22668372 - 12/17/15 04:34 PM (8 years, 1 month ago)

Remove the gloves and get some arm sleeves is the way to go huh? Haha I've been using the lighter alcohol combo with that box for years and years! Never had a problem.. Perhaps I'm just lucky. But yeah I've seen people on here throughout the years spraying down the inside of their box with Lysol n shit then using a lighter in it.. I just have some swabs in there. Still pretty dangerous I suppose.

Anyway, just got done with the cloning. Used some inside stipe tissue, just kept splitting the step more and more for each consecutive plate to get a hopefully somewhat clean sample. Ran out of stem so I started splitting the cap. Guess we will wait and see if anything happens!


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22668425 - 12/17/15 04:48 PM (8 years, 1 month ago)

Guess I'll be using soapy water from now on, thanks for the link :lmafo:


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OfflineSivarted
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22668477 - 12/17/15 05:05 PM (8 years, 1 month ago)

Soapy water...

I've been wondering about this because I'm getting tired of filling my lungs with bleach or lysol.  What kind of soap do folks use, and in what concentration?  Ideally something that wouldn't suds up so much that the sides of the SAB are useless for seeing through.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Sivarted]
    #22668563 - 12/17/15 05:26 PM (8 years, 1 month ago)

Quote:

Sivarted said:
Soapy water...

I've been wondering about this because I'm getting tired of filling my lungs with bleach or lysol.  What kind of soap do folks use, and in what concentration?  Ideally something that wouldn't suds up so much that the sides of the SAB are useless for seeing through.




http://www.shroomery.org/forums/showflat.php/Number/22586071


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OfflineSivarted
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22668602 - 12/17/15 05:36 PM (8 years, 1 month ago)

Quote:

CapMeh said:
Quote:

Sivarted said:
Soapy water...

I've been wondering about this because I'm getting tired of filling my lungs with bleach or lysol.  What kind of soap do folks use, and in what concentration?  Ideally something that wouldn't suds up so much that the sides of the SAB are useless for seeing through.




http://www.shroomery.org/forums/showflat.php/Number/22586071



Yeah I saw that thread.  Never used ISO in my SAB.

But I don't want to use bleach anymore cause it fills my bedroom with fumes.  So what kind of soap?  Dishsoap?  Pinesol?  Does it matter?  Lol


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: Sivarted]
    #22668608 - 12/17/15 05:39 PM (8 years, 1 month ago)

doesn't matter. don't worry about being so precise, in general. this is amateur mycology, its supposed to be simple and accessible.


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OfflinetaGyo
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Re: Pastywhyte's Easy Agar Tek [Re: Sivarted]
    #22668638 - 12/17/15 05:45 PM (8 years, 1 month ago)

Quote:

Sivarted said:
Quote:

CapMeh said:
Quote:

Sivarted said:
Soapy water...

I've been wondering about this because I'm getting tired of filling my lungs with bleach or lysol.  What kind of soap do folks use, and in what concentration?  Ideally something that wouldn't suds up so much that the sides of the SAB are useless for seeing through.




http://www.shroomery.org/forums/showflat.php/Number/22586071



Yeah I saw that thread.  Never used ISO in my SAB.

But I don't want to use bleach anymore cause it fills my bedroom with fumes.  So what kind of soap?  Dishsoap?  Pinesol?  Does it matter?  Lol



Regular dish soap works well :thumbup:


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #22668641 - 12/17/15 05:45 PM (8 years, 1 month ago)

On the first page bodhisatta says you can use 500ml+ 1drop dish soap.


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OfflinePsilosoulful

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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22668711 - 12/17/15 06:00 PM (8 years, 1 month ago)

Yeah that'll work. Make sure to shake it up really well. A few extra drops wouldn't hurt tho.


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OfflineDistrict
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Re: Pastywhyte's Easy Agar Tek [Re: Psilosoulful]
    #22669567 - 12/17/15 08:43 PM (8 years, 1 month ago)

Anyway to remove condensation from the glass petris after they formed asides from wiping them down with towel?


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OfflinePreparationH
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Re: Pastywhyte's Easy Agar Tek [Re: District]
    #22669805 - 12/17/15 09:30 PM (8 years, 1 month ago)

I transferred from 2 plates to 8 today.  Think it went well, getting the fuckin agar off my exacto knife was a bigger pain in the ass that I was expecting .  The plates I was cutting were all poured to one side because they tipped over in the PC though which made it tough.


What happens if you PC plates and remove them right away from the PC?  Not gonna fuck it up right?


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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH]
    #22669832 - 12/17/15 09:33 PM (8 years, 1 month ago)

no. I remove them immediately when the pressure reaches zero but they are still hot as hell and the agar is liquid. let them cool on the counter for nice level plates.


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OfflinePreparationH
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #22669863 - 12/17/15 09:39 PM (8 years, 1 month ago)

Yea that's why I removed them, the first time a bunch of plates were in the corner and a pain in the ass to cut out tonight.  Whatever we'll see how it goooooes


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OfflineDistrict
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Re: Pastywhyte's Easy Agar Tek [Re: PreparationH]
    #22670694 - 12/18/15 03:15 AM (8 years, 1 month ago)

Quote:

PreparationH said:

What happens if you PC plates and remove them right away from the PC?  Not gonna fuck it up right?




As opposed to waiting for it cool, I get condensation on glass and in the agar.


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OfflineCapMeh
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #22671423 - 12/18/15 10:49 AM (8 years, 1 month ago)

Quote:

stareatclouds said:
And don't use your lighter inside the SAB. You're asking to ignite fumes and go to the ER.




Have I been wrong this whole time to think you need to flame sterilize your needles and other utensil in your SAB between jars/plates or whatever else to avoid cross contamination? Id rather have a lighter in there than alcohol swabs.. Or are you just saying don't use a lighter in combination with ISO in a SAB?

Id like to hear what box setup some of you use. Does this sound like an okay setup: disposable gloves, tyvek sleeves, inside sprayed with soapy water, lighter inside SAB?


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Invisiblestareatclouds
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22671446 - 12/18/15 10:54 AM (8 years, 1 month ago)

You need to flame sterilize your needle in between inoculations if your needle touches agar. A loop or scalpel will always touch, so yes, flame sterilize in between. You don't bring your lighter into your SAB though.

I take my left hand out of the SAB to hold my flame maybe 5" away from the hole and use my right hand, inside the SAB, to stick out my syringe/scalpel/loop to sterilize it.


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Invisibleinsanemike
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22671447 - 12/18/15 10:55 AM (8 years, 1 month ago)

Lighters suck for flame sterilization. I would either buy a propane torch or an alcohol lamp. Denatured alcohol is pretty cheap.


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OfflineNDStepp84
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Re: Pastywhyte's Easy Agar Tek [Re: CapMeh]
    #22671529 - 12/18/15 11:23 AM (8 years, 1 month ago)

Quote:

CapMeh said:
Quote:

stareatclouds said:
And don't use your lighter inside the SAB. You're asking to ignite fumes and go to the ER.




Have I been wrong this whole time to think you need to flame sterilize your needles and other utensil in your SAB between jars/plates or whatever else to avoid cross contamination? Id rather have a lighter in there than alcohol swabs.. Or are you just saying don't use a lighter in combination with ISO in a SAB?

Id like to hear what box setup some of you use. Does this sound like an okay setup: disposable gloves, tyvek sleeves, inside sprayed with soapy water, lighter inside SAB?



Definitely best to flame outside the box. Other than being dangerous if you are working ISO, flames inside will stir up the the air. Ever blow out a smoke cloud and strike a lighter under it? You can see what a flame does to still air.

I don't use tyvek sleeves, just a tote with arm holes cut out sprayed with soapy water. Turn off central air/ fans. I shower and put freshly laundered clothes, gloves and mask.  Everything going in is wiped down with Iso, my hands and arms as well.


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
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Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag


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