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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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I'm still feeling like a noob compared to most of the TCs and a good few of the non-TCs, but it's liberating being able to do things however you want. Past the basics, it's easy to customize the hobby to your strengths.
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blackdust
Registered: 02/28/09
Posts: 8,327
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Re: Pasty Agar Tek [Re: Inocuole]
#22303655 - 09/28/15 04:08 PM (8 years, 6 months ago) |
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anyone can bake cookies from a recipe How many people actually understand the reasons for the recipe though? at a certain point in this hobby, everyone's thoughts on which recipe to use become shit.
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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If I inoculate something and leave that agar for 2 1/2 weeks I'll have a pin on that agar if I didn't use super high nutes/dump a shit ton in, etc. Take that sterile pin, drop it on another agar plate, wait for that to grow out and then LI.
Pinning isn't genetic so much as us getting thirsty is not genetic, it's just all over time. Even the slowest, weakest culture will pin if left on agar for a long enough time.
I bet some of the best cultures didn't come from a pin on agar. Pins on agar are better then MS though.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Ajahn Don
Stranger
Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 11 months, 18 days
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Re: Pasty Agar Tek [Re: taGyo]
#22304147 - 09/28/15 05:33 PM (8 years, 6 months ago) |
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So there may be superior cultures out there started with MS, but the greater likelihood is with pins from agar? This KSSS cube I'm working with is a pin from a pin in vitro from a MS syringe. Maybe it's just lucky. We are pretty happy with this, since it's a first-time experience. Chasing the genes.
PS: I get thirsty from smokin'.
-------------------- "He's not altogether dense, but he's not altogether there."
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Ajahn Don
Stranger
Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 11 months, 18 days
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Re: Pasty Agar Tek [Re: Inocuole]
#22304158 - 09/28/15 05:35 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: I'm still feeling like a noob compared to most of the TCs and a good few of the non-TCs, but it's liberating being able to do things however you want. Past the basics, it's easy to customize the hobby to your strengths.
But when you look back, do you not experience pleasure at how far you've come?
-------------------- "He's not altogether dense, but he's not altogether there."
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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If I had a culture and it pinned on the plate I'd fruit both plates and see the difference. Then clone a cluster.
I look back at youthful, fun-loving, naive, I'm-gonna-make-LCs-from-spores Tagyo and I go "How little you know young one... How little you know."
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: taGyo]
#22304217 - 09/28/15 05:49 PM (8 years, 6 months ago) |
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Yeah... I guess it's nice not tripping over my own feet all the time, but it never ends really.
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Ajahn Don
Stranger
Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 11 months, 18 days
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Re: Pasty Agar Tek [Re: Inocuole]
#22304615 - 09/28/15 07:05 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: Yeah... I guess it's nice not tripping over my own feet all the time, but it never ends really.
It's also pretty nice to rebound from my mistakes. Doesn't take long to get smarter, but it requires the effort. Those of us who find this a passion are lucky.
And truly, it's amazing at how many people caught fire when Pasty made a plate.
Is there anyone out there not convinced? Petris make me feel like a mad scientist, but Pasty Plates make me feel the counter-culture of my youth.
-------------------- "He's not altogether dense, but he's not altogether there."
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,844
Loc: Canada
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Quote:
Ajahn Don said: And truly, it's amazing at how many people caught fire when Pasty made a plate.
Shit man, no one was more blown away than me. . .
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13shrooms
Lightning Shaman
Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Quote:
Ajahn Don said:
Quote:
Inocuole said: Well then..... Cant beat spore to fruit in like 2 1/2 weeks.
I don't really know how that can even happen, except I have to change the way I think about mushrooms. They aren't like plants which have a growing cycle from seed to fruit. Maturity in cubes doesn't seem to work like animals or plants. They may not even store energy, but fruit when they are under siege, and fruit when they are most happy.
However, I'm betting on it being genetic to pinning. Hell, we might not even know what a mushroom is intending when it pins. I'm listening, but what are they saying?
then whats this? (being facetious)
from Stamets book,
they soo have a life cycle.
-------------------- A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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abductee
Time
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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I ended up finding that book at a decent price, it should arrive by my bday. A lot of new ones were 90 bucks Canadian..
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: abductee]
#22307634 - 09/29/15 11:23 AM (8 years, 6 months ago) |
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Well when you read it, just remember to ignore anything that you've already learned better here.
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abductee
Time
Registered: 05/07/15
Posts: 2,224
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#22307717 - 09/29/15 11:45 AM (8 years, 6 months ago) |
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Will do.
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: abductee]
#22309251 - 09/29/15 04:04 PM (8 years, 6 months ago) |
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Ah, about agar pins, I just noticed that the pasty plates with SFDs don't like to pin as much as the ones that use micropore or poly, or a mix of both. Probably nearly enough air exchange to count as FAE.
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: Inocuole]
#22309293 - 09/29/15 04:09 PM (8 years, 6 months ago) |
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Quote:
Inocuole said: Ah, about agar pins, I just noticed that the pasty plates with SFDs don't like to pin as much as the ones that use micropore or poly, or a mix of both. Probably nearly enough air exchange to count as FAE.
Tyvek suit filters work great for agar pins also.
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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I've been using the same tyvek suit since I started. Now I have SFDs and I'm probably going to donate it to some noob.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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insanemike
Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: taGyo]
#22309399 - 09/29/15 04:21 PM (8 years, 6 months ago) |
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I've ran jars and pasty's with both tyvek suit filters and sfd's and have to say that the price and convenience of buying the suits far out weighs having the same performance, higher price and less convenience when buying sfd's from the internet.
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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For jars I lean toward the SFDs, for pasty plates I could see tyvek being good.
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taGyo
Strainiac/AMU
Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 6 months
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Re: Pasty Agar Tek [Re: Inocuole]
#22309460 - 09/29/15 04:30 PM (8 years, 6 months ago) |
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I probably PC something every other day. I cut my sfds to quarter size so I get about 10-12 jars per circle. For me the fee starts to make sense when I'm not cutting tyvek suits anymore and just stickin' foil on top and throwin it in.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Boomertown
Psychonaut
Registered: 06/21/15
Posts: 183
Last seen: 5 years, 7 months
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Re: Pasty Agar Tek [Re: taGyo]
#22321061 - 10/01/15 08:34 PM (8 years, 6 months ago) |
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I am about to give up on agar.....i don't understand how to get rid of said "mold" the fluffy white shit. I've done many transfers now and to no avail. Now I seem to be getting trich in half of the plates I do and the other half just suck. I use a SAB...I wipe down my plates with iso. I flame my scalpel after each transfer. I have my plates upside down. I always transfer from the best looking outside edge....I just don't get what I am doing wrong.
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