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Invisibleblackdust
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Registered: 02/28/09
Posts: 8,327
Re: Pasty Agar Tek [Re: Inocuole]
    #22192601 - 09/05/15 01:04 PM (8 years, 4 months ago)

Quote:

Inocuole said:
You wear a suit?  Heh...  I did some agar barehanded with no gloves the other day, I don't remember which though.. guess I'll see.




i think bod talks about doing that.
I did it for my last transfer as seen in the pics above
i felt like a dirty whore doing it - would not recommend


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
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Re: Pasty Agar Tek [Re: blackdust]
    #22192731 - 09/05/15 01:33 PM (8 years, 4 months ago)

Sometimes I'll go facemask and gloves only, but wipe my arms down. I don't think Muda does much more than that sometimes, although obviously his technique is better than mine.


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Invisibledreadhed
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Registered: 01/21/11
Posts: 175
Re: Pasty Agar Tek [Re: Buck513]
    #22192769 - 09/05/15 01:41 PM (8 years, 4 months ago)

Quote:

Buck513 said:
Quote:

stareatclouds said:
How much do you guys spray down your SAB before using? I usually spray Lysol on the walls, wipe them down to clean shit, and then soapy water around the sides (not the top because I can't see if I do). Then I shower and come out and use it after 20m or so. Any issues with that?



I just get a paper towel moist with alcohol, give it a good wipe down, set everything up, mist, then immediately get to work.



That's pretty much the same thing I do & it usually works out well for me. I use to use bleach water but didn't notice much difference when I switch to soap & water. Some times I still use bleach to wipe the tub down just because bleach is cheaper the rubbing alcohol.


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Offlinespacechildo
proletarians rise up
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Re: Pasty Agar Tek [Re: dreadhed]
    #22192784 - 09/05/15 01:44 PM (8 years, 4 months ago)

I use soap and water exclusively, practically free and my apt doesnt smell like shit.


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Invisiblepaperbackwriter
Edward Lear


Registered: 03/31/14
Posts: 1,888
Re: Pasty Agar Tek [Re: spacechildo]
    #22192855 - 09/05/15 01:59 PM (8 years, 4 months ago)

I use rubbing alcohol, gloves, and a dust mask.  I don't wear a shirt so it doesn't create extra drafts.


--------------------
Why should we strive with cynic frown
To knock their fairy castles down?  ~ Eliza Cook

It's rather embarrassing to have given one's entire life to pondering the human predicament and to find that in the end one has little more to say than, 'Try to be a little kinder.' ~Aldous Huxley


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InvisibleBlueIndian
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Posts: 858
Re: Pasty Agar Tek [Re: paperbackwriter]
    #22193778 - 09/05/15 05:43 PM (8 years, 4 months ago)

Quote:

paperbackwriter said:
I use rubbing alcohol, gloves, and a dust mask.  I don't wear a shirt so it doesn't create extra drafts.




:bashful:


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InvisibleBlueIndian
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Re: Pasty Agar Tek [Re: BlueIndian]
    #22213505 - 09/09/15 05:46 PM (8 years, 4 months ago)

Very nice write up Pasty! Had agar sitting for a long time just too intimidated to venture into it. I picked up some small containers but all the had were square ones....guess they should be fine. Also the agar I bought...prob 3 years ago...is yellow. Don't know how that will affect ability to watch for contams. Going to make some of these up tonight first time with agar. Question too is why not put a RTV injection port on it to not have to open it to inoculate with the lid off? Lust curious on that...

I'm not waiting days on RTV to cure on this one. Have all I need to make these up tonight...if it doesn't work out it will still be good learning experience. but rtv port would be nice in the future if using a syringe.


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OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Pasty Agar Tek [Re: BlueIndian]
    #22213542 - 09/09/15 05:54 PM (8 years, 4 months ago)

Quote:

BlueIndian said:
Question too is why not put a RTV injection port on it to not have to open it to inoculate with the lid off? Lust curious on that...



Your gonna have to open the lid anyway when transferring an agar wedge to your grain jars or another plate. Just practice good sterile procedure inside your SAB, and you won't have to worry about contams. :thumbup:


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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: Psilosoulful]
    #22213552 - 09/09/15 05:56 PM (8 years, 4 months ago)

Quote:

Psilosoulful said:
Quote:

BlueIndian said:
Question too is why not put a RTV injection port on it to not have to open it to inoculate with the lid off? Lust curious on that...



Your gonna have to open the lid anyway when transferring an agar wedge to your grain jars or another plate. Just practice good sterile procedure inside your SAB, and you won't have to worry about contams. :thumbup:




:whathesaid:


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OfflinetaGyo
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Re: Pasty Agar Tek [Re: Munchauzen]
    #22220977 - 09/11/15 09:23 AM (8 years, 4 months ago)

Just got the real deal pasty plates, the glad mini rounds :thumbup:. A lot easier to work with then the ziplocs I was using. Pics to come.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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InvisibleInocuole
Scalpel of Evil's Bane
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Re: Pasty Agar Tek [Re: taGyo]
    #22220987 - 09/11/15 09:25 AM (8 years, 4 months ago)

I do like them particularly once they've gone through a few cycles and loosened up.  Makes one handed  lid removal a breeze.  But not too much of a breeze, that would ruin the still air.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: Inocuole]
    #22222000 - 09/11/15 01:26 PM (8 years, 4 months ago)

I've tried everything else. I'm sticking with Pasty Plates (tm). They just work best.

:awesomenod:


--------------------
"He's not altogether dense, but he's not altogether there."


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Invisibleelasticaltiger
Like Tigers in Coitus
Male


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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22223023 - 09/11/15 05:04 PM (8 years, 4 months ago)

Quote:

Ajahn Don said:
I've tried everything else. I'm sticking with Pasty Plates (tm). They just work best.

:awesomenod:




:nodofunderstanding:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Invisiblestareatclouds
star eat clouds?
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Re: Pasty Agar Tek [Re: elasticaltiger]
    #22225286 - 09/12/15 12:34 AM (8 years, 4 months ago)

How do these look? PE on 3rd transfer:



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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22225295 - 09/12/15 12:35 AM (8 years, 4 months ago)

those look ready for more transfers!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblestareatclouds
star eat clouds?
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Re: Pasty Agar Tek [Re: cronicr]
    #22225311 - 09/12/15 12:41 AM (8 years, 4 months ago)

Does that mean not clean? I was planning on using them to make LI's, not isolate fully.


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22225614 - 09/12/15 03:01 AM (8 years, 4 months ago)

Nah they're clean but they're very sectored.  You already said you didn't mind that though so yeah use em if you want.  I always make a transfer to a new plate before I use a plate for something, so I don't actually lose a plate.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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Invisiblestareatclouds
star eat clouds?
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Re: Pasty Agar Tek [Re: Inocuole]
    #22225623 - 09/12/15 03:03 AM (8 years, 4 months ago)

I was considering that, but am kind of in a rush with my work right now, so didn't bother. Next time, Noc, next time.  :cool2:


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22225647 - 09/12/15 03:12 AM (8 years, 4 months ago)

Get at it.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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OfflinecronicrFacebook
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22225653 - 09/12/15 03:15 AM (8 years, 4 months ago)

Quote:

stareatclouds said:
Does that mean not clean? I was planning on using them to make LI's, not isolate fully.



yeah there clean but like inoc said some nice sectors there


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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