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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #22302162 - 09/28/15 10:37 AM (8 years, 4 months ago)

Feeling rather proud this morning. My first pins on agar just appeared yesterday.



These are on a plate of KSSS from a cloned pin which appeared in a brf jar. This is the second transfer. My theory is the better the genetics, the more likely to pin on agar. Fair guess?


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Offlinekosmokratorshaman
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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22302324 - 09/28/15 11:16 AM (8 years, 4 months ago)

does mycelium often pin on agar? I have my first mycelium showing on agar. My other plates were serious contammed. I grew them out anyway, to learn some differences.


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InvisibleBlueIndian
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Re: Pasty Agar Tek [Re: kosmokratorshaman]
    #22302376 - 09/28/15 11:28 AM (8 years, 4 months ago)

Ok the square ziploc brand I got didn't work out so well so I picked up some of the round Glad ones. Since the lids seem to be really tight brand new should I put some water in them and microwave them a few cycles with the lids cracked to loosen them up some? I can see it being a struggle to get the lids off in a SAB as they are...


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: BlueIndian]
    #22302508 - 09/28/15 11:59 AM (8 years, 4 months ago)

Quote:

Ajahn Don said:
Feeling rather proud this morning. My first pins on agar just appeared yesterday.



These are on a plate of KSSS from a cloned pin which appeared in a brf jar. This is the second transfer. My theory is the better the genetics, the more likely to pin on agar. Fair guess?



It's not better genetics, it's just a culture that likes to pin. Potency, total dry weight, etc. are all things that come into play in designating something as a good culture.

Quote:

kosmokratorshaman said:
does mycelium often pin on agar? I have my first mycelium showing on agar. My other plates were serious contammed. I grew them out anyway, to learn some differences.



It's not often and when it does happen pick the pin off one plate and drop it on another and fruit that and see whatcha got. Pins tend to have aggressive mycelium that wants to fruit.

Quote:

BlueIndian said:
Ok the square ziploc brand I got didn't work out so well so I picked up some of the round Glad ones. Since the lids seem to be really tight brand new should I put some water in them and microwave them a few cycles with the lids cracked to loosen them up some? I can see it being a struggle to get the lids off in a SAB as they are...



I would just run them through the PC for a while with water in them. Eventually they loosen up. Some of my glad mini rounds are so easy to pop off it's ridiculous after the second PC cyce. Still stay on firmly when I hold them upside down though but a stick of melted butter could pry open some of them.

Try your microwave idea as well but I'd just PC them once or twice until they're acceptable. Usually after the first PC cycle they loosen up considerably.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: taGyo]
    #22302539 - 09/28/15 12:09 PM (8 years, 4 months ago)

What do you mean it's not often?



I'm more surprised when agar DOESN'T pin.


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Inocuole]
    #22302683 - 09/28/15 12:52 PM (8 years, 4 months ago)

Contrary to what you have there I think more plates end up not pinning then do unless left out with the intention of having it pin.

Most people go through cultures real quickly and don't give it that time, you were mentioning before that you specifically lay out plates and give some light for pins.

A little different :lol:


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Re: Pasty Agar Tek [Re: taGyo]
    #22302699 - 09/28/15 12:54 PM (8 years, 4 months ago)

Well nothing's going to pin before full colonization, which is usually not a goal of an agar plate.  You're either using it normally and not encountering pins because you throw it away after a transfer, or you let shit sit out after you're done with it and it pins.  I mean...  There aren't a ton of variables here.  You let it pin or you don't. :shrug:


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Inocuole]
    #22302710 - 09/28/15 12:55 PM (8 years, 4 months ago)

Exactly,

Which seems like it's backing up my original statement: I think more plates don't pin then do.

When I quit for a while there I had plates that I left up in my storage section. I came back to fully mature, albeit skinny and weak, mushrooms.

I ate them and continued on with my move :laugh2:


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: taGyo]
    #22302959 - 09/28/15 01:43 PM (8 years, 4 months ago)

Those pins grew on pasty plates inoculated 9/13. They are the only ones which have done that in all the time I've been growing them. I have two other pasties from the same transfer which did not pin.

Just sayin'.

I transferred the two pins onto pasties of their own, to run beside the KSSS jars I have colonizing and about to be spawned. One of these is going to be my best so far.


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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22302974 - 09/28/15 01:46 PM (8 years, 4 months ago)

Well I'm sure genetics play a huge role, but like 7/10 of the plates that I leave after I make transfers pin within a week or two.  Maybe I'm just lucky, maybe I'm doing something different.


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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22302983 - 09/28/15 01:48 PM (8 years, 4 months ago)

:awesome2:


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Inocuole]
    #22302993 - 09/28/15 01:50 PM (8 years, 4 months ago)

What was that last part? And the first part? Like one or the other?

Quote:

Inocuole said:
Well I'm sure genetics play a huge role, but like 7/10 of the plates that I leave after I make transfers pin within a week or two.  Maybe I'm just lucky, maybe I'm doing something different.




:lmafo: Ain't no luck to it.


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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22303062 - 09/28/15 02:06 PM (8 years, 4 months ago)

Well then.....  I dunno why my PE, APE, AA+,  and Golden Halo all pin reliably on agar.  KSSS was the only one not to but it seemed contaminated.  Cant beat spore to fruit in like 2 1/2 weeks.


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Re: Pasty Agar Tek [Re: Inocuole]
    #22303089 - 09/28/15 02:13 PM (8 years, 4 months ago)

I'll say if I leave a plate around it pins but in the regular process of doing things if a pin pops on a plate I was fucking around with then I clone that. My first pin was KSSS and that pinned the day after it fully colonized. Never got to try that fucking culture though.

My shortest plate to pin time was within a week after full colonization left at 25c. I say within a week 'cause it was my birthday and I went to AC.


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Re: Pasty Agar Tek [Re: Inocuole]
    #22303258 - 09/28/15 02:44 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Well then.....  I dunno why my PE, APE, AA+,  and Golden Halo all pin reliably on agar.  KSSS was the only one not to but it seemed contaminated.  Cant beat spore to fruit in like 2 1/2 weeks.




sounds like you just wait too long b4 using them.  I use plates as soon as there is enough growth to transfer :shrug:

if you want a spore print quick then fine or want to clone the fast first pin cool.  :stoner:


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Re: Pasty Agar Tek [Re: 13shrooms]
    #22303288 - 09/28/15 02:55 PM (8 years, 4 months ago)

I said specifically that these are plates I leave after a transfer.  I just don't throw them away because I don't need more plate immediately so they sit by my lights. I'm not letting the myc overrun the plate because I'm not transferring, if you thought that was the issue.  :shrug:  Give me some credit man, I know better than that.


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Inocuole]
    #22303295 - 09/28/15 02:56 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Well then.....  Cant beat spore to fruit in like 2 1/2 weeks.




I don't really know how that can even happen, except I have to change the way I think about mushrooms. They aren't like plants which have a growing cycle from seed to fruit. Maturity in cubes doesn't seem to work like animals or plants. They may not even store energy, but fruit when they are under siege, and fruit when they are most happy.

However, I'm betting on it being genetic to pinning. Hell, we might not even know what a mushroom is intending when it pins. I'm listening, but what are they saying?


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22303306 - 09/28/15 02:57 PM (8 years, 4 months ago)

I know these plates didn't sit around waiting for a pin. 14 days, they pinned. Since I've never had a pin grow from agar, and was once skeptical until I saw Noc's pictures, these certainly weren't being grown for pins.


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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22303310 - 09/28/15 02:58 PM (8 years, 4 months ago)

Well, recently, with golden halo, I took spores from a swab, got germination in about 3-4 days, full colonization of the first plate, with a transfer already taken out, in about a week, and by the end of the next week there was a small cluster of knots with a pin or two already showing their color.  The PE varieties take a little longer but like I said, I'm not running out of pp5s, so I just let them hang, why not?  Might see a cool clone I want to take.  Might see a great spore drop and make some swabs.


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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Inocuole]
    #22303519 - 09/28/15 03:39 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Well, recently, with golden halo, I took spores from a swab, got germination in about 3-4 days, full colonization of the first plate, with a transfer already taken out, in about a week, and by the end of the next week there was a small cluster of knots with a pin or two already showing their color.  The PE varieties take a little longer but like I said, I'm not running out of pp5s, so I just let them hang, why not?  Might see a cool clone I want to take.  Might see a great spore drop and make some swabs.




You guys are so far ahead of me it really motivates me. With these pins and this strain of KSSS I got out of a syringe and have worked pretty hard with, I don't feel like a freshman anymore. But I am amazed at how much more I have to learn. Way, way cool for an old guy like me.

:hippie:


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